Rorug06G0032100

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0018667
Physical Location & Seq
Forward (+)
3844556 .. 3848743
4188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0032100.1

Sequence Viewer

Length: 3774 bp
ATGAGCACACAAAGAACCTCTACATCTTACCGCCCTTCAACTCCTCAGTGGAAATATGATGTCTTTCTGAGCTTTAGAGGTGTTGACACTCGCAAGGCTTTTACAGACCACTTATACACTGCATTGAAACATCAAGGAGTCATAACTTTTAGGGATGATCCTGAACTTCAAAAAGGGGAAGCTATTTCTCCAGAACTTTTTGCTGCAATTGAAGAATCGAGATTTGCTCTCATTGTTCTCTCACAAAATTATGCATCGTCAACATGGTGCTTGGATGAACTTGTGAGAATTCTTGAATGCATGAAAGCAAGAAAAACTGTGCTGCCAATTTTCTATGATGTTGATCCCTTTGTTGTACGAAAGCAAACAGGGAGTTTTAGAGAAGTCTTTGCTAATCATGAAGAAAGGTTTAGGGATGACAAAGAGAAAGTGAGAAGGTGGAGATATGCTTTAACCGAAGTGGCAAGTTTCTCAGGGTGGAATTCAAAGGAATGGTACGAATCAAAGCTGATAAGAGATATTATTGAAGTTATATGGAAAAAATTGCAACCTACATCATTCAGTTATGCAGAAAATCTAGTTGGAATTTATTCAAGATTGCAGCCAGTCAATTTGCTTTTAGATGTAGGGGTGGATGACGTCCGCTTCATTGGTATATGGGGAATGGGCGGGATTGGTAAGACTACTATGGTAAGAGCAGTGTATGAGAGAATCTCTCGTGAATTTGAATTTAGTTTCCTTCTTACTGATGTTAGAAACTTTGTTGAAAAAAGTGGTCTACTTAATTTACAAAAGCAACTTCTCTCTGGGATTTGGACAAAGAAGGCCAACATATCAGACCTTCATGAAGGAGCCATGATTATAAGGAGGTTGTTTGGTGACAAAAAAGTTCTTCTCATTCTTGATGATGTGAACCATTCAAGTCATTTAGAATATTTGGCAGGAAACCCAGAGTGGTTTGGTTCAGGGAGTAGAGTTCTTATCACAACTAGAAATGAGCATTTGTTGATTGGACATGGAGTGGAGAGAAGATTGAAGGTCGAAGAATTAAATGATGCTGATTCTCTTCAGGTTTTTAGCCGGAAAGCATTCAAAAGAGTTTACCCTGAAGGAGATTTTCTTGGTTTGTCAGAATATGTTATAAGTTATGCAAAAGGTGTTCCTTTAGCTCTTGAAGTACTCGGTTCTTTTTTGCATGGAAGAGATCTAAGTGAATGGAAAAGTGCACTGAGAAAACTGGGAAGAGTTTGTAACTTGGAAATTTTTGACATACTTAAAATAAGTTACAACGATCTAGATTCTGAAGAAAAGAAAATATTCCTAGACATTGCATGTTTCTTTAATGGACAGGACAAAGATCGAGTAACAGAAGTACTAAATAGTTGCGATTTTTCTGCAATTATTGGAATAAAAGTTCTCATGGAAAGATCCCTCCTAACTCTTTCAGATGGAAGACTATGGATGCATGATTTGCTCAGAGAAATGGGCCACGAAATTGTCCGCCTGGAATCTCCTACTGAGCAGGGTAGGTACAGTAGGTTGTGGCTTCTTGAAGATGTCAAACATGTCTTGACCAAATATACTGGAACTGAAGCAATAGAAGGCATATTTGTGGACTCAGCCGAATCAGGAATCGAGGTGGACGTGGCTCCGAAATCATTTTCAATGATGAACAAATTGAGATACCTGAAGATTAAGAATGGGAACCTGCCTAAGGGGCTTGAATATCTTCCCAATAGTTTACGGATTCTTGATTGGATGAGGTATCCGTCAAAATCTCTGCCATCACATTTCAACCCTCAGAAGCTGCTCGAACTTAGCTTGTGTCATAGTTGTATTAAACATGTTCAGATAGGAACTGAGCCTTTATACAATTTGAAAACCATTAATCTGAGTCACTCTCTGAATCTTGTCAACACCCCAAACTTTGAAGGTATGCCACATCTCAAGTTTCTGTTTCTTGAAGGTTGTATAAGATTGTATGAGGTTGACCCGACAATTGAAGTGCTTGAAAGACTTACTGTGCTGAACTTGAAAAATTGCAAGAATCTTGTGCATTTTGCAAGCAGTGTAGGTGGCTTAAAATCTCTCAAAGTTCTTAATCTTTCTGGTTGCTCAAAGCTTAAAAAACTACCGAATGACATGGGTCATTTAGAAAGTTTGGAGGAGCTTCATGTGAACGGCACCGCCATAAGAGAACTACCTTCCTCTATTGGAATGCTCGAAAGACTTCCTCTGCTGAAAATGAAAGATTGCAGAGATCTTGTGTCTCTTCCAATTAGTGTCCGTGGCTTAAAATCTCTCAAAGTTGTTAATATTTCTGGTTGCTCAAAGCTTGACACATTGCCAGAGGAGTTGGGCCATATAGAGTGTTTGGTAGAGGTTGATGTGAGTGGAACTTCTATACAAGAACTACCTTGCTCTATTGGGATGCTTGAAAGACTTGTTTCTATGTCCTTGAGAGATTGCAAACATCTTGTGTGTCTTCCAAGCAGTGTGGGTGGCTTAAAATCTCTCAAAGATCTCAATCTTTCTGGTTGCTCAAAGCTTGACAAATTGCCGGATGAGTTGGGTCATGTTGCACGTTTGGAGAAGCTTGATGTGAGTGGAAGTGGCATAAGAGAACTCCCCTCCTCTATTGGTCTTTTGAAGAACCTTAAACAGTTATCTCTTGCTAGATGTAAAGCGCAGGCCCCTAAATCATGGAATATGATGTTCAACCCCTTTCAGTTATTGCGAAAAAGAAGTCACATTCCGGCAGGATTGTCGTTGCCTTGTTTATCTGGTTTGCATTCATTAACCGAACTGGATCTAAGTGACTGCAATCTTTCTGAAGGAGCAATGCCCAGTGATTTTGGATGCTTGTCTTCATTAAGAGAATTAAATCTCAGCAGAAATCAATTCATTAGACTACCCGAGAGCATCGGCCAACTCTCTAGACTTGAAGTACTTGAGTTGAATTTGTGCGGCAAACTTCAGACATTACCAGAGTTTCTCCAAGAGAACAATGTATGGGTAGAGGCTAGCAACTGCATTTCATTAGATACATTGGCCAATCAAAGCGAACATTGCAATTCGGTACTAGAAGGATATTTTGTCAACTGTTTCAAAATGGTGGAGAATGAAAGCTGTAAGAGGATAGCAATTTCATTGCTAACACGCTTCCTGAAGTTTCAACATTCTATACATGATAGATTTGACTTTATTGCTCCTGGAAATGAAATACCAGAGTGGTACAATAACCAAAGTGAGGGGTCTTTGATAACTGTAGAGTTGCATCCAGGTTGGTTTAGTAACAAGTTTATGGGATTTGCCTTCTGTGTCGTTTTTAGACTCCTTAAACCACTCCCGCCTGGGGTTGAGATTGACATTAGTTGCGAACCAAGGGCCAATGGACAATACTTTGGTTCATCCCCTGTTCTATTGTTTAATAAAAAGTGGGGTCAACCTGTGTCAGATCACATTTGGTTCTTCTATGTGGGGTCAACCTGGGGTAGAGTCACTGAAGAATGGCAGGACATCTACTATGAGCTTGTATTCTCATTTGAATCATATGGAATCGATGAGGAAATTTTGCAAGTGAAGAAGTGTGGGGTCCGTATGGTATATGATGAAGATGCAAAGGAGCTTTGGGAAACATTGTTGAAGCAGAGCAATGTTAGCATTAATACCAAGCGAGGCCTTCAACACTATGATGATGATGATGCATCATCTAGTAGTACAAGTCAGGCTCATCCAAAAAGAATGAAACAACTCCACCTTGATGGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

1257

Amino Acids

142.88

Weight (kDa)

6.46

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 193 2.5e-52 TIR domain
TIR_2 PF13676 21 - 127 6.1e-15 TIR domain
NB-ARC PF00931 212 - 366 3.3e-18 NB-ARC domain
