Rorug06G0022800

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0018188
Physical Location & Seq
Reverse (-)
2864342 .. 2868711
4370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0022800.1

Sequence Viewer

Length: 786 bp
ATGGCGGCCACGTCCCTTAGGGTTTTGCTCCTCGCTCTCCTCCTCATCGCTTCCCCCTTCCTCCAAGTCGCCAGGGGTGAGTCTGACTCTGACTCAGAATCAGATTCCGTATCTGAGGTTGTGGAAGAAAACAGTGATCTTGGGATTGTAGGCGATGATGTCCAGGATTTTGCGGATGCAAGTTTTAGTCCAGCACCAGGAATTGAGACAATCTGTGTTTTCCCCAAAAATAAAGCAAAAACGGTGGCGGCAGGGGAGCAGACTGAACTACTCGTTGGATTGAGAAATGATGGGGAGTCGAGCCTGAATGTGATTGCAGTCAAAGCCAGTGTTCATCTTCCATTTGATCACAACCTGCTGGTTCAGAATCTTACTGCAGTGGCTTTTAACAATGCATCAGTTCCAGCTTCAGCTCAGGCTACCTTTCCATACCTATTTGCTGTCAGCAAGTTCTTGCAGCCTGGAACCTTTGACCTAGTGGGTACCATTATTTATGAGATAGACCAGCAGCCATACCAAAGCACATACTACAATGGTACCATTGAGGTTGTTGAGGCTGGTAGTCTTCTCAGCATCGAGTCTGTTTTCCTTATAACCCTTGGATTTGCTCTTCTCGTCCTCTTGGGGTTATGGATTCACGGTCAAATACAGCACCTTTCTAAGAAAACCAAGAGGGCTCCCAAGGTGGAAGTTGGAACCAGGTCTACAGATGCCAACATGGATGAATGGCTTCAGGGAACAGCGTATACTCAGTCACTCAACAATAAGTCCAAGAAGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

261

Amino Acids

28.13

Weight (kDa)

