Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0010076

Physical Location & Seq

Genomic Coordinates Reverse (-)

31134533 .. 31137460

Gene Structure 2928 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0275700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGGCTCTGGAAGTTGTGGGTGGAGCTTTTCTCTCTTCTTCTCTTGCTGTTCTGTTTGATAGGATGGCTTCCCGTCCGGTTGTTGACTTCATCCGAGGAAAGAAGACCGCCGCTGGGCTGCTCCACAAGTTGAAGATTAAGTTGTTGTCGGTTAATAAGTTGCTTGATGATGCAGAGGAGAAGCAACTCAGAAACTCTAGAGTCAGGGAATGGCTTAATGAGCTTAAAGATGTTCTCTATCATGCCGATGACCTTTTGGATGAAATCAAGACTGAAGGTCTGCGTTGCAAGATGGAACAAGAAGATTCTGGAAGCATCAGCACAAATCAGGTACCCGAGTTTAGTTCTATTTCCATTCATGAACTTGACGAAAGTCTGGAACCCAGGATTCTGGACATTCTTGACAGATTAGAACTTATTGTCAACGAAAAAGATGTGCTTGATTTGAAAGAGGGTGTTAAAGATAGACCACAAGGAAGACTGCCCACAACTTCTTTGGTAGAAGAGTCTAGTGTATATGGAAGGGATGAAGAAAAGGAGGCCATTATCAAGTTATTGTTAGCGGATGGTATGACTGGAAATAAGACAGATGTGCTTCCAATTGTGGGCATGGGTGGGCTTGGAAAGACCACTCTTGCTCAACTCGTATACAACGATGACAGAGTCAAGCAACATTTTGTCCATCAATCATGGTCTTGTGTTTCAGTAGAATTTGATGTTATCAAAATAACACAGACACTTTATGGGGCGGTCACTTCACAAACTCGTAATATCACAGACCTAAACATGCTTCAAGTTAAGCTAAAGGAGACGTTGTCAGGAAAGAAATTTCTCTTTGTTCTTGATGATGTTTGGACTGTGACTAATGACGAGTGGGATCTCTTAAGCCGACCCTTTGAGAGTGGAGGTCATGGAAGTAAGATCATTGTCACAACACGTCATGAAGATGTTGCAGGCATTATAGGTACCCTTCAAAGTCAACATCTAATGCAACTATCTGAGGAAAACTGCTGGTCGTTGTTCTCAAAACATGCCTTCAAGAATGCAGGTGTTACTGCAGATCTGTCGCTTGAAGTGATTGGAAAACAGATTGCTCAAAAGTGTAAAGGACTCCCTTTAGCTGCAAAATCACTTGGGGGTCTTTTACGTTCTGAACCAAATATTGAGAAATGGGAAGAAGTGTTGAACAATGACATATGGGAGTTGCTAGATAAAAAGAATGGCATTTTGCCAGCTCTATGGTTGAGCTACCATTATCTACCTCCGCATCTGAAGCGTTGTTTTGCTTACTGTTCCATATTTCCTCAAGATTATCGGTTTCAGAAATCGGATCTGGTGTTGCTGTGGATGGCTGAAGATCTCTTACCGTCGAAATCGAAGACTACATTAGAACAAGTCGGAGAGAACTACTTTGATGATCTAGTGTCAAGGTCATTTTTTCAACATGTACCACATTCGGTTTGGCCAATTTTCACAATGCATGACCTTATCCATGACTTAGCAAACTTTGTCTCTGGAGAGTTTTGTTTTAGGTTGGAGAACAATGATGAGGTATTGGAAAATGTCAGCAAGACTCGCCACTTTTCGTTTATGGATGCATCTACCGTAATTGATCAATGGGACAGTTTGTGTGAAGCCAAGTATTTGCGAACCTTTCTAGTATCAAAATATCAGTACGTAGGTCCCAACTTAGATTTTGATATATTCCTGAAGCTTCAGTGTTTAAGAGTGCTCAATTTATCAAGGTACAATATTGGTGAGCTGCCTAGTTCAATTAGCAAGTTGAAGCATCTAAGGCACTTTGACTTGTCTCGCACATCAATTAAAAAGTTACCTGATGAAATGTGTACTTTATATAATTTACAGACGTTGTTGTTGTTCTATTGTGACTCTCTAGTTGAGTTGCCAACTGATTTGGGAAGATTAATCAACTTGCGTCATCTTGATATTAGAAGCACACAGATAAAAAAGATGCCACCAAGGATGGGGAAAATGAAAGATCTCCAAACAATAAGAGGTAAGTTTGTCCTAGACAAATACACTGGTGGTAACATTGTCGAGATTAAAGAGCTTCAGCAGTTGCGTGGAACACTATGCATCTCGGGGCTTCGCAATATTGTGCATGTAACAGATGCCTTGGAGACCAATATCAGGAAGAAGAAGTATTTGGATGGATTAGTTTTGGAATGGGGAGGCAAGTCTGGTGACACCGACGATACGCAAAAAGATAGAGATGTGCTGGAGAACCTCCAACCTCATACAAACCTAAAAAAACTCGAGATTCGATCTTATGGCGGCACAAGATTTCCAGGTTGGTTGGAAGATCTTTCTAGTTTATCTAATCTGGTTCGTCTTCGACTTCTGGACTGCGGAAATTGTTTGTCCTTGCCATCAGTTGAGCAGCTACCCTCCCTACAAGAGCTTTGGATTGAAGGATTTAATGGAGTGGTGACTGTGGGTTCTAAGCCTTTTCCTAATCTTCGTAAGCTTCGTCTGAAATCTTGTCCCGAGGTAACCGGGGCATTCATGTTAGCCTGCTTCCCAAAGCTTGAAAGCCTCGATTTGGAGGATGTGAATGTAGAATCTCTTCATTCCACTACAGTACTCCTCTCCCTTGTCGAACTCACAATATCTTCATGCCCAAATTTTGTTTGTTTTCAAGATGGAGGGCTGAATGCGCCCAACTTGTGGTCCATAAGAGTCCAGAAATGTCCGAAACTGAGGTCATTTTTTCCGCAACACGTGGACAACCTTCTCCCATCTCTGGGGTTGTTGTTGGCAAATGATTGTCCAGAATTGGAATCATTTCCCGAAGGGGGATTGCCGTCTAAGTTGAAATTCTTGTACATCTGGCGTTGCAAGAAACTCCCCTCATGCAGTGGGGTCTACCAACACTCACCTCTCTTGAAATGTTGTGGGTCGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006520 GO:0006536 GO:0006541 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0008150 GO:0008152 GO:0008509 GO:0008514 GO:0009064 GO:0009507 GO:0009526 GO:0009534 GO:0009536 GO:0009579 GO:0009605 GO:0009607 GO:0009624 GO:0009941 GO:0009987 GO:0015075 GO:0015131 GO:0015139 GO:0015140 GO:0015318 GO:0015556 GO:0015711 GO:0015729 GO:0015740 GO:0015742 GO:0015743 GO:0015849 GO:0016020 GO:0019676 GO:0019740 GO:0019752 GO:0022857 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034220 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0050896 GO:0051179 GO:0051181 GO:0051184 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0071423 GO:0071702 GO:0071704 GO:0098656 GO:1901564 GO:1901605 GO:1902356 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_sulph_symp

Protein Analysis

975

Amino Acids

110.46

Weight (kDa)

6.11

Isoelectric Point (pI)

43.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	11 - 100	1.8e-17	Rx N-terminal domain
NB-ARC	PF00931	177 - 348	1.6e-35	NB-ARC domain
WHD_DRP	PF23559	433 - 501	5.6e-25	Disease resistance protein Winged helix domain
LRR_14	PF23598	571 - 865	1.6e-29	Leucine-rich repeat region
