Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0009562

Physical Location & Seq

Genomic Coordinates Reverse (-)

49037823 .. 49046230

Gene Structure 8408 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0338600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3348 bp

ATGGAGGAACCAGCCACCGGCGGCCGCCCGAAGAGGACTAGGGCTCACACTCGAACCACCACTGAGAACAACGCTAGCGATGCGAGTGAACACGTGGACCGCGAGAGCTCGCCGGACGATTTCGAAGCGCCTCGTCCTAAAGCTAAACGAGGTCGCCCTCCCAGTGGCTCCACCGCCGCGCCGCCCAAAGCCTCCGCTCTCACTCTGATTGAGGTCGTCAAGGGCAACGGGAAACTTATTCCTCAGGCGGTCAAGCTCTGGGTTGAGCGGTACGAAAAGGATGCTAAACCTGCTACTGTTGAGCTCTTGACAATGTTGTTCGAGGCATGTGGAGCTAAGTATCATATTAAAGAAGAGTTGCTGGATGAGACTAATGTTGACGATGTCGTGGTTGCTCTTGTCGAGCTTGCTAGAAGTGGTAACGTGGATGATTACCAAAGTTCAAAAAAGGAGTTTAAAAACATGAAGGACAATCTCCAATCATTCTGGGATAAGTTGGTCTGCGAATGTCAACACGGGCCATTATTTGATCAGATTTTGTTCGAGAAGTGCATGGGCTATATAATTGCTTTGTCATGCACTCCTCCAAGAGTTTATCGCCAAACTGCTACTTTGATGGGTCTCCAACTTGTTACTTCATTCATAACTGTTGCTAAAACACTTGGTGTTCAGCGTGAAACTACTCGAAGACAATTAGAGGCTGAAAAAAAAAAACAAGCTGAAGGACCTCGTGTGGAGTCACTGAACAAGAGGTTCTCGATGACTCATGAAAACATAACTATATTAGAGCAGATGATGCGCAATATATTTCAAGGGTTATTTGTGCATCGCTACCGAGACATTGAACCAAATATTCGGACGTCGTGTATAGAGGCACTCGGCGTGTGGATTTTGTCATATCCATCAATGTTCTTGCAGGATTTGTACTTGAAGTATCTTGGATGGACATTGAATGACAAAAATGCTGGTGTAAGAAAAGCATCTGTGCTTGCATTGCAAAATCTTTATGAGGTTGATGATAATGTGCCAACTCTGGGCCTATTCACTGAAAGATTTTCTACTCGGATGATTGAGCTTGCTGATGACATTGATATTTCTGTGGCTGTATCTGCCATTGGCCTTGTTAAACAGCTTTTAAGACATCAGCTTCTACCAGATGATGACTTAGGTCCTTTATATGATTTGCTTATTGATGATCCAGCAGAGATCAGGCATGCTATAGGGGCATTAGTGTATGAACACTTGATTTCTCAGAAGTTCATTAGTTCCCAGTCTGGTGCAAAAGGTGTTGAGAACAATTCTTCTGAGGTCCAGCTTGGCAGAATGTTGCAAATTCTAAGAGAGTTCTCTGCAGATCCAATTCTTAGCGTTTATGTAATTGATGATGTTTGGGAGTACATGAATGCCATGAAGGATTGGAAGTGTATTATCTCAATGCTCTTGGATGAGAATCCACTGATTGAGCTTACTGATGAGGATGCAACAAACTTGGTACGGCTTCTTTCTGCATCTGTCAAAAAGGCTGTAGGAGAGAGGATAGTTCCTGCTACTGATAATCGAAAGCCATACTACACTAAAGCTCAAAAAGATGTATTTGAGCACAACAAACAGGATATAACTCTTGCCATGATGAAGAACTATCCACTACTTCTCCGTAAGTTCATGGCAGACAAAGCAAAAATACCATCACTGGTTGACATTATTCTACACATGAATCTTGGGCTATATTCCAAGAGGAGGCAGGAGCAGAATTTTGAAACCGTCCTTCAACTGATCAAAGAGGCATTTTTCAAACATGGTGAGAAGGAGGCGCTGAGATCTTGTGCAAATGCTATCATGCTTTGTTCAACAGACAGTCAAGGGGAGCTAAAAGATACGGCCCGTAATTTAGTAAAGGAACTTCATGATGAGCTTATTCATAATAAGCTTAAACCTGCGATGAAAGAAGTGGCGGATGGTGGTGATGAATATCCTCTTCTTGTAAATTTGAAGAGAATGTATGAGCTTCAATTGTCAGGAAATGTGCCTATCGAGAGTTTGTATGAAGATATGGTCTCAACTCTTCAGAGCTATACAAACAAGGATGATCAGGTTGTCACTTTTCTGCTTCTCAACATGTATATGCATGTGCAATGGTCTCTGCATGCCATCATACATAGTCCTACTATCTCAGAGGCATCTTTATCTTCTATATTAGCGAAACGTGATTCTTTGTTAGAGCGACTAGAGTACATCCTTAACAGCTGTCTTGAAATGGAGGGTAATCGCTGCAATCAGCTAGCTCGCTGGGTCTGTGTTATACTCACTGATTTGTCTAGACTGTTCAAAAAGACAAACTTTGCTTCGTCGAAGCTGGAAAAATTAGGATATTCTCCAGATGCATTGGTTCTTCAGAAGTTTTGGAAACTTTGTGTGCAGCAGCTCAGTATTTCAGATGAGACGGAAGATGACGATGGGAACAAAGAGTATATTGAGGAGACAAATAGAGATACTGTCATGATGGCTGCTGTACGGTTAGCTGTTACTGATAAAGTTTATAAGGAGTACCTGGGTCCAGAGATTATTTCTAGATTTGTGATGCATGGTACAAGTGTAGGGGAGATAGTTAAGCATCTGATAACTTTTCTGAAGAAGAATGATGATGATCTTCCAAAAACTTTCTTGGAAGCGCTGAAAATGGCCTACCATCGGTATATGGTTGGACTATCTAGAAGTGAAGATGAATCTTTAGCAAGCAAGCATTTTCTAGAGTTTCAAGAACTAGCTGCTCAGCTTTCTGGGACATTTATAGGTGCTTCCAAGAACAAGCACAAATCAGACATTTTAAAAATTGTTAACGAGGGAATTGAATATGCTTTTATAGACGCTCCAAAGCAGTTGTCCTTTCTGGAAGGTGCTGTGCTCCATTTTGTGTCCAAATTGCCTACACCTGATATTCTGGAGATAAGTAACGTCAATATTCAAAATCGAACGGAGAATGTAAATATGGATGAAGATCCCAGTGGCTGGCGTCCTTATCACACATTCGTTCAGACTTTGCGGGAAAAATTTGCAAAGATTGAAGGTATCCAAGAAGAAAAGGACGGGACCTCTGTAAGGCGCAGGGGTCGTCCACGGAAAAGGCGTAATATACAGGGAAAGAGGCTGTTTGATGATCATGGTTCAAGTGAAGAAGAGGATTCAATTAGTGGATCTGACCATGAAAATGCTCAAGATGAAGAAGAACAACAAGATGAGGATGAAGATGATGATGACGATGAAGACGTTCCTTTGATAAATCAAATTAGGTCATCATCCAAGTTGAGATCGCTGAAAGTTTCACGAGAGGGAAACAAAGGCCAGACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005338 GO:0005459 GO:0005460 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0006811 GO:0006820 GO:0007275 GO:0008150 GO:0008509 GO:0008514 GO:0009653 GO:0009791 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0015075 GO:0015165 GO:0015711 GO:0015780 GO:0015786 GO:0015931 GO:0015932 GO:0021700 GO:0022622 GO:0022857 GO:0030154 GO:0032501 GO:0032502 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0055085 GO:0071695 GO:0071702 GO:0071705 GO:0072334 GO:0080147 GO:0090481 GO:0090558 GO:0090627 GO:0090696 GO:0098656 GO:0099402 GO:1901264 GO:1901505 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPT

Protein Analysis

1115

Amino Acids

126.86

Weight (kDa)

5.47

Isoelectric Point (pI)

50.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
STAG	PF08514	146 - 250	2.6e-27	STAG domain
SCD	PF21581	279 - 352	1.5e-29	Stromalin conservative domain
HEAT_SCC3-SA	PF24571	458 - 712	1.4e-70	Cohesin subunit SCC3/SA, HEAT-repeats domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009562)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31600 AT4G31600