WHD_ROQ1 PF23282 435 - 503 1.8e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 665 - 773 3e-14 Leucine-rich repeat region
LRR_13 PF23286 694 - 779 5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 765 - 850 1.9e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 777 - 846 4.7e-09 Leucine-rich repeat region
LRR_14 PF23598 832 - 900 5.7e-09 Leucine-rich repeat region
LRR_13 PF23286 836 - 1021 7e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 923 - 997 3.3e-08 Leucine-rich repeat region
C-JID PF20160 1071 - 1207 6.1e-18 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018667)

Species Orthologous Gene IDs
rosa_laevigata RLG00000021160 RLG00000027272
rosa_multiflora Rmu_sc0001694.1_g000011 Rmu_sc0012684.1_g000003
rosa_rugosa Rorug01G0091500 Rorug06G0032100
rosa_samantha Rh5AG416100 Rh5DG340000 Rh7AG456400
rosa_wichuraiana Rw2G017880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 863, 1142
AatII GACGTC 1 cut(s) 642
Acc36I ACCTGC 1 cut(s) 1716
AccB1I GGYRCC 1 cut(s) 2183
AccI GTMKAC 1 cut(s) 778
AciI CCGC 7 cut(s) 31, 643, 669, 1501, 2187, 2967, 3350
AclWI GGATC 4 cut(s) 152, 338, 1422, 2817
AcoI YGGCCR 2 cut(s) 2926, 3051
AcsI RAATTY 8 cut(s) 288, 481, 585, 722, 728, 1260, 2959, 3570
AcuI CTGAAG 9 cut(s) 1052, 1128, 1323, 1611, 1709, 2853, 2960, 3188, 3525
AcyI GRCGYC 1 cut(s) 639
AfaI GTAC 9 cut(s) 357, 497, 1179, 1374, 1532, 2949, 3081, 3236, 3721
AfiI CCNNNNNNNGG 3 cut(s) 1714, 3250, 3355
AflIII ACRYGT 2 cut(s) 1564, 1843
AjiI CACGTC 1 cut(s) 1645
AjnI CCWGG 5 cut(s) 1503, 3211, 3280, 3352, 3488
AjuI GAANNNNNNNTTGG 2 cut(s) 564, 596
AloI GAACNNNNNNTCC 2 cut(s) 1398, 1430
Alw21I GWGCWC 2 cut(s) 8, 1228
Alw26I GTCTC 1 cut(s) 2274
Alw44I GTGCAC 1 cut(s) 1224
AlwI GGATC 4 cut(s) 152, 338, 1422, 2817
AlwNI CAGNNNCTG 1 cut(s) 1807
Ama87I CYCGRG 1 cut(s) 2915
AoxI GGCC 8 cut(s) 825, 1486, 2359, 2691, 2926, 3051, 3387, 3679
ApaLI GTGCAC 1 cut(s) 1224
ApeKI GCWGC 4 cut(s) 203, 322, 601, 1807
ApoI RAATTY 8 cut(s) 288, 481, 585, 722, 728, 1260, 2959, 3570
ArsI GACNNNNNNTTYG 2 cut(s) 2731, 2763
AseI ATTAAT 2 cut(s) 1887, 3666
Asp700I GAANNNNTTC 5 cut(s) 589, 1088, 1316, 1728, 2229
AspLEI GCGC 1 cut(s) 2689
AspS9I GGNCC 5 cut(s) 1486, 2359, 2692, 3387, 3595
