Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0007476

Physical Location & Seq

Genomic Coordinates Forward (+)

42598285 .. 42609654

Gene Structure 11370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0345800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGAGAGCGCTGGTCTCTCTGAAGATCTCTCCGATTCCACTCAGCGTCCCGACTCCGACAGCGAGCAGCGAGTTTACTTTGTTCCCTACAGTTGGTGGAGGGATGCACAAGATTCAGTTGTGGGCGATTCAGATGGGAACAAAGGGGTTTTGTGTGTGGCATCCCCGGCCGCTTCGTCTTATGGTGGCCCTATGAAGATCATCAACAACATTTTCAACTCAGATCTCGTCTTCAATCTCAGGAGAGAGGATGAGGCTGTGCACAATGCTGAAAATGCCGAGGTCGGTGTATCGGGCCGGGACTATGCATTGGTCCCCGGAGAAATGTGGGTTCAGGCGCTCAGATGGCATAGTGATCCTAAAGCTGCAGTTAAGGATGTTAGGAGTTTTTCGGCTGCAGAAGATGACAAGACCGATGTCTATCCCCTACAACTGAGGCTTTCTGTTCCACGAGAAACAAATTCATTGGGCGTAAGAATTACCAAAAAGGATAATGCAGTTGAGTCCTTCAAAAGAGCCTGCAAAATCTTCAGTTTGGAGTCTGACTTGTTACGCATCTGGGACTTTTCCGGCCAGACTAACTTATTTTTTACAAATGACAAAACCAAGTTTCCAAGAGACTTGCAACGGCAGTCAGATGAGATCCTACTGGAGTTGCAAGTTTATGGATTGTCAGATTCCATCAAGTGCAGGGAAGGGAAAAAAGATGAGATGGCCATGCAACATTCTACCATTGGAAGTTCTTGTTCTGGTTCTTCAGTTATGATGAATGGTTCTGCTGGCAATATGCATTCCAATTTCATTCGTTCTTTCTGGGGAAGCTCTTGTGAAGCTGGTTCTTTAGGGTTGACAGGATTGCAAAACCTCGGAAATACTTGTTTCATGAATAGCTCCATCCAGTGCTTAGCTCATACACCAAAGCTTGTTGACTATTTTCTTGGAGATTATGCTAGAGAAATAAATCAGGAAAACCCATTGGGCATGGATGGGGAGATTGCTTTAGCATTTGGAGATCTGTTAAGGAAGTTATGGGCTCCTGGAGCAACTCCTGTGCCTCCAAGGACCTTTAAATCAAAACTTGCTCGTTTTGCTCCTCAGTTTAATGGCTTTAATCAACATGATTCACAGGAACTCCTGGCCTTTTTGTTGGATGGACTTCATGAAGATCTTAATCGTGTAAAATGCAAGCCTTACGTAGAAGTCAAGGATGGTGATGATCGACCGGATGAAGAGGTAGCTGATGAATATTGGCGAAATCATTTAGCCCGCAATGACTCTATCATTGTTGATGTGTGCCAAGGTCAATATAAATCAACATTAGTTTGTCCTGTTTGCAAAAAGGTCTCTGTTACATTTGATCCGTTTATGTACCTGTCACTACCATTACCTTCAACAATGATGCGGACAATGACTTTAACAGTTATCAACACTGATGGAAGTTCGAAGCCATCTGAATATACCATTAATGTGCCCAAGCATGGAAAGTTTGACGATCTCATCCGCAGTTTGAGTGTTGCATGCTCACTTGGGGTTGATGAAACCTTTTTAGTGGCCGAGATATACAACAATCGCATTATACGTTATCTCGAGGAGCCTGCTGATTCATTATCCTTAATTAGAGATGGTGACCAGTTGGTTGCTTATCGGTTTATGAAAGATGTTGAGGAAGTACCTCTGGTAGTTTTCATGCATCAACAAATGGAGCAGGACATGCATGGGAAGTTGAGTTCAAGCTGGAAACCGTTTGGAATTCCTCTTGTATCAAGACTTCGTACTTCGGCAACTGGATCTGATATCTGCTATCAGTACTTAAACTTAGTTAAGCCATTTCAAATACCTGGAAAAGATTCCCAAGCACTTGTTGATAATTCACAGGGCACTGCTCCTATAGAGGCCACTGGAGCAGAGGGTACCGCACCTCCTGTTTCAGGTGGGGATGTGAACCTCTGTTATGAAAATGGGGCTGACTCACCATTGGATTGTGAATTTCAGTTTTATATAACAGATGAAAAGGGTATTCAAAAGGAGAAAAAGATAGTAATGAATGAGATAGTCGAGAGGAAAATGTCGGAGCATTTATATGTTGCAGTATGTTGGCCAGAAAAATACGTTGAGCAGTATGATACTCGCGTTCTGAGCTCATTGCCGTTGGTTTTCAAGCCTGGCCTTTTTTCTAAAAAGCCTCAGGAGACTGTTTCTCTATATAAGTGCCTTGAAGCATTCTTGAAGGAGGAGCCTCTTGGGCCAGAAGACATGTGGTCAGTGGGTATTGTCCTGGCTGCAAAAAGCATTGCCAGGCCAGTAAGAAGTTGGATCTCTGGAGACTGCCTGAGATCTTGGTCATTCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030957 