Rorug04G0297100
BZIP Family

Ribonuclease P protein subunit p25-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012846
Physical Location & Seq
Forward (+)
47172961 .. 47175495
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0297100.1

Sequence Viewer

Length: 1602 bp
ATGTCTAGAAGAGTTCCTGGTTATGATGAGCAAAAGGCTGCGAGGGAGTTTGCAAATTTTTCTTGCTCTGCAGACTTTCCACCTCAGATGGTTGAAGATAGACCTTTTAGAGGCTTTCTAGAGTCGCTGAATCCGCAGTTTAAGCTCAGTTTCAATAGGGTGGGGAGTGAATGCATGCAAGTCTATAAGGAAAGGAAAGCAGAAACCAAGAAATTCTTAGTAAACTTCGATGGACGGATTAGCCTCTCCATGGACATATTGAGGCACGAAAGTGGCCGTTGGGATACATTTCGCTGTGATGATTACTTATGCCTTTCTGCACACTTTATTGATGAGAATTGGAAACTGAAAAAGTGGGTTCTTAGCTTCCGGAGTCTTTGGGGTTCGGATGAGTCTGATGATTACATTCCTGAAGGCGATGATTGGGGCATTTTTAAGTTGCTTCAAGATTGGGGAATTCAGAACAAGATATCCACTCTCACTAGGAATAATGAGGATTGCGATGATTCATTGGCTGAGTTTGTGAAAAGTCACATCCAGGACAAGAATCTCCAACTCAATGGTCAGCTGTTTCGTGTGCATTGTTGTGGGGACATGATCAAGACAATGGTGGAGGATGCATTTGAAATGATTAAAGATATCATTTACAAAGTCCGTCAGCTGTATTCTTTTGGGAGGTCATTACCCCTGTGGTATCTCACAACTCACCACCTAAAAGGAGCTCTGCAGCTATGGTCTATGGGAGAGTTCTCTTCACAAGATGTGATTAATGATTACGATGTACCACCCCCTGAGGAATGGAAGAAAGTTGAAGGTGTTTGTTTAATTGCTGACAAGATGTATGAAGTATCAAATGCATTGTTTGAAACCAAGCATCTAACATGTAATGTTTATCTTTATCACCTTGATGAGCTTCGCGAAATTCTGATCCAATCTTCCACCGACTCTGATGAGTTCGTTGCAACATTAGCCAAGGACATGTTGAAGAAGTTTGACAAGTATTGGGATGACATGTTCTTGCTGTTGGCAATATCTGCAGCGCTGGATCCTCGGTTTAAGATGAAATACATAGATTTTGTTTGTTCAAAAGTTAAGGGTATGGAAGGAAGCTCGCAAGCTGCAGCTGTTTTGGTGTCTATGAACAAACTATTTGATGAATATGTGATCCGCTTTCCTGAGAAAGAGAACTTTATATGTGAATCTTCTTCTGATTCTGATTCTGATTCTGGTTCTGATTCAGAAGGAGAGTGTCCTACTGCTGGATGTGCGAATCATACTTTTAGTGTGGTGAAGAACTACGAGCAATTCCTCCAGTTAAGTGATCAGCCTGCAAAGAAATCAGACTTGCATTTCTATTTGGAACAGCCTGTCTTACCTTGGAGTCAGGATTTCGATGCATTGACTTGGTGGAGCACTGCAGGGGCCAGGTATCCTATCCTTTCCAGGATGGCACGCGATTTCTTGGCTATTCCAGTTTCTGTTGCCTCTTCATATGAGGCATTCTATACTGAAGCAAGGCCAGCTGATGAACACGTTGTTTGCTTGAAGCCAGAGTTGATGAATGCCCTAGTGTGTTCTCGAAGCTGGTATTCTAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko03013 map03008 map03013
Pfam Domains
Protein Families
Alba

Protein Analysis

533

Amino Acids

61.67

Weight (kDa)