AsuHPI GGTGA 1 cut(s) 890
AsuNHI GCTAGC 1 cut(s) 3023
AvaI CYCGRG 1 cut(s) 2915
AvaII GGWCC 1 cut(s) 3595
AxyI CCTNAGG 1 cut(s) 1713
BaeGI GKGCMC 1 cut(s) 1228
BalI TGGCCA 1 cut(s) 3053
BanI GGYRCC 1 cut(s) 2183
BauI CACGAG 1 cut(s) 717
BbsI GAAGAC 3 cut(s) 1459, 2477, 2859
Bbv12I GWGCWC 2 cut(s) 8, 1228
BbvI GCAGC 4 cut(s) 190, 309, 613, 1794
BccI CCATC 3 cut(s) 1442, 1792, 3758
BceAI ACGGC 1 cut(s) 2197
BcgI CGANNNNNNTGC 4 cut(s) 1376, 1410, 2748, 2782
BciT130I CCWGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
BciVI GTATCC 1 cut(s) 1776
BcoDI GTCTC 1 cut(s) 2274
BfaI CTAG 9 cut(s) 578, 990, 1295, 1322, 2676, 2937, 3024, 3083, 3714
BfmI CTRYAG 1 cut(s) 3267
BfuAI ACCTGC 1 cut(s) 1716
BfuI GTATCC 1 cut(s) 1776
BglI GCCNNNNNGGC 1 cut(s) 1717
BglII AGATCT 3 cut(s) 1204, 2260, 2521
BisI GCNGC 5 cut(s) 204, 323, 602, 1808, 2968
BlsI GCNGC 5 cut(s) 205, 324, 603, 1809, 2969
BmcAI AGTACT 3 cut(s) 1179, 1374, 2949
Bme1390I CCNGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
Bme18I GGWCC 1 cut(s) 3595
BmeT110I CYCGRG 1 cut(s) 2915
BmgBI CACGTC 1 cut(s) 1645
BmgT120I GGNCC 5 cut(s) 1486, 2359, 2692, 3387, 3595
BmiI GGNNCC 6 cut(s) 853, 1650, 1706, 2185, 2694, 3596
BmrFI CCNGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
BmrI ACTGGG 2 cut(s) 1247, 2841
BmtI GCTAGC 1 cut(s) 3027
BmuI ACTGGG 2 cut(s) 1247, 2841
BoxI GACNNNNGTC 1 cut(s) 2145
BpiI GAAGAC 3 cut(s) 1459, 2477, 2859
BplI GAGNNNNNCTC 8 cut(s) 211, 243, 698, 730, 700, 732, 1885, 1917
BpmI CTGGAG 1 cut(s) 174
BpuEI CTTGAG 3 cut(s) 1931, 2479, 2972
Bsa29I ATCGAT 1 cut(s) 3561
BsaBI GATNNNNATC 2 cut(s) 342, 2526
BsaHI GRCGYC 1 cut(s) 639
BsaJI CCNNGG 4 cut(s) 2287, 3353, 3383, 3489
Bsc4I CCNNNNNNNGG 3 cut(s) 1714, 3250, 3355
Bse1I ACTGG 5 cut(s) 605, 1242, 1588, 2811, 2847
Bse21I CCTNAGG 1 cut(s) 1713
Bse3DI GCAATG 6 cut(s) 1326, 2342, 2847, 3067, 3149, 3661
Bse8I GATNNNNATC 2 cut(s) 342, 2526
BseBI CCWGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
BseCI ATCGAT 1 cut(s) 3561
BseDI CCNNGG 4 cut(s) 2287, 3353, 3383, 3489
BseJI GATNNNNATC 2 cut(s) 342, 2526
BseLI CCNNNNNNNGG 3 cut(s) 1714, 3250, 3355