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046983 GO:0048285 GO:0050896 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

783

Amino Acids

87.54

Weight (kDa)

5.05

Isoelectric Point (pI)

41.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	145 - 199	3.9e-30	UBP8-like, ubiquitin-like domain
UCH	PF00443	284 - 566	3.7e-37	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007476)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02062 FvH4_7g23893
rosa_chinensis	RchiOBHm_Chr3g0447681 RchiOBHm_Chr5g0062071
rosa_laevigata	RLG00000015854 RLG00000025979 RLG00000035529
rosa_multiflora	Rmu_sc0002296.1_g000012 Rmu_sc0006064.1_g000010 Rmu_sc0017669.1_g000013
rosa_roxburghii	Rroxscaffold_2G00153710
rosa_rugosa	Rorug01G0475300 Rorug05G0345800
rosa_samantha	Rh2BG027000 Rh2BG028800 Rh3AG004200 Rh3BG003800 Rh3CG004200 Rh3DG004100 Rh5AG407400 Rh5BG420600 Rh5BG420700 Rh5CG444600 Rh5CG444800 Rh5DG435800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1910
AccB1I	GGYRCC	1 cut(s)	1910
AccII	CGCG	1 cut(s)	2130
AciI	CCGC	5 cut(s)	171, 1267, 1402, 1501, 1914
AclWI	GGATC	5 cut(s)	350, 637, 1352, 1795, 2321
AcoI	YGGCCR	5 cut(s)	168, 571, 714, 1551, 2096
AcsI	RAATTY	3 cut(s)	460, 1749, 1985
AcuI	CTGAAG	3 cut(s)	42, 514, 741
AfaI	GTAC	5 cut(s)	1370, 1671, 1774, 1808, 1912
AfeI	AGCGCT	1 cut(s)	10
AfiI	CCNNNNNNNGG	3 cut(s)	93, 1478, 1928
AflIII	ACRYGT	1 cut(s)	2253
AhdI	GACNNNNNGTC	1 cut(s)	2257
AjnI	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2274, 2294
AloI	GAACNNNNNNTCC	2 cut(s)	1711, 1743
AluBI	AGCT	9 cut(s)	365, 822, 833, 891, 908, 922, 1238, 1734, 2139
AluI	AGCT	9 cut(s)	365, 822, 833, 891, 908, 922, 1238, 1734, 2139
Alw21I	GWGCWC	2 cut(s)	264, 2141
Alw26I	GTCTC	5 cut(s)	20, 612, 1348, 2183, 2316
Alw44I	GTGCAC	1 cut(s)	260
AlwI	GGATC	5 cut(s)	350, 637, 1352, 1795, 2321
Ama87I	CYCGRG	1 cut(s)	1586
Aor51HI	AGCGCT	1 cut(s)	10
ApaLI	GTGCAC	1 cut(s)	260
ApeKI	GCWGC	4 cut(s)	67, 365, 395, 2279
ApoI	RAATTY	3 cut(s)	460, 1749, 1985
AseI	ATTAAT	1 cut(s)	1464
Asp700I	GAANNNNTTC	1 cut(s)	1846
Asp718I	GGTACC	1 cut(s)	1910
AspLEI	GCGC	2 cut(s)	11, 340
AspS9I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
