Rorug05G0332200

Hydroxyacyl-thioester dehydratase type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009371
Physical Location & Seq
Forward (+)
40209646 .. 40211056
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0332200.1

Sequence Viewer

Length: 645 bp
ATGTATCTCGTATGGCGTATGAAGACACAGTGGGATTTAGAGGTAGGTGGAGATGGGAATGGGGGTCTGAATCGGTTTATCCCGGCCTCGACTCTAAAGGCCCGTGCTCAAAGGGCACTCCATCCCAGACCAGCAGGGGAGGGCTCTTCCGGTAGCAAGTTGGTACCTGAGGGTGATGGTCAGGAGGTGCGGGGTTCCGAGCACCCGAAGAGTGAAGTCGAGGCTAATGCTGATAGTCTGGAGGACAACGAGGATGGCGAGACTAACGAACCTTCATTTGGTGAATTGGTTGTGGCCATGAAATCGCCAGCAGTTGGTTTGAGGAGGAAAAGATTAATTAGAGGAAGATGGTGTCTCTCCAAAGAAACCAAGGCTCAGTCTAGCTGTGGGCAGAACCAATTTGGACAATGTTACTATGGGATTGTTTTGAATTCTTCTCAGGAGAGTACTAAGAAGCGGGGTCGTCCTCGAGGTGCAAAAAACAAGGTGAAAATTGACTGCAAGGTGGTGAAAACCTCACTTATGAAGAAAAGCAAGGCTTCAAAGAAGATAGAAAAAATGGTGAATGGCCTCCCGGAGTTTACTTTGCCAAATGAGAAAGGTACACCCAAAGCTTCCAAGGGTAAGGAGGTGATAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00630 ko00650 ko01100 ko01110 ko01120 ko01130 ko01200 ko03008 map00230 map00630 map00650 map01100 map01110 map01120 map01130 map01200 map03008
Pfam Domains
Protein Families
MaoC_dehydratas

Protein Analysis

214

Amino Acids

23.39

Weight (kDa)

