Rorug06G0248800

Ubiquitin-fold modifier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013440
Physical Location & Seq
Forward (+)
39527029 .. 39533488
6460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0248800.1

Sequence Viewer

Length: 1335 bp
ATGAAGAATTTTCTCTGCGATTTTCGCTTCCTCCTACTTCTCGCAGCTGCCGCCTTCATCTATATCCAGGTTCGGCTTTTCGCGACGCAATCACAGTATGCTGATCGCCTCGCCGATGCGATAGAGTCGGAGAACCAGTGTACATCTCAGTTGAGATCACTAATTGATCAAATAAGCTTGCAACAAGGGCAAATTGAAACCATGAAAGAACAAAAGGAACAGCTAGAGCAAGAGTGTGGACAACTGAAGGCCCTTGTTCAAGAACTTGAAAGGAAAGGTGTTGAACGGTTGATTAACAAAGAACAGGTGCCAGTGGCAGCAGTTGTAATTATGGCATGCAATCGTGCTGACTATCTGGAAAGGACCATTAAGTCTGTATTAAAATACCAGAATTCTGTTGCCACGAAGTTTCCTCTATTTGTCTCCCAGGATGGATCTGATCCGGGTGTCAAAGATAAGGCTTTGAGCTATGAACAGCTCACGTATATGCAGCATTTGGATTATAAACCAGTTCAACCTGATAGACCAGGGGAATTGATTGCATACTACAAAATTGCAAATCATTACAAATGGGCACTGGATGGATTGTTCTACAAGCATAAGTTCAGCCGAGTAATCATACTCGAAGATGATATGGAAATTGCTCCCGATTTTTTTGATTACTTTGAGGCTGCCACCGATCTCCTTGATAAGGATAAGTCTATCATGGCTGTTTCCTCATGGAATGACAATGGACAAAAGCAGTTTGTGCATGATCCAGAAGCACTTTATAGATCTGATTTCTTTCCTGGACTTGGGTGGATGCTGACTAGATCTACATGGGATGAGCTATCACCGAAGTGGCCAAAGGCTTACTGGGATGACTGGTTGAGGTTACAAGAGAATCACAAAGGTCGACAATTTCTTCGTCCAGAAGTGTGCAGAACATATAACTTTGGGGAACATGGTTCTAGTCTGGGACAGTATTTCAAACAATATCTTGAGCCAATCAAGCTGAATGATGTCCCTGTTGACTGGAAATCAATGGATTTGAGCTACCTGATGGAGGACAAGTATATAAAGCATTTTGCTGAGATTGTGAAAAAGGCTAAACCTGTCCATGGAGCTGATCTTGTACTGAAGGCACGCAATATGGCTGGTGATGTTCGTATACAGTACAATGACCAGCAAGACTTTGAACGCATTGCACATGAGTTTGGAATTTTTGAAGAGTGGAAGGATGGTATACCAAGGACAGCCTACAAAGGAGTAGTTGTTTTCCGACACCAAAACCCACAACGTATATTCCTTGTTGGTCCAGGTTCTCTGAAGCAGCTTGGAGTTGATGATTCTTGA

Protein Analysis

444

Amino Acids

51.7

Weight (kDa)

