Rorug05G0103600

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005444
Physical Location & Seq
Forward (+)
9122700 .. 9127395
4696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0103600.1

Sequence Viewer

Length: 3612 bp
ATGGCTTCTTCTTCTTCTTCTCCTGCGCATGCCTTCAAGTATGATGTCTTTCTTAGTTTCAGAGGTGAGGACACCCGTAACACTTTTACCAGTCATCTTTATGCGGCTTTAGATGGGAAGAAAATCAAAACCTACATAGATAATTATGACCTTGTGAGAGGAGAGGAAATTGCACCTGCGCTTCTTGAAGCAATAGAGGAATCAAAGCTTTCTGTTGTCATTTTGTCACAAGACTACGCTTCTTCTACATGGTGCTTGGACGAACTCGTGCAGATTTTGGAATGCAAGGAAAGACATGGGCAGATTGTTATACCCATTTTTTACAGAATAGATCCTTCACATGTACGCCACCAGAGGGAGAGTTATGCAGCTGCATTTGTTCGACATGAAGAACGCTTCAAGGGCAAGGTGCTCAAGTGGAGGGATGCTTTGACAAAAGCATCCAAACTAGCAGGGTTTGATTCACGCAATATCAGCCTTGAATCCGAATTAGTGGAGTTAGTCGTCAAAGATATTGTGACGAAGTTGAGCCGCGCATGCTCAAGCGATTTAAAGGGTCTGGTTGGAATTGAAAGTCGCATCCAGCAACTCGAGTCATTGTTATGCATTGATGTAGAAGATGTTCGTGTTCGCACTGTTGGTATTTGGGGCATGGGTGGTATAGGCAAGACCACCCTTGCTGATGCTGTATTCAACCGACTCTCATCTCAATTTGAAGCTTCATGTTTTCTGTCAAATGTTAGGGAAAAATCAGAAAAACATGGACCATACAATTTGCGAAATGAACTTCTTTGCAATTTATTGAAGGATGAAAGTCCAAGGATTTGCACTCCATCAATAGCATCAACTTTTATCAAAGAGAGGCTCTCTCGTGCAAAGGTTCTCGTTGTTCTTGATGATGTAAATGATGTAACCCAATTAGAAGTCTTGGTTGGTGATCAGGTTCAATTTGGACCAGGAAGTAGAATCATAATAACAACTAGAGATATGCATCAACTTAGAGAGATGCAACTACTGCAGAAAGGATCAGACCATGATGTTAAAATATACGAGGCACAGGAACTAGATCATGACGACGCACTTCAGCTCTTCTACTTGAATGATTCCGAAGAAATCAGCTGCAAAGCTGATCATTCTGAATTAGTAAGAATGGTGGTACATTATGCTGCGGGAATCCCCTTGGCCCTTAAAATTTGGGGTTCCTTATTTCGTCGATGCAAGAGCGCACAAGAACGGGCAACTTTCTTGGAAAAATTGAAAAAGTTCCCTGAAAAGAAACTTCAAAATGTATATAGGATAAGTTATGATGCATTAGGAGAAAATGAGAGGGAGATATTTCTTTACATCGCCTGTTTTCACAAAGGACATGAAATATGCAGTGCAATAGAACAATTACATGCATGTGGTTTATTTGCGGATAGTGGAATCGAAGTTCTCGTTGATATGTCTCTGATATCAATCGAAGATGATAGGCGGACCCGCGTAGAAGAAAAAACTCGTTACCTGTGGATGCATGATGTGATACAAGAAATGGGATGGGCAATTCAGTATGAACAAGATCCTGCGAAGCCTGGAAAGCGCCGTAGGCTGTGTACCGCGGAGGACATCTGTCATGTGTTGAAAAAGAGTAAGGGAACAGAAAAAGTTCAAAGCATATCCCTAAACCTCTCTCAGATTACAGAGCTAAACTTGACTCCTCAGGCCTTCAAAAAGATGTGCAATCTAAAATTTCTAAAGTTCTATGGTGAAGATCGCAGGGGATACTACTACCATCGTTGGAAGGACTTCAATGTGCACTTTAATCTTCAAGATCTTGAATATCTTCCCGATTCCCTTTTACATCTCAGCTGGCCTGCATACACTTTGAAATATTTTCCATCAACATTTTCTGCAGAGAATCTTACTGTGCTTTCTATGCCTGGTAGCCGACTCCAGAGACTTTGGGATGAAGGCCAGAAGCCTAGGTACTTAAAACACATCGATCTCAGTCACTCCCAGCAGCTGGTTGAAGTTCCAGATCTCTCTATGAGCGTAAACATTGAGAGGATAAACCTTGAAGGCTGTGAAGGGCTGGTTGAACTTCCTTCCTATTTTGAAAATCTTCACAAGCTTACTTCGTTGAATGTGAGCTGGTGCCGGAATCTTAGTGTTCTCTCAGATATGCCATGCAATGTGGTTTCGTTGATGTTAGCTGGGACTGCAATAGAAGACCTGCCTTCATCAATTTGGTCTCAAGAAAAACTTGTTGAATTGAATCTTCATTGCTGCTGTTGGATTAAGAAACTTCCAAACAGCATTTGGAAGTTGTATTTCCTCACATCTCTTGACTTGTCTTGTACAAGTATAGAAGGATTGCCCCCGTCAATCGAGTGTCTCCCTGGGTTAAAAATTCTTGATCTGCAAAATTGCAAAAGGTTTGTCAGTCTCCCAACCAGCATTTGTAAGTTGAAATCTCTCGAGGACTTGCTTCTTAATGGATGCTCTAGCTTCGAAAACTTCCCTGAGATCTTGGAGCCTATGGAACGTCTGAAGTCTCTTTGGTTAGGAGGAACGAAATTCAAAGAGCTACCCTGTTTGATTGAAAACGTATTTGTGCGGAAGGAATTAGTGTTACCTGGGTGTAGAAGCCTTGAGTCTGTCCCAAACAGCATCTTACCTCCGTCGTCGGTCATTTTATGGTCTTCGATGAGTAGTCTGTACCTCGGTTACTGCACAATGTTAGAAGAGATCCCTGATTGCATCTTTAGCCTAAACAGGTTATCTGAATTAAATCTGAGTGGAACCATGATTAGGAGCATACCTTCAACCATCAATCAAGCATCTCGGCTGAGGTCATTATGTCTCCAGGATTGCAAGCGCCTTGAATCTTTACCCAAGCTCCCATATTTTCTACAATTGATAAATGCAAGTGGCTGCAAGAGATTGAAGACAGTGTCTGGGTTCACGACTGCACTCACAGAAGGTTCGGATCAAAGTGATTCTTTCGTGTTGCATATATTTAATAATTGCGTAAGCTTGGATGAGAATGCGAGGAGGAACATAATGGATGATGCACATCTTAGAAATATGGCAACAGCTTATTATCCATATGTACAGATATTTACGTACGAAGTCAGATGTCGGGGAATTGAAATTCCAAAATGGGTGGGCAATCAAATGGAGGGATCTTCGATAGATATCACGCTTCCTCTACATTGGTCTGAAGATCCAAACTTTTTGGGTTTAGCTTTCTGTCTTGTTGTTGACTTCCGTAAAGCACCAAATGCGACATGTGAGGGTGAATCCATTCTCAAAACCGAAAATGGTGAAAGCCGTACCTTTAAGTTTAGCAATTATATGAACTATTGTCCCCCTAATGATGCCGACAACCCAGATCACCTGGTGGTGGTGTGGTACGATTCCCTTCAGAAGGTAAGGTCCACTCCTTATACCAATGCCACTCAAGCCTCTTTTCATTTCCATGAACGGACTGAGATGAATCATCATGTAGAGAAGTTCAAGTATAGGGTGAAAAGGTGTGGGGTGGGGTTTGTGTATGCTCAAGGCCAAGACCGAAATGCTGTGAAGTGGCAAGTCATGGATGATCAATATGTTGAAGCTTCCCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-MYND

Protein Analysis

1203

Amino Acids

137.46

Weight (kDa)

6.15

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 190 1.4e-58 TIR domain
TIR_2 PF13676 16 - 113 1.1e-15 TIR domain
NB-ARC PF00931 209 - 364 6.6e-15 NB-ARC domain
WHD_ROQ1 PF23282 440 - 518 1.5e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 757 - 848 3.9e-12 Leucine-rich repeat region
C-JID PF20160 1041 - 1181 8.3e-13 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 184
Acc16I TGCGCA 1 cut(s) 27
Acc36I ACCTGC 2 cut(s) 184, 2220
AccB1I GGYRCC 1 cut(s) 2133
AccB7I CCANNNNNTGG 1 cut(s) 2002
AccII CGCG 3 cut(s) 534, 1482, 1598
AciI CCGC 9 cut(s) 104, 532, 1169, 1415, 1474, 1480, 1596, 1598, 2596
AclWI GGATC 7 cut(s) 326, 1033, 1553, 2722, 2976, 3172, 3200
