Rorug04G0326400

Single-stranded DNA-binding protein WHY1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009517
Physical Location & Seq
Reverse (-)
50145123 .. 50147874
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0326400.1

Sequence Viewer

Length: 609 bp
ATGCAGTTCTCTCAGGACGACTTCGACCGCCTCTTGCTCTCCGAGCACAGCCGCAAGACCTGCGAAGCTCACTACGCCAAGAACCCTCAAGACGCCGAGAACTTAGTCAAATGGGCCGGAGCTCTGCTCGAACTGTCGCAGTTTCAGAGTATTGCCGACTCCCGGATCATGCTTGATGATTGTATTTCGAAGCTGGAGGAGGCTCTGGCGATCAATCCTACAAAGCATGAAGCTCTATGGTGTCTTGGGAACGCGCATACATCTCATGGATTCATGAATCCTGACTTGGACGAGGCAAGGCCTTATTTCGAGAAGGCGTCGGAGTATTTCCAGAGGGCTGCCGAGCAGGAACCTGCCAATGAGCTTTATCAGAAGTCCATAGAAGTCACAGCTAAGGCACCAGAGTTGCATATGGAGATCCATAAGCAAGGAATGGCTCAACAAGCACTAGGTGGGGGACCGGCCACTTCCTCAAGTGGAAAGAGTTCCAAGGCTAAGAGGAGCAGTGATCTCAAGTATGACATATGTGGATGGATTATCCTTGCAGTTGGCATTTTTGCATGGGTTGGAGCGAAATCCAACGTGCCACCACCCCCACCCCCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000229 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0008150 GO:0008152 GO:0009295 GO:0009507 GO:0009508 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042162 GO:0042646 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045893 GO:0045910 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1904356 GO:1904357 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Whirly

Protein Analysis

202

Amino Acids

22.4

Weight (kDa)