LRR_R13L1-DRL21	PF25019	685 - 812	6.1e-39	R13L1/DRL21 LRRs
LRR_RPS2	PF23247	805 - 930	5.2e-06	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010076)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12860 AT5G12860
fragaria_vesca	FvH4_3g28880
malus_domestica	MD03G1175800.v1.1 MD11G1193500.v1.1
prunus_persica	Prupe.4G248000_v2.0.a1
pyrus_communis	pycom03g13320 pycom11g16910
rosa_chinensis	RchiOBHm_Chr5g0051981
rosa_laevigata	RLG00000034849
rosa_multiflora	Rmu_co8424073.1_g000002 Rmu_ssc0000466.1_g000003
rosa_roxburghii	Rroxscaffold_1G00027750
rosa_rugosa	Rorug05G0275700 Rorug05G0275800
rosa_samantha	Rh5AG347500 Rh5BG358500 Rh5CG383900 Rh5DG374000 Rh7CG246800
rosa_wichuraiana	Rw5G032850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1037
Acc36I	ACCTGC	1 cut(s)	1037
Acc65I	GGTACC	2 cut(s)	331, 965
AccB1I	GGYRCC	2 cut(s)	331, 965
AccB7I	CCANNNNNTGG	1 cut(s)	2688
AccI	GTMKAC	3 cut(s)	648, 2886, 2921
AciI	CCGC	8 cut(s)	108, 111, 563, 749, 1265, 2295, 2370, 2735
AclWI	GGATC	2 cut(s)	885, 1338
AcoI	YGGCCR	1 cut(s)	1463
AcsI	RAATTY	4 cut(s)	710, 827, 2644, 2837
AcuI	CTGAAG	6 cut(s)	294, 1292, 1374, 1700, 1730, 2057
AcvI	CACGTG	1 cut(s)	2743
AfaI	GTAC	8 cut(s)	333, 967, 1449, 1676, 1748, 1849, 2604, 2846
AfiI	CCNNNNNNNGG	9 cut(s)	114, 605, 1985, 2151, 2563, 2688, 2764, 2765, 2816
AflII	CTTAAG	1 cut(s)	883
AflIII	ACRYGT	3 cut(s)	935, 1444, 2740
AhdI	GACNNNNNGTC	1 cut(s)	276
AjiI	CACGTC	1 cut(s)	938
AjnI	CCWGG	2 cut(s)	383, 2308
AjuI	GAANNNNNNNTTGG	2 cut(s)	2150, 2182
Alw21I	GWGCWC	1 cut(s)	1734
Alw26I	GTCTC	4 cut(s)	803, 1516, 1815, 2135
AlwI	GGATC	2 cut(s)	885, 1338
Ama87I	CYCGRG	4 cut(s)	335, 2101, 2276, 2507
AoxI	GGCC	2 cut(s)	540, 1463
ApeKI	GCWGC	4 cut(s)	118, 1121, 1762, 2401
ApoI	RAATTY	4 cut(s)	710, 827, 2644, 2837
ArsI	GACNNNNNNTTYG	2 cut(s)	2708, 2740
AseI	ATTAAT	1 cut(s)	1925
Asp700I	GAANNNNTTC	2 cut(s)	2586, 2805
Asp718I	GGTACC	2 cut(s)	331, 965
AspLEI	GCGC	1 cut(s)	2680
AspS9I	GGNCC	2 cut(s)	1682, 2691
AsuC2I	CCSGG	1 cut(s)	2518
AsuHPI	GGTGA	4 cut(s)	1769, 2216, 2461, 2889
AvaI	CYCGRG	4 cut(s)	335, 2101, 2276, 2507
AvaII	GGWCC	2 cut(s)	1682, 2691
BaeI	ACNNNNGTAYC	2 cut(s)	323, 356
BalI	TGGCCA	1 cut(s)	1465
BanI	GGYRCC	2 cut(s)	331, 965
BarI	GAAGNNNNNNTAC	4 cut(s)	1347, 1379, 2571, 2603
