Rorug05G0529000

Protein of unknown function (DUF1005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016745
Physical Location & Seq
Reverse (-)
71812357 .. 71813855
1499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0529000.1

Sequence Viewer

Length: 909 bp
ATGGTGACAGAATTTTGGTACAAGCTCGACAGTACATTTAAACTTCCATATGTAAATACATACTTCCGCATCAATCTGAAGGGTGCATATGATGATGTGAAGAGTTGTGTTTTGACTGGCTTATACCTTGACCTTCTCACAGATCAGCTGAATGAGATCCTCTATGAGGTCAGTGTCTCGTCTCTTATAGCTAGTCATTTTGCAGTAGAAGCAATTAGTCTTGCAAATTTATTTCAAACAAATTTTACTGTACCACCACTTCCTACCAAATTGGTGTATAAAGACCATTGTATCTGTCTTCCTCCAAATACTAACCCTGTTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAACTCTGTGACTGAGCTGTATTTTCAAATTGAGCAGGCAGTGAAGAGTGAGTCCGTCAGACTAAAAGTATTGATAGACCTTTTTCAGGAAATTGTACAGGAACCACTTTTTAATCAACTAAGGACGAAGGAGCAGCTTGGGTATGTTGTTCTCTGTGGCCAGAATTGTACGTGCAATGTTTTTGGCTTCTATTTCTGTGTTCAGTCATCCGAGTACAACCCAATCCACCTTCAAGGCAGACTGGACAACCTTATAGATGGTCTGGAAGAGTTGTTGGAAGGGCTGGACGATGATTCCTTTGAGAATTATAAAGGTGGACTAATGGCCAAGGTTTTGGAGAAAGATGCATCCCTCACGTCTGAAACCAATCGATTGTGGACTCAGATTCTTGATAAATGGTATACGTTTGCCTATTCGAAAAAGGTCGCAGAACAACTCAGAAGCATTCAGAAGGAGGATGTTATCAACTTTTACAAGATCAACATATTTGCAACAATCATCTCCAAAGCGTTGAAGACTTGCAACTCGTGTATGGGGTTGCAATCAGCGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1005

Protein Analysis

302

Amino Acids

34.52

Weight (kDa)

