Rh4AG306200

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018186
Physical Location & Seq
Forward (+)
61823794 .. 61836781
12988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG306200.1

Sequence Viewer

Length: 957 bp
ATGATTGGATGTGAGTCTACTAGATATAGTCGTCCAGGCAAGCATATCATTCTTGCTATAAGAAGTTCCAAACGACGCCGCGTCGCTAAGCACCGTCAAAACGGCGTCGCGTCGGAGAACAACGAGAAAGCTGTCGACATCCTCTTCGTCGTCGTCGCCGGTGGCGGCGAAGTCAGAGATCTTGACTTTGATTCTTGCTTCTACAAATATGACTCCTTCATCATTGCAATCAATGGAGACTCTATCTATGATCCTACCGGAAAGATTTTCAAACACTATCTCCAGAAAAAGAACTTCGATAACACTCCCTATATAACTGCACCGCTTTTCATCTCTACTCCCATCCTTCGCTTCCATACTCATGGCCGGTGCAATTGGCGTTTGTATCCCAATTCTAGGGATGATTCCGTCTTTGCAACCGGAGAAGAATATCTTCTTCATCATCAAGGCCTTTGCCTCTGGTGTCATATTGGCCACCAGATTTATCCACATGCTTCCGGACTCTTTCGACTACTTGACCTCACCGTGCCTGAAAGAAAACCCATAGGGGAAATTACCCTTCACCGCCATCGAAACTTTGATGGTGGATTCCATATATTAATTGCTACTTCTTATTTCAAAAAGGCTCAGAATCAGATCAATGGAGAGCATGAGGGGCACATACATGTTCATACACATGCCACTCATGGTTCGCTTGATACGAATTCGGCTGAATCACAACTTCTTGGGCACAGAGTGATTTCAAAGGTTTTGGAGTTGGGAATTGTGGTGCACTCAGTTAATATTGGAATTTCATTGGGTGCTTCTGAAGGTGCTGAAACAATTCGACCTCTTCTAGTTGCTTTGACTTTCCATCAAGTTTTTGAAGGCAGGGCCATGGGACTTGGTGGTTGCATAGCTCAGGCAATTTCTCCCCCAAAATTACTGACGGTCTGTATAGTCTGTGGCCTTCTTTAG

Protein Analysis

318

Amino Acids

35.46

Weight (kDa)