BseMI GCAATG 6 cut(s) 1326, 2342, 2847, 3067, 3149, 3661
BseNI ACTGG 5 cut(s) 605, 1242, 1588, 2811, 2847
BseRI GAGGAG 4 cut(s) 33, 2180, 2366, 2623
BseSI GKGCMC 1 cut(s) 1228
BseXI GCAGC 4 cut(s) 190, 309, 613, 1794
BshFI GGCC 8 cut(s) 827, 1488, 2361, 2693, 2928, 3053, 3389, 3681
BshNI GGYRCC 1 cut(s) 2183
BshVI ATCGAT 1 cut(s) 3561
BsiHKAI GWGCWC 2 cut(s) 8, 1228
BsiHKCI CYCGRG 1 cut(s) 2915
BsiSI CCGG 3 cut(s) 1081, 2561, 2756
BslI CCNNNNNNNGG 3 cut(s) 1714, 3250, 3355
BsmAI GTCTC 1 cut(s) 2274
BsmI GAATGC 4 cut(s) 302, 1088, 2223, 2791
BsnI GGCC 8 cut(s) 827, 1488, 2361, 2693, 2928, 3053, 3389, 3681
BsoBI CYCGRG 1 cut(s) 2915
Bsp1286I GDGCHC 2 cut(s) 8, 1228
BspACI CCGC 7 cut(s) 31, 643, 669, 1501, 2187, 2967, 3350
BspANI GGCC 8 cut(s) 827, 1488, 2361, 2693, 2928, 3053, 3389, 3681
BspDI ATCGAT 1 cut(s) 3561
BspHI TCATGA 2 cut(s) 397, 844
BspLI GGNNCC 6 cut(s) 853, 1650, 1706, 2185, 2694, 3596
BspMI ACCTGC 1 cut(s) 1716
BspOI GCTAGC 1 cut(s) 3027
BspPI GGATC 4 cut(s) 152, 338, 1422, 2817
BspT107I GGYRCC 1 cut(s) 2183
BsrDI GCAATG 6 cut(s) 1326, 2342, 2847, 3067, 3149, 3661
BsrI ACTGG 5 cut(s) 605, 1242, 1588, 2811, 2847
BssECI CCNNGG 4 cut(s) 2287, 3353, 3383, 3489
BssNI GRCGYC 1 cut(s) 639
BssSI CACGAG 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 3383
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
Bst4CI ACNGT 6 cut(s) 319, 1535, 2023, 2664, 3104, 3268
Bst6I CTCTTC 4 cut(s) 1071, 1195, 1237, 2277
BstACI GRCGYC 1 cut(s) 639
BstAPI GCANNNNNTGC 1 cut(s) 1471
BstC8I GCNNGC 3 cut(s) 2065, 2691, 3025
BstDSI CCRYGG 1 cut(s) 2287
BstENI CCTNNNNNAGG 1 cut(s) 1712
BstHHI GCGC 1 cut(s) 2689
BstMAI GTCTC 1 cut(s) 2274
BstMWI GCNNNNNNNGC 4 cut(s) 1471, 1717, 3069, 3660
BstNI CCWGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
BstNSI RCATGY 3 cut(s) 1335, 1568, 1847
BstPAI GACNNNNGTC 1 cut(s) 2145
BstSCI CCNGG 5 cut(s) 1503, 3211, 3280, 3352, 3488
BstSFI CTRYAG 1 cut(s) 3267
BstSLI GKGCMC 1 cut(s) 1228
BstV1I GCAGC 4 cut(s) 190, 309, 613, 1794
BstV2I GAAGAC 3 cut(s) 1459, 2477, 2859
BstX2I RGATCY 5 cut(s) 1204, 1427, 2260, 2521, 2809
BstXI CCANNNNNNTGG 1 cut(s) 3764
BstYI RGATCY 5 cut(s) 1204, 1427, 2260, 2521, 2809
