Rorug05G0518800

auxin-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014651
Physical Location & Seq
Forward (+)
70301185 .. 70301929
745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0518800.1

Sequence Viewer

Length: 516 bp
ATGAACGCTACCTCTCCTAACGATTTCATGGAAGCCGTAGAGAATCACAGCGGAAGCGATTCCGACACCAACATCGACGACGACGATGCCGCCGCCGAGTACTACCAGCCAGTCTCCGCCGTCGATTCCGAAGACGACGAGGAGAATCACCATCTCCAAAACGACGCCGTTGTTTCCCACTCGCAGCTGCTCCCCAACGGCGTGATTGTGAACCAGGCGCAGAGGGGAGTGTCGTCGCTGAGATTAAACGACGACGTCGAGGGTGATAATGGCAGTAGCAGCGAAGAAGAAGAAGAAGATGTGATTGGAGGGGGTTCTGATTCGGCGGTCACGAGGGCGATCAGGGAAGACGAGAGTCGCCGGCACGCGCCGTTGACGCCGGAGAATGCGACGAGGGTTATGGAGGCGATGAGAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCTGATCGGATCCCTGAAGAAAACTGGATCGATCGGCTTCGCCGGCTCCGACAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

171

Amino Acids

18.77

Weight (kDa)

4.2

Isoelectric Point (pI)

