Rorug05G0382700

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016979
Physical Location & Seq
Forward (+)
51914243 .. 51916612
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0382700.1

Sequence Viewer

Length: 657 bp
ATGACCCCTACCTTGTTAGACATGGCGACAATATTTGGGTTTCGGCCAAATGGTGTAGCGGTTTGTGCTACCACCAAATTTCCGACTTCCTTCCACACTAGCTTGAAGCCCAAAGCGGTCAAAAACGACAAGAAGGGTAAAGATGAGGCAGATGCTAAGGCAAAACAATTACTCAACTATAGCACCTTCTATGCCACACATGTGGTTGATGAAGAGGTGACAGAAAATAGGAGACAACCCCCCAAGAAACATGAACACGCAACTTTCCTCCTCTATTGGCTTTGTAAGTTTGTATTTTGTGCCAAATCCAATAAGTGCACTTTCGAGTTTGCTGGTATAGCGGAAGCACTAAGTGCGGTTGGTGTCTCTGACTCAAATCCCATTGCTTCTGGATCTGGGGGTTCACAGTTTAGAGTCTCGAAAATGGTAACAGATGCAGATTTGAAGTTTCTGATTGAAAATTTGGACGAGAAGATCGGTCAGAATAAGAAATGGGACAATGTCATAGAGAAAGGAAATGATCTTCTATACTACAGTGCTAAGTGCTGCAAGCCTAAGGTTGGTCCTGTGAAATACTTGAGTGTGACAATATTTGAGGACTGCTCTCCTGAGATGCTGAGAGACTTCTACATGGATAATGATTACAGAAAGCAATGA

Protein Analysis

218

Amino Acids

24.53

Weight (kDa)