Bsu15I ATCGAT 1 cut(s) 3561
Bsu36I CCTNAGG 1 cut(s) 1713
BsuI GTATCC 1 cut(s) 1776
BsuRI GGCC 8 cut(s) 827, 1488, 2361, 2693, 2928, 3053, 3389, 3681
BsuTUI ATCGAT 1 cut(s) 3561
BtgI CCRYGG 1 cut(s) 2287
BtrI CACGTC 1 cut(s) 1645
BtsI GCAGTG 4 cut(s) 117, 705, 2074, 2500
BtsIMutI CAGTG 8 cut(s) 53, 117, 705, 1226, 2074, 2500, 2854, 3501
BveI ACCTGC 1 cut(s) 1716
Cac8I GCNNGC 3 cut(s) 2065, 2691, 3025
CaiI CAGNNNCTG 1 cut(s) 1807
CciI TCATGA 2 cut(s) 397, 844
CfoI GCGC 1 cut(s) 2689
Cfr13I GGNCC 5 cut(s) 1486, 2359, 2692, 3387, 3595
ClaI ATCGAT 1 cut(s) 3561
Csp6I GTAC 9 cut(s) 356, 496, 1178, 1373, 1531, 2948, 3080, 3235, 3720
CspCI CAANNNNNGTGG 2 cut(s) 2478, 2513
CviQI GTAC 9 cut(s) 356, 496, 1178, 1373, 1531, 2948, 3080, 3235, 3720
EaeI YGGCCR 2 cut(s) 2926, 3051
Eam1104I CTCTTC 4 cut(s) 1071, 1195, 1237, 2277
EarI CTCTTC 4 cut(s) 1071, 1195, 1237, 2277
EciI GGCGGA 1 cut(s) 1490
Eco130I CCWWGG 1 cut(s) 3383
Eco147I AGGCCT 1 cut(s) 3681
Eco47I GGWCC 1 cut(s) 3595
Eco57I CTGAAG 9 cut(s) 1052, 1128, 1323, 1611, 1709, 2853, 2960, 3188, 3525
Eco81I CCTNAGG 1 cut(s) 1713
Eco88I CYCGRG 1 cut(s) 2915
EcoNI CCTNNNNNAGG 1 cut(s) 1712
EcoO109I RGGNCCY 1 cut(s) 2692
EcoRI GAATTC 2 cut(s) 288, 481
EcoRII CCWGG 5 cut(s) 1503, 3211, 3280, 3352, 3488
EcoT14I CCWWGG 1 cut(s) 3383
EcoT22I ATGCAT 4 cut(s) 256, 302, 1467, 3709
ErhI CCWWGG 1 cut(s) 3383
FauI CCCGC 2 cut(s) 662, 3357
FauNDI CATATG 1 cut(s) 3553
FblI GTMKAC 1 cut(s) 778
Fnu4HI GCNGC 5 cut(s) 204, 323, 602, 1808, 2968
Fsp4HI GCNGC 5 cut(s) 204, 323, 602, 1808, 2968
FspBI CTAG 9 cut(s) 578, 990, 1295, 1322, 2676, 2937, 3024, 3083, 3714
GlaI GCGC 1 cut(s) 2688
GluI GCNGC 5 cut(s) 204, 323, 602, 1808, 2968
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 8 cut(s) 827, 1488, 2361, 2693, 2928, 3053, 3389, 3681
HapII CCGG 3 cut(s) 1081, 2561, 2756
HhaI GCGC 1 cut(s) 2689
Hin1I GRCGYC 1 cut(s) 639
Hin6I GCGC 1 cut(s) 2687
HinP1I GCGC 1 cut(s) 2687
HincII GTYRAC 7 cut(s) 85, 261, 1915, 1990, 3100, 3446, 3486
HindII GTYRAC 7 cut(s) 85, 261, 1915, 1990, 3100, 3446, 3486
HindIII AAGCTT 4 cut(s) 2120, 2333, 2546, 2594
HpaII CCGG 3 cut(s) 1081, 2561, 2756
HphI GGTGA 1 cut(s) 890
