Rorug05G0331900

Hydroxyacyl-thioester dehydratase type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009371
Physical Location & Seq
Reverse (-)
40186166 .. 40189062
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0331900.1

Sequence Viewer

Length: 396 bp
ATGTTGTATATGAAGACCGAGGATGGTAAACTTATTGAGATTAAACCATATGTTCGTATCCCAAGGACGTTTAAGCTATTCTCTGGTGTCATGTTGCAGCTGCCACAGAAACTGAGTATATCTGCCGTTGGTAAGCGTGAGAAACTTATGCGTATGATCAAGAACCCTATGACACAGTATTTACCTGTTAACTCCCGGAAAGTAGGACTCTCGTACAGTTCAGAGAAACTAGTTAGCATTCAAAAATATGTTGCTGCTGCTGAAAACAATATGGACCTTGTTTTTGTGGAGTATGGTGCATACAAGAAGGGAAGCTTTGACAAGATGCTTGCAGATGATAACACTCACAGGGTTCATATTCTTGCTCACATTTGCGCTGCTTGGCTATGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00630 ko00650 ko01100 ko01110 ko01120 ko01130 ko01200 ko03008 map00230 map00630 map00650 map01100 map01110 map01120 map01130 map01200 map03008
Pfam Domains
Protein Families
MaoC_dehydratas

Protein Analysis

131

Amino Acids

15.03

Weight (kDa)

9.54

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMG1 PF03587 2 - 129 3.4e-26 EMG1/NEP1 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 215
AgsI TTSAA 1 cut(s) 242
AhlI ACTAGT 1 cut(s) 229
AluBI AGCT 3 cut(s) 76, 100, 315
AluI AGCT 3 cut(s) 76, 100, 315
AlwNI CAGNNNCTG 1 cut(s) 112
ApeKI GCWGC 5 cut(s) 97, 100, 254, 257, 377
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 196
AvaII GGWCC 1 cut(s) 274
BbsI GAAGAC 1 cut(s) 20
BbvI GCAGC 5 cut(s) 87, 109, 241, 244, 364
BccI CCATC 1 cut(s) 17
BceAI ACGGC 1 cut(s) 110
BciVI GTATCC 1 cut(s) 68
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 1 cut(s) 196
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 68
BisI GCNGC 5 cut(s) 98, 101, 255, 258, 378
BlsI GCNGC 5 cut(s) 99, 102, 256, 259, 379
Bme1390I CCNGG 1 cut(s) 196
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 196
BmsI GCATC 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 18, 62
BseDI CCNNGG 2 cut(s) 18, 62
BseGI GGATG 1 cut(s) 28
BseMII CTCAG 1 cut(s) 104
BseXI GCAGC 5 cut(s) 87, 109, 241, 244, 364
BsiSI CCGG 1 cut(s) 196
BsmI GAATGC 1 cut(s) 237
Bsp143I GATC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 105
BssECI CCNNGG 2 cut(s) 18, 62
BssMI GATC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 62
Bst4CI ACNGT 2 cut(s) 177, 218
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 1 cut(s) 159
BstMBI GATC 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 194
BstV1I GCAGC 5 cut(s) 87, 109, 241, 244, 364
BstV2I GAAGAC 1 cut(s) 20
BsuI GTATCC 1 cut(s) 68
BtsCI GGATG 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 214
CviAII CATG 1 cut(s) 91
CviJI RGCY 4 cut(s) 76, 100, 315, 385
CviKI_1 RGCY 4 cut(s) 76, 100, 315, 385
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 113
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 62
FaeI CATG 1 cut(s) 94
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 1 cut(s) 90
FauNDI CATATG 1 cut(s) 49
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 5 cut(s) 98, 101, 255, 258, 378
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 5 cut(s) 98, 101, 255, 258, 378
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 376
GluI GCNGC 5 cut(s) 98, 101, 255, 258, 378
HapII CCGG 1 cut(s) 196
HhaI GCGC 1 cut(s) 377
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 1 cut(s) 207
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 29, 190
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 2 cut(s) 29, 190
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 2 cut(s) 177, 218
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 3 cut(s) 97, 299, 332
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 68
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 1 cut(s) 156
LpnPI CCDG 4 cut(s) 69, 198, 209, 334
Lsp1109I GCAGC 5 cut(s) 87, 109, 241, 244, 364
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 68
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MboII GAAGA 1 cut(s) 25
MlyI GAGTC 1 cut(s) 201
MnlI CCTC 1 cut(s) 13
MseI TTAA 3 cut(s) 42, 72, 189
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 1 cut(s) 196
MspR9I CCNGG 1 cut(s) 196
Mva1269I GAATGC 1 cut(s) 237
NciI CCSGG 1 cut(s) 196
NdeI CATATG 1 cut(s) 49
NdeII GATC 1 cut(s) 156
NlaIII CATG 1 cut(s) 94
PctI GAATGC 1 cut(s) 237
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 5 cut(s) 99, 102, 256, 259, 379
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SaqAI TTAA 3 cut(s) 42, 72, 189
SatI GCNGC 5 cut(s) 98, 101, 255, 258, 378
Sau3AI GATC 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 274
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 196
SetI ASST 6 cut(s) 71, 78, 102, 187, 279, 317
SfaNI GCATC 1 cut(s) 315
SinI GGWCC 1 cut(s) 274
SpeI ACTAGT 1 cut(s) 229
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 194
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 2 cut(s) 177, 218
TaiI ACGT 1 cut(s) 71
TaqII GACCGA 1 cut(s) 32
Tru1I TTAA 3 cut(s) 42, 72, 189
Tru9I TTAA 3 cut(s) 42, 72, 189
TseI GCWGC 5 cut(s) 97, 100, 254, 257, 377
TspDTI ATGAA 2 cut(s) 26, 344
VpaK11BI GGWCC 1 cut(s) 274
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.