fragaria_vesca	FvH4_2g36601 FvH4_2g36601 FvH4_2g36601
malus_domestica	MD08G1126100.v1.1 MD15G1105900.v1.1
prunus_persica	Prupe.1G457700_v2.0.a1
pyrus_communis	pycom08g10530 pycom15g09610
rosa_chinensis	RchiOBHm_Chr6g0305211
rosa_laevigata	RLG00000010908
rosa_multiflora	Rmu_sc0000061.1_g000005
rosa_roxburghii	Rroxscaffold_7G00163040
rosa_rugosa	Rorug06G0338600 Rorug06G0338600 Rorug06G0338600
rosa_samantha	Rh6AG450300 Rh6BG451100 Rh6CG464100 Rh6DG450900
rosa_wichuraiana	Rw6G039240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2538
AatII	GACGTC	1 cut(s)	863
Acc16I	TGCGCA	1 cut(s)	800
Acc36I	ACCTGC	2 cut(s)	298, 1942
AccB7I	CCANNNNNTGG	1 cut(s)	3006
AccBSI	CCGCTC	2 cut(s)	197, 268
AccII	CGCG	2 cut(s)	102, 179
AclWI	GGATC	4 cut(s)	1190, 1349, 2990, 3199
AcoI	YGGCCR	1 cut(s)	22
AcsI	RAATTY	4 cut(s)	1332, 1750, 1984, 3047
AcuI	CTGAAG	4 cut(s)	741, 2048, 2375, 2648
AcvI	CACGTG	1 cut(s)	94
AcyI	GRCGYC	2 cut(s)	860, 3010
AdeI	CACNNNGTG	1 cut(s)	665
AfaI	GTAC	8 cut(s)	272, 926, 1397, 1494, 2231, 2511, 2546, 2587
AfeI	AGCGCT	1 cut(s)	2670
AfiI	CCNNNNNNNGG	7 cut(s)	17, 164, 1034, 1737, 2688, 3006, 3096
AflIII	ACRYGT	2 cut(s)	91, 2115
AjnI	CCWGG	1 cut(s)	2547
Alw21I	GWGCWC	4 cut(s)	110, 306, 1602, 2904
Alw26I	GTCTC	7 cut(s)	362, 626, 831, 2059, 2142, 2432, 2471
AlwI	GGATC	4 cut(s)	1190, 1349, 2990, 3199
AlwNI	CAGNNNCTG	1 cut(s)	3006
Aor51HI	AGCGCT	1 cut(s)	2670
AoxI	GGCC	7 cut(s)	22, 518, 1036, 1117, 1878, 2679, 3337
ApeKI	GCWGC	5 cut(s)	2268, 2416, 2419, 2504, 2765
ApoI	RAATTY	4 cut(s)	1332, 1750, 1984, 3047
ArsI	GACNNNNNNTTYG	2 cut(s)	2864, 2896
Asp700I	GAANNNNTTC	1 cut(s)	3264
AspLEI	GCGC	6 cut(s)	130, 181, 801, 1813, 2671, 3102
AspS9I	GGNCC	9 cut(s)	97, 518, 725, 1036, 1169, 1309, 1879, 2552, 3087
AsuHPI	GGTGA	2 cut(s)	1811, 1973
AsuII	TTCGAA	1 cut(s)	123
AsuNHI	GCTAGC	2 cut(s)	74, 2278
AvaII	GGWCC	6 cut(s)	97, 725, 1169, 1309, 2552, 3087
AxyI	CCTNAGG	1 cut(s)	243
BaeI	ACNNNNGTAYC	2 cut(s)	2577, 2610
BanII	GRGCYC	3 cut(s)	46, 110, 306
BarI	GAAGNNNNNNTAC	2 cut(s)	1884, 1916
BauI	CACGAG	2 cut(s)	729, 3321
BbrPI	CACGTG	1 cut(s)	94
BbsI	GAAGAC	2 cut(s)	694, 3267
Bbv12I	GWGCWC	4 cut(s)	110, 306, 1602, 2904
BbvI	GCAGC	5 cut(s)	2255, 2428, 2431, 2491, 2752
BccI	CCATC	9 cut(s)	610, 910, 936, 1693, 1949, 2156, 2447, 2494, 2694
BceAI	ACGGC	2 cut(s)	1511, 1893
BcgI	CGANNNNNNTGC	2 cut(s)	263, 297