AcsI RAATTY 5 cut(s) 1191, 1727, 2388, 2555, 3132
AcuI CTGAAG 4 cut(s) 1067, 2549, 3222, 3389
AdeI CACNNNGTG 1 cut(s) 3382
AfiI CCNNNNNNNGG 5 cut(s) 355, 2002, 2790, 3385, 3409
AflIII ACRYGT 2 cut(s) 340, 3269
AjnI CCWGG 7 cut(s) 955, 1570, 1918, 2377, 2614, 2844, 3378
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AloI GAACNNNNNNTCC 2 cut(s) 1416, 1448
Alw21I GWGCWC 2 cut(s) 414, 1797
Alw26I GTCTC 7 cut(s) 1452, 1930, 2235, 2378, 2429, 2538, 2846
Alw44I GTGCAC 1 cut(s) 1793
AlwI GGATC 7 cut(s) 326, 1033, 1553, 2722, 2976, 3172, 3200
AlwNI CAGNNNCTG 2 cut(s) 2002, 2936
Ama87I CYCGRG 2 cut(s) 590, 2456
AoxI GGCC 5 cut(s) 1182, 1701, 1850, 1951, 3544
ApaLI GTGCAC 1 cut(s) 1793
ApeKI GCWGC 7 cut(s) 368, 371, 1119, 1166, 1999, 2265, 2913
ApoI RAATTY 5 cut(s) 1191, 1727, 2388, 2555, 3132
ArsI GACNNNNNNTTYG 2 cut(s) 2403, 2435
Asp700I GAANNNNTTC 7 cut(s) 621, 1262, 1644, 1784, 1821, 2100, 3285
AspA2I CCTAGG 1 cut(s) 1961
AspLEI GCGC 6 cut(s) 28, 181, 536, 1226, 1581, 2859
AspS9I GGNCC 5 cut(s) 764, 953, 1183, 1476, 3417
AsuHPI GGTGA 7 cut(s) 77, 947, 1757, 3290, 3317, 3368, 3520
AsuII TTCGAA 1 cut(s) 2490
AvaI CYCGRG 2 cut(s) 590, 2456
AvaII GGWCC 4 cut(s) 764, 953, 1476, 3417
AvrII CCTAGG 1 cut(s) 1961
AxyI CCTNAGG 1 cut(s) 1698
BaeGI GKGCMC 1 cut(s) 1797
BanI GGYRCC 1 cut(s) 2133
BarI GAAGNNNNNNTAC 2 cut(s) 1949, 1981
BauI CACGAG 2 cut(s) 266, 870
BbsI GAAGAC 3 cut(s) 2214, 2673, 2933
Bbv12I GWGCWC 2 cut(s) 414, 1797
BbvCI CCTCAGC 1 cut(s) 2828
BbvI GCAGC 7 cut(s) 358, 380, 1106, 1153, 2011, 2252, 2900
BccI CCATC 6 cut(s) 107, 841, 1530, 1779, 1885, 2816
BceAI ACGGC 2 cut(s) 1566, 3297
BciT130I CCWGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
BciVI GTATCC 1 cut(s) 1754
BclI TGATCA 3 cut(s) 937, 1129, 3583
BcoDI GTCTC 7 cut(s) 1452, 1930, 2235, 2378, 2429, 2538, 2846
BfaI CTAG 5 cut(s) 449, 981, 1064, 1962, 2484
BfmI CTRYAG 2 cut(s) 1016, 1890
BfoI RGCGCY 2 cut(s) 1582, 2860
BfuAI ACCTGC 2 cut(s) 184, 2220
BfuI GTATCC 1 cut(s) 1754
BglII AGATCT 3 cut(s) 1810, 2017, 2505
BisI GCNGC 9 cut(s) 105, 369, 372, 532, 1120, 1167, 2000, 2266, 2914
BlnI CCTAGG 1 cut(s) 1961
BlsI GCNGC 9 cut(s) 106, 370, 373, 533, 1121, 1168, 2001, 2267, 2915
Bme1390I CCNGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
Bme18I GGWCC 4 cut(s) 764, 953, 1476, 3417
BmeT110I CYCGRG 2 cut(s) 590, 2456