AsuC2I	CCSGG	3 cut(s)	167, 299, 318
AsuHPI	GGTGA	3 cut(s)	1223, 1637, 1962
AsuII	TTCGAA	1 cut(s)	1442
AvaI	CYCGRG	1 cut(s)	1586
AvaII	GGWCC	2 cut(s)	313, 1062
AxyI	CCTNAGG	1 cut(s)	2184
BaeGI	GKGCMC	3 cut(s)	264, 1473, 1880
BaeI	ACNNNNGTAYC	2 cut(s)	2115, 2148
BalI	TGGCCA	2 cut(s)	716, 2098
BanI	GGYRCC	1 cut(s)	1910
BanII	GRGCYC	2 cut(s)	1036, 2141
BarI	GAAGNNNNNNTAC	2 cut(s)	1752, 1784
BauI	CACGAG	1 cut(s)	450
BbsI	GAAGAC	2 cut(s)	223, 2256
Bbv12I	GWGCWC	2 cut(s)	264, 2141
BbvI	GCAGC	4 cut(s)	79, 352, 382, 2266
BceAI	ACGGC	2 cut(s)	644, 2131
BcgI	CGANNNNNNTGC	2 cut(s)	1577, 1611
BciT130I	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2276, 2296
BcnI	CCSGG	3 cut(s)	167, 299, 318
BcoDI	GTCTC	5 cut(s)	20, 612, 1348, 2183, 2316
BfaI	CTAG	1 cut(s)	951
BfmI	CTRYAG	4 cut(s)	88, 366, 396, 1887
BfoI	RGCGCY	2 cut(s)	12, 341
BglI	GCCNNNNNGGC	1 cut(s)	2242
BglII	AGATCT	5 cut(s)	25, 223, 1012, 1165, 2333
BisI	GCNGC	5 cut(s)	68, 171, 366, 396, 2280
BlpI	GCTNAGC	1 cut(s)	904
BlsI	GCNGC	5 cut(s)	69, 172, 367, 397, 2281
BmcAI	AGTACT	1 cut(s)	1808
Bme1390I	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2276, 2296
Bme18I	GGWCC	2 cut(s)	313, 1062
BmeRI	GACNNNNNGTC	1 cut(s)	2257
BmeT110I	CYCGRG	1 cut(s)	1586
BmgT120I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
BmiI	GGNNCC	5 cut(s)	315, 1035, 1593, 1912, 2235
BmrFI	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2276, 2296
BmsI	GCATC	5 cut(s)	94, 170, 564, 1389, 1699
BoxI	GACNNNNGTC	1 cut(s)	416
BpiI	GAAGAC	2 cut(s)	223, 2256
BpmI	CTGGAG	4 cut(s)	671, 1059, 1920, 2340
Bpu1102I	GCTNAGC	1 cut(s)	904
Bpu14I	TTCGAA	1 cut(s)	1442
BpuMI	CCSGG	3 cut(s)	167, 299, 318
BsaAI	YACGTR	1 cut(s)	1195
BsaBI	GATNNNNATC	2 cut(s)	420, 1170
BsaI	GGTCTC	2 cut(s)	20, 1348
BsaJI	CCNNGG	6 cut(s)	165, 279, 316, 865, 1058, 1297
BsaWI	WCCGGW	1 cut(s)	1222
BsaXI	ACNNNNNCTCC	4 cut(s)	1116, 1146, 1903, 1933
Bsc4I	CCNNNNNNNGG	3 cut(s)	93, 1478, 1928
Bse1I	ACTGG	6 cut(s)	654, 898, 1630, 1789, 1903, 2300
Bse21I	CCTNAGG	1 cut(s)	2184
Bse3DI	GCAATG	3 cut(s)	1276, 2141, 2289
Bse8I	GATNNNNATC	2 cut(s)	420, 1170
BseBI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2276, 2296
BseDI	CCNNGG	6 cut(s)	165, 279, 316, 865, 1058, 1297
BseJI	GATNNNNATC	2 cut(s)	420, 1170
BseLI	CCNNNNNNNGG	3 cut(s)	93, 1478, 1928
BseMI	GCAATG	3 cut(s)	1276, 2141, 2289
BseMII	CTCAG	9 cut(s)	56, 234, 253, 355, 425, 1109, 2126, 2198, 2321
BseNI	ACTGG	6 cut(s)	654, 898, 1630, 1789, 1903, 2300
