Rorug04G0250300

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0018186
Physical Location & Seq
Reverse (-)
42243056 .. 42245790
2735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0250300.1

Sequence Viewer

Length: 606 bp
ATGGAGAGCCAGTACATAGGAGGCAGAAGCAATACCGATGGCGGTGGGATGCCGGAGAAGGAAGTGAGGGTAGCAAACCGGAGAAGAGTTGGGAGTTGTGAGTCACTTGTGAGGGTTTTGGCCGTATTACTAACTCTGGCCGCTGCTATAGTTCAAGGGTTGAATAAGCAGACCAAAGTTGCTCCAATTAAGGTGGTTCCAACCTTGCCAGCCATTTATATTCCACTCACAGCCAAATGGCAAGACTTATCTGCTTTTGCGTACTTGCTGGTTGCAAATGTCATAGCAAGTGTATACGCAGCATTTTCCCTAGTCCTTTCCTTTGCAAACCGGGGCACAAAGAAGAGCTCACTAGGGCTCTTCATCATTGTCCTAGATATATTGATCGTGGGGGTGCTTTTCTCAGCCATCGGAGCCGCCGTGGCTATCGGTCTGATCGGGTACCATGGGAATTCACATGTGCAGTGGAACAAGGTCTGCAATGTGTTTGACAGATTTTGTCACCAGGTGATCGCATCAGTGGTCCTGACTCAGTTTGCAGCTCTTGCATTTATCTTCCTAGTTGTGCTTGCTATCTTACGCCTTAACAGAGGTCCACTTAAGTAG

Protein Analysis

201

Amino Acids

21.59

Weight (kDa)

