Rorug05G0409300
ERF Family

Belongs to the small GTPase superfamily. Rho family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018257
Physical Location & Seq
Reverse (-)
56517940 .. 56518598
659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0409300.1

Sequence Viewer

Length: 369 bp
ATGGCCAAGTCTAGAAGTACTATCAGTGGCAAGAAGAAGAACAGCATAGTGAAGCTCAAGGTTGTAGTAGAAAAGCTGCAGCGGAGTCTATTGTTGACCCGGAAATCCAATTCAAGTTTTGAAGACGACTCTACCTCAGTGCCCGAAGACGTTAAGGAAGGTCACTTTGCGGTGATTGCCGTGGACGGGGACAAACCGAAGAGATTCGTGGTGCCGTTAAGTTATCTGGCTCACCCAACTTTCTTGAAGCTGCTGGAGCAGGCGGCCGAGGAGTACGGTTTTGATCACTCGGGTGCCCTAGCGATTCCTTGCCTGCCAAGCGAGCTTGAGAAGATATTAAGTGATGAGCAGTGGCAGAAGAAAGACTGA

Protein Analysis

122

Amino Acids

13.61

Weight (kDa)

7.83

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 24 - 113 5.3e-21 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018257)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002701.1_g000001
rosa_roxburghii Rroxscaffold_1G00012900
rosa_rugosa Rorug05G0409300 Rorug05G0409400 Rorug05G0409500 Rorug05G0409600
rosa_samantha Rh2AG431000 Rh2CG416700 Rh6CG162200 Rh6DG153800
rosa_wichuraiana Rw3G029270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 211, 293
AciI CCGC 3 cut(s) 82, 170, 263
AcoI YGGCCR 2 cut(s) 3, 264
AfaI GTAC 2 cut(s) 19, 275
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 3 cut(s) 114, 122, 247
AleI CACNNNNGTG 1 cut(s) 291
AluBI AGCT 4 cut(s) 55, 76, 250, 325
AluI AGCT 4 cut(s) 55, 76, 250, 325
Ama87I CYCGRG 1 cut(s) 289
AoxI GGCC 2 cut(s) 3, 264
ApeKI GCWGC 3 cut(s) 76, 79, 250
Asp700I GAANNNNTTC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 2 cut(s) 184, 224
AvaI CYCGRG 1 cut(s) 289
BaeGI GKGCMC 2 cut(s) 144, 298
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 211, 293
BbsI GAAGAC 2 cut(s) 129, 153
BbvI GCAGC 3 cut(s) 63, 91, 237
BceAI ACGGC 2 cut(s) 164, 199
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 100
BfaI CTAG 2 cut(s) 12, 299
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 4 cut(s) 77, 80, 251, 264
BlsI GCNGC 4 cut(s) 78, 81, 252, 265
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 289
BmiI GGNNCC 2 cut(s) 213, 295
BmrFI CCNGG 1 cut(s) 100
BpiI GAAGAC 2 cut(s) 129, 153
BpmI CTGGAG 1 cut(s) 275
BpuEI CTTGAG 2 cut(s) 41, 347
BpuMI CCSGG 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 180, 267
Bsc4I CCNNNNNNNGG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 180, 267
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 284
BseSI GKGCMC 2 cut(s) 144, 298
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 3 cut(s) 63, 91, 237
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 2 cut(s) 5, 266
BshNI GGYRCC 2 cut(s) 211, 293
BsiEI CGRYCG 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 1 cut(s) 100
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 2 cut(s) 5, 266
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 144, 298
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 3 cut(s) 82, 170, 263
BspANI GGCC 2 cut(s) 5, 266
BspCNI CTCAG 1 cut(s) 149
BspLI GGNNCC 2 cut(s) 213, 295
BspMAI CTGCAG 1 cut(s) 81
BspT107I GGYRCC 2 cut(s) 211, 293
BssECI CCNNGG 2 cut(s) 180, 267
BssMI GATC 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 1 cut(s) 194
BstC8I GCNNGC 3 cut(s) 261, 314, 323
BstDEI CTNAG 1 cut(s) 136
BstDSI CCRYGG 1 cut(s) 180
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 4 cut(s) 176, 256, 318, 322
BstSCI CCNGG 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 77
BstSLI GKGCMC 2 cut(s) 144, 298
BstV1I GCAGC 3 cut(s) 63, 91, 237
BstV2I GAAGAC 2 cut(s) 129, 153