HpyCH4III ACNGT 6 cut(s) 319, 1535, 2023, 2664, 3104, 3268
HpyCH4IV ACGT 3 cut(s) 639, 1644, 2584
HpyF10VI GCNNNNNNNGC 4 cut(s) 1471, 1717, 3069, 3660
HpySE526I ACGT 3 cut(s) 639, 1644, 2584
Hsp92I GRCGYC 1 cut(s) 639
HspAI GCGC 1 cut(s) 2687
LmnI GCTCC 6 cut(s) 851, 1654, 2167, 2837, 3214, 3625
Lsp1109I GCAGC 4 cut(s) 190, 309, 613, 1794
MaeI CTAG 9 cut(s) 578, 990, 1295, 1322, 2676, 2937, 3024, 3083, 3714
MaeII ACGT 3 cut(s) 639, 1644, 2584
MaeIII GTNAC 9 cut(s) 878, 1250, 1283, 1363, 1895, 2747, 2816, 3293, 3499
MfeI CAATTG 2 cut(s) 207, 1998
MflI RGATCY 5 cut(s) 1204, 1427, 2260, 2521, 2809
MhlI GDGCHC 2 cut(s) 8, 1228
MlsI TGGCCA 1 cut(s) 3053
MluNI TGGCCA 1 cut(s) 3053
MlyI GAGTC 5 cut(s) 147, 1610, 1903, 3327, 3507
MmeI TCCRAC 1 cut(s) 562
Mox20I TGGCCA 1 cut(s) 3053
Mph1103I ATGCAT 4 cut(s) 256, 302, 1467, 3709
MroXI GAANNNNTTC 5 cut(s) 589, 1088, 1316, 1728, 2229
MscI TGGCCA 1 cut(s) 3053
MslI CAYNNNNRTG 3 cut(s) 1610, 3693, 3762
Msp20I TGGCCA 1 cut(s) 3053
MspI CCGG 3 cut(s) 1081, 2561, 2756
MspR9I CCNGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
MunI CAATTG 2 cut(s) 207, 1998
Mva1269I GAATGC 4 cut(s) 302, 1088, 2223, 2791
MvaI CCWGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
MwoI GCNNNNNNNGC 4 cut(s) 1471, 1717, 3069, 3660
NdeI CATATG 1 cut(s) 3553
NheI GCTAGC 1 cut(s) 3023
NlaIV GGNNCC 6 cut(s) 853, 1650, 1706, 2185, 2694, 3596
NmuCI GTSAC 5 cut(s) 878, 1895, 2747, 2816, 3499
NsiI ATGCAT 4 cut(s) 256, 302, 1467, 3709
NspI RCATGY 3 cut(s) 1335, 1568, 1847
PagI TCATGA 2 cut(s) 397, 844
PceI AGGCCT 1 cut(s) 3681
PciI ACATGT 2 cut(s) 1564, 1843
PcsI WCGNNNNNNNCGW 1 cut(s) 1641
PctI GAATGC 4 cut(s) 302, 1088, 2223, 2791
PdmI GAANNNNTTC 5 cut(s) 589, 1088, 1316, 1728, 2229
PfoI TCCNGGA 1 cut(s) 3211
PkrI GCNGC 5 cut(s) 205, 324, 603, 1809, 2969
PleI GAGTC 5 cut(s) 146, 1610, 1902, 3327, 3506
PpsI GAGTC 5 cut(s) 146, 1610, 1902, 3327, 3506
PscI ACATGT 2 cut(s) 1564, 1843
PshAI GACNNNNGTC 1 cut(s) 2145
PshBI ATTAAT 2 cut(s) 1887, 3666
PsiI TTATAA 2 cut(s) 863, 1142
Psp6I CCWGG 5 cut(s) 1503, 3211, 3280, 3352, 3488
PspGI CCWGG 5 cut(s) 1503, 3211, 3280, 3352, 3488
PspN4I GGNNCC 6 cut(s) 853, 1650, 1706, 2185, 2694, 3596