5.03

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
hAT-like_RNase-H PF14372 290 - 387 6.9e-23 hAT-like transposase, RNase-H fold
Dimer_Tnp_hAT PF05699 449 - 530 9.6e-24 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012846)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13600 AT2G04038
fragaria_vesca FvH4_4g28780
malus_domestica MD13G1053800.v1.1 MD16G1052300.v1.1
prunus_persica Prupe.1G298200_v2.0.a1
pyrus_communis pycom13g04800 pycom16g04590
rosa_chinensis RchiOBHm_Chr4g0436911
rosa_laevigata RLG00000006488
rosa_multiflora Rmu_sc0001464.1_g000012
rosa_roxburghii Rroxscaffold_5G00377960
rosa_rugosa Rorug04G0297100
rosa_samantha Rh4AG351600 Rh4BG360400 Rh4CG374700 Rh4DG354800
rosa_wichuraiana Rw4G030770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 918, 1455
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 2 cut(s) 134, 1168
AclWI GGATC 4 cut(s) 922, 1040, 1053, 1159
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 4 cut(s) 55, 212, 456, 921
AcuI CTGAAG 2 cut(s) 432, 1530
AfaI GTAC 1 cut(s) 783
AfeI AGCGCT 1 cut(s) 1041
AfiI CCNNNNNNNGG 3 cut(s) 110, 250, 1259
AflIII ACRYGT 4 cut(s) 881, 978, 1011, 1531
AgsI TTSAA 9 cut(s) 95, 154, 446, 626, 812, 866, 985, 1086, 1546
AjnI CCWGG 4 cut(s) 16, 537, 1424, 1442
AleI CACNNNNGTG 1 cut(s) 270
AloI GAACNNNNNNTCC 4 cut(s) 455, 487, 998, 1030
Alw21I GWGCWC 2 cut(s) 724, 1415
AlwI GGATC 4 cut(s) 922, 1040, 1053, 1159
AlwNI CAGNNNCTG 1 cut(s) 1478
Aor13HI TCCGGA 1 cut(s) 369
Aor51HI AGCGCT 1 cut(s) 1041
AoxI GGCC 3 cut(s) 274, 1422, 1517
ApeKI GCWGC 5 cut(s) 38, 727, 1037, 1118, 1121
ApoI RAATTY 4 cut(s) 55, 212, 456, 921
AseI ATTAAT 1 cut(s) 768
AspLEI GCGC 1 cut(s) 1042
AspS9I GGNCC 1 cut(s) 1422
AsuHPI GGTGA 3 cut(s) 698, 893, 1300
AxyI CCTNAGG 1 cut(s) 792
BamHI GGATCC 1 cut(s) 1045
BanII GRGCYC 1 cut(s) 724
Bbv12I GWGCWC 2 cut(s) 724, 1415
BbvI GCAGC 5 cut(s) 25, 739, 1049, 1105, 1133
BccI CCATC 3 cut(s) 82, 224, 1441
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 4 cut(s) 18, 539, 1426, 1444
BciVI GTATCC 2 cut(s) 277, 1440
BclI TGATCA 2 cut(s) 597, 1321
BfaI CTAG 4 cut(s) 6, 119, 483, 1568
BfmI CTRYAG 5 cut(s) 69, 725, 1035, 1119, 1416
BfoI RGCGCY 1 cut(s) 1043
BfuI GTATCC 2 cut(s) 277, 1440
BisI GCNGC 5 cut(s) 39, 728, 1038, 1119, 1122
BlsI GCNGC 5 cut(s) 40, 729, 1039, 1120, 1123
Bme1390I CCNGG 4 cut(s) 18, 539, 1426, 1444
BmgT120I GGNCC 1 cut(s) 1422
BmiI GGNNCC 2 cut(s) 1047, 1423
BmrFI CCNGG 4 cut(s) 18, 539, 1426, 1444
BmsI GCATC 3 cut(s) 607, 883, 1384
BpmI CTGGAG 1 cut(s) 1295
BsaJI CCNNGG 4 cut(s) 249, 972, 1049, 1376
BsaWI WCCGGW 1 cut(s) 369
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 250, 1259
Bse1I ACTGG 2 cut(s) 1312, 1472
Bse21I CCTNAGG 1 cut(s) 792
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 4 cut(s) 18, 539, 1426, 1444
BseDI CCNNGG 4 cut(s) 249, 972, 1049, 1376