BmgT120I GGNCC 5 cut(s) 764, 953, 1183, 1476, 3417
BmiI GGNNCC 5 cut(s) 1201, 1478, 2135, 2514, 2782
BmrFI CCNGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
BoxI GACNNNNGTC 1 cut(s) 1608
BpiI GAAGAC 3 cut(s) 2214, 2673, 2933
BplI GAGNNNNNCTC 4 cut(s) 851, 883, 853, 885
BpmI CTGGAG 2 cut(s) 1916, 2828
Bpu10I CCTNAGC 1 cut(s) 2828
Bpu14I TTCGAA 1 cut(s) 2490
BpuEI CTTGAG 6 cut(s) 398, 526, 2217, 2651, 3426, 3525
Bsa29I ATCGAT 1 cut(s) 1980
BsaAI YACGTR 1 cut(s) 3105
BsaBI GATNNNNATC 4 cut(s) 990, 1457, 3054, 3176
BsaI GGTCTC 1 cut(s) 2235
BsaJI CCNNGG 8 cut(s) 818, 1179, 1596, 1961, 2377, 2378, 2615, 2701
BsaXI ACNNNNNCTCC 2 cut(s) 2862, 2892
Bsc4I CCNNNNNNNGG 5 cut(s) 355, 2002, 2790, 3385, 3409
Bse1I ACTGG 1 cut(s) 90
Bse21I CCTNAGG 1 cut(s) 1698
Bse3DI GCAATG 2 cut(s) 2176, 2260
Bse8I GATNNNNATC 4 cut(s) 990, 1457, 3054, 3176
BseBI CCWGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
BseCI ATCGAT 1 cut(s) 1980
BseDI CCNNGG 8 cut(s) 818, 1179, 1596, 1961, 2377, 2378, 2615, 2701
BseJI GATNNNNATC 4 cut(s) 990, 1457, 3054, 3176
BseLI CCNNNNNNNGG 5 cut(s) 355, 2002, 2790, 3385, 3409
BseMI GCAATG 2 cut(s) 2176, 2260
BseMII CTCAG 9 cut(s) 1685, 1712, 1858, 1999, 2169, 2493, 2765, 2819, 3462
BseNI ACTGG 1 cut(s) 90
BseRI GAGGAG 3 cut(s) 174, 1686, 3046
BseSI GKGCMC 1 cut(s) 1797
BseXI GCAGC 7 cut(s) 358, 380, 1106, 1153, 2011, 2252, 2900
BseYI CCCAGC 2 cut(s) 1995, 2192
BsgI GTGCAG 3 cut(s) 290, 2695, 2934
Bsh1236I CGCG 3 cut(s) 534, 1482, 1598
BshFI GGCC 5 cut(s) 1184, 1703, 1852, 1953, 3546
BshNI GGYRCC 1 cut(s) 2133
BshVI ATCGAT 1 cut(s) 1980
BsiHKAI GWGCWC 2 cut(s) 414, 1797
BsiHKCI CYCGRG 2 cut(s) 590, 2456
BsiSI CCGG 1 cut(s) 2137
BsiWI CGTACG 1 cut(s) 3105
BslFI GGGAC 3 cut(s) 2209, 2624, 3333
BslI CCNNNNNNNGG 5 cut(s) 355, 2002, 2790, 3385, 3409
BsmAI GTCTC 7 cut(s) 1452, 1930, 2235, 2378, 2429, 2538, 2846
BsmFI GGGAC 3 cut(s) 2209, 2624, 3333
BsmI GAATGC 2 cut(s) 287, 3031
BsnI GGCC 5 cut(s) 1184, 1703, 1852, 1953, 3546
Bso31I GGTCTC 1 cut(s) 2235
BsoBI CYCGRG 2 cut(s) 590, 2456
Bsp119I TTCGAA 1 cut(s) 2490
Bsp1286I GDGCHC 2 cut(s) 414, 1797
Bsp1407I TGTACA 2 cut(s) 2336, 3091
BspACI CCGC 9 cut(s) 104, 532, 1169, 1415, 1474, 1480, 1596, 1598, 2596
BspANI GGCC 5 cut(s) 1184, 1703, 1852, 1953, 3546
BspCNI CTCAG 9 cut(s) 1684, 1711, 1857, 1998, 2168, 2494, 2766, 2820, 3463