BbrPI	CACGTG	1 cut(s)	2743
BbsI	GAAGAC	4 cut(s)	110, 484, 1385, 2345
Bbv12I	GWGCWC	1 cut(s)	1734
BbvI	GCAGC	4 cut(s)	105, 1108, 1749, 2413
BceAI	ACGGC	1 cut(s)	2809
BcgI	CGANNNNNNTGC	2 cut(s)	1047, 1081
BciT130I	CCWGG	2 cut(s)	385, 2310
BclI	TGATCA	1 cut(s)	1612
BcnI	CCSGG	1 cut(s)	2518
BcoDI	GTCTC	4 cut(s)	803, 1516, 1815, 2135
BfaI	CTAG	9 cut(s)	198, 510, 1208, 1421, 1658, 1767, 1895, 2030, 2331
BfmI	CTRYAG	2 cut(s)	1056, 2598
BfrI	CTTAAG	1 cut(s)	883
BfuAI	ACCTGC	1 cut(s)	1037
BglII	AGATCT	4 cut(s)	1060, 1357, 1999, 2323
BisI	GCNGC	6 cut(s)	111, 119, 1122, 1763, 2296, 2402
BlsI	GCNGC	6 cut(s)	112, 120, 1123, 1764, 2297, 2403
BmcAI	AGTACT	1 cut(s)	2604
Bme1390I	CCNGG	3 cut(s)	385, 2310, 2518
Bme18I	GGWCC	2 cut(s)	1682, 2691
BmeRI	GACNNNNNGTC	1 cut(s)	276
BmeT110I	CYCGRG	4 cut(s)	335, 2101, 2276, 2507
BmgBI	CACGTC	1 cut(s)	938
BmgT120I	GGNCC	2 cut(s)	1682, 2691
BmiI	GGNNCC	4 cut(s)	333, 381, 967, 1684
BmrFI	CCNGG	3 cut(s)	385, 2310, 2518
BmsI	GCATC	9 cut(s)	160, 324, 1276, 1585, 1607, 1798, 1962, 2106, 2122
BoxI	GACNNNNGTC	1 cut(s)	372
BpiI	GAAGAC	4 cut(s)	110, 484, 1385, 2345
BplI	GAGNNNNNCTC	2 cut(s)	15, 47
BpmI	CTGGAG	2 cut(s)	1536, 2261
BpuEI	CTTGAG	1 cut(s)	1290
BpuMI	CCSGG	1 cut(s)	2518
BsaAI	YACGTR	2 cut(s)	1678, 2743
BsaI	GGTCTC	1 cut(s)	2135
BsaJI	CCNNGG	6 cut(s)	94, 383, 1979, 2136, 2508, 2517
BsaWI	WCCGGW	1 cut(s)	76
BsaXI	ACNNNNNCTCC	2 cut(s)	800, 830
Bsc4I	CCNNNNNNNGG	9 cut(s)	114, 605, 1985, 2151, 2563, 2688, 2764, 2765, 2816
Bse1I	ACTGG	2 cut(s)	580, 2047
BseBI	CCWGG	2 cut(s)	385, 2310
BseDI	CCNNGG	6 cut(s)	94, 383, 1979, 2136, 2508, 2517
BseLI	CCNNNNNNNGG	9 cut(s)	114, 605, 1985, 2151, 2563, 2688, 2764, 2765, 2816
BseMII	CTCAG	3 cut(s)	202, 990, 2711
BseNI	ACTGG	2 cut(s)	580, 2047
BseRI	GAGGAG	2 cut(s)	191, 2597
BseXI	GCAGC	4 cut(s)	105, 1108, 1749, 2413
BseYI	CCCAGC	1 cut(s)	113
BshFI	GGCC	2 cut(s)	542, 1465
BshNI	GGYRCC	2 cut(s)	331, 965
BsiHKAI	GWGCWC	1 cut(s)	1734
BsiHKCI	CYCGRG	4 cut(s)	335, 2101, 2276, 2507
BsiSI	CCGG	2 cut(s)	77, 2517
BslFI	GGGAC	3 cut(s)	1634, 1668, 2490
BslI	CCNNNNNNNGG	9 cut(s)	114, 605, 1985, 2151, 2563, 2688, 2764, 2765, 2816
BsmAI	GTCTC	4 cut(s)	803, 1516, 1815, 2135
BsmBI	CGTCTC	1 cut(s)	803
