Rorug05G0532500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006053
Physical Location & Seq
Forward (+)
72070733 .. 72072021
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0532500.1

Sequence Viewer

Length: 513 bp
ATGAGGACTGGGTACTTGGTTTGTTTGTCTTTGGGGATTTGCCTTCTACTTCAAGTTCAACATGCTCATGCCAAAGAAAATAAAACGGACCCAACGGATAATGTAAATCTGACCCCATTCCGGGCTTGGAGAAGTGCTTATTACTGCTTGCAGAGAAAAGCCCCTGCTAATTGCACAGCATTTGAGACGGTTTCTCTGACAGACAGAGGACTAATAGATGTGATCCCAAGTGGGATAGGAGAATACTGCAGTGCAGACACAGGGTGTGCACAACACGTCTTGGATGTGCTTAAATGCATCTACCTTTGCAAACGAGACTTCTGGTTTGCCAATAACGCCACCGTTTCAGTGCTCAATCAAACCATTCATGATGGGTGCTCCAACAAAAAAAGTATATCGACACTGAATCAGAATTATAAAAAGAGCAGTGGGATGAAGGTGCAGCAGAAGATTTATATACCATTTGTTGCATCACTATCAACCTTGGCATTCATAGCCATCTTCAACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

18.91

Weight (kDa)

8.86

Isoelectric Point (pI)