BspDI ATCGAT 1 cut(s) 1980
BspFNI CGCG 3 cut(s) 534, 1482, 1598
BspHI TCATGA 1 cut(s) 1069
BspLI GGNNCC 5 cut(s) 1201, 1478, 2135, 2514, 2782
BspMAI CTGCAG 2 cut(s) 1020, 1894
BspMI ACCTGC 2 cut(s) 184, 2220
BspPI GGATC 7 cut(s) 326, 1033, 1553, 2722, 2976, 3172, 3200
BspQI GCTCTTC 1 cut(s) 1094
BspT104I TTCGAA 1 cut(s) 2490
BspT107I GGYRCC 1 cut(s) 2133
BspTNI GGTCTC 1 cut(s) 2235
BsrDI GCAATG 2 cut(s) 2176, 2260
BsrGI TGTACA 2 cut(s) 2336, 3091
BsrI ACTGG 1 cut(s) 90
BssECI CCNNGG 8 cut(s) 818, 1179, 1596, 1961, 2377, 2378, 2615, 2701
BssSI CACGAG 2 cut(s) 266, 870
BssT1I CCWWGG 3 cut(s) 818, 1179, 1961
Bst2BI CACGAG 2 cut(s) 266, 870
Bst2UI CCWGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
Bst4CI ACNGT 3 cut(s) 637, 1906, 2932
Bst6I CTCTTC 2 cut(s) 1094, 2718
BstAPI GCANNNNNTGC 2 cut(s) 1015, 3263
BstAUI TGTACA 2 cut(s) 2336, 3091
BstBAI YACGTR 1 cut(s) 3105
BstBI TTCGAA 1 cut(s) 2490
BstC8I GCNNGC 5 cut(s) 30, 538, 1850, 1854, 2855
BstDSI CCRYGG 1 cut(s) 1596
BstENI CCTNNNNNAGG 1 cut(s) 3407
BstFNI CGCG 3 cut(s) 534, 1482, 1598
BstH2I RGCGCY 2 cut(s) 1582, 2860
BstHHI GCGC 6 cut(s) 28, 181, 536, 1226, 1581, 2859
BstMAI GTCTC 7 cut(s) 1452, 1930, 2235, 2378, 2429, 2538, 2846
BstNI CCWGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
BstNSI RCATGY 6 cut(s) 32, 344, 540, 1400, 1404, 3273
BstPAI GACNNNNGTC 1 cut(s) 1608
BstSCI CCNGG 7 cut(s) 955, 1570, 1918, 2377, 2614, 2844, 3378
BstSFI CTRYAG 2 cut(s) 1016, 1890
BstSLI GKGCMC 1 cut(s) 1797
BstSNI TACGTA 1 cut(s) 3105
BstUI CGCG 3 cut(s) 534, 1482, 1598
BstV1I GCAGC 7 cut(s) 358, 380, 1106, 1153, 2011, 2252, 2900
BstV2I GAAGAC 3 cut(s) 2214, 2673, 2933
BstX2I RGATCY 8 cut(s) 331, 1558, 1810, 2017, 2505, 2727, 3164, 3205
BstYI RGATCY 8 cut(s) 331, 1558, 1810, 2017, 2505, 2727, 3164, 3205
Bsu15I ATCGAT 1 cut(s) 1980
Bsu36I CCTNAGG 1 cut(s) 1698
BsuI GTATCC 1 cut(s) 1754
BsuRI GGCC 5 cut(s) 1184, 1703, 1852, 1953, 3546
BsuTUI ATCGAT 1 cut(s) 1980
BtgI CCRYGG 1 cut(s) 1596
BtgZI GCGATG 1 cut(s) 1330
BtsI GCAGTG 1 cut(s) 1384
BtsIMutI CAGTG 3 cut(s) 633, 1384, 2937
BveI ACCTGC 2 cut(s) 184, 2220
Cac8I GCNNGC 5 cut(s) 30, 538, 1850, 1854, 2855
CaiI CAGNNNCTG 2 cut(s) 2002, 2936
CciI TCATGA 1 cut(s) 1069
CfoI GCGC 6 cut(s) 28, 181, 536, 1226, 1581, 2859
Cfr13I GGNCC 5 cut(s) 764, 953, 1183, 1476, 3417
Cfr42I CCGCGG 1 cut(s) 1599