8.73

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 210 - 308 2e-16 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018186)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0431401
rosa_laevigata RLG00000006913
rosa_multiflora Rmu_sc0017459.1_g000005
rosa_roxburghii Rroxscaffold_5G00372850
rosa_rugosa Rorug04G0250300
rosa_samantha Rh4AG306200 Rh4AG306400 Rh4BG314300 Rh4BG314400 Rh4CG329900 Rh5AG135400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 17, 135
AccII CGCG 2 cut(s) 81, 110
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 4 cut(s) 79, 165, 323, 565
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 2 cut(s) 364, 472
AcsI RAATTY 2 cut(s) 703, 789
AcuI CTGAAG 1 cut(s) 828
AcyI GRCGYC 2 cut(s) 76, 105
AdeI CACNNNGTG 1 cut(s) 736
AfiI CCNNNNNNNGG 1 cut(s) 396
AflIII ACRYGT 1 cut(s) 664
AgsI TTSAA 4 cut(s) 271, 619, 744, 866
AhdI GACNNNNNGTC 1 cut(s) 80
AjnI CCWGG 1 cut(s) 34
AluBI AGCT 2 cut(s) 131, 899
AluI AGCT 2 cut(s) 131, 899
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 1 cut(s) 231
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 497
AoxI GGCC 5 cut(s) 364, 448, 472, 873, 946
ApaLI GTGCAC 1 cut(s) 770
ApoI RAATTY 2 cut(s) 703, 789
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AseI ATTAAT 1 cut(s) 599
Asp700I GAANNNNTTC 2 cut(s) 432, 822
AspS9I GGNCC 1 cut(s) 873
AsuHPI GGTGA 2 cut(s) 514, 554
BaeGI GKGCMC 3 cut(s) 660, 732, 774
BalI TGGCCA 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 774
BccI CCATC 4 cut(s) 350, 575, 576, 861
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 36
BciVI GTATCC 1 cut(s) 396
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 3 cut(s) 21, 396, 836
BfuI GTATCC 1 cut(s) 396
BglII AGATCT 1 cut(s) 178
BisI GCNGC 2 cut(s) 79, 166
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 2 cut(s) 80, 167
Bme1390I CCNGG 1 cut(s) 36
BmeRI GACNNNNNGTC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 873
BmrFI CCNGG 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 266
Bpu10I CCTNAGC 1 cut(s) 900
Bpu1102I GCTNAGC 1 cut(s) 87
BsaHI GRCGYC 2 cut(s) 76, 105
BsaJI CCNNGG 1 cut(s) 876
BsaWI WCCGGW 3 cut(s) 257, 419, 497
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse118I RCCGGY 2 cut(s) 158, 366
Bse3DI GCAATG 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 876
BseGI GGATG 4 cut(s) 14, 138, 342, 406
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 3 cut(s) 641, 789, 914
BseSI GKGCMC 3 cut(s) 660, 732, 774
BsgI GTGCAG 1 cut(s) 303
Bsh1236I CGCG 2 cut(s) 81, 110
BshFI GGCC 5 cut(s) 366, 450, 474, 875, 948
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 5 cut(s) 159, 258, 367, 420, 498
BslFI GGGAC 1 cut(s) 894
BslI CCNNNNNNNGG 1 cut(s) 396
BsmAI GTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 894
BsnI GGCC 5 cut(s) 366, 450, 474, 875, 948
Bsp1286I GDGCHC 3 cut(s) 660, 732, 774
Bsp13I TCCGGA 1 cut(s) 497
Bsp143I GATC 3 cut(s) 178, 250, 636
Bsp1720I GCTNAGC 1 cut(s) 87
Bsp19I CCATGG 1 cut(s) 876
BspACI CCGC 4 cut(s) 79, 165, 323, 565
BspANI GGCC 5 cut(s) 366, 450, 474, 875, 948
BspCNI CTCAG 3 cut(s) 640, 788, 913
BspEI TCCGGA 1 cut(s) 497
BspFNI CGCG 2 cut(s) 81, 110
BspPI GGATC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 2 cut(s) 158, 366
BssAI RCCGGY 2 cut(s) 158, 366
BssECI CCNNGG 1 cut(s) 876
BssMI GATC 3 cut(s) 178, 250, 636
BssNI GRCGYC 2 cut(s) 76, 105
BssT1I CCWWGG 1 cut(s) 876
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 3 cut(s) 95, 526, 931
Bst6I CTCTTC 2 cut(s) 149, 837
BstACI GRCGYC 2 cut(s) 76, 105
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 4 cut(s) 87, 627, 775, 900
BstDSI CCRYGG 1 cut(s) 876
BstF5I GGATG 4 cut(s) 14, 138, 342, 406
BstFNI CGCG 2 cut(s) 81, 110
BstKTI GATC 3 cut(s) 181, 253, 639
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 3 cut(s) 178, 250, 636
BstMWI GCNNNNNNNGC 1 cut(s) 655
BstNI CCWGG 1 cut(s) 36
BstNSI RCATGY 3 cut(s) 494, 668, 680
BstSCI CCNGG 1 cut(s) 34
BstSLI GKGCMC 3 cut(s) 660, 732, 774
BstUI CGCG 2 cut(s) 81, 110
BstX2I RGATCY 1 cut(s) 178
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 1 cut(s) 178
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 5 cut(s) 366, 450, 474, 875, 948
BtgI CCRYGG 1 cut(s) 876
BtsCI GGATG 4 cut(s) 14, 138, 342, 406
Cac8I GCNNGC 1 cut(s) 41
Cfr10I RCCGGY 2 cut(s) 158, 366
Cfr13I GGNCC 1 cut(s) 873
CseI GACGC 4 cut(s) 70, 84, 94, 99
CviAII CATG 7 cut(s) 362, 491, 650, 665, 677, 686, 877
CviJI RGCY 9 cut(s) 131, 366, 450, 474, 626, 710, 875, 899, 948