BseRI	GAGGAG	3 cut(s)	1083, 1604, 2246
BseSI	GKGCMC	3 cut(s)	264, 1473, 1880
BseX3I	CGGCCG	1 cut(s)	168
BseXI	GCAGC	4 cut(s)	79, 352, 382, 2266
BsgI	GTGCAG	1 cut(s)	709
Bsh1236I	CGCG	1 cut(s)	2130
Bsh1285I	CGRYCG	2 cut(s)	171, 1223
BshNI	GGYRCC	1 cut(s)	1910
BsiEI	CGRYCG	2 cut(s)	171, 1223
BsiHKAI	GWGCWC	2 cut(s)	264, 2141
BsiHKCI	CYCGRG	1 cut(s)	1586
BsiSI	CCGG	5 cut(s)	167, 298, 318, 570, 1223
BslFI	GGGAC	4 cut(s)	33, 299, 314, 575
BslI	CCNNNNNNNGG	3 cut(s)	93, 1478, 1928
BsmAI	GTCTC	5 cut(s)	20, 612, 1348, 2183, 2316
BsmFI	GGGAC	4 cut(s)	33, 299, 314, 575
BsmI	GAATGC	2 cut(s)	790, 2219
Bso31I	GGTCTC	2 cut(s)	20, 1348
BsoBI	CYCGRG	1 cut(s)	1586
Bsp119I	TTCGAA	1 cut(s)	1442
Bsp1286I	GDGCHC	5 cut(s)	264, 1036, 1473, 1880, 2141
Bsp1720I	GCTNAGC	1 cut(s)	904
BspACI	CCGC	5 cut(s)	171, 1267, 1402, 1501, 1914
BspCNI	CTCAG	9 cut(s)	55, 233, 252, 354, 426, 1108, 2127, 2197, 2322
BspFNI	CGCG	1 cut(s)	2130
BspHI	TCATGA	2 cut(s)	882, 1159
BspLI	GGNNCC	5 cut(s)	315, 1035, 1593, 1912, 2235
BspMAI	CTGCAG	2 cut(s)	370, 400
BspPI	GGATC	5 cut(s)	350, 637, 1352, 1795, 2321
BspT104I	TTCGAA	1 cut(s)	1442
BspT107I	GGYRCC	1 cut(s)	1910
BspTNI	GGTCTC	2 cut(s)	20, 1348
BsrDI	GCAATG	3 cut(s)	1276, 2141, 2289
BsrI	ACTGG	6 cut(s)	654, 898, 1630, 1789, 1903, 2300
BssECI	CCNNGG	6 cut(s)	165, 279, 316, 865, 1058, 1297
BssSI	CACGAG	1 cut(s)	450
BssT1I	CCWWGG	2 cut(s)	1058, 1297
Bst2BI	CACGAG	1 cut(s)	450
Bst2UI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2276, 2296
Bst4CI	ACNGT	4 cut(s)	92, 1420, 1743, 2194
Bst6I	CTCTTC	1 cut(s)	1224
BstAPI	GCANNNNNTGC	1 cut(s)	1711
BstBAI	YACGTR	1 cut(s)	1195
BstBI	TTCGAA	1 cut(s)	1442
BstC8I	GCNNGC	7 cut(s)	65, 520, 781, 1187, 1267, 1519, 1596
BstEII	GGTNACC	1 cut(s)	1625
BstENI	CCTNNNNNAGG	1 cut(s)	1926
BstFNI	CGCG	1 cut(s)	2130
BstH2I	RGCGCY	2 cut(s)	12, 341
BstHHI	GCGC	2 cut(s)	11, 340
BstMAI	GTCTC	5 cut(s)	20, 612, 1348, 2183, 2316
BstMCI	CGRYCG	2 cut(s)	171, 1223
BstMWI	GCNNNNNNNGC	9 cut(s)	167, 275, 346, 1040, 1088, 1711, 1901, 2136, 2242
BstNI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2276, 2296
BstNSI	RCATGY	3 cut(s)	1521, 1714, 2257
BstPAI	GACNNNNGTC	1 cut(s)	416
BstPI	GGTNACC	1 cut(s)	1625
BstSCI	CCNGG	9 cut(s)	165, 297, 316, 1036, 1134, 1837, 2161, 2274, 2294
BstSFI	CTRYAG	4 cut(s)	88, 366, 396, 1887
BstSLI	GKGCMC	3 cut(s)	264, 1473, 1880
BstSNI	TACGTA	1 cut(s)	1195
BstUI	CGCG	1 cut(s)	2130
BstV1I	GCAGC	4 cut(s)	79, 352, 382, 2266