BseGI GGATG 6 cut(s) 394, 534, 622, 1012, 1268, 1452
BseLI CCNNNNNNNGG 3 cut(s) 110, 250, 1259
BseMII CTCAG 5 cut(s) 98, 160, 507, 783, 1167
BseNI ACTGG 2 cut(s) 1312, 1472
BseXI GCAGC 5 cut(s) 25, 739, 1049, 1105, 1133
BsgI GTGCAG 1 cut(s) 303
Bsh1236I CGCG 2 cut(s) 918, 1455
BshFI GGCC 3 cut(s) 276, 1424, 1519
BsiHKAI GWGCWC 2 cut(s) 724, 1415
BsiSI CCGG 1 cut(s) 370
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 3 cut(s) 110, 250, 1259
BsmFI GGGAC 1 cut(s) 605
BsmI GAATGC 3 cut(s) 176, 1499, 1567
BsnI GGCC 3 cut(s) 276, 1424, 1519
Bsp1286I GDGCHC 2 cut(s) 724, 1415
Bsp13I TCCGGA 1 cut(s) 369
Bsp143I GATC 5 cut(s) 597, 927, 1045, 1164, 1321
Bsp19I CCATGG 1 cut(s) 249
Bsp68I TCGCGA 1 cut(s) 918
BspACI CCGC 2 cut(s) 134, 1168
BspANI GGCC 3 cut(s) 276, 1424, 1519
BspCNI CTCAG 5 cut(s) 97, 159, 508, 784, 1168
BspEI TCCGGA 1 cut(s) 369
BspFNI CGCG 2 cut(s) 918, 1455
BspLI GGNNCC 2 cut(s) 1047, 1423
BspMAI CTGCAG 5 cut(s) 73, 729, 1039, 1123, 1420
BspPI GGATC 4 cut(s) 922, 1040, 1053, 1159
BsrI ACTGG 2 cut(s) 1312, 1472
BssECI CCNNGG 4 cut(s) 249, 972, 1049, 1376
BssMI GATC 5 cut(s) 597, 927, 1045, 1164, 1321
BssT1I CCWWGG 3 cut(s) 249, 972, 1376
Bst2UI CCWGG 4 cut(s) 18, 539, 1426, 1444
Bst6I CTCTTC 3 cut(s) 4, 757, 1492
BstAPI GCANNNNNTGC 1 cut(s) 1034
BstC8I GCNNGC 6 cut(s) 176, 1112, 1116, 1329, 1453, 1521
BstDEI CTNAG 8 cut(s) 84, 146, 217, 362, 516, 792, 1176, 1593
BstDSI CCRYGG 1 cut(s) 249
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 6 cut(s) 394, 534, 622, 1012, 1268, 1452
BstFNI CGCG 2 cut(s) 918, 1455
BstH2I RGCGCY 1 cut(s) 1043
BstHHI GCGC 1 cut(s) 1042
BstKTI GATC 5 cut(s) 600, 930, 1048, 1167, 1324
BstMBI GATC 5 cut(s) 597, 927, 1045, 1164, 1321
BstMWI GCNNNNNNNGC 6 cut(s) 133, 142, 968, 1034, 1265, 1520
BstNI CCWGG 4 cut(s) 18, 539, 1426, 1444
BstNSI RCATGY 4 cut(s) 178, 885, 982, 1015
BstSCI CCNGG 4 cut(s) 16, 537, 1424, 1442
BstSFI CTRYAG 5 cut(s) 69, 725, 1035, 1119, 1416
BstUI CGCG 2 cut(s) 918, 1455
BstV1I GCAGC 5 cut(s) 25, 739, 1049, 1105, 1133
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 1 cut(s) 560
BstYI RGATCY 1 cut(s) 1045
Bsu36I CCTNAGG 1 cut(s) 792
BsuI GTATCC 2 cut(s) 277, 1440
BsuRI GGCC 3 cut(s) 276, 1424, 1519
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 2 cut(s) 432, 516
BtsCI GGATG 6 cut(s) 394, 534, 622, 1012, 1268, 1452
BtsI GCAGTG 1 cut(s) 1413
BtsIMutI CAGTG 2 cut(s) 1413, 1597
BtuMI TCGCGA 1 cut(s) 918
Cac8I GCNNGC 6 cut(s) 176, 1112, 1116, 1329, 1453, 1521
CaiI CAGNNNCTG 1 cut(s) 1478
CfoI GCGC 1 cut(s) 1042
Cfr13I GGNCC 1 cut(s) 1422
Csp6I GTAC 1 cut(s) 782
CviAII CATG 6 cut(s) 175, 250, 595, 882, 979, 1012
CviQI GTAC 1 cut(s) 782
DdeI CTNAG 8 cut(s) 84, 146, 217, 362, 516, 792, 1176, 1593
DpnI GATC 5 cut(s) 599, 929, 1047, 1166, 1323
DpnII GATC 5 cut(s) 597, 927, 1045, 1164, 1321
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 3 cut(s) 4, 757, 1492