BciT130I	CCWGG	1 cut(s)	2549
BciVI	GTATCC	1 cut(s)	3077
BclI	TGATCA	4 cut(s)	529, 1773, 2086, 3154
BcoDI	GTCTC	7 cut(s)	362, 626, 831, 2059, 2142, 2432, 2471
BfmI	CTRYAG	3 cut(s)	1218, 1350, 1524
BfoI	RGCGCY	3 cut(s)	131, 1814, 2672
BfuAI	ACCTGC	2 cut(s)	298, 1942
BfuI	GTATCC	1 cut(s)	3077
BglII	AGATCT	1 cut(s)	1817
BisI	GCNGC	9 cut(s)	22, 25, 177, 182, 2269, 2417, 2420, 2505, 2766
BlpI	GCTNAGC	1 cut(s)	2769
BlsI	GCNGC	9 cut(s)	23, 26, 178, 183, 2270, 2418, 2421, 2506, 2767
Bme1390I	CCNGG	1 cut(s)	2549
Bme18I	GGWCC	6 cut(s)	97, 725, 1169, 1309, 2552, 3087
BmgT120I	GGNCC	9 cut(s)	97, 518, 725, 1036, 1169, 1309, 1879, 2552, 3087
BmiI	GGNNCC	4 cut(s)	9, 169, 2553, 3088
BmrFI	CCNGG	1 cut(s)	2549
BmrI	ACTGGG	3 cut(s)	156, 1264, 2994
BmtI	GCTAGC	2 cut(s)	78, 2282
BmuI	ACTGGG	3 cut(s)	156, 1264, 2994
BoxI	GACNNNNGTC	1 cut(s)	1167
BpiI	GAAGAC	2 cut(s)	694, 3267
BpmI	CTGGAG	2 cut(s)	2358, 2960
Bpu1102I	GCTNAGC	1 cut(s)	2769
Bpu14I	TTCGAA	1 cut(s)	123
BpuEI	CTTGAG	1 cut(s)	3195
BsaAI	YACGTR	1 cut(s)	94
BsaBI	GATNNNNATC	4 cut(s)	1449, 1968, 2643, 2994
BsaHI	GRCGYC	2 cut(s)	860, 3010
BsaI	GGTCTC	3 cut(s)	626, 2059, 2142
BsaJI	CCNNGG	2 cut(s)	2548, 3113
BsaXI	ACNNNNNCTCC	2 cut(s)	1635, 1665
Bsc4I	CCNNNNNNNGG	7 cut(s)	17, 164, 1034, 1737, 2688, 3006, 3096
Bse118I	RCCGGY	1 cut(s)	17
Bse1I	ACTGG	4 cut(s)	162, 1270, 1695, 3000
Bse21I	CCTNAGG	1 cut(s)	243
Bse3DI	GCAATG	2 cut(s)	992, 2138
Bse8I	GATNNNNATC	4 cut(s)	1449, 1968, 2643, 2994
BseBI	CCWGG	1 cut(s)	2549
BseDI	CCNNGG	2 cut(s)	2548, 3113
BseJI	GATNNNNATC	4 cut(s)	1449, 1968, 2643, 2994
BseLI	CCNNNNNNNGG	7 cut(s)	17, 164, 1034, 1737, 2688, 3006, 3096
BseMI	GCAATG	2 cut(s)	992, 2138
BseMII	CTCAG	8 cut(s)	54, 257, 1265, 1296, 1805, 2184, 2437, 2783
BseNI	ACTGG	4 cut(s)	162, 1270, 1695, 3000
BseRI	GAGGAG	3 cut(s)	573, 1750, 2489
BseX3I	CGGCCG	1 cut(s)	22
BseXI	GCAGC	5 cut(s)	2255, 2428, 2431, 2491, 2752
BseYI	CCCAGC	1 cut(s)	2286
BsgI	GTGCAG	1 cut(s)	2435
Bsh1236I	CGCG	2 cut(s)	102, 179
Bsh1285I	CGRYCG	1 cut(s)	25
BshFI	GGCC	7 cut(s)	24, 520, 1038, 1119, 1880, 2681, 3339
BsiEI	CGRYCG	1 cut(s)	25
BsiHKAI	GWGCWC	4 cut(s)	110, 306, 1602, 2904
BsiSI	CCGG	2 cut(s)	18, 113
BslFI	GGGAC	2 cut(s)	2794, 3100
BslI	CCNNNNNNNGG	7 cut(s)	17, 164, 1034, 1737, 2688, 3006, 3096
BsmAI	GTCTC	7 cut(s)	362, 626, 831, 2059, 2142, 2432, 2471
BsmBI	CGTCTC	1 cut(s)	2432
BsmFI	GGGAC	2 cut(s)	2794, 3100
BsmI	GAATGC	1 cut(s)	1408
