Rorug05G0332300

Hydroxyacyl-thioester dehydratase type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009371
Physical Location & Seq
Reverse (-)
40242438 .. 40242962
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0332300.1

Sequence Viewer

Length: 456 bp
ATGGGACTTCCGTGTTCTGGCCCGCCTCTAGATGTGGCTTTGAGGGAATCTATTACCCCGACGAGGAGAAATCTGGATCAGGTTATGGCTAGGGTTGATAACACCTCGGCGGTTGGCTCTTCCTTGGTGTTCGGAGCTCAGAGGGCGCTTCAACCCAGACCAGTAGGGGAGGGCTCTTCCGGTAGCAAGTTGGTACCTGAGGGTGATGGTCATGAGGTGCGGGGTTCCGAGCACCTGGAGAGTGAAGTCGAGGCTAATGTTGATGGTCTGGAGGCCAACGAGGATGGCGAGACTACCAAACCTTTGTCTGGCGAATTGGTTGTGGCCATGACGTCGCCAGTAGTTGGTTTGAGGAGGAAAAGATTGGAGGAAGATGGTGTCTCTCCAAAGAAACCAAGGTTCACTCTAGCTATGGGCAGAACCAATTTGGACAATGTTACTATGGGATTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00630 ko00650 ko01100 ko01110 ko01120 ko01130 ko01200 ko03008 map00230 map00630 map00650 map01100 map01110 map01120 map01130 map01200 map03008
Pfam Domains
Protein Families
MaoC_dehydratas

Protein Analysis

151

Amino Acids

16.03

Weight (kDa)