4.85

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAP_alpha PF03896 78 - 244 2.5e-12 Translocon-associated protein (TRAP), alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018188)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0396941
rosa_laevigata RLG00000014123
rosa_roxburghii Rroxscaffold_5G00341930
rosa_rugosa Rorug06G0022800
rosa_samantha Rh4AG111700 Rh4AG111800 Rh4BG072000 Rh4DG069400 Rh6BG144400 Rh6DG128800
rosa_wichuraiana Rw4G006060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
Acc36I ACCTGC 1 cut(s) 363
Acc65I GGTACC 2 cut(s) 482, 536
AccB1I GGYRCC 2 cut(s) 482, 536
AccI GTMKAC 2 cut(s) 704, 746
AciI CCGC 3 cut(s) 5, 173, 248
AcoI YGGCCR 1 cut(s) 6
AcuI CTGAAG 2 cut(s) 393, 716
AfaI GTAC 2 cut(s) 484, 538
AfiI CCNNNNNNNGG 1 cut(s) 197
AjiI CACGTC 1 cut(s) 12
AjnI CCWGG 5 cut(s) 71, 162, 196, 460, 698
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 2 cut(s) 407, 413
AluI AGCT 2 cut(s) 407, 413
Alw26I GTCTC 1 cut(s) 200
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 457, 508
Asp700I GAANNNNTTC 1 cut(s) 729
Asp718I GGTACC 2 cut(s) 482, 536
AsuHPI GGTGA 1 cut(s) 89
AxyI CCTNAGG 1 cut(s) 17
BaeI ACNNNNGTAYC 2 cut(s) 520, 553
BanI GGYRCC 2 cut(s) 482, 536
BanII GRGCYC 1 cut(s) 679
BbsI GAAGAC 1 cut(s) 557
BbvI GCAGC 2 cut(s) 469, 520
BccI CCATC 1 cut(s) 284
BciT130I CCWGG 5 cut(s) 73, 164, 198, 462, 700
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 1 cut(s) 476
BfmI CTRYAG 2 cut(s) 375, 705
BfuAI ACCTGC 1 cut(s) 363
BisI GCNGC 4 cut(s) 6, 249, 458, 509
BlsI GCNGC 4 cut(s) 7, 250, 459, 510
Bme1390I CCNGG 5 cut(s) 73, 164, 198, 462, 700
BmgBI CACGTC 1 cut(s) 12
BmiI GGNNCC 5 cut(s) 466, 484, 538, 678, 697
BmrFI CCNGG 5 cut(s) 73, 164, 198, 462, 700
BmsI GCATC 4 cut(s) 166, 404, 582, 700
BpiI GAAGAC 1 cut(s) 557
BplI GAGNNNNNCTC 2 cut(s) 71, 103
Bpu10I CCTNAGC 1 cut(s) 414
BsaJI CCNNGG 3 cut(s) 72, 598, 681
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 327
Bse21I CCTNAGG 1 cut(s) 17
BseBI CCWGG 5 cut(s) 73, 164, 198, 462, 700
BseDI CCNNGG 3 cut(s) 72, 598, 681
BseGI GGATG 2 cut(s) 181, 727
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMII CTCAG 5 cut(s) 105, 108, 428, 583, 764
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 3 cut(s) 20, 29, 32
BseXI GCAGC 2 cut(s) 469, 520
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 2 cut(s) 482, 536
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 200
BsnI GGCC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 679
Bsp143I GATC 2 cut(s) 136, 346
BspACI CCGC 3 cut(s) 5, 173, 248
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 5 cut(s) 106, 107, 427, 582, 763
BspLI GGNNCC 5 cut(s) 466, 484, 538, 678, 697
BspMAI CTGCAG 1 cut(s) 379
BspMI ACCTGC 1 cut(s) 363
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 2 cut(s) 482, 536
BsrI ACTGG 1 cut(s) 327
BssECI CCNNGG 3 cut(s) 72, 598, 681
BssMI GATC 2 cut(s) 136, 346
BssNAI GTATAC 1 cut(s) 747
BssT1I CCWWGG 2 cut(s) 598, 681
Bst1107I GTATAC 1 cut(s) 747
Bst2UI CCWGG 5 cut(s) 73, 164, 198, 462, 700
Bst4CI ACNGT 3 cut(s) 134, 244, 641
Bst6I CTCTTC 1 cut(s) 615
BstDEI CTNAG 7 cut(s) 17, 94, 114, 414, 569, 660, 750
BstF5I GGATG 2 cut(s) 181, 727
BstKTI GATC 2 cut(s) 139, 349
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 2 cut(s) 136, 346
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 5 cut(s) 73, 164, 198, 462, 700
BstSCI CCNGG 5 cut(s) 71, 162, 196, 460, 698
BstSFI CTRYAG 2 cut(s) 375, 705
BstV1I GCAGC 2 cut(s) 469, 520
BstV2I GAAGAC 1 cut(s) 557
BstZ17I GTATAC 1 cut(s) 747
Bsu36I CCTNAGG 1 cut(s) 17
BsuRI GGCC 1 cut(s) 8
BtgZI GCGATG 2 cut(s) 31, 168
BtrI CACGTC 1 cut(s) 12
BtsCI GGATG 2 cut(s) 181, 727
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 3 cut(s) 139, 334, 384
BveI ACCTGC 1 cut(s) 363
CsiI ACCWGGT 1 cut(s) 698
Csp6I GTAC 2 cut(s) 483, 537
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 1 cut(s) 718
CviQI GTAC 2 cut(s) 483, 537
DdeI CTNAG 7 cut(s) 17, 94, 114, 414, 569, 660, 750
DpnI GATC 2 cut(s) 138, 348