63.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 33 - 164 4.7e-07 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccII CGCG 1 cut(s) 368
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 5 cut(s) 51, 90, 93, 117, 326
AclWI GGATC 3 cut(s) 451, 464, 482
AcuI CTGAAG 1 cut(s) 483
AcyI GRCGYC 3 cut(s) 165, 255, 377
AfaI GTAC 1 cut(s) 101
AjnI CCWGG 1 cut(s) 213
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 1 cut(s) 187
AluI AGCT 1 cut(s) 187
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 3 cut(s) 451, 464, 482
Aor13HI TCCGGA 1 cut(s) 439
ApeKI GCWGC 3 cut(s) 184, 187, 279
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 220, 370
AsuHPI GGTGA 3 cut(s) 140, 275, 495
BamHI GGATCC 1 cut(s) 456
BauI CACGAG 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 138, 354
BbvI GCAGC 3 cut(s) 174, 196, 291
BccI CCATC 1 cut(s) 159
BceAI ACGGC 5 cut(s) 20, 104, 152, 214, 355
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 118
BglI GCCNNNNNGGC 1 cut(s) 434
BisI GCNGC 5 cut(s) 90, 93, 185, 188, 280
BlsI GCNGC 5 cut(s) 91, 94, 186, 189, 281
BmcAI AGTACT 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 215
BmiI GGNNCC 3 cut(s) 438, 458, 494
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 76
BoxI GACNNNNGTC 1 cut(s) 354
BpiI GAAGAC 2 cut(s) 138, 354
Bsa29I ATCGAT 1 cut(s) 477
BsaHI GRCGYC 3 cut(s) 165, 255, 377
BsaWI WCCGGW 1 cut(s) 439
Bse118I RCCGGY 2 cut(s) 360, 489
Bse1I ACTGG 2 cut(s) 110, 476
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 1 cut(s) 215
BseCI ATCGAT 1 cut(s) 477
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 2 cut(s) 110, 476
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 3 cut(s) 174, 196, 291
Bsh1236I CGCG 1 cut(s) 368
Bsh1285I CGRYCG 1 cut(s) 481
BshVI ATCGAT 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 481
BsiSI CCGG 5 cut(s) 361, 380, 429, 440, 490
BsmAI GTCTC 1 cut(s) 118
BsmI GAATGC 1 cut(s) 391
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 5 cut(s) 339, 451, 456, 474, 478
BspACI CCGC 5 cut(s) 51, 90, 93, 117, 326
BspCNI CTCAG 1 cut(s) 231
BspDI ATCGAT 1 cut(s) 477
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 3 cut(s) 438, 458, 494
BspPI GGATC 3 cut(s) 451, 464, 482
BsrFI RCCGGY 2 cut(s) 360, 489
BsrI ACTGG 2 cut(s) 110, 476
BssAI RCCGGY 2 cut(s) 360, 489
BssMI GATC 5 cut(s) 339, 451, 456, 474, 478
BssNI GRCGYC 3 cut(s) 165, 255, 377
BssSI CACGAG 1 cut(s) 331
Bst2BI CACGAG 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 501
BstACI GRCGYC 3 cut(s) 165, 255, 377
BstC8I GCNNGC 3 cut(s) 362, 366, 491
BstDEI CTNAG 1 cut(s) 239
BstFNI CGCG 1 cut(s) 368
BstHHI GCGC 2 cut(s) 220, 370
BstKTI GATC 5 cut(s) 342, 454, 459, 477, 481
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 5 cut(s) 339, 451, 456, 474, 478
BstMCI CGRYCG 1 cut(s) 481
BstMWI GCNNNNNNNGC 4 cut(s) 279, 376, 434, 490
BstNI CCWGG 1 cut(s) 215
BstPAI GACNNNNGTC 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 213
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 3 cut(s) 174, 196, 291
BstV2I GAAGAC 2 cut(s) 138, 354
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
Bsu15I ATCGAT 1 cut(s) 477
BsuTUI ATCGAT 1 cut(s) 477
BtgZI GCGATG 1 cut(s) 422
Cac8I GCNNGC 3 cut(s) 362, 366, 491
CfoI GCGC 2 cut(s) 220, 370
Cfr10I RCCGGY 2 cut(s) 360, 489
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 2 cut(s) 173, 385
Csp6I GTAC 1 cut(s) 100
CviAII CATG 1 cut(s) 28
CviJI RGCY 7 cut(s) 35, 109, 187, 437, 449, 484, 493
CviKI_1 RGCY 7 cut(s) 35, 109, 187, 437, 449, 484, 493
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 239
DpnI GATC 5 cut(s) 341, 453, 458, 476, 480
DpnII GATC 5 cut(s) 339, 451, 456, 474, 478
EciI GGCGGA 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 213
FaeI CATG 1 cut(s) 31
FaiI YATR 2 cut(s) 29, 401
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 5 cut(s) 90, 93, 185, 188, 280
Fsp4HI GCNGC 5 cut(s) 90, 93, 185, 188, 280
GlaI GCGC 2 cut(s) 219, 369
GluI GCNGC 5 cut(s) 90, 93, 185, 188, 280
HapII CCGG 5 cut(s) 361, 380, 429, 440, 490
HgaI GACGC 2 cut(s) 173, 385
HhaI GCGC 2 cut(s) 220, 370
Hin1I GRCGYC 3 cut(s) 165, 255, 377
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 218, 368
HinP1I GCGC 2 cut(s) 218, 368
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 6 cut(s) 43, 59, 125, 145, 320, 355
HpaII CCGG 5 cut(s) 361, 380, 429, 440, 490
HphI GGTGA 3 cut(s) 140, 275, 495
Hpy166II GTNNAC 2 cut(s) 211, 375
Hpy188I TCNGA 5 cut(s) 64, 130, 319, 456, 497
Hpy188III TCNNGA 2 cut(s) 331, 440
Hpy8I GTNNAC 2 cut(s) 211, 375
HpyCH4III ACNGT 1 cut(s) 501
HpyCH4IV ACGT 1 cut(s) 255
HpyF10VI GCNNNNNNNGC 4 cut(s) 279, 376, 434, 490
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 3 cut(s) 165, 255, 377
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 218, 368
Kpn2I TCCGGA 1 cut(s) 439
KroI GCCGGC 2 cut(s) 360, 489
KroNI GCCGGC 2 cut(s) 362, 491
Kzo9I GATC 5 cut(s) 339, 451, 456, 474, 478
LmnI GCTCC 3 cut(s) 195, 442, 498
Lsp1109I GCAGC 3 cut(s) 174, 196, 291
LweI GCATC 1 cut(s) 76
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 328, 501
MalI GATC 5 cut(s) 341, 453, 458, 476, 480
MboI GATC 5 cut(s) 339, 451, 456, 474, 478
MboII GAAGA 8 cut(s) 143, 296, 299, 302, 305, 308, 359, 476
MflI RGATCY 1 cut(s) 456
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 9 cut(s) 22, 133, 216, 253, 302, 327, 387, 397, 407
MroI TCCGGA 1 cut(s) 439
MroNI GCCGGC 2 cut(s) 360, 489
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 2 cut(s) 245, 514
MspA1I CMGCKG 2 cut(s) 51, 187
MspI CCGG 5 cut(s) 361, 380, 429, 440, 490
MspR9I CCNGG 1 cut(s) 215
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 4 cut(s) 279, 376, 434, 490
NaeI GCCGGC 2 cut(s) 362, 491
NdeII GATC 5 cut(s) 339, 451, 456, 474, 478
NgoMIV GCCGGC 2 cut(s) 360, 489
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 3 cut(s) 438, 458, 494
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 2 cut(s) 328, 501
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 255, 329, 493
PctI GAATGC 1 cut(s) 391
PdiI GCCGGC 2 cut(s) 362, 491
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 5 cut(s) 43, 59, 125, 145, 320
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 5 cut(s) 91, 94, 186, 189, 281
Ple19I CGATCG 1 cut(s) 481
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 3 cut(s) 438, 458, 494
PsuI RGATCY 1 cut(s) 456
PsyI GACNNNGTC 1 cut(s) 254
PvuI CGATCG 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 2 cut(s) 245, 514
SatI GCNGC 5 cut(s) 90, 93, 185, 188, 280
Sau3AI GATC 5 cut(s) 339, 451, 456, 474, 478
ScaI AGTACT 1 cut(s) 101
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 1 cut(s) 215
SetI ASST 3 cut(s) 14, 189, 258
SfaNI GCATC 1 cut(s) 76
SsiI CCGC 5 cut(s) 51, 90, 93, 117, 326
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 501
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 75, 123, 258, 477
TatI WGTACW 1 cut(s) 99
TauI GCSGC 2 cut(s) 92, 95
TfiI GAWTC 5 cut(s) 43, 59, 125, 145, 320
Tru1I TTAA 2 cut(s) 245, 514
Tru9I TTAA 2 cut(s) 245, 514
TseFI GTSAC 2 cut(s) 328, 501
TseI GCWGC 3 cut(s) 184, 187, 279
Tsp45I GTSAC 2 cut(s) 328, 501
TspDTI ATGAA 2 cut(s) 16, 17
Tth111I GACNNNGTC 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 58
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.