EarI CTCTTC 3 cut(s) 4, 757, 1492
Ecl136II GAGCTC 1 cut(s) 722
Eco130I CCWWGG 3 cut(s) 249, 972, 1376
Eco24I GRGCYC 1 cut(s) 724
Eco32I GATATC 2 cut(s) 471, 640
Eco47III AGCGCT 1 cut(s) 1041
Eco53kI GAGCTC 1 cut(s) 722
Eco57I CTGAAG 2 cut(s) 432, 1530
Eco81I CCTNAGG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 722
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoRI GAATTC 1 cut(s) 456
EcoRII CCWGG 4 cut(s) 16, 537, 1424, 1442
EcoRV GATATC 2 cut(s) 471, 640
EcoT14I CCWWGG 3 cut(s) 249, 972, 1376
EcoT22I ATGCAT 4 cut(s) 176, 622, 859, 1399
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 3 cut(s) 249, 972, 1376
FaeI CATG 6 cut(s) 178, 253, 598, 885, 982, 1015
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 605
FatI CATG 6 cut(s) 174, 249, 594, 881, 978, 1011
FauNDI CATATG 1 cut(s) 1492
FbaI TGATCA 2 cut(s) 597, 1321
Fnu4HI GCNGC 5 cut(s) 39, 728, 1038, 1119, 1122
FokI GGATG 6 cut(s) 401, 521, 629, 1019, 1275, 1459
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 5 cut(s) 39, 728, 1038, 1119, 1122
FspBI CTAG 4 cut(s) 6, 119, 483, 1568
GlaI GCGC 1 cut(s) 1041
GluI GCNGC 5 cut(s) 39, 728, 1038, 1119, 1122
GsuI CTGGAG 1 cut(s) 1295
HaeII RGCGCY 1 cut(s) 1043
HaeIII GGCC 3 cut(s) 276, 1424, 1519
HapII CCGG 1 cut(s) 370
HhaI GCGC 1 cut(s) 1042
Hin1II CATG 6 cut(s) 178, 253, 598, 885, 982, 1015
Hin6I GCGC 1 cut(s) 1040
HinP1I GCGC 1 cut(s) 1040
HpaII CCGG 1 cut(s) 370
HphI GGTGA 3 cut(s) 698, 893, 1300
Hpy166II GTNNAC 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 223
HpyAV CCTTC 4 cut(s) 407, 806, 1097, 1235
HpyCH4IV ACGT 1 cut(s) 1533
HpyF10VI GCNNNNNNNGC 6 cut(s) 133, 142, 968, 1034, 1265, 1520
HpyF3I CTNAG 8 cut(s) 84, 146, 217, 362, 516, 792, 1176, 1593
HpySE526I ACGT 1 cut(s) 1533
Hsp92II CATG 6 cut(s) 178, 253, 598, 885, 982, 1015
HspAI GCGC 1 cut(s) 1040
Kpn2I TCCGGA 1 cut(s) 369
Ksp22I TGATCA 2 cut(s) 597, 1321
Kzo9I GATC 5 cut(s) 597, 927, 1045, 1164, 1321
LmnI GCTCC 2 cut(s) 719, 1410
Lsp1109I GCAGC 5 cut(s) 25, 739, 1049, 1105, 1133
LweI GCATC 3 cut(s) 607, 883, 1384
MaeI CTAG 4 cut(s) 6, 119, 483, 1568
MaeII ACGT 1 cut(s) 1533
MaeIII GTNAC 1 cut(s) 530
MalI GATC 5 cut(s) 599, 929, 1047, 1166, 1323
MboI GATC 5 cut(s) 597, 927, 1045, 1164, 1321
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 2 cut(s) 724, 1415
MluCI AATT 7 cut(s) 55, 212, 337, 456, 825, 921, 1304
MlyI GAGTC 5 cut(s) 131, 382, 401, 938, 1390
MmeI TCCRAC 1 cut(s) 577
Mph1103I ATGCAT 4 cut(s) 176, 622, 859, 1399
MroI TCCGGA 1 cut(s) 369
MseI TTAA 8 cut(s) 141, 435, 633, 768, 824, 1056, 1092, 1316
MslI CAYNNNNRTG 3 cut(s) 270, 585, 906
MspA1I CMGCKG 4 cut(s) 568, 661, 1124, 1523
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 4 cut(s) 18, 539, 1426, 1444
Mva1269I GAATGC 3 cut(s) 176, 1499, 1567
MvaI CCWGG 4 cut(s) 18, 539, 1426, 1444
MvnI CGCG 2 cut(s) 918, 1455