ClaI ATCGAT 1 cut(s) 1980
CseI GACGC 1 cut(s) 1085
CsiI ACCWGGT 1 cut(s) 3378
CspCI CAANNNNNGTGG 2 cut(s) 3126, 3161
DraI TTTAAA 1 cut(s) 552
DraIII CACNNNGTG 1 cut(s) 3382
Eam1104I CTCTTC 2 cut(s) 1094, 2718
EarI CTCTTC 2 cut(s) 1094, 2718
EciI GGCGGA 1 cut(s) 1489
Eco105I TACGTA 1 cut(s) 3105
Eco130I CCWWGG 3 cut(s) 818, 1179, 1961
Eco147I AGGCCT 1 cut(s) 1703
Eco31I GGTCTC 1 cut(s) 2235
Eco32I GATATC 2 cut(s) 1455, 3178
Eco47I GGWCC 4 cut(s) 764, 953, 1476, 3417
Eco57I CTGAAG 4 cut(s) 1067, 2549, 3222, 3389
Eco81I CCTNAGG 1 cut(s) 1698
Eco88I CYCGRG 2 cut(s) 590, 2456
EcoNI CCTNNNNNAGG 1 cut(s) 3407
EcoRII CCWGG 7 cut(s) 955, 1570, 1918, 2377, 2614, 2844, 3378
EcoRV GATATC 2 cut(s) 1455, 3178
EcoT14I CCWWGG 3 cut(s) 818, 1179, 1961
EcoT22I ATGCAT 5 cut(s) 608, 993, 1312, 1402, 1515
ErhI CCWWGG 3 cut(s) 818, 1179, 1961
FalI AAGNNNNNCTT 4 cut(s) 2226, 2258, 2965, 2997
FaqI GGGAC 3 cut(s) 2209, 2624, 3333
FauI CCCGC 2 cut(s) 1162, 1487
FauNDI CATATG 1 cut(s) 3088
FbaI TGATCA 3 cut(s) 937, 1129, 3583
Fnu4HI GCNGC 9 cut(s) 105, 369, 372, 532, 1120, 1167, 2000, 2266, 2914
Fsp4HI GCNGC 9 cut(s) 105, 369, 372, 532, 1120, 1167, 2000, 2266, 2914
FspBI CTAG 5 cut(s) 449, 981, 1064, 1962, 2484
FspI TGCGCA 1 cut(s) 27
GlaI GCGC 6 cut(s) 27, 180, 535, 1225, 1580, 2858
GluI GCNGC 9 cut(s) 105, 369, 372, 532, 1120, 1167, 2000, 2266, 2914
GsaI CCCAGC 2 cut(s) 1999, 2196
GsuI CTGGAG 2 cut(s) 1916, 2828
HaeII RGCGCY 2 cut(s) 1582, 2860
HaeIII GGCC 5 cut(s) 1184, 1703, 1852, 1953, 3546
HapII CCGG 1 cut(s) 2137
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 6 cut(s) 28, 181, 536, 1226, 1581, 2859
Hin6I GCGC 6 cut(s) 26, 179, 534, 1224, 1579, 2857
HinP1I GCGC 6 cut(s) 26, 179, 534, 1224, 1579, 2857
HincII GTYRAC 1 cut(s) 3244
HindII GTYRAC 1 cut(s) 3244
HindIII AAGCTT 5 cut(s) 206, 717, 2108, 3013, 3597
HpaII CCGG 1 cut(s) 2137
HphI GGTGA 7 cut(s) 77, 947, 1757, 3290, 3317, 3368, 3520
Hpy166II GTNNAC 6 cut(s) 1593, 1795, 2035, 2943, 3244, 3420
Hpy8I GTNNAC 6 cut(s) 1593, 1795, 2035, 2943, 3244, 3420
Hpy99I CGWCG 4 cut(s) 1079, 1215, 2665, 2668
HpyCH4III ACNGT 3 cut(s) 637, 1906, 2932
HpyCH4IV ACGT 3 cut(s) 2524, 2586, 3104
HpySE526I ACGT 3 cut(s) 2524, 2586, 3104
HspAI GCGC 6 cut(s) 26, 179, 534, 1224, 1579, 2857
Ksp22I TGATCA 3 cut(s) 937, 1129, 3583
KspI CCGCGG 1 cut(s) 1599
LguI GCTCTTC 1 cut(s) 1094