5.07

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_M PF16187 3 - 62 5.8e-13 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 101 - 223 1.1e-13 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 145 - 244 2.3e-23 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016745)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03010
malus_domestica MD05G1108400.v1.1
prunus_persica Prupe.8G152100_v2.0.a1
pyrus_communis pycom05g10440
rosa_chinensis RchiOBHm_Chr6g0247801
rosa_laevigata RLG00000015178
rosa_roxburghii Rroxscaffold_180G00433230 Rroxscaffold_7G00213640
rosa_rugosa Rorug05G0529000
rosa_samantha Rh6AG043800 Rh6BG038600 Rh6CG037300 Rh6DG034400
rosa_wichuraiana Rw6G003800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 663
AccI GTMKAC 1 cut(s) 755
AciI CCGC 1 cut(s) 67
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 2 cut(s) 511, 678
AcsI RAATTY 3 cut(s) 11, 226, 241
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 6 cut(s) 20, 34, 252, 450, 523, 569
AfiI CCNNNNNNNGG 2 cut(s) 166, 439
AgsI TTSAA 4 cut(s) 236, 380, 587, 868
AjiI CACGTC 1 cut(s) 711
AluBI AGCT 5 cut(s) 25, 148, 191, 370, 490
AluI AGCT 5 cut(s) 25, 148, 191, 370, 490
Alw26I GTCTC 2 cut(s) 181, 186
AlwI GGATC 1 cut(s) 151
AoxI GGCC 2 cut(s) 511, 678
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 3 cut(s) 11, 226, 241
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 770
BaeI ACNNNNGTAYC 2 cut(s) 274, 307
BalI TGGCCA 2 cut(s) 513, 680
BarI GAAGNNNNNNTAC 2 cut(s) 243, 275
BauI CACGAG 1 cut(s) 880
BbsI GAAGAC 2 cut(s) 290, 875
BbvI GCAGC 1 cut(s) 499
BccI CCATC 1 cut(s) 605
BcoDI GTCTC 2 cut(s) 181, 186
BfaI CTAG 1 cut(s) 192
BisI GCNGC 1 cut(s) 488
BlsI GCNGC 1 cut(s) 489
BmgBI CACGTC 1 cut(s) 711
BmiI GGNNCC 1 cut(s) 456
BmsI GCATC 4 cut(s) 78, 316, 688, 710
BpiI GAAGAC 2 cut(s) 290, 875
Bpu14I TTCGAA 1 cut(s) 770
Bsa29I ATCGAT 1 cut(s) 724
BsaAI YACGTR 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 681
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 439
Bse1I ACTGG 2 cut(s) 121, 600
Bse3DI GCAATG 1 cut(s) 535
BseCI ATCGAT 1 cut(s) 724
BseDI CCNNGG 1 cut(s) 681
BseGI GGATG 3 cut(s) 560, 701, 817
BseLI CCNNNNNNNGG 2 cut(s) 166, 439
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 3 cut(s) 357, 749, 805
BseNI ACTGG 2 cut(s) 121, 600
BseXI GCAGC 1 cut(s) 499
BshFI GGCC 2 cut(s) 513, 680
BshVI ATCGAT 1 cut(s) 724
BslI CCNNNNNNNGG 2 cut(s) 166, 439
BsmAI GTCTC 2 cut(s) 181, 186
BsmBI CGTCTC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 2 cut(s) 513, 680
Bsp119I TTCGAA 1 cut(s) 770
Bsp1407I TGTACA 1 cut(s) 448
Bsp143I GATC 3 cut(s) 142, 156, 831
BspACI CCGC 1 cut(s) 67
BspANI GGCC 2 cut(s) 513, 680
BspCNI CTCAG 3 cut(s) 358, 748, 804
BspDI ATCGAT 1 cut(s) 724
BspLI GGNNCC 1 cut(s) 456
BspPI GGATC 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 535
BsrGI TGTACA 1 cut(s) 448
BsrI ACTGG 2 cut(s) 121, 600
BssECI CCNNGG 1 cut(s) 681
BssMI GATC 3 cut(s) 142, 156, 831
BssNAI GTATAC 1 cut(s) 756
BssSI CACGAG 1 cut(s) 880
BssT1I CCWWGG 1 cut(s) 681
Bst1107I GTATAC 1 cut(s) 756
Bst2BI CACGAG 1 cut(s) 880
Bst4CI ACNGT 3 cut(s) 32, 250, 334
Bst6I CTCTTC 3 cut(s) 95, 392, 615
BstAUI TGTACA 1 cut(s) 448
BstBAI YACGTR 1 cut(s) 525
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 4 cut(s) 366, 473, 735, 791
BstENI CCTNNNNNAGG 2 cut(s) 164, 437
BstF5I GGATG 3 cut(s) 560, 701, 817
BstKTI GATC 3 cut(s) 145, 159, 834
BstMAI GTCTC 2 cut(s) 181, 186
BstMBI GATC 3 cut(s) 142, 156, 831
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 2 cut(s) 290, 875
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 1 cut(s) 156
BstZ17I GTATAC 1 cut(s) 756
Bsu15I ATCGAT 1 cut(s) 724
BsuRI GGCC 2 cut(s) 513, 680
BsuTUI ATCGAT 1 cut(s) 724
BtrI CACGTC 1 cut(s) 711
BtsCI GGATG 3 cut(s) 560, 701, 817
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 178, 399