BstV2I	GAAGAC	2 cut(s)	223, 2256
BstX2I	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2313, 2333
BstYI	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2313, 2333
BstZI	CGGCCG	1 cut(s)	168
Bsu36I	CCTNAGG	1 cut(s)	2184
BtsI	GCAGTG	1 cut(s)	1878
BtsIMutI	CAGTG	5 cut(s)	905, 1428, 1878, 1896, 2268
Cac8I	GCNNGC	7 cut(s)	65, 520, 781, 1187, 1267, 1519, 1596
CciI	TCATGA	2 cut(s)	882, 1159
CfoI	GCGC	2 cut(s)	11, 340
Cfr13I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
CseI	GACGC	1 cut(s)	35
Csp6I	GTAC	5 cut(s)	1369, 1670, 1773, 1807, 1911
CviQI	GTAC	5 cut(s)	1369, 1670, 1773, 1807, 1911
DraI	TTTAAA	1 cut(s)	1069
DriI	GACNNNNNGTC	1 cut(s)	2257
EaeI	YGGCCR	5 cut(s)	168, 571, 714, 1551, 2096
EagI	CGGCCG	1 cut(s)	168
Eam1104I	CTCTTC	1 cut(s)	1224
Eam1105I	GACNNNNNGTC	1 cut(s)	2257
EarI	CTCTTC	1 cut(s)	1224
Ecl136II	GAGCTC	1 cut(s)	2139
EclXI	CGGCCG	1 cut(s)	168
Eco105I	TACGTA	1 cut(s)	1195
Eco130I	CCWWGG	2 cut(s)	1058, 1297
Eco24I	GRGCYC	2 cut(s)	1036, 2141
Eco31I	GGTCTC	2 cut(s)	20, 1348
Eco32I	GATATC	1 cut(s)	1795
Eco47I	GGWCC	2 cut(s)	313, 1062
Eco47III	AGCGCT	1 cut(s)	10
Eco52I	CGGCCG	1 cut(s)	168
Eco53kI	GAGCTC	1 cut(s)	2139
Eco57I	CTGAAG	3 cut(s)	42, 514, 741
Eco81I	CCTNAGG	1 cut(s)	2184
Eco88I	CYCGRG	1 cut(s)	1586
Eco91I	GGTNACC	1 cut(s)	1625
EcoICRI	GAGCTC	1 cut(s)	2139
EcoNI	CCTNNNNNAGG	1 cut(s)	1926
EcoO109I	RGGNCCY	1 cut(s)	1062
EcoO65I	GGTNACC	1 cut(s)	1625
EcoRI	GAATTC	1 cut(s)	1749
EcoRII	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2274, 2294
EcoRV	GATATC	1 cut(s)	1795
EcoT14I	CCWWGG	2 cut(s)	1058, 1297
EcoT22I	ATGCAT	4 cut(s)	310, 792, 1692, 1716
EcoT38I	GRGCYC	2 cut(s)	1036, 2141
ErhI	CCWWGG	2 cut(s)	1058, 1297
FaqI	GGGAC	4 cut(s)	33, 299, 314, 575
FauI	CCCGC	1 cut(s)	1274
Fnu4HI	GCNGC	5 cut(s)	68, 171, 366, 396, 2280
FriOI	GRGCYC	2 cut(s)	1036, 2141
Fsp4HI	GCNGC	5 cut(s)	68, 171, 366, 396, 2280
FspBI	CTAG	1 cut(s)	951
GlaI	GCGC	2 cut(s)	10, 339
GluI	GCNGC	5 cut(s)	68, 171, 366, 396, 2280
GsuI	CTGGAG	4 cut(s)	671, 1059, 1920, 2340
HaeII	RGCGCY	2 cut(s)	12, 341
HapII	CCGG	5 cut(s)	167, 298, 318, 570, 1223
HgaI	GACGC	1 cut(s)	35
HhaI	GCGC	2 cut(s)	11, 340
Hin6I	GCGC	2 cut(s)	9, 338
HinP1I	GCGC	2 cut(s)	9, 338
HincII	GTYRAC	2 cut(s)	849, 928
HindII	GTYRAC	2 cut(s)	849, 928
HindIII	AAGCTT	1 cut(s)	920
HpaII	CCGG	5 cut(s)	167, 298, 318, 570, 1223
HphI	GGTGA	3 cut(s)	1223, 1637, 1962
Hpy166II	GTNNAC	5 cut(s)	76, 262, 849, 928, 1942