LmnI GCTCC 3 cut(s) 2512, 2793, 2883
Lsp1109I GCAGC 7 cut(s) 358, 380, 1106, 1153, 2011, 2252, 2900
MabI ACCWGGT 1 cut(s) 3378
MaeI CTAG 5 cut(s) 449, 981, 1064, 1962, 2484
MaeII ACGT 3 cut(s) 2524, 2586, 3104
MaeIII GTNAC 8 cut(s) 77, 225, 517, 910, 1499, 1988, 2610, 2705
MfeI CAATTG 1 cut(s) 2894
MflI RGATCY 8 cut(s) 331, 1558, 1810, 2017, 2505, 2727, 3164, 3205
MhlI GDGCHC 2 cut(s) 414, 1797
MlyI GAGTC 5 cut(s) 602, 693, 1687, 1923, 2642
MmeI TCCRAC 3 cut(s) 544, 1757, 2252
Mph1103I ATGCAT 5 cut(s) 608, 993, 1312, 1402, 1515
MroXI GAANNNNTTC 7 cut(s) 621, 1262, 1644, 1784, 1821, 2100, 3285
MslI CAYNNNNRTG 4 cut(s) 99, 601, 1401, 3459
MspA1I CMGCKG 5 cut(s) 371, 1119, 1598, 1848, 2002
MspI CCGG 1 cut(s) 2137
MspR9I CCNGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
MunI CAATTG 1 cut(s) 2894
Mva1269I GAATGC 2 cut(s) 287, 3031
MvaI CCWGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
MvnI CGCG 3 cut(s) 534, 1482, 1598
NdeI CATATG 1 cut(s) 3088
NlaIV GGNNCC 5 cut(s) 1201, 1478, 2135, 2514, 2782
NmeAIII GCCGAG 1 cut(s) 2803
NmuCI GTSAC 3 cut(s) 225, 517, 1988
NsbI TGCGCA 1 cut(s) 27
NsiI ATGCAT 5 cut(s) 608, 993, 1312, 1402, 1515
NspI RCATGY 6 cut(s) 32, 344, 540, 1400, 1404, 3273
NspV TTCGAA 1 cut(s) 2490
PaeI GCATGC 2 cut(s) 32, 540
PaeR7I CTCGAG 2 cut(s) 590, 2456
PagI TCATGA 1 cut(s) 1069
PaqCI CACCTGC 1 cut(s) 184
PasI CCCWGGG 1 cut(s) 2378
PceI AGGCCT 1 cut(s) 1703
PciI ACATGT 2 cut(s) 340, 3269
PciSI GCTCTTC 1 cut(s) 1094
PcsI WCGNNNNNNNCGW 1 cut(s) 1434
PctI GAATGC 2 cut(s) 287, 3031
PdmI GAANNNNTTC 7 cut(s) 621, 1262, 1644, 1784, 1821, 2100, 3285
Pfl23II CGTACG 1 cut(s) 3105
PflFI GACNNNGTC 1 cut(s) 2932
PflMI CCANNNNNTGG 1 cut(s) 2002
PfoI TCCNGGA 1 cut(s) 2844
PkrI GCNGC 9 cut(s) 106, 370, 373, 533, 1121, 1168, 2001, 2267, 2915
PleI GAGTC 5 cut(s) 601, 693, 1687, 1923, 2641
PpsI GAGTC 5 cut(s) 601, 693, 1687, 1923, 2641
Ppu21I YACGTR 1 cut(s) 3105
PscI ACATGT 2 cut(s) 340, 3269
PshAI GACNNNNGTC 1 cut(s) 1608
Psp6I CCWGG 7 cut(s) 955, 1570, 1918, 2377, 2614, 2844, 3378
PspFI CCCAGC 2 cut(s) 1995, 2192
PspGI CCWGG 7 cut(s) 955, 1570, 1918, 2377, 2614, 2844, 3378
PspLI CGTACG 1 cut(s) 3105
PspN4I GGNNCC 5 cut(s) 1201, 1478, 2135, 2514, 2782
PspPI GGNCC 5 cut(s) 764, 953, 1183, 1476, 3417
PspXI VCTCGAGB 1 cut(s) 590
PsrI GAACNNNNNNTAC 2 cut(s) 606, 638
