Rorug06G0327600

NADH dehydrogenase ubiquinone 1 beta subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016651
Physical Location & Seq
Forward (+)
48053237 .. 48054344
1108 bp
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UTR
Exon/CDS
Intron
Rorug06G0327600.1

Sequence Viewer

Length: 504 bp
ATGGCTTTGGCCAAGAGTTTAATGATATTTTTCTTGGTGATGGCTTGTTTCGGGGTTTGCTTTGGTGCCGTTTACAAGGTGGGTGACTCTGAGGGATGGAGATATAAAGGTTTCAACTACACGGCTTGGAGACTTGATAAAGACTTCACTGTTGGAGACTCTCTCTTGTTTGTGTACAAAGGAAAGGACAGTGTAGTGCAAGTCGATCGCAAACATTACCAAACATGCAATTCGACGGATCCAATTAACGAAACCAGATCTGGCAACGATACTATTACCCTAGACAAGCCTGGCCACTTCTTCTACATCACCGGTTACAAGGAACACTGCGAGCGTGGACAGAAGCTTGACATCAGAGTTTCCCCTCCAACTAGTCCAAGTCCAAGTCCAAGCCCTAGCCAATCACCACACAAGAGCAGTGCTCCATCCATCCAATCTTCAGCTGCGATACCTGCTCTAATATTGATGGCTATGGCTGTGGTCGTCCTTTCCTCTACTGCCTAA

Protein Analysis

167

Amino Acids

18.33

Weight (kDa)