BsnI	GGCC	7 cut(s)	24, 520, 1038, 1119, 1880, 2681, 3339
Bso31I	GGTCTC	3 cut(s)	626, 2059, 2142
Bsp119I	TTCGAA	1 cut(s)	123
Bsp1286I	GDGCHC	5 cut(s)	46, 110, 306, 1602, 2904
Bsp1720I	GCTNAGC	1 cut(s)	2769
BspANI	GGCC	7 cut(s)	24, 520, 1038, 1119, 1880, 2681, 3339
BspCNI	CTCAG	8 cut(s)	55, 256, 1264, 1297, 1806, 2183, 2436, 2782
BspFNI	CGCG	2 cut(s)	102, 179
BspHI	TCATGA	3 cut(s)	766, 1903, 2496
BspLI	GGNNCC	4 cut(s)	9, 169, 2553, 3088
BspMAI	CTGCAG	1 cut(s)	1354
BspMI	ACCTGC	2 cut(s)	298, 1942
BspOI	GCTAGC	2 cut(s)	78, 2282
BspPI	GGATC	4 cut(s)	1190, 1349, 2990, 3199
BspT104I	TTCGAA	1 cut(s)	123
BspTNI	GGTCTC	3 cut(s)	626, 2059, 2142
BsrBI	CCGCTC	2 cut(s)	197, 268
BsrDI	GCAATG	2 cut(s)	992, 2138
BsrFI	RCCGGY	1 cut(s)	17
BsrI	ACTGG	4 cut(s)	162, 1270, 1695, 3000
BssAI	RCCGGY	1 cut(s)	17
BssECI	CCNNGG	2 cut(s)	2548, 3113
BssNI	GRCGYC	2 cut(s)	860, 3010
BssSI	CACGAG	2 cut(s)	729, 3321
Bst2BI	CACGAG	2 cut(s)	729, 3321
Bst2UI	CCWGG	1 cut(s)	2549
Bst4CI	ACNGT	7 cut(s)	298, 649, 1762, 1856, 2322, 2494, 2514
Bst6I	CTCTTC	6 cut(s)	26, 348, 1980, 1985, 2067, 3168
BstACI	GRCGYC	2 cut(s)	860, 3010
BstAPI	GCANNNNNTGC	1 cut(s)	796
BstBAI	YACGTR	1 cut(s)	94
BstBI	TTCGAA	1 cut(s)	123
BstDSI	CCRYGG	1 cut(s)	3113
BstENI	CCTNNNNNAGG	1 cut(s)	3094
BstFNI	CGCG	2 cut(s)	102, 179
BstH2I	RGCGCY	3 cut(s)	131, 1814, 2672
BstHHI	GCGC	6 cut(s)	130, 181, 801, 1813, 2671, 3102
BstMAI	GTCTC	7 cut(s)	362, 626, 831, 2059, 2142, 2432, 2471
BstMCI	CGRYCG	1 cut(s)	25
BstMWI	GCNNNNNNNGC	8 cut(s)	80, 290, 332, 796, 994, 1109, 1223, 1673
BstNI	CCWGG	1 cut(s)	2549
BstNSI	RCATGY	5 cut(s)	330, 1217, 2119, 2129, 2147
BstPAI	GACNNNNGTC	1 cut(s)	1167
BstSCI	CCNGG	1 cut(s)	2547
BstSFI	CTRYAG	3 cut(s)	1218, 1350, 1524
BstUI	CGCG	2 cut(s)	102, 179
BstV1I	GCAGC	5 cut(s)	2255, 2428, 2431, 2491, 2752
BstV2I	GAAGAC	2 cut(s)	694, 3267
BstX2I	RGATCY	4 cut(s)	1354, 1817, 2995, 3191
BstYI	RGATCY	4 cut(s)	1354, 1817, 2995, 3191
BstZI	CGGCCG	1 cut(s)	22
Bsu36I	CCTNAGG	1 cut(s)	243
BsuI	GTATCC	1 cut(s)	3077
BsuRI	GGCC	7 cut(s)	24, 520, 1038, 1119, 1880, 2681, 3339
BtgI	CCRYGG	1 cut(s)	3113
BtgZI	GCGATG	3 cut(s)	93, 812, 1952
BtsIMutI	CAGTG	8 cut(s)	60, 169, 740, 1044, 1454, 1688, 2304, 3007
BveI	ACCTGC	2 cut(s)	298, 1942
CaiI	CAGNNNCTG	1 cut(s)	3006
CciI	TCATGA	3 cut(s)	766, 1903, 2496
CciNI	GCGGCCGC	1 cut(s)	22
CfoI	GCGC	6 cut(s)	130, 181, 801, 1813, 2671, 3102