PstI CTGCAG 2 cut(s) 1020, 1894
PstNI CAGNNNCTG 2 cut(s) 2002, 2936
PsuI RGATCY 8 cut(s) 331, 1558, 1810, 2017, 2505, 2727, 3164, 3205
PsyI GACNNNGTC 1 cut(s) 2932
PvuII CAGCTG 4 cut(s) 371, 1119, 1848, 2002
RseI CAYNNNNRTG 4 cut(s) 99, 601, 1401, 3459
SacII CCGCGG 1 cut(s) 1599
SapI GCTCTTC 1 cut(s) 1094
SatI GCNGC 9 cut(s) 105, 369, 372, 532, 1120, 1167, 2000, 2266, 2914
Sau96I GGNCC 5 cut(s) 764, 953, 1183, 1476, 3417
SchI GAGTC 5 cut(s) 602, 693, 1687, 1923, 2642
ScrFI CCNGG 7 cut(s) 957, 1572, 1920, 2379, 2616, 2846, 3380
SduI GDGCHC 2 cut(s) 414, 1797
SexAI ACCWGGT 1 cut(s) 3378
SfcI CTRYAG 2 cut(s) 1016, 1890
Sfr274I CTCGAG 2 cut(s) 590, 2456
Sfr303I CCGCGG 1 cut(s) 1599
SfuI TTCGAA 1 cut(s) 2490
SgrBI CCGCGG 1 cut(s) 1599
SinI GGWCC 4 cut(s) 764, 953, 1476, 3417
SlaI CTCGAG 2 cut(s) 590, 2456
SmiMI CAYNNNNRTG 4 cut(s) 99, 601, 1401, 3459
SmlI CTYRAG 8 cut(s) 413, 541, 590, 2232, 2456, 2630, 3441, 3540
SmoI CTYRAG 8 cut(s) 413, 541, 590, 2232, 2456, 2630, 3441, 3540
SnaBI TACGTA 1 cut(s) 3105
SphI GCATGC 2 cut(s) 32, 540
SseBI AGGCCT 1 cut(s) 1703
SsiI CCGC 9 cut(s) 104, 532, 1169, 1415, 1474, 1480, 1596, 1598, 2596
SspI AATATT 1 cut(s) 1871
SspMI CTAG 5 cut(s) 449, 981, 1064, 1962, 2484
StuI AGGCCT 1 cut(s) 1703
StyD4I CCNGG 7 cut(s) 955, 1570, 1918, 2377, 2614, 2844, 3378
StyI CCWWGG 3 cut(s) 818, 1179, 1961
TaaI ACNGT 3 cut(s) 637, 1906, 2932
TaiI ACGT 3 cut(s) 2527, 2589, 3107
TaqII GACCGA 2 cut(s) 2656, 3567
TatI WGTACW 2 cut(s) 2336, 3091
TauI GCSGC 2 cut(s) 107, 534
TscAI CASTG 3 cut(s) 640, 1384, 2937
TseFI GTSAC 3 cut(s) 225, 517, 1988
TseI GCWGC 7 cut(s) 368, 371, 1119, 1166, 1999, 2265, 2913
Tsp45I GTSAC 3 cut(s) 225, 517, 1988
TspGWI ACGGA 3 cut(s) 2649, 3239, 3481
TspRI CASTG 3 cut(s) 640, 1384, 2937
Tth111I GACNNNGTC 1 cut(s) 2932
Van91I CCANNNNNTGG 1 cut(s) 2002
VneI GTGCAC 1 cut(s) 1793
VpaK11BI GGWCC 4 cut(s) 764, 953, 1476, 3417
XagI CCTNNNNNAGG 1 cut(s) 3407
XapI RAATTY 5 cut(s) 1191, 1727, 2388, 2555, 3132
XceI RCATGY 6 cut(s) 32, 344, 540, 1400, 1404, 3273
XcmI CCANNNNNNNNNTGG 1 cut(s) 2295
XhoI CTCGAG 2 cut(s) 590, 2456
XmaJI CCTAGG 1 cut(s) 1961
XmnI GAANNNNTTC 7 cut(s) 621, 1262, 1644, 1784, 1821, 2100, 3285
XspI CTAG 5 cut(s) 449, 981, 1064, 1962, 2484
Zsp2I ATGCAT 5 cut(s) 608, 993, 1312, 1402, 1515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.