CviKI_1 RGCY 9 cut(s) 131, 366, 450, 474, 626, 710, 875, 899, 948
DdeI CTNAG 4 cut(s) 87, 627, 775, 900
DpnI GATC 3 cut(s) 180, 252, 638
DpnII GATC 3 cut(s) 178, 250, 636
DraIII CACNNNGTG 1 cut(s) 736
DriI GACNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 2 cut(s) 364, 472
Eam1104I CTCTTC 2 cut(s) 149, 837
Eam1105I GACNNNNNGTC 1 cut(s) 80
EarI CTCTTC 2 cut(s) 149, 837
Eco130I CCWWGG 1 cut(s) 876
Eco147I AGGCCT 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 828
EcoRI GAATTC 1 cut(s) 703
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 7 cut(s) 365, 494, 653, 668, 680, 689, 880
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 894
FatI CATG 7 cut(s) 361, 490, 649, 664, 676, 685, 876
FblI GTMKAC 2 cut(s) 17, 135
Fnu4HI GCNGC 2 cut(s) 79, 166
FokI GGATG 4 cut(s) 21, 125, 329, 413
Fsp4HI GCNGC 2 cut(s) 79, 166
FspBI CTAG 3 cut(s) 21, 396, 836
GluI GCNGC 2 cut(s) 79, 166
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 5 cut(s) 366, 450, 474, 875, 948
HapII CCGG 5 cut(s) 159, 258, 367, 420, 498
HgaI GACGC 4 cut(s) 70, 84, 94, 99
Hin1I GRCGYC 2 cut(s) 76, 105
Hin1II CATG 7 cut(s) 365, 494, 653, 668, 680, 689, 880
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 9 cut(s) 14, 191, 212, 239, 404, 501, 588, 631, 713
HpaII CCGG 5 cut(s) 159, 258, 367, 420, 498
HphI GGTGA 2 cut(s) 514, 554
Hpy166II GTNNAC 3 cut(s) 18, 136, 772
Hpy188I TCNGA 5 cut(s) 115, 176, 630, 636, 808
Hpy188III TCNNGA 3 cut(s) 182, 283, 498
Hpy8I GTNNAC 3 cut(s) 18, 136, 772
Hpy99I CGWCG 7 cut(s) 78, 86, 110, 115, 152, 155, 158
HpyAV CCTTC 5 cut(s) 226, 356, 569, 803, 860
HpyCH4III ACNGT 3 cut(s) 95, 526, 931
HpyCH4V TGCA 6 cut(s) 227, 320, 372, 416, 772, 894
HpyF10VI GCNNNNNNNGC 1 cut(s) 655
HpyF3I CTNAG 4 cut(s) 87, 627, 775, 900
Hsp92I GRCGYC 2 cut(s) 76, 105
Hsp92II CATG 7 cut(s) 365, 494, 653, 668, 680, 689, 880
Kpn2I TCCGGA 1 cut(s) 497
Kzo9I GATC 3 cut(s) 178, 250, 636
MaeI CTAG 3 cut(s) 21, 396, 836
MalI GATC 3 cut(s) 180, 252, 638
MboI GATC 3 cut(s) 178, 250, 636
MboII GAAGA 5 cut(s) 136, 425, 428, 437, 824
MfeI CAATTG 1 cut(s) 373
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 3 cut(s) 660, 732, 774
MlsI TGGCCA 1 cut(s) 474
MluNI TGGCCA 1 cut(s) 474
MlyI GAGTC 4 cut(s) 23, 206, 233, 495
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 5 cut(s) 152, 467, 530, 646, 840
Mox20I TGGCCA 1 cut(s) 474
MroI TCCGGA 1 cut(s) 497
MroXI GAANNNNTTC 2 cut(s) 432, 822
MscI TGGCCA 1 cut(s) 474
MseI TTAA 2 cut(s) 599, 780
MslI CAYNNNNRTG 3 cut(s) 360, 663, 675
Msp20I TGGCCA 1 cut(s) 474
MspI CCGG 5 cut(s) 159, 258, 367, 420, 498
MspR9I CCNGG 1 cut(s) 36
MunI CAATTG 1 cut(s) 373
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 2 cut(s) 81, 110
MwoI GCNNNNNNNGC 1 cut(s) 655
NcoI CCATGG 1 cut(s) 876
NdeII GATC 3 cut(s) 178, 250, 636
NlaIII CATG 7 cut(s) 365, 494, 653, 668, 680, 689, 880
NspI RCATGY 3 cut(s) 494, 668, 680
PceI AGGCCT 1 cut(s) 450
PciI ACATGT 1 cut(s) 664
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PdmI GAANNNNTTC 2 cut(s) 432, 822
PfeI GAWTC 5 cut(s) 191, 404, 588, 631, 713
PkrI GCNGC 2 cut(s) 80, 167
PleI GAGTC 4 cut(s) 22, 206, 233, 495
PpsI GAGTC 4 cut(s) 22, 206, 233, 495
PscI ACATGT 1 cut(s) 664
PshBI ATTAAT 1 cut(s) 599
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspPI GGNCC 1 cut(s) 873
PsuI RGATCY 1 cut(s) 178
RseI CAYNNNNRTG 3 cut(s) 360, 663, 675
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 2 cut(s) 599, 780
SatI GCNGC 2 cut(s) 79, 166
Sau3AI GATC 3 cut(s) 178, 250, 636
Sau96I GGNCC 1 cut(s) 873
SchI GAGTC 4 cut(s) 23, 206, 233, 495
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 3 cut(s) 660, 732, 774
SetI ASST 6 cut(s) 133, 522, 750, 814, 832, 901
SgrAI CRCCGGYG 1 cut(s) 158
SmiMI CAYNNNNRTG 3 cut(s) 360, 663, 675
SseBI AGGCCT 1 cut(s) 450
SsiI CCGC 4 cut(s) 79, 165, 323, 565
SspI AATATT 1 cut(s) 784
SspMI CTAG 3 cut(s) 21, 396, 836
StuI AGGCCT 1 cut(s) 450
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 3 cut(s) 95, 526, 931
TaqI TCGA 5 cut(s) 135, 297, 508, 571, 826
TauI GCSGC 2 cut(s) 81, 168
TfiI GAWTC 5 cut(s) 191, 404, 588, 631, 713
Tru1I TTAA 2 cut(s) 599, 780
Tru9I TTAA 2 cut(s) 599, 780
TspDTI ATGAA 5 cut(s) 208, 319, 428, 659, 783
TspGWI ACGGA 1 cut(s) 397
VneI GTGCAC 1 cut(s) 770
VspI ATTAAT 1 cut(s) 599
XapI RAATTY 2 cut(s) 703, 789
XceI RCATGY 3 cut(s) 494, 668, 680
XmiI GTMKAC 2 cut(s) 17, 135
XmnI GAANNNNTTC 2 cut(s) 432, 822
XspI CTAG 3 cut(s) 21, 396, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.