MwoI GCNNNNNNNGC 6 cut(s) 133, 142, 968, 1034, 1265, 1520
NcoI CCATGG 1 cut(s) 249
NdeI CATATG 1 cut(s) 1492
NdeII GATC 5 cut(s) 597, 927, 1045, 1164, 1321
NlaIII CATG 6 cut(s) 178, 253, 598, 885, 982, 1015
NlaIV GGNNCC 2 cut(s) 1047, 1423
NmuCI GTSAC 1 cut(s) 530
NruI TCGCGA 1 cut(s) 918
NsiI ATGCAT 4 cut(s) 176, 622, 859, 1399
NspI RCATGY 4 cut(s) 178, 885, 982, 1015
OliI CACNNNNGTG 1 cut(s) 270
PaeI GCATGC 1 cut(s) 178
PciI ACATGT 3 cut(s) 881, 978, 1011
PctI GAATGC 3 cut(s) 176, 1499, 1567
PfeI GAWTC 9 cut(s) 130, 506, 547, 1199, 1211, 1217, 1223, 1235, 1270
PfoI TCCNGGA 2 cut(s) 537, 1442
PkrI GCNGC 5 cut(s) 40, 729, 1039, 1120, 1123
PleI GAGTC 5 cut(s) 130, 381, 400, 938, 1389
PpsI GAGTC 5 cut(s) 130, 381, 400, 938, 1389
PscI ACATGT 3 cut(s) 881, 978, 1011
PshBI ATTAAT 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 724
Psp6I CCWGG 4 cut(s) 16, 537, 1424, 1442
PspGI CCWGG 4 cut(s) 16, 537, 1424, 1442
PspN4I GGNNCC 2 cut(s) 1047, 1423
PspPI GGNCC 1 cut(s) 1422
PstI CTGCAG 5 cut(s) 73, 729, 1039, 1123, 1420
PstNI CAGNNNCTG 1 cut(s) 1478
PsuI RGATCY 1 cut(s) 1045
PvuII CAGCTG 4 cut(s) 568, 661, 1124, 1523
RruI TCGCGA 1 cut(s) 918
RsaI GTAC 1 cut(s) 783
RsaNI GTAC 1 cut(s) 782
RseI CAYNNNNRTG 3 cut(s) 270, 585, 906
SacI GAGCTC 1 cut(s) 724
SaqAI TTAA 8 cut(s) 141, 435, 633, 768, 824, 1056, 1092, 1316
SatI GCNGC 5 cut(s) 39, 728, 1038, 1119, 1122
Sau3AI GATC 5 cut(s) 597, 927, 1045, 1164, 1321
Sau96I GGNCC 1 cut(s) 1422
SchI GAGTC 5 cut(s) 131, 382, 401, 938, 1390
ScrFI CCNGG 4 cut(s) 18, 539, 1426, 1444
SduI GDGCHC 2 cut(s) 724, 1415
SfaNI GCATC 3 cut(s) 607, 883, 1384
SfcI CTRYAG 5 cut(s) 69, 725, 1035, 1119, 1416
SmiMI CAYNNNNRTG 3 cut(s) 270, 585, 906
SphI GCATGC 1 cut(s) 178
Sse9I AATT 7 cut(s) 55, 212, 337, 456, 825, 921, 1304
SsiI CCGC 2 cut(s) 134, 1168
SspMI CTAG 4 cut(s) 6, 119, 483, 1568
SstI GAGCTC 1 cut(s) 724
StyD4I CCNGG 4 cut(s) 16, 537, 1424, 1442
StyI CCWWGG 3 cut(s) 249, 972, 1376
TaiI ACGT 1 cut(s) 1536
TaqI TCGA 3 cut(s) 228, 1392, 1579
TasI AATT 7 cut(s) 55, 212, 337, 456, 825, 921, 1304
TfiI GAWTC 9 cut(s) 130, 506, 547, 1199, 1211, 1217, 1223, 1235, 1270
Tru1I TTAA 8 cut(s) 141, 435, 633, 768, 824, 1056, 1092, 1316
Tru9I TTAA 8 cut(s) 141, 435, 633, 768, 824, 1056, 1092, 1316
TscAI CASTG 1 cut(s) 1420
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 5 cut(s) 38, 727, 1037, 1118, 1121
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 8 cut(s) 498, 858, 1076, 1154, 1170, 1479, 1542, 1574
TspGWI ACGGA 2 cut(s) 250, 644
TspRI CASTG 1 cut(s) 1420
VspI ATTAAT 1 cut(s) 768
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 4 cut(s) 55, 212, 456, 921
XbaI TCTAGA 2 cut(s) 5, 118
XceI RCATGY 4 cut(s) 178, 885, 982, 1015
XspI CTAG 4 cut(s) 6, 119, 483, 1568
Zsp2I ATGCAT 4 cut(s) 176, 622, 859, 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.