Hpy8I	GTNNAC	5 cut(s)	76, 262, 849, 928, 1942
HpyAV	CCTTC	4 cut(s)	517, 689, 1398, 2221
HpyCH4III	ACNGT	4 cut(s)	92, 1420, 1743, 2194
HpyCH4IV	ACGT	3 cut(s)	1194, 1579, 2109
HpyF10VI	GCNNNNNNNGC	9 cut(s)	167, 275, 346, 1040, 1088, 1711, 1901, 2136, 2242
HpySE526I	ACGT	3 cut(s)	1194, 1579, 2109
HspAI	GCGC	2 cut(s)	9, 338
KpnI	GGTACC	1 cut(s)	1914
Lsp1109I	GCAGC	4 cut(s)	79, 352, 382, 2266
LweI	GCATC	5 cut(s)	94, 170, 564, 1389, 1699
MaeI	CTAG	1 cut(s)	951
MaeII	ACGT	3 cut(s)	1194, 1579, 2109
MaeIII	GTNAC	4 cut(s)	549, 1348, 1374, 1625
MboII	GAAGA	9 cut(s)	35, 208, 223, 413, 520, 747, 1175, 1241, 2261
MflI	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2313, 2333
MhlI	GDGCHC	5 cut(s)	264, 1036, 1473, 1880, 2141
MlsI	TGGCCA	2 cut(s)	716, 2098
MluCI	AATT	7 cut(s)	460, 477, 796, 1614, 1749, 1867, 1985
MluNI	TGGCCA	2 cut(s)	716, 2098
MlyI	GAGTC	5 cut(s)	47, 512, 548, 1268, 1961
MmeI	TCCRAC	4 cut(s)	81, 1128, 2049, 2291
Mox20I	TGGCCA	2 cut(s)	716, 2098
Mph1103I	ATGCAT	4 cut(s)	310, 792, 1692, 1716
MroXI	GAANNNNTTC	1 cut(s)	1846
MscI	TGGCCA	2 cut(s)	716, 2098
MslI	CAYNNNNRTG	2 cut(s)	1466, 2079
Msp20I	TGGCCA	2 cut(s)	716, 2098
MspI	CCGG	5 cut(s)	167, 298, 318, 570, 1223
MspR9I	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2276, 2296
Mva1269I	GAATGC	2 cut(s)	790, 2219
MvaI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2276, 2296
MvnI	CGCG	1 cut(s)	2130
MwoI	GCNNNNNNNGC	9 cut(s)	167, 275, 346, 1040, 1088, 1711, 1901, 2136, 2242
NciI	CCSGG	3 cut(s)	167, 299, 318
NlaIV	GGNNCC	5 cut(s)	315, 1035, 1593, 1912, 2235
NmeAIII	GCCGAG	2 cut(s)	304, 1579
NmuCI	GTSAC	2 cut(s)	1374, 1625
NsiI	ATGCAT	4 cut(s)	310, 792, 1692, 1716
NspI	RCATGY	3 cut(s)	1521, 1714, 2257
NspV	TTCGAA	1 cut(s)	1442
PaeI	GCATGC	1 cut(s)	1521
PaeR7I	CTCGAG	1 cut(s)	1586
PagI	TCATGA	2 cut(s)	882, 1159
PciI	ACATGT	1 cut(s)	2253
PcsI	WCGNNNNNNNCGW	1 cut(s)	1576
PctI	GAATGC	2 cut(s)	790, 2219
PdmI	GAANNNNTTC	1 cut(s)	1846
PfeI	GAWTC	7 cut(s)	35, 113, 128, 677, 1121, 1601, 1847
PfoI	TCCNGGA	1 cut(s)	1036
PkrI	GCNGC	5 cut(s)	69, 172, 367, 397, 2281
PleI	GAGTC	5 cut(s)	47, 511, 547, 1268, 1961
PpsI	GAGTC	5 cut(s)	47, 511, 547, 1268, 1961
Ppu21I	YACGTR	1 cut(s)	1195
PpuMI	RGGWCCY	1 cut(s)	1062
PscI	ACATGT	1 cut(s)	2253
PshAI	GACNNNNGTC	1 cut(s)	416
PshBI	ATTAAT	1 cut(s)	1464
Psp124BI	GAGCTC	1 cut(s)	2141
Psp5II	RGGWCCY	1 cut(s)	1062
Psp6I	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2274, 2294
