Rorug04G0427000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011151
Physical Location & Seq
Reverse (-)
58565290 .. 58567170
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0427000.1

Sequence Viewer

Length: 1104 bp
ATGGCCGCAAATAGAACCTTGATCGGCGAACAGGGTTATGATCGAGCTGAAGAAGTGAAGCAATTCGACGAGTCCAAGCTCGGAGTCAAAGGCCTCGTCGACTCGGGTCTCTCCTCCATCCCCCGAATCTTTATCCACCCGCCCGAAGTATTACAGCAATTCCAGCCCACGACCCGACCCAACGCCCAACCAATCCCCACCATCGACCTCTCCGACCTCCACTCCGATCGCCGTTCCTCCATCGTCGACCAAGTCAGCGGCGCGTGCCGCAACTTCGGTTTCTTCCAGATCACTAACCACGGCATACCGTCGGTGGTTCTCGACCGTACGATCGCCGCCGTCAAGGCCTTCCACGAGCAGCCTACGGAAGTCAAAGCGCGGTTCTACCGTAGAGAGATGATAGGCCATGGCGTAACGTATCTATCCAACGTTGACCTGTACACCTCCAAAGCCGCCAGCTGGCGCGACACGCTTCAGGTGAGGCTCGGCCCTTTGCTGGAGGCCGCCGACGACGTTCCGAAGATTTGTAGGGACGAAATAGTTGAGTGGGACCGGGAGATCCGACGGCTGGGAGAGGAGCTTCTGGGGCTGGTGAGTGAAGGGCTGGGATTGCGCGCGGAGAAGCTGAAGGAGATGACGTGTTTGGGTGGGAGGGCGATGGTGGGCCACTACTACCCGCCCTGCCCGCAACCTGATCTAACGGTTGGGCTTACTTCACATAGCGATCCGGGAGTCTTGACCGTGCTGCTTCAAGATCATATTGGTGGGTTGCAGGTTAAGCACGATGGTGCTTGGGTGGATGTGAAACCTGTCCCCGGTGCTCTTGTGATCAACGTTGGTGATTTACTCCAGATTATTTCAAATGAGGAATACAAAAGTGTCGATCACCGGGTGTTGGCAAATCCACACCACGAGCCACGTGTTTCGATGGCCGTGTTTTTAAACCCGAGCAACAGAGAGGGGGTGTTTGGTCCGTTGCCAGAGTTCATCTCACCGGAGAAGCCCGCTCTCTACAAAGAGTTTAAGTTTTCGGAATTCCTATTGAGGTTCTTCAAGAAAGAATTGAATGGCAAATCTTTGGTAAATTACTATAAGCTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

367

Amino Acids

41.08

Weight (kDa)

6.2

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 161 2.1e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 219 - 316 7e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11120 AT1G11120
fragaria_vesca FvH4_3g04540 FvH4_6g10290
malus_domestica MD05G1181400.v1.1 MD05G1322600.v1.1 MD14G1018300.v1.1
prunus_persica Prupe.4G040100_v2.0.a1
pyrus_communis pycom05g29680 pycom10g25330
rosa_chinensis RchiOBHm_Chr5g0007461
rosa_laevigata RLG00000031458
rosa_multiflora Rmu_ssc0000182.1_g000001
rosa_roxburghii Rroxscaffold_1G00068930
rosa_rugosa Rorug04G0427000
rosa_samantha Rh5AG055400 Rh5BG053000 Rh5CG062800 Rh5DG052000
rosa_wichuraiana Rw5G005470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 763
AccBSI CCGCTC 1 cut(s) 1007
AccI GTMKAC 2 cut(s) 99, 246
AccII CGCG 5 cut(s) 263, 379, 465, 615, 617
AclI AACGTT 2 cut(s) 429, 834
AclWI GGATC 2 cut(s) 553, 719
AcoI YGGCCR 2 cut(s) 3, 930
AcsI RAATTY 1 cut(s) 1034
AcuI CTGAAG 3 cut(s) 69, 458, 647
AcvI CACGTG 1 cut(s) 920