Cfr10I	RCCGGY	1 cut(s)	17
Cfr13I	GGNCC	9 cut(s)	97, 518, 725, 1036, 1169, 1309, 1879, 2552, 3087
CseI	GACGC	2 cut(s)	2873, 2999
Csp6I	GTAC	8 cut(s)	271, 925, 1396, 1493, 2230, 2510, 2545, 2586
CviQI	GTAC	8 cut(s)	271, 925, 1396, 1493, 2230, 2510, 2545, 2586
DraI	TTTAAA	2 cut(s)	457, 2826
DraIII	CACNNNGTG	1 cut(s)	665
EaeI	YGGCCR	1 cut(s)	22
EagI	CGGCCG	1 cut(s)	22
Eam1104I	CTCTTC	6 cut(s)	26, 348, 1980, 1985, 2067, 3168
EarI	CTCTTC	6 cut(s)	26, 348, 1980, 1985, 2067, 3168
EciI	GGCGGA	1 cut(s)	1967
Ecl136II	GAGCTC	2 cut(s)	108, 304
EclXI	CGGCCG	1 cut(s)	22
Eco24I	GRGCYC	3 cut(s)	46, 110, 306
Eco31I	GGTCTC	3 cut(s)	626, 2059, 2142
Eco47I	GGWCC	6 cut(s)	97, 725, 1169, 1309, 2552, 3087
Eco47III	AGCGCT	1 cut(s)	2670
Eco52I	CGGCCG	1 cut(s)	22
Eco53kI	GAGCTC	2 cut(s)	108, 304
Eco57I	CTGAAG	4 cut(s)	741, 2048, 2375, 2648
Eco72I	CACGTG	1 cut(s)	94
Eco81I	CCTNAGG	1 cut(s)	243
EcoICRI	GAGCTC	2 cut(s)	108, 304
EcoNI	CCTNNNNNAGG	1 cut(s)	3094
EcoO109I	RGGNCCY	3 cut(s)	725, 1169, 3087
EcoRII	CCWGG	1 cut(s)	2547
EcoT22I	ATGCAT	3 cut(s)	2127, 2383, 2583
EcoT38I	GRGCYC	3 cut(s)	46, 110, 306
Esp3I	CGTCTC	1 cut(s)	2432
FalI	AAGNNNNNCTT	2 cut(s)	2321, 2353
FaqI	GGGAC	2 cut(s)	2794, 3100
FauI	CCCGC	1 cut(s)	3033
FbaI	TGATCA	4 cut(s)	529, 1773, 2086, 3154
Fnu4HI	GCNGC	9 cut(s)	22, 25, 177, 182, 2269, 2417, 2420, 2505, 2766
FriOI	GRGCYC	3 cut(s)	46, 110, 306
Fsp4HI	GCNGC	9 cut(s)	22, 25, 177, 182, 2269, 2417, 2420, 2505, 2766
FspI	TGCGCA	1 cut(s)	800
GlaI	GCGC	6 cut(s)	129, 180, 800, 1812, 2670, 3101
GluI	GCNGC	9 cut(s)	22, 25, 177, 182, 2269, 2417, 2420, 2505, 2766
GsaI	CCCAGC	1 cut(s)	2290
GsuI	CTGGAG	2 cut(s)	2358, 2960
HaeII	RGCGCY	3 cut(s)	131, 1814, 2672
HaeIII	GGCC	7 cut(s)	24, 520, 1038, 1119, 1880, 2681, 3339
HapII	CCGG	2 cut(s)	18, 113
HgaI	GACGC	2 cut(s)	2873, 2999
HhaI	GCGC	6 cut(s)	130, 181, 801, 1813, 2671, 3102
Hin1I	GRCGYC	2 cut(s)	860, 3010
Hin6I	GCGC	6 cut(s)	128, 179, 799, 1811, 2669, 3100
HinP1I	GCGC	6 cut(s)	128, 179, 799, 1811, 2669, 3100
HincII	GTYRAC	4 cut(s)	379, 512, 1696, 2836
HindII	GTYRAC	4 cut(s)	379, 512, 1696, 2836
HindIII	AAGCTT	1 cut(s)	1925
HinfI	GANTC	7 cut(s)	737, 763, 1450, 1714, 2207, 2723, 3179
HpaI	GTTAAC	1 cut(s)	2836
HpaII	CCGG	2 cut(s)	18, 113
HphI	GGTGA	2 cut(s)	1811, 1973
Hpy166II	GTNNAC	7 cut(s)	89, 97, 379, 512, 1696, 2836, 3113
Hpy8I	GTNNAC	7 cut(s)	89, 97, 379, 512, 1696, 2836, 3113
Hpy99I	CGWCG	2 cut(s)	865, 2350