5.78

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNT-I PF03071 15 - 442 5e-184 GNT-I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77710
fragaria_vesca FvH4_2g26390
malus_domestica MD08G1169400.v1.1 MD15G1356000.v1.1
prunus_persica Prupe.1G503300_v2.0.a1 Prupe.1G503300_v2.0.a1
pyrus_communis pycom08g14480 pycom15g31800
rosa_chinensis RchiOBHm_Chr6g0294951
rosa_multiflora Rmu_sc0002700.1_g000040
rosa_roxburghii Rroxscaffold_7G00172800
rosa_rugosa Rorug06G0248600 Rorug06G0248800
rosa_samantha Rh6AG361800 Rh6BG369300 Rh6CG375800 Rh6DG362300
rosa_wichuraiana Rw6G031560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AasI GACNNNNNNGTC 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 307
AccI GTMKAC 3 cut(s) 895, 1150, 1225
AccII CGCG 1 cut(s) 83
AciI CCGC 1 cut(s) 51
AclWI GGATC 3 cut(s) 434, 442, 749
AcoI YGGCCR 1 cut(s) 842
AcsI RAATTY 3 cut(s) 7, 391, 1200
AcuI CTGAAG 3 cut(s) 266, 1139, 1328
AfaI GTAC 3 cut(s) 142, 1116, 1157
AfiI CCNNNNNNNGG 4 cut(s) 691, 794, 1045, 1100
AgsI TTSAA 8 cut(s) 197, 260, 269, 284, 515, 970, 1178, 1208
AjnI CCWGG 5 cut(s) 66, 426, 526, 787, 1297
AleI CACNNNNGTG 1 cut(s) 838
AloI GAACNNNNNNTCC 2 cut(s) 572, 604
Alw26I GTCTC 1 cut(s) 427
AlwI GGATC 3 cut(s) 434, 442, 749
AoxI GGCC 2 cut(s) 249, 842
ApeKI GCWGC 6 cut(s) 44, 47, 317, 490, 671, 1312
ApoI RAATTY 3 cut(s) 7, 391, 1200
ArsI GACNNNNNNTTYG 2 cut(s) 888, 920
AspS9I GGNCC 3 cut(s) 250, 363, 1295
AsuC2I CCSGG 1 cut(s) 444
AsuHPI GGTGA 2 cut(s) 825, 1151
AvaII GGWCC 2 cut(s) 363, 1295
BaeGI GKGCMC 1 cut(s) 577
BalI TGGCCA 1 cut(s) 844
BanI GGYRCC 1 cut(s) 307
BbvI GCAGC 6 cut(s) 34, 56, 329, 502, 658, 1324
BccI CCATC 4 cut(s) 425, 575, 1036, 1214
BciT130I CCWGG 5 cut(s) 68, 428, 528, 789, 1299
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 1 cut(s) 444
BcoDI GTCTC 1 cut(s) 427
BfaI CTAG 3 cut(s) 224, 810, 951
BglII AGATCT 2 cut(s) 773, 812
BisI GCNGC 7 cut(s) 45, 48, 51, 318, 491, 672, 1313
BlsI GCNGC 7 cut(s) 46, 49, 52, 319, 492, 673, 1314
Bme1390I CCNGG 6 cut(s) 68, 428, 444, 528, 789, 1299
Bme18I GGWCC 2 cut(s) 363, 1295
BmgT120I GGNCC 3 cut(s) 250, 363, 1295
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 6 cut(s) 68, 428, 444, 528, 789, 1299
BmrI ACTGGG 1 cut(s) 865
BmsI GCATC 2 cut(s) 106, 792
BmuI ACTGGG 1 cut(s) 865
BpuEI CTTGAG 1 cut(s) 1001
BpuMI CCSGG 1 cut(s) 444
BsaAI YACGTR 1 cut(s) 483
BsaJI CCNNGG 4 cut(s) 426, 527, 1099, 1229
BsaXI ACNNNNNCTCC 6 cut(s) 122, 152, 1037, 1067, 1239, 1269
Bsc4I CCNNNNNNNGG 4 cut(s) 691, 794, 1045, 1100