AdeI CACNNNGTG 1 cut(s) 892
AfaI GTAC 2 cut(s) 328, 440
AfiI CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
AflIII ACRYGT 2 cut(s) 638, 919
AgsI TTSAA 4 cut(s) 752, 861, 1054, 1066
AjiI CACGTC 1 cut(s) 639
AleI CACNNNNGTG 1 cut(s) 786
AluBI AGCT 6 cut(s) 47, 79, 459, 580, 625, 1096
AluI AGCT 6 cut(s) 47, 79, 459, 580, 625, 1096
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 1 cut(s) 113
AlwI GGATC 2 cut(s) 553, 719
Ama87I CYCGRG 2 cut(s) 103, 946
AoxI GGCC 8 cut(s) 3, 91, 345, 403, 487, 501, 664, 930
ApeKI GCWGC 3 cut(s) 358, 745, 1096
ApoI RAATTY 1 cut(s) 1034
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 5 cut(s) 263, 379, 465, 615, 617
AspS9I GGNCC 4 cut(s) 488, 550, 664, 971
AsuC2I CCSGG 4 cut(s) 554, 729, 816, 890
AsuHPI GGTGA 5 cut(s) 490, 604, 851, 878, 984
AvaI CYCGRG 2 cut(s) 103, 946
AvaII GGWCC 2 cut(s) 550, 971
BauI CACGAG 2 cut(s) 353, 911
BbrPI CACGTG 1 cut(s) 920
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 3 cut(s) 370, 732, 1083
BccI CCATC 6 cut(s) 125, 209, 248, 652, 779, 922
BceAI ACGGC 5 cut(s) 216, 316, 323, 581, 917
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 4 cut(s) 554, 729, 816, 890
BcoDI GTCTC 1 cut(s) 113
BfmI CTRYAG 1 cut(s) 1097
BfuAI ACCTGC 1 cut(s) 763
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 9 cut(s) 6, 259, 268, 336, 359, 453, 504, 746, 1097
BlsI GCNGC 9 cut(s) 7, 260, 269, 337, 360, 454, 505, 747, 1098
Bme1390I CCNGG 4 cut(s) 554, 729, 816, 890
Bme18I GGWCC 2 cut(s) 550, 971
BmeT110I CYCGRG 2 cut(s) 103, 946
BmgBI CACGTC 1 cut(s) 639
BmgT120I GGNCC 4 cut(s) 488, 550, 664, 971
BmiI GGNNCC 1 cut(s) 551
BmrFI CCNGG 4 cut(s) 554, 729, 816, 890
BoxI GACNNNNGTC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 974, 1006
BpmI CTGGAG 2 cut(s) 518, 833
BpuMI CCSGG 4 cut(s) 554, 729, 816, 890
BsaAI YACGTR 1 cut(s) 920
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 298, 406, 814
BsaWI WCCGGW 1 cut(s) 994
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 623, 653
Bsc4I CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
BseDI CCNNGG 3 cut(s) 298, 406, 814
BseGI GGATG 2 cut(s) 117, 805
BseLI CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
BsePI GCGCGC 1 cut(s) 613
BseRI GAGGAG 2 cut(s) 103, 590
BseXI GCAGC 3 cut(s) 370, 732, 1083
BseYI CCCAGC 2 cut(s) 568, 604
Bsh1236I CGCG 5 cut(s) 263, 379, 465, 615, 617
Bsh1285I CGRYCG 3 cut(s) 229, 325, 333