DpnII GATC 2 cut(s) 136, 346
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 598, 681
Eco24I GRGCYC 1 cut(s) 679
Eco57I CTGAAG 2 cut(s) 393, 716
Eco81I CCTNAGG 1 cut(s) 17
EcoRII CCWGG 5 cut(s) 71, 162, 196, 460, 698
EcoT14I CCWWGG 2 cut(s) 598, 681
EcoT22I ATGCAT 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 679
ErhI CCWWGG 2 cut(s) 598, 681
FaeI CATG 1 cut(s) 721
FaiI YATR 8 cut(s) 430, 495, 514, 526, 593, 631, 719, 747
FatI CATG 1 cut(s) 717
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 2 cut(s) 704, 746
Fnu4HI GCNGC 4 cut(s) 6, 249, 458, 509
FokI GGATG 2 cut(s) 188, 734
FriOI GRGCYC 1 cut(s) 679
Fsp4HI GCNGC 4 cut(s) 6, 249, 458, 509
FspBI CTAG 1 cut(s) 476
GluI GCNGC 4 cut(s) 6, 249, 458, 509
HaeIII GGCC 1 cut(s) 8
Hin1II CATG 1 cut(s) 721
HinfI GANTC 9 cut(s) 80, 86, 92, 98, 104, 296, 367, 578, 634
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 2 cut(s) 705, 747
Hpy188I TCNGA 6 cut(s) 85, 91, 97, 103, 115, 366
Hpy8I GTNNAC 2 cut(s) 705, 747
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 3 cut(s) 134, 244, 641
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 5 cut(s) 179, 317, 377, 395, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 7 cut(s) 17, 94, 114, 414, 569, 660, 750
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 1 cut(s) 721
KpnI GGTACC 2 cut(s) 486, 540
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 2 cut(s) 136, 346
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 3 cut(s) 33, 256, 682
Lsp1109I GCAGC 2 cut(s) 469, 520
LweI GCATC 4 cut(s) 166, 404, 582, 700
MabI ACCWGGT 1 cut(s) 698
MaeI CTAG 1 cut(s) 476
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 753
MalI GATC 2 cut(s) 138, 348
MboI GATC 2 cut(s) 136, 346
MboII GAAGA 4 cut(s) 137, 329, 557, 602
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 1 cut(s) 201
MlyI GAGTC 5 cut(s) 80, 86, 89, 305, 587
MmeI TCCRAC 2 cut(s) 256, 673
MnlI CCTC 9 cut(s) 41, 50, 53, 71, 109, 538, 547, 629, 666
Mph1103I ATGCAT 1 cut(s) 397
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 1 cut(s) 387
MspR9I CCNGG 5 cut(s) 73, 164, 198, 462, 700
MvaI CCWGG 5 cut(s) 73, 164, 198, 462, 700
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeII GATC 2 cut(s) 136, 346
NlaIII CATG 1 cut(s) 721
NlaIV GGNNCC 5 cut(s) 466, 484, 538, 678, 697
NmuCI GTSAC 1 cut(s) 753
NsiI ATGCAT 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 615
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 4 cut(s) 98, 104, 367, 634
PfoI TCCNGGA 1 cut(s) 162
PkrI GCNGC 4 cut(s) 7, 250, 459, 510
PleI GAGTC 5 cut(s) 80, 86, 88, 304, 586
PpsI GAGTC 5 cut(s) 80, 86, 88, 304, 586
PsiI TTATAA 1 cut(s) 593
Psp6I CCWGG 5 cut(s) 71, 162, 196, 460, 698
PspGI CCWGG 5 cut(s) 71, 162, 196, 460, 698
PspN4I GGNNCC 5 cut(s) 466, 484, 538, 678, 697
PsrI GAACNNNNNNTAC 4 cut(s) 688, 720, 730, 762
PstI CTGCAG 1 cut(s) 379
RsaI GTAC 2 cut(s) 484, 538
RsaNI GTAC 2 cut(s) 483, 537
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 4 cut(s) 6, 249, 458, 509
Sau3AI GATC 2 cut(s) 136, 346
SchI GAGTC 5 cut(s) 80, 86, 89, 305, 587
ScrFI CCNGG 5 cut(s) 73, 164, 198, 462, 700
SduI GDGCHC 1 cut(s) 679
SexAI ACCWGGT 1 cut(s) 698
SfaNI GCATC 4 cut(s) 166, 404, 582, 700
SfcI CTRYAG 2 cut(s) 375, 705
Sse9I AATT 1 cut(s) 201
SsiI CCGC 3 cut(s) 5, 173, 248
SspMI CTAG 1 cut(s) 476
StyD4I CCNGG 5 cut(s) 71, 162, 196, 460, 698
StyI CCWWGG 2 cut(s) 598, 681
TaaI ACNGT 3 cut(s) 134, 244, 641
TaiI ACGT 1 cut(s) 14
TaqI TCGA 2 cut(s) 299, 576
TasI AATT 1 cut(s) 201
TauI GCSGC 2 cut(s) 8, 251
TfiI GAWTC 4 cut(s) 98, 104, 367, 634
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 3 cut(s) 139, 334, 384
TseFI GTSAC 1 cut(s) 753
TseI GCWGC 2 cut(s) 457, 508
Tsp45I GTSAC 1 cut(s) 753
TspDTI ATGAA 2 cut(s) 323, 738
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 3 cut(s) 139, 334, 384
XmiI GTMKAC 2 cut(s) 704, 746
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 1 cut(s) 476
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.