Bse1I ACTGG 7 cut(s) 136, 311, 509, 582, 860, 869, 1019
Bse3DI GCAATG 1 cut(s) 1182
BseBI CCWGG 5 cut(s) 68, 428, 528, 789, 1299
BseDI CCNNGG 4 cut(s) 426, 527, 1099, 1229
BseGI GGATG 6 cut(s) 436, 586, 807, 829, 865, 1225
BseLI CCNNNNNNNGG 4 cut(s) 691, 794, 1045, 1100
BseMI GCAATG 1 cut(s) 1182
BseMII CTCAG 2 cut(s) 161, 1062
BseNI ACTGG 7 cut(s) 136, 311, 509, 582, 860, 869, 1019
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 6 cut(s) 34, 56, 329, 502, 658, 1324
BsgI GTGCAG 1 cut(s) 940
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 2 cut(s) 251, 844
BshNI GGYRCC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 443
BslFI GGGAC 2 cut(s) 972, 989
BslI CCNNNNNNNGG 4 cut(s) 691, 794, 1045, 1100
BsmAI GTCTC 1 cut(s) 427
BsmFI GGGAC 2 cut(s) 972, 989
BsnI GGCC 2 cut(s) 251, 844
Bsp1286I GDGCHC 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 140
Bsp19I CCATGG 1 cut(s) 1099
Bsp68I TCGCGA 1 cut(s) 83
BspACI CCGC 1 cut(s) 51
BspANI GGCC 2 cut(s) 251, 844
BspCNI CTCAG 2 cut(s) 160, 1063
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 3 cut(s) 434, 442, 749
BspT107I GGYRCC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 1182
BsrGI TGTACA 1 cut(s) 140
BsrI ACTGG 7 cut(s) 136, 311, 509, 582, 860, 869, 1019
BssECI CCNNGG 4 cut(s) 426, 527, 1099, 1229
BssNAI GTATAC 2 cut(s) 1151, 1226
BssT1I CCWWGG 2 cut(s) 1099, 1229
Bst1107I GTATAC 2 cut(s) 1151, 1226
Bst2UI CCWGG 5 cut(s) 68, 428, 528, 789, 1299
Bst4CI ACNGT 4 cut(s) 96, 288, 963, 1155
Bst6I CTCTTC 1 cut(s) 1203
BstAPI GCANNNNNTGC 1 cut(s) 748
BstAUI TGTACA 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 3 cut(s) 179, 337, 1126
BstDEI CTNAG 2 cut(s) 147, 1071
BstDSI CCRYGG 1 cut(s) 1099
BstENI CCTNNNNNAGG 2 cut(s) 689, 1043
BstF5I GGATG 6 cut(s) 436, 586, 807, 829, 865, 1225
BstFNI CGCG 1 cut(s) 83
BstMAI GTCTC 1 cut(s) 427
BstMWI GCNNNNNNNGC 5 cut(s) 24, 50, 187, 748, 991
BstNI CCWGG 5 cut(s) 68, 428, 528, 789, 1299
BstNSI RCATGY 1 cut(s) 339
BstSCI CCNGG 6 cut(s) 66, 426, 442, 526, 787, 1297
BstSLI GKGCMC 1 cut(s) 577
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 6 cut(s) 34, 56, 329, 502, 658, 1324
BstX2I RGATCY 3 cut(s) 434, 773, 812
BstYI RGATCY 3 cut(s) 434, 773, 812
BstZ17I GTATAC 2 cut(s) 1151, 1226
BsuRI GGCC 2 cut(s) 251, 844
BtgI CCRYGG 1 cut(s) 1099
BtsCI GGATG 6 cut(s) 436, 586, 807, 829, 865, 1225
BtsIMutI CAGTG 3 cut(s) 143, 318, 575
BtuMI TCGCGA 1 cut(s) 83
Cac8I GCNNGC 3 cut(s) 179, 337, 1126
Cfr13I GGNCC 3 cut(s) 250, 363, 1295
CseI GACGC 1 cut(s) 94
Csp6I GTAC 3 cut(s) 141, 1115, 1156