BshFI GGCC 8 cut(s) 5, 93, 347, 405, 489, 503, 666, 932
BsiEI CGRYCG 3 cut(s) 229, 325, 333
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 2 cut(s) 103, 946
BsiSI CCGG 5 cut(s) 553, 728, 816, 889, 995
BsiWI CGTACG 1 cut(s) 326
BslFI GGGAC 3 cut(s) 545, 563, 797
BslI CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
BsmAI GTCTC 1 cut(s) 113
BsmFI GGGAC 3 cut(s) 545, 563, 797
BsnI GGCC 8 cut(s) 5, 93, 347, 405, 489, 503, 666, 932
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 2 cut(s) 103, 946
Bsp1286I GDGCHC 1 cut(s) 823
Bsp1407I TGTACA 1 cut(s) 438
Bsp19I CCATGG 1 cut(s) 406
BspANI GGCC 8 cut(s) 5, 93, 347, 405, 489, 503, 666, 932
BspFNI CGCG 5 cut(s) 263, 379, 465, 615, 617
BspLI GGNNCC 1 cut(s) 551
BspMAI CTGCAG 1 cut(s) 1101
BspMI ACCTGC 1 cut(s) 763
BspPI GGATC 2 cut(s) 553, 719
BspTNI GGTCTC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 1007
BsrGI TGTACA 1 cut(s) 438
BssECI CCNNGG 3 cut(s) 298, 406, 814
BssHII GCGCGC 1 cut(s) 613
BssSI CACGAG 2 cut(s) 353, 911
BssT1I CCWWGG 1 cut(s) 406
Bst2BI CACGAG 2 cut(s) 353, 911
Bst4CI ACNGT 5 cut(s) 309, 326, 389, 703, 742
BstAUI TGTACA 1 cut(s) 438
BstBAI YACGTR 1 cut(s) 920
BstC8I GCNNGC 6 cut(s) 265, 457, 461, 615, 686, 1005
BstDSI CCRYGG 2 cut(s) 298, 406
BstF5I GGATG 2 cut(s) 117, 805
BstFNI CGCG 5 cut(s) 263, 379, 465, 615, 617
BstHHI GCGC 5 cut(s) 263, 379, 465, 615, 617
BstMAI GTCTC 1 cut(s) 113
BstMCI CGRYCG 3 cut(s) 229, 325, 333
BstMWI GCNNNNNNNGC 9 cut(s) 163, 264, 267, 344, 469, 586, 610, 685, 778
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 4 cut(s) 552, 727, 814, 888
BstSFI CTRYAG 1 cut(s) 1097
BstUI CGCG 5 cut(s) 263, 379, 465, 615, 617
BstV1I GCAGC 3 cut(s) 370, 732, 1083
BstX2I RGATCY 1 cut(s) 558
BstYI RGATCY 1 cut(s) 558
BsuRI GGCC 8 cut(s) 5, 93, 347, 405, 489, 503, 666, 932
BtgI CCRYGG 2 cut(s) 298, 406
BtgZI GCGATG 1 cut(s) 671
BtrI CACGTC 1 cut(s) 639
BtsCI GGATG 2 cut(s) 117, 805
BveI ACCTGC 1 cut(s) 763
Cac8I GCNNGC 6 cut(s) 265, 457, 461, 615, 686, 1005
CfoI GCGC 5 cut(s) 263, 379, 465, 615, 617
Cfr13I GGNCC 4 cut(s) 488, 550, 664, 971
Csp6I GTAC 2 cut(s) 327, 439
CviAII CATG 1 cut(s) 407
CviQI GTAC 2 cut(s) 327, 439
DraI TTTAAA 1 cut(s) 942
DraIII CACNNNGTG 1 cut(s) 892
EaeI YGGCCR 2 cut(s) 3, 930
Eco130I CCWWGG 1 cut(s) 406
Eco147I AGGCCT 2 cut(s) 93, 347
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 2 cut(s) 550, 971
Eco57I CTGAAG 3 cut(s) 69, 458, 647