BsmFI	GGGAC	3 cut(s)	1634, 1668, 2490
BsmI	GAATGC	3 cut(s)	1048, 2522, 2680
BsnI	GGCC	2 cut(s)	542, 1465
Bso31I	GGTCTC	1 cut(s)	2135
BsoBI	CYCGRG	4 cut(s)	335, 2101, 2276, 2507
Bsp1286I	GDGCHC	1 cut(s)	1734
Bsp1407I	TGTACA	1 cut(s)	2844
BspACI	CCGC	8 cut(s)	108, 111, 563, 749, 1265, 2295, 2370, 2735
BspANI	GGCC	2 cut(s)	542, 1465
BspCNI	CTCAG	3 cut(s)	201, 991, 2712
BspHI	TCATGA	2 cut(s)	358, 940
BspLI	GGNNCC	4 cut(s)	333, 381, 967, 1684
BspMAI	CTGCAG	1 cut(s)	1060
BspMI	ACCTGC	1 cut(s)	1037
BspPI	GGATC	2 cut(s)	885, 1338
BspT107I	GGYRCC	2 cut(s)	331, 965
BspTI	CTTAAG	1 cut(s)	883
BspTNI	GGTCTC	1 cut(s)	2135
BsrGI	TGTACA	1 cut(s)	2844
BsrI	ACTGG	2 cut(s)	580, 2047
BssECI	CCNNGG	6 cut(s)	94, 383, 1979, 2136, 2508, 2517
BssNAI	GTATAC	1 cut(s)	649
BssT1I	CCWWGG	2 cut(s)	1979, 2136
Bst1107I	GTATAC	1 cut(s)	649
Bst2UI	CCWGG	2 cut(s)	385, 2310
Bst4CI	ACNGT	7 cut(s)	859, 1292, 1368, 1606, 1625, 2455, 2602
Bst6I	CTCTTC	3 cut(s)	40, 498, 2592
BstAFI	CTTAAG	1 cut(s)	883
BstAUI	TGTACA	1 cut(s)	2844
BstBAI	YACGTR	2 cut(s)	1678, 2743
BstC8I	GCNNGC	3 cut(s)	955, 1233, 2536
BstDEI	CTNAG	8 cut(s)	188, 999, 1498, 1690, 1793, 2463, 2720, 2829
BstEII	GGTNACC	1 cut(s)	2512
BstHHI	GCGC	1 cut(s)	2680
BstMAI	GTCTC	4 cut(s)	803, 1516, 1815, 2135
BstMWI	GCNNNNNNNGC	5 cut(s)	220, 1273, 1575, 1795, 2677
BstNI	CCWGG	2 cut(s)	385, 2310
BstNSI	RCATGY	4 cut(s)	790, 1034, 1448, 2126
BstPAI	GACNNNNGTC	1 cut(s)	372
BstPI	GGTNACC	1 cut(s)	2512
BstSCI	CCNGG	3 cut(s)	383, 2308, 2516
BstSFI	CTRYAG	2 cut(s)	1056, 2598
BstSNI	TACGTA	1 cut(s)	1678
BstV1I	GCAGC	4 cut(s)	105, 1108, 1749, 2413
BstV2I	GAAGAC	4 cut(s)	110, 484, 1385, 2345
BstX2I	RGATCY	6 cut(s)	877, 1060, 1330, 1357, 1999, 2323
BstXI	CCANNNNNNTGG	2 cut(s)	391, 1239
BstYI	RGATCY	6 cut(s)	877, 1060, 1330, 1357, 1999, 2323
BstZ17I	GTATAC	1 cut(s)	649
BsuRI	GGCC	2 cut(s)	542, 1465
BtrI	CACGTC	1 cut(s)	938
BtsI	GCAGTG	1 cut(s)	2884
BtsIMutI	CAGTG	3 cut(s)	1724, 2040, 2884
BveI	ACCTGC	1 cut(s)	1037
Cac8I	GCNNGC	3 cut(s)	955, 1233, 2536
CciI	TCATGA	2 cut(s)	358, 940
CfoI	GCGC	1 cut(s)	2680
Cfr13I	GGNCC	2 cut(s)	1682, 2691
CseI	GACGC	1 cut(s)	1925
Csp6I	GTAC	8 cut(s)	332, 966, 1448, 1675, 1747, 1848, 2603, 2845
CviQI	GTAC	8 cut(s)	332, 966, 1448, 1675, 1747, 1848, 2603, 2845