CviAII CATG 9 cut(s) 202, 336, 706, 720, 752, 819, 944, 1100, 1190
CviQI GTAC 3 cut(s) 141, 1115, 1156
DdeI CTNAG 2 cut(s) 147, 1071
DrdI GACNNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 1 cut(s) 842
Eam1104I CTCTTC 1 cut(s) 1203
EarI CTCTTC 1 cut(s) 1203
Eco130I CCWWGG 2 cut(s) 1099, 1229
Eco47I GGWCC 2 cut(s) 363, 1295
Eco57I CTGAAG 3 cut(s) 266, 1139, 1328
EcoNI CCTNNNNNAGG 2 cut(s) 689, 1043
EcoO109I RGGNCCY 1 cut(s) 250
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 5 cut(s) 66, 426, 526, 787, 1297
EcoT14I CCWWGG 2 cut(s) 1099, 1229
ErhI CCWWGG 2 cut(s) 1099, 1229
FaeI CATG 9 cut(s) 205, 339, 709, 723, 755, 822, 947, 1103, 1193
FaqI GGGAC 2 cut(s) 972, 989
FatI CATG 9 cut(s) 201, 335, 705, 719, 751, 818, 943, 1099, 1189
FbaI TGATCA 1 cut(s) 166
FblI GTMKAC 3 cut(s) 895, 1150, 1225
Fnu4HI GCNGC 7 cut(s) 45, 48, 51, 318, 491, 672, 1313
FokI GGATG 6 cut(s) 443, 593, 814, 836, 872, 1232
Fsp4HI GCNGC 7 cut(s) 45, 48, 51, 318, 491, 672, 1313
FspBI CTAG 3 cut(s) 224, 810, 951
GluI GCNGC 7 cut(s) 45, 48, 51, 318, 491, 672, 1313
HaeIII GGCC 2 cut(s) 251, 844
HapII CCGG 1 cut(s) 443
HgaI GACGC 1 cut(s) 94
Hin1II CATG 9 cut(s) 205, 339, 709, 723, 755, 822, 947, 1103, 1193
HincII GTYRAC 2 cut(s) 896, 1012
HindII GTYRAC 2 cut(s) 896, 1012
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 3 cut(s) 125, 883, 1328
HpaII CCGG 1 cut(s) 443
HphI GGTGA 2 cut(s) 825, 1151
Hpy166II GTNNAC 6 cut(s) 141, 239, 896, 1012, 1151, 1226
Hpy188I TCNGA 5 cut(s) 130, 439, 778, 1262, 1308
Hpy188III TCNNGA 8 cut(s) 82, 260, 356, 647, 758, 911, 980, 1332
Hpy8I GTNNAC 6 cut(s) 141, 239, 896, 1012, 1151, 1226
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 64, 241, 1114, 1210
HpyCH4III ACNGT 4 cut(s) 96, 288, 963, 1155
HpyCH4IV ACGT 2 cut(s) 482, 1279
HpyCH4V TGCA 8 cut(s) 181, 339, 490, 542, 557, 751, 921, 1187
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 50, 187, 748, 991
HpyF3I CTNAG 2 cut(s) 147, 1071
HpySE526I ACGT 2 cut(s) 482, 1279
Hsp92II CATG 9 cut(s) 205, 339, 709, 723, 755, 822, 947, 1103, 1193
Ksp22I TGATCA 1 cut(s) 166
LmnI GCTCC 2 cut(s) 649, 1103
Lsp1109I GCAGC 6 cut(s) 34, 56, 329, 502, 658, 1324
LweI GCATC 2 cut(s) 106, 792
MaeI CTAG 3 cut(s) 224, 810, 951
MaeII ACGT 2 cut(s) 482, 1279
MaeIII GTNAC 1 cut(s) 873
MboII GAAGA 4 cut(s) 16, 638, 896, 1220
MflI RGATCY 3 cut(s) 434, 773, 812
MhlI GDGCHC 1 cut(s) 577
MlsI TGGCCA 1 cut(s) 844
MluNI TGGCCA 1 cut(s) 844
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 2 cut(s) 108, 1285
MnlI CCTC 7 cut(s) 41, 119, 423, 661, 727, 864, 1039
Mox20I TGGCCA 1 cut(s) 844