8.56

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 1 - 119 3e-09 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 59, 116, 341, 356
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 44
AcsI RAATTY 2 cut(s) 77, 460
AdeI CACNNNGTG 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 560
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 3 cut(s) 106, 445, 458
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 1 cut(s) 102
AluI AGCT 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 4 cut(s) 226, 370, 421, 615
Alw44I GTGCAC 1 cut(s) 316
AlwI GGATC 1 cut(s) 400
AoxI GGCC 1 cut(s) 44
ApaLI GTGCAC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 2 cut(s) 77, 460
AspS9I GGNCC 1 cut(s) 563
AsuHPI GGTGA 1 cut(s) 229
AvaII GGWCC 1 cut(s) 563
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 533
BcoDI GTCTC 4 cut(s) 226, 370, 421, 615
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 2 cut(s) 178, 532
BisI GCNGC 1 cut(s) 547
BlsI GCNGC 1 cut(s) 548
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 563
BmsI GCATC 3 cut(s) 142, 424, 603
BplI GAGNNNNNCTC 2 cut(s) 587, 619
Bpu10I CCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 598
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 560
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 600, 608
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 1 cut(s) 533
BshFI GGCC 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 320
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 4 cut(s) 226, 370, 421, 615
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 1 cut(s) 46
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 3 cut(s) 392, 474, 520
BspACI CCGC 4 cut(s) 59, 116, 341, 356
BspANI GGCC 1 cut(s) 46
BspCNI CTCAG 2 cut(s) 601, 609
BspPI GGATC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 381
BssMI GATC 3 cut(s) 392, 474, 520
Bst4CI ACNGT 2 cut(s) 408, 536
Bst6I CTCTTC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 551
BstDEI CTNAG 6 cut(s) 156, 350, 540, 555, 609, 617
BstKTI GATC 3 cut(s) 395, 477, 523
BstMAI GTCTC 4 cut(s) 226, 370, 421, 615
BstMBI GATC 3 cut(s) 392, 474, 520
BstMWI GCNNNNNNNGC 3 cut(s) 65, 338, 353
BstNSI RCATGY 1 cut(s) 203
BstSFI CTRYAG 2 cut(s) 178, 532
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 533
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 392
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 541
Cac8I GCNNGC 1 cut(s) 551
Cfr13I GGNCC 1 cut(s) 563
CviAII CATG 4 cut(s) 22, 200, 251, 631
CviJI RGCY 5 cut(s) 46, 102, 109, 280, 553
CviKI_1 RGCY 5 cut(s) 46, 102, 109, 280, 553
DdeI CTNAG 6 cut(s) 156, 350, 540, 555, 609, 617
DpnI GATC 3 cut(s) 394, 476, 522
DpnII GATC 3 cut(s) 392, 474, 520
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 563
Eco81I CCTNAGG 1 cut(s) 555
FaeI CATG 4 cut(s) 25, 203, 254, 634
FaiI YATR 9 cut(s) 23, 180, 192, 201, 252, 338, 506, 529, 632
FaqI GGGAC 1 cut(s) 509
FatI CATG 4 cut(s) 21, 199, 250, 630
Fnu4HI GCNGC 1 cut(s) 547
Fsp4HI GCNGC 1 cut(s) 547
FspBI CTAG 1 cut(s) 99
GluI GCNGC 1 cut(s) 547
HaeIII GGCC 1 cut(s) 46
Hin1II CATG 4 cut(s) 25, 203, 254, 634
HinfI GANTC 2 cut(s) 371, 414
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 2 cut(s) 318, 404
Hpy188I TCNGA 4 cut(s) 84, 370, 453, 483
Hpy188III TCNNGA 3 cut(s) 390, 418, 608
Hpy8I GTNNAC 2 cut(s) 318, 404
HpyAV CCTTC 3 cut(s) 100, 127, 196
HpyCH4III ACNGT 2 cut(s) 408, 536
HpyCH4V TGCA 3 cut(s) 318, 437, 549
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 338, 353
HpyF3I CTNAG 6 cut(s) 156, 350, 540, 555, 609, 617
Hsp92II CATG 4 cut(s) 25, 203, 254, 634
Kzo9I GATC 3 cut(s) 392, 474, 520
LpnPI CCDG 5 cut(s) 318, 375, 381, 579, 621
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 3 cut(s) 142, 424, 603
MaeI CTAG 1 cut(s) 99
MaeIII GTNAC 3 cut(s) 217, 427, 583
MalI GATC 3 cut(s) 394, 476, 522
MboI GATC 3 cut(s) 392, 474, 520
MboII GAAGA 3 cut(s) 224, 484, 515
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 3 cut(s) 77, 167, 460
MlyI GAGTC 2 cut(s) 365, 423
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 5 cut(s) 139, 208, 278, 281, 589
MslI CAYNNNNRTG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 65, 338, 353
NdeII GATC 3 cut(s) 392, 474, 520
NlaIII CATG 4 cut(s) 25, 203, 254, 634
NmuCI GTSAC 2 cut(s) 217, 583
NspI RCATGY 1 cut(s) 203
OliI CACNNNNGTG 1 cut(s) 200
PciI ACATGT 1 cut(s) 199
PflFI GACNNNGTC 1 cut(s) 500
PkrI GCNGC 1 cut(s) 548
PleI GAGTC 2 cut(s) 365, 422
PpsI GAGTC 2 cut(s) 365, 422
PscI ACATGT 1 cut(s) 199
PspPI GGNCC 1 cut(s) 563
PsuI RGATCY 1 cut(s) 392
PsyI GACNNNGTC 1 cut(s) 500
RseI CAYNNNNRTG 1 cut(s) 200
SatI GCNGC 1 cut(s) 547
Sau3AI GATC 3 cut(s) 392, 474, 520
Sau96I GGNCC 1 cut(s) 563
SchI GAGTC 2 cut(s) 365, 423
SduI GDGCHC 1 cut(s) 320
SetI ASST 5 cut(s) 14, 104, 188, 219, 561
SfaNI GCATC 3 cut(s) 142, 424, 603
SfcI CTRYAG 2 cut(s) 178, 532
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
Sse9I AATT 3 cut(s) 77, 167, 460
SsiI CCGC 4 cut(s) 59, 116, 341, 356
SspI AATATT 2 cut(s) 33, 591
SspMI CTAG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 408, 536
TaqI TCGA 2 cut(s) 324, 419
TaqII GACCGA 1 cut(s) 467
TasI AATT 3 cut(s) 77, 167, 460
TscAI CASTG 1 cut(s) 541
TseFI GTSAC 2 cut(s) 217, 583
TseI GCWGC 1 cut(s) 546
Tsp45I GTSAC 2 cut(s) 217, 583
TspDTI ATGAA 2 cut(s) 225, 267
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 500
VneI GTGCAC 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 563
XapI RAATTY 2 cut(s) 77, 460
XceI RCATGY 1 cut(s) 203
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.