DdeI	CTNAG	8 cut(s)	188, 999, 1498, 1690, 1793, 2463, 2720, 2829
DriI	GACNNNNNGTC	1 cut(s)	276
EaeI	YGGCCR	1 cut(s)	1463
Eam1104I	CTCTTC	3 cut(s)	40, 498, 2592
Eam1105I	GACNNNNNGTC	1 cut(s)	276
EarI	CTCTTC	3 cut(s)	40, 498, 2592
Eco105I	TACGTA	1 cut(s)	1678
Eco130I	CCWWGG	2 cut(s)	1979, 2136
Eco31I	GGTCTC	1 cut(s)	2135
Eco47I	GGWCC	2 cut(s)	1682, 2691
Eco57I	CTGAAG	6 cut(s)	294, 1292, 1374, 1700, 1730, 2057
Eco72I	CACGTG	1 cut(s)	2743
Eco88I	CYCGRG	4 cut(s)	335, 2101, 2276, 2507
Eco91I	GGTNACC	1 cut(s)	2512
EcoO109I	RGGNCCY	1 cut(s)	1682
EcoO65I	GGTNACC	1 cut(s)	2512
EcoRII	CCWGG	2 cut(s)	383, 2308
EcoT14I	CCWWGG	2 cut(s)	1979, 2136
EcoT22I	ATGCAT	3 cut(s)	1482, 1600, 2099
ErhI	CCWWGG	2 cut(s)	1979, 2136
Esp3I	CGTCTC	1 cut(s)	803
FalI	AAGNNNNNCTT	2 cut(s)	423, 455
FaqI	GGGAC	3 cut(s)	1634, 1668, 2490
FauNDI	CATATG	1 cut(s)	1196
FbaI	TGATCA	1 cut(s)	1612
FblI	GTMKAC	3 cut(s)	648, 2886, 2921
Fnu4HI	GCNGC	6 cut(s)	111, 119, 1122, 1763, 2296, 2402
Fsp4HI	GCNGC	6 cut(s)	111, 119, 1122, 1763, 2296, 2402
FspBI	CTAG	9 cut(s)	198, 510, 1208, 1421, 1658, 1767, 1895, 2030, 2331
GlaI	GCGC	1 cut(s)	2679
GluI	GCNGC	6 cut(s)	111, 119, 1122, 1763, 2296, 2402
GsaI	CCCAGC	1 cut(s)	117
GsuI	CTGGAG	2 cut(s)	1536, 2261
HaeIII	GGCC	2 cut(s)	542, 1465
HapII	CCGG	2 cut(s)	77, 2517
HgaI	GACGC	1 cut(s)	1925
HhaI	GCGC	1 cut(s)	2680
Hin6I	GCGC	1 cut(s)	2678
HinP1I	GCGC	1 cut(s)	2678
HincII	GTYRAC	4 cut(s)	85, 424, 980, 2922
HindII	GTYRAC	4 cut(s)	85, 424, 980, 2922
HindIII	AAGCTT	3 cut(s)	1712, 2486, 2546
HpaII	CCGG	2 cut(s)	77, 2517
HphI	GGTGA	4 cut(s)	1769, 2216, 2461, 2889
Hpy166II	GTNNAC	8 cut(s)	85, 424, 649, 980, 1848, 2746, 2887, 2922
Hpy8I	GTNNAC	8 cut(s)	85, 424, 649, 980, 1848, 2746, 2887, 2922
Hpy99I	CGWCG	2 cut(s)	1372, 2216
HpyAV	CCTTC	7 cut(s)	269, 516, 980, 1045, 2426, 2762, 2807
HpyCH4III	ACNGT	7 cut(s)	859, 1292, 1368, 1606, 1625, 2455, 2602
HpyCH4IV	ACGT	6 cut(s)	812, 937, 1147, 1677, 1868, 2742
HpyF10VI	GCNNNNNNNGC	5 cut(s)	220, 1273, 1575, 1795, 2677
HpyF3I	CTNAG	8 cut(s)	188, 999, 1498, 1690, 1793, 2463, 2720, 2829
HpySE526I	ACGT	6 cut(s)	812, 937, 1147, 1677, 1868, 2742
HspAI	GCGC	1 cut(s)	2678
KpnI	GGTACC	2 cut(s)	335, 969
Ksp22I	TGATCA	1 cut(s)	1612