PspPI GGNCC 5 cut(s) 1486, 2359, 2692, 3387, 3595
PstNI CAGNNNCTG 1 cut(s) 1807
PsuI RGATCY 5 cut(s) 1204, 1427, 2260, 2521, 2809
RsaI GTAC 9 cut(s) 357, 497, 1179, 1374, 1532, 2949, 3081, 3236, 3721
RsaNI GTAC 9 cut(s) 356, 496, 1178, 1373, 1531, 2948, 3080, 3235, 3720
RseI CAYNNNNRTG 3 cut(s) 1610, 3693, 3762
SatI GCNGC 5 cut(s) 204, 323, 602, 1808, 2968
Sau96I GGNCC 5 cut(s) 1486, 2359, 2692, 3387, 3595
ScaI AGTACT 3 cut(s) 1179, 1374, 2949
SchI GAGTC 5 cut(s) 147, 1610, 1903, 3327, 3507
ScrFI CCNGG 5 cut(s) 1505, 3213, 3282, 3354, 3490
SduI GDGCHC 2 cut(s) 8, 1228
SfcI CTRYAG 1 cut(s) 3267
SinI GGWCC 1 cut(s) 3595
SmiMI CAYNNNNRTG 3 cut(s) 1610, 3693, 3762
SmlI CTYRAG 3 cut(s) 1946, 2458, 2951
SmoI CTYRAG 3 cut(s) 1946, 2458, 2951
SseBI AGGCCT 1 cut(s) 3681
SsiI CCGC 7 cut(s) 31, 643, 669, 1501, 2187, 2967, 3350
SspI AATATT 3 cut(s) 935, 1317, 2317
SspMI CTAG 9 cut(s) 578, 990, 1295, 1322, 2676, 2937, 3024, 3083, 3714
StuI AGGCCT 1 cut(s) 3681
StyD4I CCNGG 5 cut(s) 1503, 3211, 3280, 3352, 3488
StyI CCWWGG 1 cut(s) 3383
TaaI ACNGT 6 cut(s) 319, 1535, 2023, 2664, 3104, 3268
TaiI ACGT 3 cut(s) 642, 1647, 2587
TaqI TCGA 7 cut(s) 218, 1041, 1360, 1635, 1812, 2223, 3561
TatI WGTACW 4 cut(s) 1177, 1372, 2947, 3719
TauI GCSGC 1 cut(s) 2970
TscAI CASTG 8 cut(s) 53, 124, 705, 1233, 2074, 2500, 2854, 3508
TseFI GTSAC 5 cut(s) 878, 1895, 2747, 2816, 3499
TseI GCWGC 4 cut(s) 203, 322, 601, 1807
Tsp45I GTSAC 5 cut(s) 878, 1895, 2747, 2816, 3499
TspGWI ACGGA 4 cut(s) 1758, 1759, 2276, 3587
TspRI CASTG 8 cut(s) 53, 124, 705, 1233, 2074, 2500, 2854, 3508
VneI GTGCAC 1 cut(s) 1224
VpaK11BI GGWCC 1 cut(s) 3595
VspI ATTAAT 2 cut(s) 1887, 3666
XagI CCTNNNNNAGG 1 cut(s) 1712
XapI RAATTY 8 cut(s) 288, 481, 585, 722, 728, 1260, 2959, 3570
XbaI TCTAGA 2 cut(s) 1294, 2936
XceI RCATGY 3 cut(s) 1335, 1568, 1847
XmiI GTMKAC 1 cut(s) 778
XmnI GAANNNNTTC 5 cut(s) 589, 1088, 1316, 1728, 2229
XspI CTAG 9 cut(s) 578, 990, 1295, 1322, 2676, 2937, 3024, 3083, 3714
ZraI GACGTC 1 cut(s) 640
ZrmI AGTACT 3 cut(s) 1179, 1374, 2949
Zsp2I ATGCAT 4 cut(s) 256, 302, 1467, 3709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.