5.64

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOM20_plant PF06552 8 - 191 3.2e-87 Plant specific mitochondrial import receptor subunit TOM20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009517)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14410 AT2G02740
fragaria_vesca FvH4_4g31850 FvH4_4g31850
malus_domestica MD13G1078000.v1.1 MD16G1077800.v1.1
prunus_persica Prupe.1G269100_v2.0.a1
pyrus_communis pycom16g06720
rosa_chinensis RchiOBHm_Chr4g0440511
rosa_laevigata RLG00000006175
rosa_multiflora Rmu_sc0007648.1_g000008
rosa_roxburghii Rroxscaffold_5G00381340
rosa_rugosa Rorug04G0326400
rosa_samantha Rh4AG378000 Rh4BG390600 Rh4CG405300 Rh4DG384300 Rh5DG089700
rosa_wichuraiana Rw0G019510 Rw4G032300 Rw4G032350 Rw4G032450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 68, 361
AccB1I GGYRCC 1 cut(s) 397
AccII CGCG 1 cut(s) 254
AciI CCGC 2 cut(s) 28, 52
AclWI GGATC 2 cut(s) 173, 412
AcoI YGGCCR 1 cut(s) 462
AcyI GRCGYC 2 cut(s) 93, 317
AdeI CACNNNGTG 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 162
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AluBI AGCT 6 cut(s) 68, 122, 193, 233, 364, 392
AluI AGCT 6 cut(s) 68, 122, 193, 233, 364, 392
Alw21I GWGCWC 2 cut(s) 48, 124
AlwI GGATC 2 cut(s) 173, 412
AoxI GGCC 3 cut(s) 114, 299, 462
ApeKI GCWGC 1 cut(s) 338
Asp700I GAANNNNTTC 1 cut(s) 484
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 2 cut(s) 114, 458
AsuC2I CCSGG 1 cut(s) 163
AsuII TTCGAA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 458
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 124
Bbv12I GWGCWC 2 cut(s) 48, 124
BbvI GCAGC 1 cut(s) 325
BccI CCATC 1 cut(s) 525
BcnI CCSGG 1 cut(s) 163
BfaI CTAG 1 cut(s) 449
BfuAI ACCTGC 2 cut(s) 68, 361
BisI GCNGC 2 cut(s) 52, 339
BlsI GCNGC 2 cut(s) 53, 340
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 114, 458
BmiI GGNNCC 3 cut(s) 351, 399, 459
BmrFI CCNGG 1 cut(s) 163
BplI GAGNNNNNCTC 2 cut(s) 111, 143
BpmI CTGGAG 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 3 cut(s) 72, 457, 497
BpuMI CCSGG 1 cut(s) 163
BsaHI GRCGYC 2 cut(s) 93, 317
BsaJI CCNNGG 1 cut(s) 489
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse118I RCCGGY 1 cut(s) 460
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 536
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 26
BseRI GAGGAG 2 cut(s) 212, 514
BseXI GCAGC 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 3 cut(s) 116, 301, 464
BshNI GGYRCC 1 cut(s) 397
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 2 cut(s) 48, 124
BsiSI CCGG 3 cut(s) 117, 163, 461
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 3 cut(s) 116, 301, 464
Bsp119I TTCGAA 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 48, 124
Bsp143I GATC 4 cut(s) 165, 210, 417, 508
BspACI CCGC 2 cut(s) 28, 52
BspANI GGCC 3 cut(s) 116, 301, 464
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 3 cut(s) 351, 399, 459
BspMI ACCTGC 2 cut(s) 68, 361
BspPI GGATC 2 cut(s) 173, 412
BspT104I TTCGAA 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 397
BsrFI RCCGGY 1 cut(s) 460
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 4 cut(s) 165, 210, 417, 508
BssNI GRCGYC 2 cut(s) 93, 317
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 135
BstACI GRCGYC 2 cut(s) 93, 317
BstAPI GCANNNNNTGC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 188
BstDEI CTNAG 4 cut(s) 12, 103, 393, 495
BstF5I GGATG 1 cut(s) 536
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 4 cut(s) 168, 213, 420, 511
BstMBI GATC 4 cut(s) 165, 210, 417, 508
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 4 cut(s) 43, 60, 74, 443
BstSCI CCNGG 1 cut(s) 161
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BsuRI GGCC 3 cut(s) 116, 301, 464
BtsCI GGATG 1 cut(s) 536
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 1 cut(s) 511
BveI ACCTGC 2 cut(s) 68, 361
CciI TCATGA 1 cut(s) 273
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 114, 458
CseI GACGC 2 cut(s) 101, 306
CviAII CATG 5 cut(s) 169, 227, 266, 274, 561
DdeI CTNAG 4 cut(s) 12, 103, 393, 495
DpnI GATC 4 cut(s) 167, 212, 419, 510
DpnII GATC 4 cut(s) 165, 210, 417, 508
DraIII CACNNNGTG 1 cut(s) 452
EaeI YGGCCR 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 489
Eco147I AGGCCT 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 122
EcoICRI GAGCTC 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 5 cut(s) 172, 230, 269, 277, 564
FaqI GGGAC 1 cut(s) 471
FatI CATG 5 cut(s) 168, 226, 265, 273, 560
FauNDI CATATG 2 cut(s) 411, 524
Fnu4HI GCNGC 2 cut(s) 52, 339
FokI GGATG 1 cut(s) 543
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 2 cut(s) 52, 339
FspBI CTAG 1 cut(s) 449
GlaI GCGC 1 cut(s) 255
GluI GCNGC 2 cut(s) 52, 339
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 3 cut(s) 116, 301, 464
HapII CCGG 3 cut(s) 117, 163, 461
HgaI GACGC 2 cut(s) 101, 306
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 2 cut(s) 93, 317
Hin1II CATG 5 cut(s) 172, 230, 269, 277, 564
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HinfI GANTC 3 cut(s) 158, 270, 277
HpaII CCGG 3 cut(s) 117, 163, 461
Hpy188I TCNGA 4 cut(s) 43, 147, 322, 372
Hpy188III TCNNGA 6 cut(s) 14, 89, 274, 281, 310, 331
Hpy99I CGWCG 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 4 cut(s) 4, 409, 545, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 60, 74, 443
HpyF3I CTNAG 4 cut(s) 12, 103, 393, 495
HpySE526I ACGT 1 cut(s) 582
Hsp92I GRCGYC 2 cut(s) 93, 317
Hsp92II CATG 5 cut(s) 172, 230, 269, 277, 564
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 4 cut(s) 165, 210, 417, 508
LmnI GCTCC 3 cut(s) 119, 501, 569
Lsp1109I GCAGC 1 cut(s) 325
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 1 cut(s) 385
MalI GATC 4 cut(s) 167, 212, 419, 510
MboI GATC 4 cut(s) 165, 210, 417, 508
MflI RGATCY 1 cut(s) 417
MhlI GDGCHC 2 cut(s) 48, 124
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 3 cut(s) 300, 547, 603
MnlI CCTC 8 cut(s) 41, 96, 190, 193, 286, 327, 481, 492
MroXI GAANNNNTTC 1 cut(s) 484
MspI CCGG 3 cut(s) 117, 163, 461
MspR9I CCNGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 4 cut(s) 43, 60, 74, 443
NciI CCSGG 1 cut(s) 163
NdeI CATATG 2 cut(s) 411, 524
NdeII GATC 4 cut(s) 165, 210, 417, 508
NlaIII CATG 5 cut(s) 172, 230, 269, 277, 564
NlaIV GGNNCC 3 cut(s) 351, 399, 459
NmeAIII GCCGAG 2 cut(s) 121, 367
NmuCI GTSAC 1 cut(s) 385
NspV TTCGAA 1 cut(s) 188
PagI TCATGA 1 cut(s) 273
PceI AGGCCT 1 cut(s) 301
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 2 cut(s) 270, 277
PfoI TCCNGGA 1 cut(s) 161
PkrI GCNGC 2 cut(s) 53, 340
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp124BI GAGCTC 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 351, 399, 459
PspPI GGNCC 2 cut(s) 114, 458
PsuI RGATCY 1 cut(s) 417
SacI GAGCTC 1 cut(s) 124
SatI GCNGC 2 cut(s) 52, 339
Sau3AI GATC 4 cut(s) 165, 210, 417, 508
Sau96I GGNCC 2 cut(s) 114, 458
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 2 cut(s) 48, 124
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 1 cut(s) 458
SmlI CTYRAG 3 cut(s) 87, 472, 512
SmoI CTYRAG 3 cut(s) 87, 472, 512
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 2 cut(s) 28, 52
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 1 cut(s) 124
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 1 cut(s) 135
TaiI ACGT 1 cut(s) 585
TaqI TCGA 4 cut(s) 24, 129, 188, 309
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 2 cut(s) 270, 277
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 3 cut(s) 243, 262, 290
TspRI CASTG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.