Cac8I GCNNGC 1 cut(s) 390
ClaI ATCGAT 1 cut(s) 724
Csp6I GTAC 6 cut(s) 19, 33, 251, 449, 522, 568
CviQI GTAC 6 cut(s) 19, 33, 251, 449, 522, 568
DdeI CTNAG 4 cut(s) 366, 473, 735, 791
DpnI GATC 3 cut(s) 144, 158, 833
DpnII GATC 3 cut(s) 142, 156, 831
DraI TTTAAA 1 cut(s) 40
EaeI YGGCCR 2 cut(s) 511, 678
Eam1104I CTCTTC 3 cut(s) 95, 392, 615
EarI CTCTTC 3 cut(s) 95, 392, 615
Eco130I CCWWGG 1 cut(s) 681
Eco57I CTGAAG 1 cut(s) 98
EcoNI CCTNNNNNAGG 2 cut(s) 164, 437
EcoT14I CCWWGG 1 cut(s) 681
EcoT22I ATGCAT 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 186
FauNDI CATATG 2 cut(s) 49, 88
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 1 cut(s) 488
FokI GGATG 3 cut(s) 547, 688, 824
Fsp4HI GCNGC 1 cut(s) 488
FspBI CTAG 1 cut(s) 192
GluI GCNGC 1 cut(s) 488
HaeIII GGCC 2 cut(s) 513, 680
HinfI GANTC 4 cut(s) 404, 647, 733, 739
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 671, 732, 756
Hpy188I TCNGA 8 cut(s) 78, 349, 413, 565, 715, 738, 794, 804
Hpy188III TCNNGA 3 cut(s) 440, 617, 743
Hpy8I GTNNAC 3 cut(s) 671, 732, 756
HpyAV CCTTC 6 cut(s) 73, 143, 475, 593, 626, 799
HpyCH4III ACNGT 3 cut(s) 32, 250, 334
HpyCH4IV ACGT 3 cut(s) 524, 710, 758
HpyCH4V TGCA 8 cut(s) 86, 203, 224, 528, 701, 845, 876, 895
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 4 cut(s) 366, 473, 735, 791
HpySE526I ACGT 3 cut(s) 524, 710, 758
Kzo9I GATC 3 cut(s) 142, 156, 831
LmnI GCTCC 1 cut(s) 484
LpnPI CCDG 9 cut(s) 102, 330, 374, 425, 437, 527, 581, 602, 623
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 4 cut(s) 78, 316, 688, 710
MaeI CTAG 1 cut(s) 192
MaeII ACGT 3 cut(s) 524, 710, 758
MaeIII GTNAC 2 cut(s) 4, 361
MalI GATC 3 cut(s) 144, 158, 833
MboI GATC 3 cut(s) 142, 156, 831
MboII GAAGA 6 cut(s) 112, 290, 349, 409, 632, 880
MflI RGATCY 1 cut(s) 156
MlsI TGGCCA 2 cut(s) 513, 680
MluCI AATT 9 cut(s) 11, 213, 226, 241, 269, 381, 444, 517, 658
MluNI TGGCCA 2 cut(s) 513, 680
MlyI GAGTC 2 cut(s) 413, 727
MmeI TCCRAC 1 cut(s) 609
MnlI CCTC 5 cut(s) 160, 170, 312, 716, 802
Mox20I TGGCCA 2 cut(s) 513, 680
Mph1103I ATGCAT 1 cut(s) 703
MscI TGGCCA 2 cut(s) 513, 680
MseI TTAA 3 cut(s) 39, 465, 907
Msp20I TGGCCA 2 cut(s) 513, 680
MspA1I CMGCKG 1 cut(s) 148
Mva1269I GAATGC 1 cut(s) 798
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeI CATATG 2 cut(s) 49, 88
NdeII GATC 3 cut(s) 142, 156, 831
NlaIV GGNNCC 1 cut(s) 456
NmuCI GTSAC 2 cut(s) 4, 361
NsiI ATGCAT 1 cut(s) 703
NspV TTCGAA 1 cut(s) 770
PctI GAATGC 1 cut(s) 798
PfeI GAWTC 2 cut(s) 647, 739
PkrI GCNGC 1 cut(s) 489
PleI GAGTC 2 cut(s) 412, 727
PpsI GAGTC 2 cut(s) 412, 727
Ppu21I YACGTR 1 cut(s) 525
PsiI TTATAA 1 cut(s) 663
PspN4I GGNNCC 1 cut(s) 456
PsuI RGATCY 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 6 cut(s) 20, 34, 252, 450, 523, 569
RsaNI GTAC 6 cut(s) 19, 33, 251, 449, 522, 568
SaqAI TTAA 3 cut(s) 39, 465, 907
SatI GCNGC 1 cut(s) 488
Sau3AI GATC 3 cut(s) 142, 156, 831
SchI GAGTC 2 cut(s) 413, 727
SfaNI GCATC 4 cut(s) 78, 316, 688, 710
SfuI TTCGAA 1 cut(s) 770
Sse9I AATT 9 cut(s) 11, 213, 226, 241, 269, 381, 444, 517, 658
SsiI CCGC 1 cut(s) 67
SspMI CTAG 1 cut(s) 192
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 3 cut(s) 32, 250, 334
TaiI ACGT 3 cut(s) 527, 713, 761
TaqI TCGA 3 cut(s) 27, 724, 770
TasI AATT 9 cut(s) 11, 213, 226, 241, 269, 381, 444, 517, 658
TatI WGTACW 3 cut(s) 32, 448, 567
TfiI GAWTC 2 cut(s) 647, 739
Tru1I TTAA 3 cut(s) 39, 465, 907
Tru9I TTAA 3 cut(s) 39, 465, 907
TscAI CASTG 2 cut(s) 178, 399
TseFI GTSAC 2 cut(s) 4, 361
TseI GCWGC 1 cut(s) 487
Tsp45I GTSAC 2 cut(s) 4, 361
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 2 cut(s) 178, 399
XagI CCTNNNNNAGG 2 cut(s) 164, 437
XapI RAATTY 3 cut(s) 11, 226, 241
XmiI GTMKAC 1 cut(s) 755
XspI CTAG 1 cut(s) 192
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.