Rw5G037300

Hydroxyacyl-thioester dehydratase type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009371
Physical Location & Seq
Forward (+)
65709537 .. 65710232
696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037300.1

Sequence Viewer

Length: 492 bp
ATGCTCACTCGGAATTTAGTCCTGGGCAGACTACCTTCTCTGAGAGGATTTTCATCAGCAGCAGCTACTTTTCTTAAAACTGGAGATATATTCAAACAAGCTAGAATTTTCACAAGTGAAGATGTCTTAGAGTACTCTAAAGTGAGTCATGACTCTAATCCTCTGCATTTTGATTCTGAGTCTGCTCGAAATGCTGGATTTGAAGACCGAGTGGTTCATGGGATGCTTGTTGCTGCACTGTTTCCCAAGATCATATCTTCTCATTTTCCTGGGGCTATATACATATTCCAAAGTCTGCATTTTAGGTTGCCGGTCTATATTGGAGAAGAGATAGTTGGTGAGGTACAAGCAAATAACATAAGAGAGCAAAAGAACAGATACTTAGCTAAATTCAAGACAGCATGCTATAAGGATGGTTCTGTTGTTATCACTGGTGAGGCTATGGCTATGTTACCTTCACTCGCTATGGAACAAGTGACTACTTCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00630 ko00650 ko01100 ko01110 ko01120 ko01130 ko01200 ko03008 map00230 map00630 map00650 map01100 map01110 map01120 map01130 map01200 map03008
Pfam Domains
Protein Families
MaoC_dehydratas

Protein Analysis

163

Amino Acids

18.05

Weight (kDa)