LmnI	GCTCC	2 cut(s)	23, 126
Lsp1109I	GCAGC	4 cut(s)	105, 1108, 1749, 2413
LweI	GCATC	9 cut(s)	160, 324, 1276, 1585, 1607, 1798, 1962, 2106, 2122
MaeI	CTAG	9 cut(s)	198, 510, 1208, 1421, 1658, 1767, 1895, 2030, 2331
MaeII	ACGT	6 cut(s)	812, 937, 1147, 1677, 1868, 2742
MfeI	CAATTG	1 cut(s)	600
MflI	RGATCY	6 cut(s)	877, 1060, 1330, 1357, 1999, 2323
MhlI	GDGCHC	1 cut(s)	1734
MlsI	TGGCCA	1 cut(s)	1465
MluNI	TGGCCA	1 cut(s)	1465
MlyI	GAGTC	7 cut(s)	210, 515, 672, 1104, 1567, 1883, 2709
MmeI	TCCRAC	4 cut(s)	1378, 1515, 2275, 2298
Mox20I	TGGCCA	1 cut(s)	1465
Mph1103I	ATGCAT	3 cut(s)	1482, 1600, 2099
MroXI	GAANNNNTTC	2 cut(s)	2586, 2805
MscI	TGGCCA	1 cut(s)	1465
MslI	CAYNNNNRTG	2 cut(s)	246, 945
Msp20I	TGGCCA	1 cut(s)	1465
MspA1I	CMGCKG	1 cut(s)	113
MspCI	CTTAAG	1 cut(s)	883
MspI	CCGG	2 cut(s)	77, 2517
MspR9I	CCNGG	3 cut(s)	385, 2310, 2518
MunI	CAATTG	1 cut(s)	600
Mva1269I	GAATGC	3 cut(s)	1048, 2522, 2680
MvaI	CCWGG	2 cut(s)	385, 2310
MwoI	GCNNNNNNNGC	5 cut(s)	220, 1273, 1575, 1795, 2677
NciI	CCSGG	1 cut(s)	2518
NdeI	CATATG	1 cut(s)	1196
NlaIV	GGNNCC	4 cut(s)	333, 381, 967, 1684
NmuCI	GTSAC	6 cut(s)	751, 859, 928, 1886, 2204, 2449
NsiI	ATGCAT	3 cut(s)	1482, 1600, 2099
NspI	RCATGY	4 cut(s)	790, 1034, 1448, 2126
PaeR7I	CTCGAG	1 cut(s)	2276
PagI	TCATGA	2 cut(s)	358, 940
PaqCI	CACCTGC	1 cut(s)	1037
PciI	ACATGT	1 cut(s)	1444
PcsI	WCGNNNNNNNCGW	2 cut(s)	651, 2488
PctI	GAATGC	3 cut(s)	1048, 2522, 2680
PdmI	GAANNNNTTC	2 cut(s)	2586, 2805
PfeI	GAWTC	5 cut(s)	305, 388, 2281, 2582, 2801
PflFI	GACNNNGTC	2 cut(s)	662, 814
PflMI	CCANNNNNTGG	1 cut(s)	2688
PkrI	GCNGC	6 cut(s)	112, 120, 1123, 1764, 2297, 2403
PleI	GAGTC	7 cut(s)	209, 514, 671, 1104, 1567, 1883, 2708
PmaCI	CACGTG	1 cut(s)	2743
PmlI	CACGTG	1 cut(s)	2743
PpsI	GAGTC	7 cut(s)	209, 514, 671, 1104, 1567, 1883, 2708
Ppu21I	YACGTR	2 cut(s)	1678, 2743
PpuMI	RGGWCCY	1 cut(s)	1682
PscI	ACATGT	1 cut(s)	1444
PshAI	GACNNNNGTC	1 cut(s)	372
PshBI	ATTAAT	1 cut(s)	1925
Psp5II	RGGWCCY	1 cut(s)	1682
Psp6I	CCWGG	2 cut(s)	383, 2308
PspCI	CACGTG	1 cut(s)	2743
PspEI	GGTNACC	1 cut(s)	2512
PspFI	CCCAGC	1 cut(s)	113
PspGI	CCWGG	2 cut(s)	383, 2308
PspN4I	GGNNCC	4 cut(s)	333, 381, 967, 1684
PspPI	GGNCC	2 cut(s)	1682, 2691
PspPPI	RGGWCCY	1 cut(s)	1682
PstI	CTGCAG	1 cut(s)	1060