HpyAV	CCTTC	7 cut(s)	460, 716, 1405, 1775, 1798, 2885, 3056
HpyCH4III	ACNGT	7 cut(s)	298, 649, 1762, 1856, 2322, 2494, 2514
HpyCH4IV	ACGT	7 cut(s)	93, 423, 860, 2203, 2952, 3264, 3344
HpyF10VI	GCNNNNNNNGC	8 cut(s)	80, 290, 332, 796, 994, 1109, 1223, 1673
HpySE526I	ACGT	7 cut(s)	93, 423, 860, 2203, 2952, 3264, 3344
Hsp92I	GRCGYC	2 cut(s)	860, 3010
HspAI	GCGC	6 cut(s)	128, 179, 799, 1811, 2669, 3100
Ksp22I	TGATCA	4 cut(s)	529, 1773, 2086, 3154
KspAI	GTTAAC	1 cut(s)	2836
LmnI	GCTCC	6 cut(s)	173, 332, 1744, 1864, 2872, 2907
Lsp1109I	GCAGC	5 cut(s)	2255, 2428, 2431, 2491, 2752
MaeII	ACGT	7 cut(s)	93, 423, 860, 2203, 2952, 3264, 3344
MaeIII	GTNAC	6 cut(s)	419, 631, 738, 2095, 2521, 2948
MbiI	CCGCTC	2 cut(s)	197, 268
MfeI	CAATTG	1 cut(s)	2009
MflI	RGATCY	4 cut(s)	1354, 1817, 2995, 3191
MhlI	GDGCHC	5 cut(s)	46, 110, 306, 1602, 2904
MlyI	GAGTC	2 cut(s)	746, 757
MmeI	TCCRAC	2 cut(s)	649, 2680
Mph1103I	ATGCAT	3 cut(s)	2127, 2383, 2583
MroXI	GAANNNNTTC	1 cut(s)	3264
MseI	TTAA	9 cut(s)	348, 456, 1125, 1136, 1929, 2238, 2607, 2825, 2835
MslI	CAYNNNNRTG	2 cut(s)	2120, 3204
MspA1I	CMGCKG	1 cut(s)	2244
MspI	CCGG	2 cut(s)	18, 113
MspR9I	CCNGG	1 cut(s)	2549
MunI	CAATTG	1 cut(s)	2009
Mva1269I	GAATGC	1 cut(s)	1408
MvaI	CCWGG	1 cut(s)	2549
MvnI	CGCG	2 cut(s)	102, 179
MwoI	GCNNNNNNNGC	8 cut(s)	80, 290, 332, 796, 994, 1109, 1223, 1673
NheI	GCTAGC	2 cut(s)	74, 2278
NlaIV	GGNNCC	4 cut(s)	9, 169, 2553, 3088
NmeAIII	GCCGAG	1 cut(s)	858
NmuCI	GTSAC	2 cut(s)	738, 2095
NotI	GCGGCCGC	1 cut(s)	22
NsbI	TGCGCA	1 cut(s)	800
NsiI	ATGCAT	3 cut(s)	2127, 2383, 2583
NspI	RCATGY	5 cut(s)	330, 1217, 2119, 2129, 2147
NspV	TTCGAA	1 cut(s)	123
PaeI	GCATGC	2 cut(s)	1217, 2147
PagI	TCATGA	3 cut(s)	766, 1903, 2496
PciI	ACATGT	1 cut(s)	2115
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 3121, 3261
PctI	GAATGC	1 cut(s)	1408
PdmI	GAANNNNTTC	1 cut(s)	3264
PfeI	GAWTC	5 cut(s)	1450, 1714, 2207, 2723, 3179
PflFI	GACNNNGTC	1 cut(s)	383
PflMI	CCANNNNNTGG	1 cut(s)	3006
PkrI	GCNGC	9 cut(s)	23, 26, 178, 183, 2270, 2418, 2421, 2506, 2767
PleI	GAGTC	2 cut(s)	745, 757
PmaCI	CACGTG	1 cut(s)	94
PmlI	CACGTG	1 cut(s)	94
PpsI	GAGTC	2 cut(s)	745, 757
Ppu21I	YACGTR	1 cut(s)	94
PpuMI	RGGWCCY	3 cut(s)	725, 1169, 3087
PscI	ACATGT	1 cut(s)	2115
PshAI	GACNNNNGTC	1 cut(s)	1167
PsiI	TTATAA	1 cut(s)	2538
Psp124BI	GAGCTC	2 cut(s)	110, 306
Psp5II	RGGWCCY	3 cut(s)	725, 1169, 3087