7.9

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MaoC_dehydratas PF01575 33 - 125 3.6e-19 MaoC like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 13, 105, 389
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 2 cut(s) 134, 345
AgsI TTSAA 3 cut(s) 94, 203, 394
AjnI CCWGG 2 cut(s) 21, 268
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 3 cut(s) 65, 101, 386
AluI AGCT 3 cut(s) 65, 101, 386
ApeKI GCWGC 3 cut(s) 59, 62, 233
ApoI RAATTY 3 cut(s) 13, 105, 389
AsuHPI GGTGA 2 cut(s) 350, 446
BbsI GAAGAC 1 cut(s) 210
BbvI GCAGC 3 cut(s) 71, 74, 220
BccI CCATC 1 cut(s) 407
BciT130I CCWGG 2 cut(s) 23, 270
BfaI CTAG 1 cut(s) 102
BisI GCNGC 3 cut(s) 60, 63, 234
BlsI GCNGC 3 cut(s) 61, 64, 235
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 2 cut(s) 23, 270
BmrFI CCNGG 2 cut(s) 23, 270
BmsI GCATC 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 22, 269
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 2 cut(s) 85, 436
Bse8I GATNNNNATC 1 cut(s) 52
BseBI CCWGG 2 cut(s) 23, 270
BseDI CCNNGG 2 cut(s) 22, 269
BseGI GGATG 2 cut(s) 228, 418
BseJI GATNNNNATC 1 cut(s) 52
BseMII CTCAG 2 cut(s) 32, 168
BseNI ACTGG 2 cut(s) 85, 436
BseXI GCAGC 3 cut(s) 71, 74, 220
BsgI GTGCAG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 311
Bsp143I GATC 1 cut(s) 249
BspCNI CTCAG 2 cut(s) 33, 169
BspHI TCATGA 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 2 cut(s) 85, 436
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 22, 269
BssMI GATC 1 cut(s) 249
Bst2UI CCWGG 2 cut(s) 23, 270
Bst4CI ACNGT 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 4 cut(s) 41, 127, 177, 382
BstF5I GGATG 2 cut(s) 228, 418
BstKTI GATC 1 cut(s) 252
BstMBI GATC 1 cut(s) 249
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 2 cut(s) 23, 270
BstNSI RCATGY 1 cut(s) 405
BstSCI CCNGG 2 cut(s) 21, 268
BstV1I GCAGC 3 cut(s) 71, 74, 220
BstV2I GAAGAC 1 cut(s) 210
BtsCI GGATG 2 cut(s) 228, 418
BtsIMutI CAGTG 2 cut(s) 236, 429
Cac8I GCNNGC 1 cut(s) 403
CciI TCATGA 1 cut(s) 148
Cfr10I RCCGGY 1 cut(s) 310
Csp6I GTAC 2 cut(s) 133, 344
CviAII CATG 3 cut(s) 149, 218, 402
CviJI RGCY 6 cut(s) 65, 101, 275, 386, 440, 446
CviKI_1 RGCY 6 cut(s) 65, 101, 275, 386, 440, 446
CviQI GTAC 2 cut(s) 133, 344
DdeI CTNAG 4 cut(s) 41, 127, 177, 382
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 468
EcoRII CCWGG 2 cut(s) 21, 268
FaeI CATG 3 cut(s) 152, 221, 405
FatI CATG 3 cut(s) 148, 217, 401
Fnu4HI GCNGC 3 cut(s) 60, 63, 234
FokI GGATG 2 cut(s) 235, 425
Fsp4HI GCNGC 3 cut(s) 60, 63, 234
FspBI CTAG 1 cut(s) 102
GluI GCNGC 3 cut(s) 60, 63, 234
GsuI CTGGAG 1 cut(s) 102
HapII CCGG 1 cut(s) 311
Hin1II CATG 3 cut(s) 152, 221, 405
HinfI GANTC 4 cut(s) 145, 152, 173, 179
HpaII CCGG 1 cut(s) 311
HphI GGTGA 2 cut(s) 350, 446
Hpy188I TCNGA 3 cut(s) 12, 42, 178
Hpy188III TCNNGA 3 cut(s) 149, 394, 486
HpyAV CCTTC 2 cut(s) 45, 465
HpyCH4III ACNGT 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 166, 236, 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 4 cut(s) 41, 127, 177, 382
Hsp92II CATG 3 cut(s) 152, 221, 405
Kzo9I GATC 1 cut(s) 249
LpnPI CCDG 9 cut(s) 8, 35, 66, 180, 255, 282, 324, 417, 471
Lsp1109I GCAGC 3 cut(s) 71, 74, 220
LweI GCATC 1 cut(s) 213
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 450, 475
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 4 cut(s) 131, 215, 249, 338
MluCI AATT 3 cut(s) 13, 105, 389
MlyI GAGTC 3 cut(s) 146, 154, 188
MnlI CCTC 4 cut(s) 38, 171, 334, 430
MseI TTAA 1 cut(s) 75
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 2 cut(s) 23, 270
MvaI CCWGG 2 cut(s) 23, 270
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeII GATC 1 cut(s) 249
NlaIII CATG 3 cut(s) 152, 221, 405
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 405
PaeI GCATGC 1 cut(s) 405
PagI TCATGA 1 cut(s) 148
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 3 cut(s) 61, 64, 235
PleI GAGTC 3 cut(s) 146, 153, 187
PpsI GAGTC 3 cut(s) 146, 153, 187
Psp6I CCWGG 2 cut(s) 21, 268
PspGI CCWGG 2 cut(s) 21, 268
RsaI GTAC 2 cut(s) 134, 345
RsaNI GTAC 2 cut(s) 133, 344
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 3 cut(s) 60, 63, 234
Sau3AI GATC 1 cut(s) 249
ScaI AGTACT 1 cut(s) 134
SchI GAGTC 3 cut(s) 146, 154, 188
ScrFI CCNGG 2 cut(s) 23, 270
SetI ASST 7 cut(s) 37, 67, 103, 308, 345, 388, 457
SfaNI GCATC 1 cut(s) 213
SphI GCATGC 1 cut(s) 405
Sse9I AATT 3 cut(s) 13, 105, 389
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 21, 268
TaaI ACNGT 1 cut(s) 240
TaqI TCGA 1 cut(s) 187
TaqII GACCGA 1 cut(s) 222
TasI AATT 3 cut(s) 13, 105, 389
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 2 cut(s) 243, 436
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 3 cut(s) 59, 62, 233
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 42, 206
TspRI CASTG 2 cut(s) 243, 436
XapI RAATTY 3 cut(s) 13, 105, 389
XceI RCATGY 1 cut(s) 405
XspI CTAG 1 cut(s) 102
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.