10.02

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 28 - 182 2.9e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018186)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0431401
rosa_laevigata RLG00000006913
rosa_multiflora Rmu_sc0017459.1_g000005
rosa_roxburghii Rroxscaffold_5G00372850
rosa_rugosa Rorug04G0250300
rosa_samantha Rh4AG306200 Rh4AG306400 Rh4BG314300 Rh4BG314400 Rh4CG329900 Rh5AG135400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 294
AciI CCGC 3 cut(s) 42, 141, 417
AcoI YGGCCR 2 cut(s) 120, 138
AcsI RAATTY 1 cut(s) 451
AdeI CACNNNGTG 1 cut(s) 508
AfaI GTAC 3 cut(s) 14, 263, 443
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 457
AgsI TTSAA 2 cut(s) 155, 163
AjnI CCWGG 1 cut(s) 504
AluBI AGCT 2 cut(s) 348, 542
AluI AGCT 2 cut(s) 348, 542
Alw21I GWGCWC 1 cut(s) 350
AoxI GGCC 2 cut(s) 120, 138
ApeKI GCWGC 3 cut(s) 143, 299, 539
ApoI RAATTY 1 cut(s) 451
Asp718I GGTACC 1 cut(s) 441
AspS9I GGNCC 2 cut(s) 523, 593
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 494, 520
AvaII GGWCC 2 cut(s) 523, 593
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 441
BanII GRGCYC 2 cut(s) 350, 360
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 3 cut(s) 130, 311, 551
BccI CCATC 2 cut(s) 32, 416
BceAI ACGGC 2 cut(s) 107, 404
BciT130I CCWGG 1 cut(s) 506
BcnI CCSGG 1 cut(s) 332
BfaI CTAG 4 cut(s) 311, 353, 374, 560
BfmI CTRYAG 1 cut(s) 147
BfrI CTTAAG 1 cut(s) 599
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 5 cut(s) 141, 144, 300, 417, 540
BlsI GCNGC 5 cut(s) 142, 145, 301, 418, 541
Bme1390I CCNGG 2 cut(s) 332, 506
Bme18I GGWCC 2 cut(s) 523, 593
BmgT120I GGNCC 2 cut(s) 523, 593
BmiI GGNNCC 3 cut(s) 198, 415, 443
BmrFI CCNGG 2 cut(s) 332, 506
BmsI GCATC 2 cut(s) 39, 524
BpuMI CCSGG 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 331, 420, 445
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 3 cut(s) 26, 405, 435
Bse1I ACTGG 1 cut(s) 10
Bse3DI GCAATG 1 cut(s) 487
BseBI CCWGG 1 cut(s) 506
BseDI CCNNGG 3 cut(s) 331, 420, 445
BseGI GGATG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 2 cut(s) 417, 545
BseNI ACTGG 1 cut(s) 10
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 3 cut(s) 130, 311, 551
BsgI GTGCAG 1 cut(s) 482
BshFI GGCC 2 cut(s) 122, 140
BshNI GGYRCC 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 3 cut(s) 53, 79, 331
BsnI GGCC 2 cut(s) 122, 140
Bsp1286I GDGCHC 3 cut(s) 338, 350, 360
Bsp143I GATC 3 cut(s) 384, 435, 510
Bsp19I CCATGG 1 cut(s) 445
BspACI CCGC 3 cut(s) 42, 141, 417
BspANI GGCC 2 cut(s) 122, 140
BspCNI CTCAG 2 cut(s) 416, 544
BspLI GGNNCC 3 cut(s) 198, 415, 443
BspQI GCTCTTC 2 cut(s) 338, 365
BspT107I GGYRCC 1 cut(s) 441
BspTI CTTAAG 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 487
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 331, 420, 445
BssMI GATC 3 cut(s) 384, 435, 510
BssNAI GTATAC 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 445
Bst1107I GTATAC 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 506
Bst6I CTCTTC 3 cut(s) 79, 338, 365
BstAFI CTTAAG 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 2 cut(s) 210, 570
BstDEI CTNAG 2 cut(s) 403, 531
BstDSI CCRYGG 2 cut(s) 420, 445
BstF5I GGATG 1 cut(s) 54
BstKTI GATC 3 cut(s) 387, 438, 513
BstMBI GATC 3 cut(s) 384, 435, 510
BstMWI GCNNNNNNNGC 3 cut(s) 413, 422, 545
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 330, 504
BstSFI CTRYAG 1 cut(s) 147
BstSLI GKGCMC 1 cut(s) 338
BstV1I GCAGC 3 cut(s) 130, 311, 551
BstZ17I GTATAC 1 cut(s) 295
BsuRI GGCC 2 cut(s) 122, 140
BtgI CCRYGG 2 cut(s) 420, 445
BtsCI GGATG 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 2 cut(s) 470, 525
Cac8I GCNNGC 2 cut(s) 210, 570
Cfr13I GGNCC 2 cut(s) 523, 593
CsiI ACCWGGT 1 cut(s) 504
Csp6I GTAC 3 cut(s) 13, 262, 442
CspCI CAANNNNNGTGG 2 cut(s) 174, 209
CviAII CATG 2 cut(s) 446, 458
CviQI GTAC 3 cut(s) 13, 262, 442
DdeI CTNAG 2 cut(s) 403, 531
DpnI GATC 3 cut(s) 386, 437, 512
DpnII GATC 3 cut(s) 384, 435, 510
DraIII CACNNNGTG 1 cut(s) 508