Psp6I	CCWGG	1 cut(s)	2547
PspCI	CACGTG	1 cut(s)	94
PspFI	CCCAGC	1 cut(s)	2286
PspGI	CCWGG	1 cut(s)	2547
PspN4I	GGNNCC	4 cut(s)	9, 169, 2553, 3088
PspPI	GGNCC	9 cut(s)	97, 518, 725, 1036, 1169, 1309, 1879, 2552, 3087
PspPPI	RGGWCCY	3 cut(s)	725, 1169, 3087
PstI	CTGCAG	1 cut(s)	1354
PstNI	CAGNNNCTG	1 cut(s)	3006
PsuI	RGATCY	4 cut(s)	1354, 1817, 2995, 3191
PsyI	GACNNNGTC	1 cut(s)	383
PvuII	CAGCTG	1 cut(s)	2244
RsaI	GTAC	8 cut(s)	272, 926, 1397, 1494, 2231, 2511, 2546, 2587
RsaNI	GTAC	8 cut(s)	271, 925, 1396, 1493, 2230, 2510, 2545, 2586
RseI	CAYNNNNRTG	2 cut(s)	2120, 3204
SacI	GAGCTC	2 cut(s)	110, 306
SaqAI	TTAA	9 cut(s)	348, 456, 1125, 1136, 1929, 2238, 2607, 2825, 2835
SatI	GCNGC	9 cut(s)	22, 25, 177, 182, 2269, 2417, 2420, 2505, 2766
Sau96I	GGNCC	9 cut(s)	97, 518, 725, 1036, 1169, 1309, 1879, 2552, 3087
SchI	GAGTC	2 cut(s)	746, 757
ScrFI	CCNGG	1 cut(s)	2549
SduI	GDGCHC	5 cut(s)	46, 110, 306, 1602, 2904
SfcI	CTRYAG	3 cut(s)	1218, 1350, 1524
SfuI	TTCGAA	1 cut(s)	123
SgrAI	CRCCGGYG	1 cut(s)	17
SinI	GGWCC	6 cut(s)	97, 725, 1169, 1309, 2552, 3087
SmiMI	CAYNNNNRTG	2 cut(s)	2120, 3204
SmlI	CTYRAG	1 cut(s)	3210
SmoI	CTYRAG	1 cut(s)	3210
SphI	GCATGC	2 cut(s)	1217, 2147
SspI	AATATT	2 cut(s)	853, 2959
SstI	GAGCTC	2 cut(s)	110, 306
StyD4I	CCNGG	1 cut(s)	2547
TaaI	ACNGT	7 cut(s)	298, 649, 1762, 1856, 2322, 2494, 2514
TaiI	ACGT	7 cut(s)	96, 426, 863, 2206, 2955, 3267, 3347
TatI	WGTACW	3 cut(s)	924, 1395, 2229
TauI	GCSGC	4 cut(s)	24, 27, 179, 184
TfiI	GAWTC	5 cut(s)	1450, 1714, 2207, 2723, 3179
Tru1I	TTAA	9 cut(s)	348, 456, 1125, 1136, 1929, 2238, 2607, 2825, 2835
Tru9I	TTAA	9 cut(s)	348, 456, 1125, 1136, 1929, 2238, 2607, 2825, 2835
TscAI	CASTG	8 cut(s)	67, 169, 747, 1051, 1461, 1695, 2311, 3007
TseFI	GTSAC	2 cut(s)	738, 2095
TseI	GCWGC	5 cut(s)	2268, 2416, 2419, 2504, 2765
Tsp45I	GTSAC	2 cut(s)	738, 2095
TspGWI	ACGGA	4 cut(s)	1643, 2456, 2987, 3130
TspRI	CASTG	8 cut(s)	67, 169, 747, 1051, 1461, 1695, 2311, 3007
Tth111I	GACNNNGTC	1 cut(s)	383
Van91I	CCANNNNNTGG	1 cut(s)	3006
VpaK11BI	GGWCC	6 cut(s)	97, 725, 1169, 1309, 2552, 3087
XagI	CCTNNNNNAGG	1 cut(s)	3094
XapI	RAATTY	4 cut(s)	1332, 1750, 1984, 3047
XbaI	TCTAGA	4 cut(s)	2315, 2567, 2708, 2746
XceI	RCATGY	5 cut(s)	330, 1217, 2119, 2129, 2147
XmnI	GAANNNNTTC	1 cut(s)	3264
ZraI	GACGTC	1 cut(s)	861
Zsp2I	ATGCAT	3 cut(s)	2127, 2383, 2583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.