5.1

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 335
Acc65I GGTACC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 193
AccB7I CCANNNNNTGG 1 cut(s) 344
AciI CCGC 3 cut(s) 23, 110, 220
AclWI GGATC 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 324
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 4 cut(s) 17, 63, 308, 344
AgsI TTSAA 1 cut(s) 152
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 2 cut(s) 137, 410
AluI AGCT 2 cut(s) 137, 410
Alw21I GWGCWC 2 cut(s) 139, 234
Alw26I GTCTC 2 cut(s) 284, 385
AlwI GGATC 1 cut(s) 84
AoxI GGCC 3 cut(s) 19, 273, 324
Asp718I GGTACC 1 cut(s) 193
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 215
AxyI CCTNAGG 1 cut(s) 198
BalI TGGCCA 1 cut(s) 326
BanI GGYRCC 1 cut(s) 193
BanII GRGCYC 2 cut(s) 139, 176
Bbv12I GWGCWC 2 cut(s) 139, 234
BccI CCATC 4 cut(s) 200, 257, 278, 368
BciT130I CCWGG 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 284, 385
BfaI CTAG 3 cut(s) 29, 90, 407
BfoI RGCGCY 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 2 cut(s) 195, 226
BmrFI CCNGG 1 cut(s) 236
BpmI CTGGAG 2 cut(s) 257, 290
BsaHI GRCGYC 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 105, 123, 395
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 63, 308, 344
Bse1I ACTGG 2 cut(s) 161, 338
Bse21I CCTNAGG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 3 cut(s) 105, 123, 395
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 4 cut(s) 17, 63, 308, 344
BseMII CTCAG 2 cut(s) 152, 189
BseNI ACTGG 2 cut(s) 161, 338
BseRI GAGGAG 2 cut(s) 79, 367
BshFI GGCC 3 cut(s) 21, 275, 326
BshNI GGYRCC 1 cut(s) 193
BsiHKAI GWGCWC 2 cut(s) 139, 234
BsiSI CCGG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 4 cut(s) 17, 63, 308, 344
BsmAI GTCTC 2 cut(s) 284, 385
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 3 cut(s) 21, 275, 326
Bsp1286I GDGCHC 3 cut(s) 139, 176, 234
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 3 cut(s) 23, 110, 220
BspANI GGCC 3 cut(s) 21, 275, 326
BspCNI CTCAG 2 cut(s) 151, 190
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 195, 226
BspPI GGATC 1 cut(s) 84
BspQI GCTCTTC 2 cut(s) 124, 181
BspT107I GGYRCC 1 cut(s) 193
BsrI ACTGG 2 cut(s) 161, 338
BssECI CCNNGG 3 cut(s) 105, 123, 395
BssMI GATC 1 cut(s) 76
BssNI GRCGYC 1 cut(s) 332
BssT1I CCWWGG 2 cut(s) 123, 395
Bst2UI CCWGG 1 cut(s) 236
Bst6I CTCTTC 2 cut(s) 124, 181
BstACI GRCGYC 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 138, 198
BstF5I GGATG 1 cut(s) 289
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 2 cut(s) 284, 385
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 234
Bsu36I CCTNAGG 1 cut(s) 198
BsuRI GGCC 3 cut(s) 21, 275, 326
BtsCI GGATG 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 23
CciI TCATGA 1 cut(s) 211
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 20
Csp6I GTAC 1 cut(s) 194
CviAII CATG 2 cut(s) 212, 328
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 2 cut(s) 138, 198
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 2 cut(s) 124, 181
EarI CTCTTC 2 cut(s) 124, 181
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 2 cut(s) 123, 395
Eco24I GRGCYC 2 cut(s) 139, 176
Eco53kI GAGCTC 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 198
EcoICRI GAGCTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 2 cut(s) 123, 395
EcoT38I GRGCYC 2 cut(s) 139, 176
ErhI CCWWGG 2 cut(s) 123, 395
FaeI CATG 2 cut(s) 215, 331
FaiI YATR 5 cut(s) 86, 213, 329, 413, 443
FaqI GGGAC 1 cut(s) 18
FatI CATG 2 cut(s) 211, 327
FauI CCCGC 2 cut(s) 30, 213
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 2 cut(s) 139, 176
FspBI CTAG 3 cut(s) 29, 90, 407
GlaI GCGC 1 cut(s) 147
GsuI CTGGAG 2 cut(s) 257, 290
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 3 cut(s) 21, 275, 326
HapII CCGG 1 cut(s) 180
HhaI GCGC 1 cut(s) 148
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 2 cut(s) 215, 331
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HinfI GANTC 1 cut(s) 47
HpaII CCGG 1 cut(s) 180
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 402
Hpy188I TCNGA 3 cut(s) 134, 141, 229
Hpy188III TCNNGA 4 cut(s) 29, 74, 212, 269
Hpy8I GTNNAC 1 cut(s) 402
Hpy99I CGWCG 2 cut(s) 64, 337
HpyCH4IV ACGT 1 cut(s) 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 2 cut(s) 138, 198
HpySE526I ACGT 1 cut(s) 332
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 2 cut(s) 215, 331
HspAI GCGC 1 cut(s) 146
KpnI GGTACC 1 cut(s) 197
Kzo9I GATC 1 cut(s) 76
LguI GCTCTTC 2 cut(s) 124, 181
LmnI GCTCC 1 cut(s) 134
MaeI CTAG 3 cut(s) 29, 90, 407
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 436
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 3 cut(s) 111, 168, 383
MhlI GDGCHC 3 cut(s) 139, 176, 234
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 2 cut(s) 314, 424
MluNI TGGCCA 1 cut(s) 326
Mox20I TGGCCA 1 cut(s) 326
MscI TGGCCA 1 cut(s) 326
Msp20I TGGCCA 1 cut(s) 326
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 143
NdeII GATC 1 cut(s) 76
NlaIII CATG 2 cut(s) 215, 331
NlaIV GGNNCC 2 cut(s) 195, 226
NmeAIII GCCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 211
PciSI GCTCTTC 2 cut(s) 124, 181
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 195, 226
PspPI GGNCC 1 cut(s) 20
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
SacI GAGCTC 1 cut(s) 139
SapI GCTCTTC 2 cut(s) 124, 181
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 3 cut(s) 139, 176, 234
Sse9I AATT 2 cut(s) 314, 424
SsiI CCGC 3 cut(s) 23, 110, 220
SspMI CTAG 3 cut(s) 29, 90, 407
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 2 cut(s) 123, 395
TaiI ACGT 1 cut(s) 335
TaqI TCGA 1 cut(s) 249
TasI AATT 2 cut(s) 314, 424
TfiI GAWTC 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 344
XbaI TCTAGA 1 cut(s) 28
XspI CTAG 3 cut(s) 29, 90, 407
ZraI GACGTC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.