9.72

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 468
Acc65I GGTACC 1 cut(s) 163
AccB1I GGYRCC 1 cut(s) 163
AccB7I CCANNNNNTGG 1 cut(s) 314
AciI CCGC 2 cut(s) 190, 457
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 1 cut(s) 430
AfaI GTAC 3 cut(s) 165, 448, 604
AfiI CCNNNNNNNGG 2 cut(s) 278, 314
AgsI TTSAA 2 cut(s) 430, 543
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
AluBI AGCT 2 cut(s) 384, 614
AluI AGCT 2 cut(s) 384, 614
Alw21I GWGCWC 2 cut(s) 109, 204
Alw26I GTCTC 2 cut(s) 254, 359
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 4 cut(s) 84, 99, 294, 568
ApoI RAATTY 1 cut(s) 430
AseI ATTAAT 1 cut(s) 335
Asp718I GGTACC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 100
AsuC2I CCSGG 2 cut(s) 83, 575
AsuHPI GGTGA 6 cut(s) 185, 293, 499, 520, 574, 643
AvaI CYCGRG 1 cut(s) 468
AxyI CCTNAGG 1 cut(s) 168
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 296
BanI GGYRCC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 29
Bbv12I GWGCWC 2 cut(s) 109, 204
BccI CCATC 5 cut(s) 47, 129, 170, 248, 342
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BcnI CCSGG 2 cut(s) 83, 575
BcoDI GTCTC 2 cut(s) 254, 359
BfaI CTAG 1 cut(s) 381
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 83, 575
BmeT110I CYCGRG 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 165, 196
BmrFI CCNGG 2 cut(s) 83, 575
BpiI GAAGAC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 260
BpuMI CCSGG 2 cut(s) 83, 575
BsaJI CCNNGG 2 cut(s) 369, 618
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 314
Bse21I CCTNAGG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 369, 618
BseGI GGATG 2 cut(s) 121, 259
BseLI CCNNNNNNNGG 2 cut(s) 278, 314
BseMII CTCAG 3 cut(s) 159, 389, 452
BseRI GAGGAG 1 cut(s) 337
BseSI GKGCMC 1 cut(s) 118
BshFI GGCC 4 cut(s) 86, 101, 296, 570
BshNI GGYRCC 1 cut(s) 163
BsiHKAI GWGCWC 2 cut(s) 109, 204
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 3 cut(s) 83, 150, 575
BslI CCNNNNNNNGG 2 cut(s) 278, 314
BsmAI GTCTC 2 cut(s) 254, 359
BsnI GGCC 4 cut(s) 86, 101, 296, 570
BsoBI CYCGRG 1 cut(s) 468
Bsp1286I GDGCHC 4 cut(s) 109, 118, 146, 204
BspACI CCGC 2 cut(s) 190, 457
BspANI GGCC 4 cut(s) 86, 101, 296, 570
BspCNI CTCAG 3 cut(s) 160, 388, 451
BspLI GGNNCC 2 cut(s) 165, 196
BspQI GCTCTTC 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 163
BssECI CCNNGG 2 cut(s) 369, 618
BssT1I CCWWGG 2 cut(s) 369, 618
Bst4CI ACNGT 1 cut(s) 30
Bst6I CTCTTC 2 cut(s) 151, 203
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 4 cut(s) 168, 375, 438, 450
BstF5I GGATG 2 cut(s) 121, 259
BstMAI GTCTC 2 cut(s) 254, 359
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 81, 573
BstSLI GKGCMC 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 29
Bsu36I CCTNAGG 1 cut(s) 168
BsuRI GGCC 4 cut(s) 86, 101, 296, 570
BtsCI GGATG 2 cut(s) 121, 259
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 100
Csp6I GTAC 3 cut(s) 164, 447, 603
CviAII CATG 1 cut(s) 298
CviQI GTAC 3 cut(s) 164, 447, 603
DdeI CTNAG 4 cut(s) 168, 375, 438, 450
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 151, 203
EarI CTCTTC 2 cut(s) 151, 203
Eco130I CCWWGG 2 cut(s) 369, 618
Eco24I GRGCYC 1 cut(s) 146
Eco81I CCTNAGG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 468
EcoRI GAATTC 1 cut(s) 430
EcoT14I CCWWGG 2 cut(s) 369, 618
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 369, 618
FaeI CATG 1 cut(s) 301
FaiI YATR 5 cut(s) 13, 20, 299, 417, 524
FalI AAGNNNNNCTT 2 cut(s) 523, 555
FatI CATG 1 cut(s) 297
FauI CCCGC 2 cut(s) 183, 450
FokI GGATG 2 cut(s) 108, 266
FriOI GRGCYC 1 cut(s) 146
FspBI CTAG 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 260
HaeIII GGCC 4 cut(s) 86, 101, 296, 570
HapII CCGG 3 cut(s) 83, 150, 575
Hin1II CATG 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 612
HinfI GANTC 2 cut(s) 70, 91
HpaII CCGG 3 cut(s) 83, 150, 575
HphI GGTGA 6 cut(s) 185, 293, 499, 520, 574, 643
Hpy166II GTNNAC 2 cut(s) 582, 605
Hpy188I TCNGA 2 cut(s) 69, 199
Hpy188III TCNNGA 3 cut(s) 182, 239, 440
Hpy8I GTNNAC 2 cut(s) 582, 605
HpyAV CCTTC 1 cut(s) 282
HpyCH4III ACNGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 476, 501
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 4 cut(s) 168, 375, 438, 450
Hsp92II CATG 1 cut(s) 301
KpnI GGTACC 1 cut(s) 167
LguI GCTCTTC 1 cut(s) 151
MaeI CTAG 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 410
MboII GAAGA 7 cut(s) 34, 138, 220, 357, 426, 538, 559
MhlI GDGCHC 4 cut(s) 109, 118, 146, 204
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 5 cut(s) 284, 336, 398, 430, 492
MluNI TGGCCA 1 cut(s) 296
MlyI GAGTC 1 cut(s) 85
Mox20I TGGCCA 1 cut(s) 296
MscI TGGCCA 1 cut(s) 296
MseI TTAA 1 cut(s) 335
Msp20I TGGCCA 1 cut(s) 296
MspI CCGG 3 cut(s) 83, 150, 575
MspR9I CCNGG 2 cut(s) 83, 575
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 2 cut(s) 83, 575
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 2 cut(s) 165, 196
PaeR7I CTCGAG 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 1 cut(s) 70
PflMI CCANNNNNTGG 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 573
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PshBI ATTAAT 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 165, 196
PspPI GGNCC 1 cut(s) 100
PspXI VCTCGAGB 1 cut(s) 468
RsaI GTAC 3 cut(s) 165, 448, 604
RsaNI GTAC 3 cut(s) 164, 447, 603
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 1 cut(s) 335
Sau96I GGNCC 1 cut(s) 100
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 1 cut(s) 85
ScrFI CCNGG 2 cut(s) 83, 575
SduI GDGCHC 4 cut(s) 109, 118, 146, 204
Sfr274I CTCGAG 1 cut(s) 468
SlaI CTCGAG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
Sse9I AATT 5 cut(s) 284, 336, 398, 430, 492
SsiI CCGC 2 cut(s) 190, 457
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 2 cut(s) 81, 573
StyI CCWWGG 2 cut(s) 369, 618
TaaI ACNGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 89, 219, 469
TasI AATT 5 cut(s) 284, 336, 398, 430, 492
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 1 cut(s) 35
TspDTI ATGAA 4 cut(s) 35, 264, 314, 539
TspRI CASTG 1 cut(s) 35
Van91I CCANNNNNTGG 1 cut(s) 314
VspI ATTAAT 1 cut(s) 335
XapI RAATTY 1 cut(s) 430
XhoI CTCGAG 1 cut(s) 468
XspI CTAG 1 cut(s) 381
ZrmI AGTACT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.