Rh5AG407400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007476
Physical Location & Seq
Forward (+)
70374273 .. 70379599
5327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG407400.1

Sequence Viewer

Length: 462 bp
ATGGCCAGCAGCAACAACCAGAGCTCCTCAAGCTCCCATGGTAATACCCTTTACAAAATTCCTTGTTGGGTCACACCGGATAAGAAGGAGTTTCACAATAGAACAATGGAGTTCCTTACGTGGTACCGGAAGTCTAACCGCACCTTGCCTTTGATAAATCAAAGGAAATCAGTAATGGAGATTCAGCTCTTAAAGAGTGAATCATTCTCTATCAATCCTTCCCCCTCCAATAAATGCATGAAGGTCAAAGATATGTCTCGCGAAGAAGTATTGGAGAAAATCAGGGAGGAGAAGAAACGTATTGAAGATGATTGTTCCTTTGAGTTGGTTGATCAATTGGCCTCCAACAAAATGTTCGATGACGGTGCCCAACGTTTAGTTCTAAAAGACGGCAAAAAACTTTACAAGCCGCAAAAGGCTACAAAAGAAGCCTATATATGGTCGTGTTCTAAATGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.84

Weight (kDa)

9.2

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 2 cut(s) 123, 365
AccII CGCG 1 cut(s) 261
AciI CCGC 2 cut(s) 139, 410
AclI AACGTT 1 cut(s) 373
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 57
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 438
AgsI TTSAA 1 cut(s) 305
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 363, 370, 395
AluBI AGCT 3 cut(s) 24, 33, 187
AluI AGCT 3 cut(s) 24, 33, 187
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 1 cut(s) 261
AoxI GGCC 2 cut(s) 3, 339
ApeKI GCWGC 1 cut(s) 9
ApoI RAATTY 1 cut(s) 57
Asp718I GGTACC 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 370
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 123, 365
BanII GRGCYC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 21
BceAI ACGGC 1 cut(s) 406
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 261
BisI GCNGC 2 cut(s) 10, 410
BlsI GCNGC 2 cut(s) 11, 411
BmiI GGNNCC 2 cut(s) 125, 367
BpuEI CTTGAG 1 cut(s) 13
BsaAI YACGTR 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 37
BsaWI WCCGGW 2 cut(s) 76, 126
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 438
BseRI GAGGAG 2 cut(s) 16, 302
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 1 cut(s) 21
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 2 cut(s) 5, 341
BshNI GGYRCC 2 cut(s) 123, 365
BsiHKAI GWGCWC 1 cut(s) 26
BsiSI CCGG 2 cut(s) 77, 127
BslI CCNNNNNNNGG 1 cut(s) 438
BsmAI GTCTC 1 cut(s) 261
BsnI GGCC 2 cut(s) 5, 341
Bsp1286I GDGCHC 2 cut(s) 26, 370
Bsp143I GATC 1 cut(s) 331
Bsp19I CCATGG 1 cut(s) 37
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 2 cut(s) 139, 410
BspANI GGCC 2 cut(s) 5, 341
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 125, 367
BspT107I GGYRCC 2 cut(s) 123, 365
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 1 cut(s) 331
BssT1I CCWWGG 1 cut(s) 37
Bst4CI ACNGT 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 7
BstDSI CCRYGG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 261
BstKTI GATC 1 cut(s) 334
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 370
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 21
BsuRI GGCC 2 cut(s) 5, 341
BtgI CCRYGG 1 cut(s) 37
BtuMI TCGCGA 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 7
Csp6I GTAC 1 cut(s) 124
CviAII CATG 2 cut(s) 38, 238
CviJI RGCY 8 cut(s) 5, 24, 33, 187, 341, 409, 419, 431
CviKI_1 RGCY 8 cut(s) 5, 24, 33, 187, 341, 409, 419, 431
CviQI GTAC 1 cut(s) 124
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 37
EcoT22I ATGCAT 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 2 cut(s) 41, 241
FaiI YATR 6 cut(s) 39, 239, 254, 435, 437, 439
FatI CATG 2 cut(s) 37, 237
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 10, 410
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 2 cut(s) 10, 410
GluI GCNGC 2 cut(s) 10, 410
HaeIII GGCC 2 cut(s) 5, 341
HapII CCGG 2 cut(s) 77, 127
Hin1II CATG 2 cut(s) 41, 241
HinfI GANTC 2 cut(s) 181, 200
HpaII CCGG 2 cut(s) 77, 127
Hpy188III TCNNGA 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 79, 228, 235
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 3 cut(s) 119, 298, 373
HpyCH4V TGCA 1 cut(s) 237
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpySE526I ACGT 3 cut(s) 119, 298, 373
Hsp92II CATG 2 cut(s) 41, 241
KpnI GGTACC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 1 cut(s) 331
LmnI GCTCC 2 cut(s) 29, 38
LpnPI CCDG 5 cut(s) 19, 32, 90, 140, 268
Lsp1109I GCAGC 1 cut(s) 21
MaeII ACGT 3 cut(s) 119, 298, 373
MaeIII GTNAC 1 cut(s) 70
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 3 cut(s) 275, 304, 317
MfeI CAATTG 1 cut(s) 335
MhlI GDGCHC 2 cut(s) 26, 370
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 57, 335
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 4 cut(s) 37, 235, 280, 352
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 239
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 191, 460
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 77, 127
MunI CAATTG 1 cut(s) 335
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 1 cut(s) 30
NcoI CCATGG 1 cut(s) 37
NdeII GATC 1 cut(s) 331
NlaIII CATG 2 cut(s) 41, 241
NlaIV GGNNCC 2 cut(s) 125, 367
NmuCI GTSAC 1 cut(s) 70
NruI TCGCGA 1 cut(s) 261
NsiI ATGCAT 1 cut(s) 239
PfeI GAWTC 2 cut(s) 181, 200
PkrI GCNGC 2 cut(s) 11, 411
Ppu21I YACGTR 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 26
Psp1406I AACGTT 1 cut(s) 373
PspN4I GGNNCC 2 cut(s) 125, 367
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 191, 460
SatI GCNGC 2 cut(s) 10, 410
Sau3AI GATC 1 cut(s) 331
SduI GDGCHC 2 cut(s) 26, 370
SetI ASST 8 cut(s) 26, 35, 122, 146, 189, 246, 301, 376
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 2 cut(s) 57, 335
SsiI CCGC 2 cut(s) 139, 410
SstI GAGCTC 1 cut(s) 26
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 3 cut(s) 122, 301, 376
TaqI TCGA 1 cut(s) 357
TasI AATT 2 cut(s) 57, 335
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 2 cut(s) 181, 200
Tru1I TTAA 2 cut(s) 191, 460
Tru9I TTAA 2 cut(s) 191, 460
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 1 cut(s) 9
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 254
XapI RAATTY 1 cut(s) 57
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.