25.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 39 - 137 1.9e-22 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AclWI GGATC 1 cut(s) 217
AdeI CACNNNGTG 1 cut(s) 264
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 2 cut(s) 120, 121
AflIII ACRYGT 2 cut(s) 274, 507
AgsI TTSAA 3 cut(s) 53, 59, 505
AjiI CACGTC 1 cut(s) 277
Alw21I GWGCWC 3 cut(s) 271, 354, 380
Alw26I GTCTC 2 cut(s) 179, 309
Alw44I GTGCAC 1 cut(s) 267
AlwI GGATC 1 cut(s) 217
ApaLI GTGCAC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 442
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 271
Bbv12I GWGCWC 3 cut(s) 271, 354, 380
BbvI GCAGC 1 cut(s) 454
BccI CCATC 2 cut(s) 365, 506
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 2 cut(s) 179, 309
BfmI CTRYAG 1 cut(s) 247
BisI GCNGC 1 cut(s) 443
BlsI GCNGC 1 cut(s) 444
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 88
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 122
BmrI ACTGGG 1 cut(s) 18
BmsI GCATC 2 cut(s) 306, 479
BmuI ACTGGG 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 483
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 121
Bse1I ACTGG 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 2 cut(s) 289, 438
BseLI CCNNNNNNNGG 2 cut(s) 120, 121
BseNI ACTGG 1 cut(s) 13
BseSI GKGCMC 1 cut(s) 271
BseXI GCAGC 1 cut(s) 454
BsgI GTGCAG 2 cut(s) 273, 461
BsiHKAI GWGCWC 3 cut(s) 271, 354, 380
BsiSI CCGG 1 cut(s) 121
BslI CCNNNNNNNGG 2 cut(s) 120, 121
BsmAI GTCTC 2 cut(s) 179, 309
BsmBI CGTCTC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 271, 354, 380
Bsp143I GATC 1 cut(s) 222
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 90
BspMAI CTGCAG 1 cut(s) 251
BspPI GGATC 1 cut(s) 217
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 190, 343
BstC8I GCNNGC 1 cut(s) 149
BstF5I GGATG 2 cut(s) 289, 438
BstKTI GATC 1 cut(s) 225
BstMAI GTCTC 2 cut(s) 179, 309
BstMBI GATC 1 cut(s) 222
BstMWI GCNNNNNNNGC 2 cut(s) 335, 494
BstNSI RCATGY 2 cut(s) 65, 511
BstSCI CCNGG 1 cut(s) 120
BstSFI CTRYAG 1 cut(s) 247
BstSLI GKGCMC 1 cut(s) 271
BstV1I GCAGC 1 cut(s) 454
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 2 cut(s) 289, 438
BtsI GCAGTG 2 cut(s) 256, 433
BtsIMutI CAGTG 4 cut(s) 256, 354, 401, 433
Cac8I GCNNGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 13
CviAII CATG 4 cut(s) 62, 68, 368, 508
CviJI RGCY 3 cut(s) 125, 161, 497
CviKI_1 RGCY 3 cut(s) 125, 161, 497
CviQI GTAC 1 cut(s) 13
DpnI GATC 1 cut(s) 224
DpnII GATC 1 cut(s) 222
DraIII CACNNNGTG 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 483
EcoT22I ATGCAT 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 4 cut(s) 65, 71, 371, 511
FaiI YATR 9 cut(s) 63, 69, 369, 395, 417, 456, 458, 494, 509
FatI CATG 4 cut(s) 61, 67, 367, 507
Fnu4HI GCNGC 1 cut(s) 443
FokI GGATG 2 cut(s) 296, 445
Fsp4HI GCNGC 1 cut(s) 443
GluI GCNGC 1 cut(s) 443
HapII CCGG 1 cut(s) 121
Hin1II CATG 4 cut(s) 65, 71, 371, 511
HinfI GANTC 1 cut(s) 406
HpaII CCGG 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 3 cut(s) 111, 198, 411
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 53, 430
HpyCH4III ACNGT 2 cut(s) 190, 343
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 9 cut(s) 151, 174, 249, 254, 269, 297, 309, 442, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 494
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 4 cut(s) 65, 71, 371, 511
Kzo9I GATC 1 cut(s) 222
LmnI GCTCC 1 cut(s) 383
LpnPI CCDG 4 cut(s) 134, 177, 246, 307
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 2 cut(s) 306, 479
MaeII ACGT 1 cut(s) 276
MalI GATC 1 cut(s) 224
MboI GATC 1 cut(s) 222
MboII GAAGA 2 cut(s) 460, 493
MhlI GDGCHC 3 cut(s) 271, 354, 380
MluCI AATT 2 cut(s) 169, 412
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 1 cut(s) 200
Mph1103I ATGCAT 1 cut(s) 299
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 66
MspI CCGG 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 488
MwoI GCNNNNNNNGC 2 cut(s) 335, 494
NciI CCSGG 1 cut(s) 122
NdeII GATC 1 cut(s) 222
NlaIII CATG 4 cut(s) 65, 71, 371, 511
NlaIV GGNNCC 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 299
NspI RCATGY 2 cut(s) 65, 511
PagI TCATGA 1 cut(s) 367
PciI ACATGT 1 cut(s) 507
PctI GAATGC 1 cut(s) 488
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 1 cut(s) 444
PscI ACATGT 1 cut(s) 507
PsiI TTATAA 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 88
PstI CTGCAG 1 cut(s) 251
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
RseI CAYNNNNRTG 1 cut(s) 66
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 1 cut(s) 443
Sau3AI GATC 1 cut(s) 222
Sau96I GGNCC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 3 cut(s) 271, 354, 380
SetI ASST 4 cut(s) 279, 306, 441, 485
SfaNI GCATC 2 cut(s) 306, 479
SfcI CTRYAG 1 cut(s) 247
SinI GGWCC 1 cut(s) 88
SmiMI CAYNNNNRTG 1 cut(s) 66
Sse9I AATT 2 cut(s) 169, 412
StyD4I CCNGG 1 cut(s) 120
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 190, 343
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 398
TasI AATT 2 cut(s) 169, 412
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 4 cut(s) 256, 354, 408, 433
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 3 cut(s) 356, 449, 481
TspGWI ACGGA 2 cut(s) 101, 110
TspRI CASTG 4 cut(s) 256, 354, 408, 433
VneI GTGCAC 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 88
XceI RCATGY 2 cut(s) 65, 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.