Eco72I CACGTG 1 cut(s) 920
Eco88I CYCGRG 2 cut(s) 103, 946
EcoRI GAATTC 1 cut(s) 1034
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 1 cut(s) 410
FaiI YATR 6 cut(s) 39, 305, 408, 720, 759, 1092
FaqI GGGAC 3 cut(s) 545, 563, 797
FatI CATG 1 cut(s) 406
FauI CCCGC 4 cut(s) 147, 684, 693, 1012
FbaI TGATCA 1 cut(s) 828
FblI GTMKAC 2 cut(s) 99, 246
Fnu4HI GCNGC 9 cut(s) 6, 259, 268, 336, 359, 453, 504, 746, 1097
FokI GGATG 2 cut(s) 104, 812
Fsp4HI GCNGC 9 cut(s) 6, 259, 268, 336, 359, 453, 504, 746, 1097
GlaI GCGC 5 cut(s) 262, 378, 464, 614, 616
GluI GCNGC 9 cut(s) 6, 259, 268, 336, 359, 453, 504, 746, 1097
GsaI CCCAGC 2 cut(s) 572, 608
GsuI CTGGAG 2 cut(s) 518, 833
HaeIII GGCC 8 cut(s) 5, 93, 347, 405, 489, 503, 666, 932
HapII CCGG 5 cut(s) 553, 728, 816, 889, 995
HhaI GCGC 5 cut(s) 263, 379, 465, 615, 617
Hin1II CATG 1 cut(s) 410
Hin6I GCGC 5 cut(s) 261, 377, 463, 613, 615
HinP1I GCGC 5 cut(s) 261, 377, 463, 613, 615
HincII GTYRAC 3 cut(s) 100, 247, 433
HindII GTYRAC 3 cut(s) 100, 247, 433
HinfI GANTC 5 cut(s) 71, 84, 101, 126, 732
HpaII CCGG 5 cut(s) 553, 728, 816, 889, 995
HphI GGTGA 5 cut(s) 490, 604, 851, 878, 984
Hpy166II GTNNAC 4 cut(s) 100, 247, 433, 441
Hpy188I TCNGA 6 cut(s) 83, 214, 226, 519, 563, 1033
Hpy188III TCNNGA 6 cut(s) 286, 320, 736, 752, 850, 1054
Hpy8I GTNNAC 4 cut(s) 100, 247, 433, 441
Hpy99I CGWCG 7 cut(s) 71, 101, 248, 313, 512, 515, 567
HpyAV CCTTC 3 cut(s) 358, 593, 622
HpyCH4III ACNGT 5 cut(s) 309, 326, 389, 703, 742
HpyCH4IV ACGT 6 cut(s) 416, 429, 513, 638, 834, 919
HpyCH4V TGCA 2 cut(s) 772, 1099
HpyF10VI GCNNNNNNNGC 9 cut(s) 163, 264, 267, 344, 469, 586, 610, 685, 778
HpySE526I ACGT 6 cut(s) 416, 429, 513, 638, 834, 919
Hsp92II CATG 1 cut(s) 410
HspAI GCGC 5 cut(s) 261, 377, 463, 613, 615
Ksp22I TGATCA 1 cut(s) 828
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 3 cut(s) 370, 732, 1083
MaeII ACGT 6 cut(s) 416, 429, 513, 638, 834, 919
MaeIII GTNAC 1 cut(s) 412
MbiI CCGCTC 1 cut(s) 1007
MboII GAAGA 4 cut(s) 62, 274, 532, 1042
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 823
MluCI AATT 5 cut(s) 62, 158, 1034, 1061, 1084
MlyI GAGTC 4 cut(s) 80, 93, 95, 741
MmeI TCCRAC 3 cut(s) 237, 450, 586
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 3 cut(s) 777, 941, 1023
MslI CAYNNNNRTG 2 cut(s) 762, 786
MspA1I CMGCKG 2 cut(s) 258, 459
MspI CCGG 5 cut(s) 553, 728, 816, 889, 995
MspR9I CCNGG 4 cut(s) 554, 729, 816, 890
