Rorug04G0242100

NADH-ubiquinone oxidoreductase 11 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010973
Physical Location & Seq
Forward (+)
41091074 .. 41092086
1013 bp
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UTR
Exon/CDS
Intron
Rorug04G0242100.1

Sequence Viewer

Length: 456 bp
ATGCTTGACTTTCCTTGCCTGCAGGTTGATGAGGATGATGATGAAGCTGATATTGTCAGTTGCCGCAGATTAAAAGTAGAGAATGTAAAAGCAATATCCCCTATCTTGTTAGAGTTTAGCTTCCTCGGTAAAGATTTAATCAGATATAAAAATGTTGTTGAAGTTGATCTTCCTGTTTATGAGGCAATTGTAATGTTTCGGGCTGGTAAGTGTAGCAGTGACGAACTGTTTGACAAGTTGGACACCCGTAAATTGAATGCACATTTAAAGGCACTGATGTCCGGCCTTACAGCGGAATTGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTTGAAATGATTTGTAGTCATCAGCTTGATATCGAAGAGCCTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGAGAGCGCAATGGACCTATCTGCTTGACTTCCTGA

Protein Analysis

151

Amino Acids

17.02

Weight (kDa)

4.84

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 11 - 98 4.9e-24 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 13
AciI CCGC 2 cut(s) 64, 293
AfiI CCNNNNNNNGG 1 cut(s) 292
AgsI TTSAA 4 cut(s) 161, 256, 301, 347
AluBI AGCT 3 cut(s) 47, 120, 367
AluI AGCT 3 cut(s) 47, 120, 367
Alw21I GWGCWC 1 cut(s) 394
Alw44I GTGCAC 1 cut(s) 390
AoxI GGCC 1 cut(s) 283
ApaLI GTGCAC 1 cut(s) 390
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspLEI GCGC 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 1 cut(s) 332
AvaII GGWCC 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 394
BfaI CTAG 1 cut(s) 402
BfmI CTRYAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 13
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 124, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse3DI GCAATG 1 cut(s) 436
BseDI CCNNGG 2 cut(s) 124, 336
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMI GCAATG 1 cut(s) 436
BseSI GKGCMC 1 cut(s) 394
BshFI GGCC 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 292
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 1 cut(s) 285
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 2 cut(s) 64, 293
BspANI GGCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 24
BspMI ACCTGC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 436
BssECI CCNNGG 2 cut(s) 124, 336
BssMI GATC 1 cut(s) 166
BssT1I CCWWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 228
Bst6I CTCTTC 1 cut(s) 372
BstC8I GCNNGC 1 cut(s) 20
BstF5I GGATG 1 cut(s) 40
BstHHI GCGC 1 cut(s) 429
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 20
BstSLI GKGCMC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 285
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 223, 272
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 20
CfoI GCGC 1 cut(s) 429
Cfr13I GGNCC 1 cut(s) 434
CviJI RGCY 6 cut(s) 47, 120, 203, 285, 367, 382
CviKI_1 RGCY 6 cut(s) 47, 120, 203, 285, 367, 382
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
DraI TTTAAA 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 336
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 434
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 336
FaiI YATR 4 cut(s) 147, 180, 326, 387
FalI AAGNNNNNCTT 2 cut(s) 153, 185
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 1 cut(s) 402
GlaI GCGC 1 cut(s) 428
GluI GCNGC 1 cut(s) 64
HaeIII GGCC 1 cut(s) 285
HapII CCGG 1 cut(s) 282
HhaI GCGC 1 cut(s) 429
Hin6I GCGC 1 cut(s) 427
HinP1I GCGC 1 cut(s) 427
HpaII CCGG 1 cut(s) 282
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 1 cut(s) 143
Hpy188III TCNNGA 1 cut(s) 453
Hpy8I GTNNAC 1 cut(s) 392
HpyCH4III ACNGT 1 cut(s) 228
HpyCH4V TGCA 3 cut(s) 22, 260, 392
HspAI GCGC 1 cut(s) 427
Kzo9I GATC 1 cut(s) 166
LguI GCTCTTC 1 cut(s) 372
LpnPI CCDG 5 cut(s) 8, 32, 186, 189, 295
MaeI CTAG 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 218
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 3 cut(s) 161, 389, 427
MfeI CAATTG 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 3 cut(s) 186, 251, 296
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 6 cut(s) 25, 134, 175, 310, 334, 393
MseI TTAA 3 cut(s) 71, 137, 266
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 282
MunI CAATTG 1 cut(s) 186
Mva1269I GAATGC 1 cut(s) 262
NdeII GATC 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 218
PciSI GCTCTTC 1 cut(s) 372
PctI GAATGC 1 cut(s) 262
PkrI GCNGC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 434
PstI CTGCAG 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 3 cut(s) 71, 137, 266
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 1 cut(s) 434
SbfI CCTGCAGG 1 cut(s) 24
SdaI CCTGCAGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 394
SetI ASST 6 cut(s) 27, 49, 122, 345, 369, 439
SfcI CTRYAG 1 cut(s) 20
SinI GGWCC 1 cut(s) 434
Sse8387I CCTGCAGG 1 cut(s) 24
Sse9I AATT 3 cut(s) 186, 251, 296
SsiI CCGC 2 cut(s) 64, 293
SspMI CTAG 1 cut(s) 402
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 228
TaqI TCGA 2 cut(s) 315, 375
TasI AATT 3 cut(s) 186, 251, 296
TauI GCSGC 1 cut(s) 66
Tru1I TTAA 3 cut(s) 71, 137, 266
Tru9I TTAA 3 cut(s) 71, 137, 266
TscAI CASTG 2 cut(s) 223, 279
TseFI GTSAC 1 cut(s) 218
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 1 cut(s) 57
TspRI CASTG 2 cut(s) 223, 279
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 434
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.