PspEI	GGTNACC	1 cut(s)	1625
PspGI	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2274, 2294
PspN4I	GGNNCC	5 cut(s)	315, 1035, 1593, 1912, 2235
PspPI	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
PspPPI	RGGWCCY	1 cut(s)	1062
PstI	CTGCAG	2 cut(s)	370, 400
PsuI	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2313, 2333
RsaI	GTAC	5 cut(s)	1370, 1671, 1774, 1808, 1912
RsaNI	GTAC	5 cut(s)	1369, 1670, 1773, 1807, 1911
RseI	CAYNNNNRTG	2 cut(s)	1466, 2079
SacI	GAGCTC	1 cut(s)	2141
SatI	GCNGC	5 cut(s)	68, 171, 366, 396, 2280
Sau96I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
ScaI	AGTACT	1 cut(s)	1808
SchI	GAGTC	5 cut(s)	47, 512, 548, 1268, 1961
ScrFI	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2276, 2296
SduI	GDGCHC	5 cut(s)	264, 1036, 1473, 1880, 2141
SfaNI	GCATC	5 cut(s)	94, 170, 564, 1389, 1699
SfcI	CTRYAG	4 cut(s)	88, 366, 396, 1887
Sfr274I	CTCGAG	1 cut(s)	1586
SfuI	TTCGAA	1 cut(s)	1442
SinI	GGWCC	2 cut(s)	313, 1062
SlaI	CTCGAG	1 cut(s)	1586
SmiMI	CAYNNNNRTG	2 cut(s)	1466, 2079
SmlI	CTYRAG	1 cut(s)	1586
SmoI	CTYRAG	1 cut(s)	1586
SnaBI	TACGTA	1 cut(s)	1195
SphI	GCATGC	1 cut(s)	1521
Sse9I	AATT	7 cut(s)	460, 477, 796, 1614, 1749, 1867, 1985
SsiI	CCGC	5 cut(s)	171, 1267, 1402, 1501, 1914
SspI	AATATT	1 cut(s)	1247
SspMI	CTAG	1 cut(s)	951
SstI	GAGCTC	1 cut(s)	2141
StyD4I	CCNGG	9 cut(s)	165, 297, 316, 1036, 1134, 1837, 2161, 2274, 2294
StyI	CCWWGG	2 cut(s)	1058, 1297
TaaI	ACNGT	4 cut(s)	92, 1420, 1743, 2194
TaiI	ACGT	3 cut(s)	1197, 1582, 2112
TaqI	TCGA	4 cut(s)	1219, 1442, 1587, 2055
TaqII	GACCGA	1 cut(s)	428
TasI	AATT	7 cut(s)	460, 477, 796, 1614, 1749, 1867, 1985
TatI	WGTACW	1 cut(s)	1806
TauI	GCSGC	1 cut(s)	173
TfiI	GAWTC	7 cut(s)	35, 113, 128, 677, 1121, 1601, 1847
TscAI	CASTG	5 cut(s)	905, 1435, 1885, 1903, 2268
TseFI	GTSAC	2 cut(s)	1374, 1625
TseI	GCWGC	4 cut(s)	67, 365, 395, 2279
Tsp45I	GTSAC	2 cut(s)	1374, 1625
TspGWI	ACGGA	1 cut(s)	1350
TspRI	CASTG	5 cut(s)	905, 1435, 1885, 1903, 2268
VneI	GTGCAC	1 cut(s)	260
VpaK11BI	GGWCC	2 cut(s)	313, 1062
VspI	ATTAAT	1 cut(s)	1464
XagI	CCTNNNNNAGG	1 cut(s)	1926
XapI	RAATTY	3 cut(s)	460, 1749, 1985
XceI	RCATGY	3 cut(s)	1521, 1714, 2257
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2253, 2307
XhoI	CTCGAG	1 cut(s)	1586
XmnI	GAANNNNTTC	1 cut(s)	1846
XspI	CTAG	1 cut(s)	951
ZrmI	AGTACT	1 cut(s)	1808
Zsp2I	ATGCAT	4 cut(s)	310, 792, 1692, 1716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.