MvnI CGCG 5 cut(s) 263, 379, 465, 615, 617
MwoI GCNNNNNNNGC 9 cut(s) 163, 264, 267, 344, 469, 586, 610, 685, 778
NciI CCSGG 4 cut(s) 554, 729, 816, 890
NcoI CCATGG 1 cut(s) 406
NlaIII CATG 1 cut(s) 410
NlaIV GGNNCC 1 cut(s) 551
NmeAIII GCCGAG 1 cut(s) 465
OliI CACNNNNGTG 1 cut(s) 786
PauI GCGCGC 1 cut(s) 613
PceI AGGCCT 2 cut(s) 93, 347
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 126
Pfl23II CGTACG 1 cut(s) 326
PflFI GACNNNGTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 727
PkrI GCNGC 9 cut(s) 7, 260, 269, 337, 360, 454, 505, 747, 1098
Ple19I CGATCG 2 cut(s) 229, 333
PleI GAGTC 4 cut(s) 79, 92, 95, 740
PmaCI CACGTG 1 cut(s) 920
PmlI CACGTG 1 cut(s) 920
PpsI GAGTC 4 cut(s) 79, 92, 95, 740
Ppu21I YACGTR 1 cut(s) 920
PshAI GACNNNNGTC 1 cut(s) 105
Psp1406I AACGTT 2 cut(s) 429, 834
PspCI CACGTG 1 cut(s) 920
PspFI CCCAGC 2 cut(s) 568, 604
PspLI CGTACG 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 551
PspPI GGNCC 4 cut(s) 488, 550, 664, 971
PstI CTGCAG 1 cut(s) 1101
PsuI RGATCY 1 cut(s) 558
PsyI GACNNNGTC 1 cut(s) 251
PteI GCGCGC 1 cut(s) 613
PvuI CGATCG 2 cut(s) 229, 333
PvuII CAGCTG 1 cut(s) 459
RsaI GTAC 2 cut(s) 328, 440
RsaNI GTAC 2 cut(s) 327, 439
RseI CAYNNNNRTG 2 cut(s) 762, 786
SalI GTCGAC 2 cut(s) 98, 245
SaqAI TTAA 3 cut(s) 777, 941, 1023
SatI GCNGC 9 cut(s) 6, 259, 268, 336, 359, 453, 504, 746, 1097
Sau96I GGNCC 4 cut(s) 488, 550, 664, 971
SchI GAGTC 4 cut(s) 80, 93, 95, 741
ScrFI CCNGG 4 cut(s) 554, 729, 816, 890
SduI GDGCHC 1 cut(s) 823
SfcI CTRYAG 1 cut(s) 1097
SinI GGWCC 2 cut(s) 550, 971
SmiMI CAYNNNNRTG 2 cut(s) 762, 786
Sse9I AATT 5 cut(s) 62, 158, 1034, 1061, 1084
SseBI AGGCCT 2 cut(s) 93, 347
StuI AGGCCT 2 cut(s) 93, 347
StyD4I CCNGG 4 cut(s) 552, 727, 814, 888
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 5 cut(s) 309, 326, 389, 703, 742
TaiI ACGT 6 cut(s) 419, 432, 516, 641, 837, 922
TaqI TCGA 8 cut(s) 43, 66, 99, 204, 246, 321, 882, 926
TasI AATT 5 cut(s) 62, 158, 1034, 1061, 1084
TatI WGTACW 1 cut(s) 438
TauI GCSGC 6 cut(s) 8, 261, 270, 338, 455, 506
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 3 cut(s) 777, 941, 1023
Tru9I TTAA 3 cut(s) 777, 941, 1023
TseI GCWGC 3 cut(s) 358, 745, 1096
TspDTI ATGAA 1 cut(s) 976
TspGWI ACGGA 2 cut(s) 380, 963
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 2 cut(s) 550, 971
XapI RAATTY 1 cut(s) 1034
XmiI GTMKAC 2 cut(s) 99, 246
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.