BstZI CGGCCG 1 cut(s) 264
BsuRI GGCC 2 cut(s) 5, 266
BtgI CCRYGG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 356
BtsIMutI CAGTG 3 cut(s) 31, 144, 356
Cac8I GCNNGC 3 cut(s) 261, 314, 323
Csp6I GTAC 2 cut(s) 18, 274
CviJI RGCY 7 cut(s) 5, 55, 76, 230, 250, 266, 325
CviKI_1 RGCY 7 cut(s) 5, 55, 76, 230, 250, 266, 325
CviQI GTAC 2 cut(s) 18, 274
DdeI CTNAG 1 cut(s) 136
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
EaeI YGGCCR 2 cut(s) 3, 264
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EclXI CGGCCG 1 cut(s) 264
Eco52I CGGCCG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 289
FaiI YATR 1 cut(s) 47
FaqI GGGAC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 77, 80, 251, 264
Fsp4HI GCNGC 4 cut(s) 77, 80, 251, 264
FspBI CTAG 2 cut(s) 12, 299
GluI GCNGC 4 cut(s) 77, 80, 251, 264
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 2 cut(s) 5, 266
HapII CCGG 1 cut(s) 100
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HinfI GANTC 4 cut(s) 85, 128, 204, 304
HpaII CCGG 1 cut(s) 100
HphI GGTGA 2 cut(s) 184, 224
Hpy166II GTNNAC 2 cut(s) 96, 184
Hpy188III TCNNGA 2 cut(s) 12, 244
Hpy8I GTNNAC 2 cut(s) 96, 184
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 79
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 256, 318, 322
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 256
LpnPI CCDG 5 cut(s) 113, 212, 239, 245, 326
Lsp1109I GCAGC 3 cut(s) 63, 91, 237
MaeI CTAG 2 cut(s) 12, 299
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 161
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 6 cut(s) 46, 49, 134, 158, 211, 343
MhlI GDGCHC 2 cut(s) 144, 298
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 109
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 94, 122
MnlI CCTC 2 cut(s) 145, 262
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 153, 218, 338
MslI CAYNNNNRTG 1 cut(s) 291
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 82
MspI CCGG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 100
MwoI GCNNNNNNNGC 4 cut(s) 176, 256, 318, 322
NciI CCSGG 1 cut(s) 100
NdeII GATC 1 cut(s) 283
NlaIV GGNNCC 2 cut(s) 213, 295
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 161
OliI CACNNNNGTG 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 204, 304
PkrI GCNGC 4 cut(s) 78, 81, 252, 265
PleI GAGTC 2 cut(s) 93, 122
PpsI GAGTC 2 cut(s) 93, 122
PspN4I GGNNCC 2 cut(s) 213, 295
PstI CTGCAG 1 cut(s) 81
RsaI GTAC 2 cut(s) 19, 275
RsaNI GTAC 2 cut(s) 18, 274
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 3 cut(s) 153, 218, 338
SatI GCNGC 4 cut(s) 77, 80, 251, 264
Sau3AI GATC 1 cut(s) 283
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 2 cut(s) 94, 122
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 2 cut(s) 144, 298
SetI ASST 8 cut(s) 57, 63, 78, 137, 153, 163, 252, 327
SfcI CTRYAG 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 2 cut(s) 56, 326
SmoI CTYRAG 2 cut(s) 56, 326
Sse9I AATT 1 cut(s) 109
SsiI CCGC 3 cut(s) 82, 170, 263
SspMI CTAG 2 cut(s) 12, 299
StyD4I CCNGG 1 cut(s) 98
TaaI ACNGT 1 cut(s) 278
TaiI ACGT 1 cut(s) 153
TasI AATT 1 cut(s) 109
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 2 cut(s) 204, 304
Tru1I TTAA 3 cut(s) 153, 218, 338
Tru9I TTAA 3 cut(s) 153, 218, 338
TscAI CASTG 3 cut(s) 31, 144, 356
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 3 cut(s) 76, 79, 250
Tsp45I GTSAC 1 cut(s) 161
TspRI CASTG 3 cut(s) 31, 144, 356
XbaI TCTAGA 1 cut(s) 11
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 2 cut(s) 12, 299
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.