PsuI	RGATCY	6 cut(s)	877, 1060, 1330, 1357, 1999, 2323
PsyI	GACNNNGTC	2 cut(s)	662, 814
RsaI	GTAC	8 cut(s)	333, 967, 1449, 1676, 1748, 1849, 2604, 2846
RsaNI	GTAC	8 cut(s)	332, 966, 1448, 1675, 1747, 1848, 2603, 2845
RseI	CAYNNNNRTG	2 cut(s)	246, 945
SalI	GTCGAC	1 cut(s)	2920
SatI	GCNGC	6 cut(s)	111, 119, 1122, 1763, 2296, 2402
Sau96I	GGNCC	2 cut(s)	1682, 2691
ScaI	AGTACT	1 cut(s)	2604
SchI	GAGTC	7 cut(s)	210, 515, 672, 1104, 1567, 1883, 2709
ScrFI	CCNGG	3 cut(s)	385, 2310, 2518
SduI	GDGCHC	1 cut(s)	1734
SfaNI	GCATC	9 cut(s)	160, 324, 1276, 1585, 1607, 1798, 1962, 2106, 2122
SfcI	CTRYAG	2 cut(s)	1056, 2598
Sfr274I	CTCGAG	1 cut(s)	2276
SinI	GGWCC	2 cut(s)	1682, 2691
SlaI	CTCGAG	1 cut(s)	2276
SmiMI	CAYNNNNRTG	2 cut(s)	246, 945
SmlI	CTYRAG	3 cut(s)	883, 1305, 2276
SmoI	CTYRAG	3 cut(s)	883, 1305, 2276
SnaBI	TACGTA	1 cut(s)	1678
SsiI	CCGC	8 cut(s)	108, 111, 563, 749, 1265, 2295, 2370, 2735
SspI	AATATT	3 cut(s)	1162, 1753, 2116
SspMI	CTAG	9 cut(s)	198, 510, 1208, 1421, 1658, 1767, 1895, 2030, 2331
StyD4I	CCNGG	3 cut(s)	383, 2308, 2516
StyI	CCWWGG	2 cut(s)	1979, 2136
TaaI	ACNGT	7 cut(s)	859, 1292, 1368, 1606, 1625, 2455, 2602
TaiI	ACGT	6 cut(s)	815, 940, 1150, 1680, 1871, 2745
TaqI	TCGA	9 cut(s)	1370, 1376, 2058, 2277, 2284, 2356, 2559, 2619, 2921
TatI	WGTACW	3 cut(s)	1847, 2602, 2844
TauI	GCSGC	2 cut(s)	113, 2298
TfiI	GAWTC	5 cut(s)	305, 388, 2281, 2582, 2801
TscAI	CASTG	3 cut(s)	1724, 2047, 2884
TseFI	GTSAC	6 cut(s)	751, 859, 928, 1886, 2204, 2449
TseI	GCWGC	4 cut(s)	118, 1121, 1762, 2401
Tsp45I	GTSAC	6 cut(s)	751, 859, 928, 1886, 2204, 2449
TspRI	CASTG	3 cut(s)	1724, 2047, 2884
Tth111I	GACNNNGTC	2 cut(s)	662, 814
Van91I	CCANNNNNTGG	1 cut(s)	2688
Vha464I	CTTAAG	1 cut(s)	883
VpaK11BI	GGWCC	2 cut(s)	1682, 2691
VspI	ATTAAT	1 cut(s)	1925
XapI	RAATTY	4 cut(s)	710, 827, 2644, 2837
XbaI	TCTAGA	1 cut(s)	197
XceI	RCATGY	4 cut(s)	790, 1034, 1448, 2126
XcmI	CCANNNNNNNNNTGG	2 cut(s)	493, 1458
XhoI	CTCGAG	1 cut(s)	2276
XmiI	GTMKAC	3 cut(s)	648, 2886, 2921
XmnI	GAANNNNTTC	2 cut(s)	2586, 2805
XspI	CTAG	9 cut(s)	198, 510, 1208, 1421, 1658, 1767, 1895, 2030, 2331
ZrmI	AGTACT	1 cut(s)	2604
Zsp2I	ATGCAT	3 cut(s)	1482, 1600, 2099

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.