8.66

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 114 1.4e-22 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016651)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76200
fragaria_vesca FvH4_2g37630
malus_domestica MD08G1003800.v1.1 MD15G1003400.v1.1
prunus_persica Prupe.1G355800_v2.0.a1 Prupe.1G355800_v2.0.a1
pyrus_communis pycom08g00290
rosa_chinensis RchiOBHm_Chr6g0304101
rosa_roxburghii Rroxscaffold_7G00164070
rosa_rugosa Rorug06G0327600
rosa_samantha Rh6BG461200 Rh6CG453800 Rh6DG441000
rosa_wichuraiana Rw6G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 460
AccB1I GGYRCC 1 cut(s) 65
AclWI GGATC 2 cut(s) 233, 246
AcoI YGGCCR 2 cut(s) 9, 292
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 1 cut(s) 176
AgeI ACCGGT 1 cut(s) 311
AgsI TTSAA 1 cut(s) 115
AhlI ACTAGT 1 cut(s) 371
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 2 cut(s) 346, 443
AluI AGCT 2 cut(s) 346, 443
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 2 cut(s) 124, 150
AlwI GGATC 2 cut(s) 233, 246
AoxI GGCC 2 cut(s) 9, 292
ApeKI GCWGC 1 cut(s) 443
AsiGI ACCGGT 1 cut(s) 311
AsuHPI GGTGA 4 cut(s) 49, 95, 301, 396
BalI TGGCCA 2 cut(s) 11, 294
BamHI GGATCC 1 cut(s) 238
BanI GGYRCC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 1 cut(s) 430
BccI CCATC 5 cut(s) 34, 90, 433, 437, 460
BceAI ACGGC 2 cut(s) 53, 138
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 124, 150
BcuI ACTAGT 1 cut(s) 371
BfaI CTAG 3 cut(s) 281, 372, 396
BfuAI ACCTGC 1 cut(s) 460
BglII AGATCT 1 cut(s) 257
BisI GCNGC 1 cut(s) 444
BlsI GCNGC 1 cut(s) 445
Bme1390I CCNGG 1 cut(s) 291
BmiI GGNNCC 2 cut(s) 67, 240
BmrFI CCNGG 1 cut(s) 291
BplI GAGNNNNNCTC 4 cut(s) 147, 179, 406, 438
BsaWI WCCGGW 1 cut(s) 311
Bse118I RCCGGY 1 cut(s) 311
BseBI CCWGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 101, 425, 429
BseMII CTCAG 1 cut(s) 81
BseXI GCAGC 1 cut(s) 430
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 2 cut(s) 11, 294
BshNI GGYRCC 1 cut(s) 65
BshTI ACCGGT 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 424
BsiSI CCGG 1 cut(s) 312
BsmAI GTCTC 2 cut(s) 124, 150
BsnI GGCC 2 cut(s) 11, 294
Bsp1286I GDGCHC 1 cut(s) 424
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 3 cut(s) 205, 238, 257
BspANI GGCC 2 cut(s) 11, 294
BspCNI CTCAG 1 cut(s) 82
BspLI GGNNCC 2 cut(s) 67, 240
BspMI ACCTGC 1 cut(s) 460
BspPI GGATC 2 cut(s) 233, 246
BspT107I GGYRCC 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 311
BssMI GATC 3 cut(s) 205, 238, 257
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 151, 191
BstAUI TGTACA 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 3 cut(s) 101, 425, 429
BstKTI GATC 3 cut(s) 208, 241, 260
BstMAI GTCTC 2 cut(s) 124, 150
BstMBI GATC 3 cut(s) 205, 238, 257
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 430
BstX2I RGATCY 2 cut(s) 238, 257
BstYI RGATCY 2 cut(s) 238, 257
BsuRI GGCC 2 cut(s) 11, 294
BtsCI GGATG 3 cut(s) 101, 425, 429
BtsI GCAGTG 2 cut(s) 325, 424
BtsIMutI CAGTG 4 cut(s) 147, 196, 325, 424
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 311
Csp6I GTAC 1 cut(s) 175
CspAI ACCGGT 1 cut(s) 311
CviAII CATG 1 cut(s) 225
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 90
DpnI GATC 3 cut(s) 207, 240, 259
DpnII GATC 3 cut(s) 205, 238, 257
EaeI YGGCCR 2 cut(s) 9, 292
Eco57I CTGAAG 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 289
FaeI CATG 1 cut(s) 228
FaiI YATR 3 cut(s) 105, 226, 473
FatI CATG 1 cut(s) 224
Fnu4HI GCNGC 1 cut(s) 444
FokI GGATG 3 cut(s) 108, 412, 416
Fsp4HI GCNGC 1 cut(s) 444
FspBI CTAG 3 cut(s) 281, 372, 396
GluI GCNGC 1 cut(s) 444
HaeIII GGCC 2 cut(s) 11, 294
HapII CCGG 1 cut(s) 312
Hin1II CATG 1 cut(s) 228
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 2 cut(s) 86, 158
HpaII CCGG 1 cut(s) 312
HphI GGTGA 4 cut(s) 49, 95, 301, 396
Hpy166II GTNNAC 3 cut(s) 73, 175, 338
Hpy188I TCNGA 2 cut(s) 91, 356
Hpy8I GTNNAC 3 cut(s) 73, 175, 338
Hpy99I CGWCG 1 cut(s) 238
HpyCH4III ACNGT 2 cut(s) 151, 191
HpyCH4V TGCA 2 cut(s) 199, 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 1 cut(s) 90
Hsp92II CATG 1 cut(s) 228
Kzo9I GATC 3 cut(s) 205, 238, 257
LmnI GCTCC 1 cut(s) 427
LpnPI CCDG 6 cut(s) 246, 268, 276, 303, 325, 465
Lsp1109I GCAGC 1 cut(s) 430
MaeI CTAG 3 cut(s) 281, 372, 396
MaeIII GTNAC 2 cut(s) 83, 314
MalI GATC 3 cut(s) 207, 240, 259
MboI GATC 3 cut(s) 205, 238, 257
MboII GAAGA 2 cut(s) 292, 429
MflI RGATCY 2 cut(s) 238, 257
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 2 cut(s) 11, 294
MluCI AATT 2 cut(s) 229, 243
MluNI TGGCCA 2 cut(s) 11, 294
MlyI GAGTC 2 cut(s) 80, 152
MmeI TCCRAC 2 cut(s) 133, 392
MnlI CCTC 3 cut(s) 85, 375, 502
Mox20I TGGCCA 2 cut(s) 11, 294
MscI TGGCCA 2 cut(s) 11, 294
MseI TTAA 2 cut(s) 20, 246
Msp20I TGGCCA 2 cut(s) 11, 294
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 452
NdeII GATC 3 cut(s) 205, 238, 257
NlaIII CATG 1 cut(s) 228
NlaIV GGNNCC 2 cut(s) 67, 240
NmuCI GTSAC 1 cut(s) 83
NspI RCATGY 1 cut(s) 228
PinAI ACCGGT 1 cut(s) 311
PkrI GCNGC 1 cut(s) 445
Ple19I CGATCG 1 cut(s) 208
PleI GAGTC 2 cut(s) 80, 152
PpsI GAGTC 2 cut(s) 80, 152
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 67, 240
PsuI RGATCY 2 cut(s) 238, 257
PvuI CGATCG 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SaqAI TTAA 2 cut(s) 20, 246
SatI GCNGC 1 cut(s) 444
Sau3AI GATC 3 cut(s) 205, 238, 257
SchI GAGTC 2 cut(s) 80, 152
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 424
SetI ASST 5 cut(s) 81, 112, 348, 445, 454
SpeI ACTAGT 1 cut(s) 371
Sse9I AATT 2 cut(s) 229, 243
SspI AATATT 1 cut(s) 462
SspMI CTAG 3 cut(s) 281, 372, 396
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 2 cut(s) 151, 191
TaqI TCGA 2 cut(s) 204, 233
TasI AATT 2 cut(s) 229, 243
TatI WGTACW 1 cut(s) 174
Tru1I TTAA 2 cut(s) 20, 246
Tru9I TTAA 2 cut(s) 20, 246
TscAI CASTG 4 cut(s) 154, 196, 332, 424
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 1 cut(s) 83
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 4 cut(s) 154, 196, 332, 424
XceI RCATGY 1 cut(s) 228
XspI CTAG 3 cut(s) 281, 372, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.