EaeI YGGCCR 2 cut(s) 120, 138
Eam1104I CTCTTC 3 cut(s) 79, 338, 365
EarI CTCTTC 3 cut(s) 79, 338, 365
Ecl136II GAGCTC 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 2 cut(s) 350, 360
Eco47I GGWCC 2 cut(s) 523, 593
Eco53kI GAGCTC 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 2 cut(s) 350, 360
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 2 cut(s) 449, 461
FaiI YATR 8 cut(s) 17, 149, 219, 284, 295, 380, 447, 459
FatI CATG 2 cut(s) 445, 457
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 5 cut(s) 141, 144, 300, 417, 540
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 2 cut(s) 350, 360
Fsp4HI GCNGC 5 cut(s) 141, 144, 300, 417, 540
FspBI CTAG 4 cut(s) 311, 353, 374, 560
GluI GCNGC 5 cut(s) 141, 144, 300, 417, 540
HaeIII GGCC 2 cut(s) 122, 140
HapII CCGG 3 cut(s) 53, 79, 331
Hin1II CATG 2 cut(s) 449, 461
HinfI GANTC 2 cut(s) 101, 529
HpaII CCGG 3 cut(s) 53, 79, 331
HphI GGTGA 2 cut(s) 494, 520
Hpy166II GTNNAC 2 cut(s) 295, 596
Hpy188I TCNGA 2 cut(s) 413, 435
Hpy188III TCNNGA 1 cut(s) 526
Hpy8I GTNNAC 2 cut(s) 295, 596
HpyAV CCTTC 1 cut(s) 52
HpyCH4V TGCA 6 cut(s) 275, 326, 463, 480, 539, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 422, 545
HpyF3I CTNAG 2 cut(s) 403, 531
Hsp92II CATG 2 cut(s) 449, 461
KpnI GGTACC 1 cut(s) 445
Kzo9I GATC 3 cut(s) 384, 435, 510
LguI GCTCTTC 2 cut(s) 338, 365
LmnI GCTCC 2 cut(s) 187, 413
Lsp1109I GCAGC 3 cut(s) 130, 311, 551
LweI GCATC 2 cut(s) 39, 524
MabI ACCWGGT 1 cut(s) 504
MaeI CTAG 4 cut(s) 311, 353, 374, 560
MaeIII GTNAC 2 cut(s) 102, 500
MalI GATC 3 cut(s) 386, 437, 512
MboI GATC 3 cut(s) 384, 435, 510
MboII GAAGA 4 cut(s) 96, 352, 355, 547
MhlI GDGCHC 3 cut(s) 338, 350, 360
MluCI AATT 2 cut(s) 186, 451
MlyI GAGTC 2 cut(s) 110, 523
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 4 cut(s) 14, 60, 105, 584
MseI TTAA 3 cut(s) 189, 585, 600
MspA1I CMGCKG 1 cut(s) 143
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 3 cut(s) 53, 79, 331
MspR9I CCNGG 2 cut(s) 332, 506
MvaI CCWGG 1 cut(s) 506
MwoI GCNNNNNNNGC 3 cut(s) 413, 422, 545
NciI CCSGG 1 cut(s) 332
NcoI CCATGG 1 cut(s) 445
NdeII GATC 3 cut(s) 384, 435, 510
NlaIII CATG 2 cut(s) 449, 461
NlaIV GGNNCC 3 cut(s) 198, 415, 443
NmuCI GTSAC 2 cut(s) 102, 500
NspI RCATGY 1 cut(s) 461
PciI ACATGT 1 cut(s) 457
PciSI GCTCTTC 2 cut(s) 338, 365
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PkrI GCNGC 5 cut(s) 142, 145, 301, 418, 541
PleI GAGTC 2 cut(s) 109, 523
PpsI GAGTC 2 cut(s) 109, 523
PscI ACATGT 1 cut(s) 457
Psp124BI GAGCTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 3 cut(s) 198, 415, 443
PspPI GGNCC 2 cut(s) 523, 593
RsaI GTAC 3 cut(s) 14, 263, 443
RsaNI GTAC 3 cut(s) 13, 262, 442
SacI GAGCTC 1 cut(s) 350
SapI GCTCTTC 2 cut(s) 338, 365
SaqAI TTAA 3 cut(s) 189, 585, 600
SatI GCNGC 5 cut(s) 141, 144, 300, 417, 540
Sau3AI GATC 3 cut(s) 384, 435, 510
Sau96I GGNCC 2 cut(s) 523, 593
SchI GAGTC 2 cut(s) 110, 523
ScrFI CCNGG 2 cut(s) 332, 506
SduI GDGCHC 3 cut(s) 338, 350, 360
SetI ASST 7 cut(s) 195, 206, 350, 477, 510, 544, 595
SexAI ACCWGGT 1 cut(s) 504
SfaNI GCATC 2 cut(s) 39, 524
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 2 cut(s) 523, 593
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse9I AATT 2 cut(s) 186, 451
SsiI CCGC 3 cut(s) 42, 141, 417
SspMI CTAG 4 cut(s) 311, 353, 374, 560
SstI GAGCTC 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 330, 504
StyI CCWWGG 1 cut(s) 445
TaqII GACCGA 1 cut(s) 419
TasI AATT 2 cut(s) 186, 451
TatI WGTACW 1 cut(s) 12
TauI GCSGC 2 cut(s) 143, 419
Tru1I TTAA 3 cut(s) 189, 585, 600
Tru9I TTAA 3 cut(s) 189, 585, 600
TscAI CASTG 2 cut(s) 470, 525
TseFI GTSAC 2 cut(s) 102, 500
TseI GCWGC 3 cut(s) 143, 299, 539
Tsp45I GTSAC 2 cut(s) 102, 500
TspDTI ATGAA 1 cut(s) 352
TspRI CASTG 2 cut(s) 470, 525
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 523, 593
XapI RAATTY 1 cut(s) 451
XceI RCATGY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 4 cut(s) 311, 353, 374, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.