MscI TGGCCA 1 cut(s) 844
MseI TTAA 3 cut(s) 294, 369, 380
MslI CAYNNNNRTG 2 cut(s) 485, 838
Msp20I TGGCCA 1 cut(s) 844
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 6 cut(s) 68, 428, 444, 528, 789, 1299
MvaI CCWGG 5 cut(s) 68, 428, 528, 789, 1299
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 5 cut(s) 24, 50, 187, 748, 991
NciI CCSGG 1 cut(s) 444
NcoI CCATGG 1 cut(s) 1099
NlaIII CATG 9 cut(s) 205, 339, 709, 723, 755, 822, 947, 1103, 1193
NlaIV GGNNCC 1 cut(s) 309
NmeAIII GCCGAG 1 cut(s) 635
NruI TCGCGA 1 cut(s) 83
NspI RCATGY 1 cut(s) 339
OliI CACNNNNGTG 1 cut(s) 838
PaeI GCATGC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 883, 1328
PfoI TCCNGGA 1 cut(s) 787
PkrI GCNGC 7 cut(s) 46, 49, 52, 319, 492, 673, 1314
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Ppu21I YACGTR 1 cut(s) 483
PsiI TTATAA 1 cut(s) 504
Psp6I CCWGG 5 cut(s) 66, 426, 526, 787, 1297
PspGI CCWGG 5 cut(s) 66, 426, 526, 787, 1297
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 3 cut(s) 250, 363, 1295
PsuI RGATCY 3 cut(s) 434, 773, 812
PvuII CAGCTG 1 cut(s) 47
RruI TCGCGA 1 cut(s) 83
RsaI GTAC 3 cut(s) 142, 1116, 1157
RsaNI GTAC 3 cut(s) 141, 1115, 1156
RseI CAYNNNNRTG 2 cut(s) 485, 838
SalI GTCGAC 1 cut(s) 894
SaqAI TTAA 3 cut(s) 294, 369, 380
SatI GCNGC 7 cut(s) 45, 48, 51, 318, 491, 672, 1313
Sau96I GGNCC 3 cut(s) 250, 363, 1295
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 6 cut(s) 68, 428, 444, 528, 789, 1299
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 2 cut(s) 106, 792
SinI GGWCC 2 cut(s) 363, 1295
SmiMI CAYNNNNRTG 2 cut(s) 485, 838
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
SphI GCATGC 1 cut(s) 339
SsiI CCGC 1 cut(s) 51
SspMI CTAG 3 cut(s) 224, 810, 951
StyD4I CCNGG 6 cut(s) 66, 426, 442, 526, 787, 1297
StyI CCWWGG 2 cut(s) 1099, 1229
TaaI ACNGT 4 cut(s) 96, 288, 963, 1155
TaiI ACGT 2 cut(s) 485, 1282
TaqI TCGA 2 cut(s) 624, 895
TatI WGTACW 3 cut(s) 140, 1114, 1155
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 2 cut(s) 883, 1328
Tru1I TTAA 3 cut(s) 294, 369, 380
Tru9I TTAA 3 cut(s) 294, 369, 380
TscAI CASTG 3 cut(s) 143, 318, 582
TseI GCWGC 6 cut(s) 44, 47, 317, 490, 671, 1312
TspDTI ATGAA 4 cut(s) 17, 46, 218, 486
TspRI CASTG 3 cut(s) 143, 318, 582
VpaK11BI GGWCC 2 cut(s) 363, 1295
XagI CCTNNNNNAGG 2 cut(s) 689, 1043
XapI RAATTY 3 cut(s) 7, 391, 1200
XceI RCATGY 1 cut(s) 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 852
XmiI GTMKAC 3 cut(s) 895, 1150, 1225
XspI CTAG 3 cut(s) 224, 810, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.