Rorug06G0435000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010902
Physical Location & Seq
Reverse (-)
56987626 .. 57000961
13336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0435000.1

Sequence Viewer

Length: 474 bp
ATGACCACGAATCTTCAGAAATGGAGAGTCGCACTGAACCTCCTCCGCCGCTACGACGGCGTTTCGGCTCACAACGCCCGGCGTCTATTGCACGACGGACCTGACACCGTGGAGGAGCTGCTAGACAGACACGTGGTGAAGAAAGAGAAACACCTGGACGACGAAGAGGAGGAGCTGCTGAATCGGCGGCGACTGACGACCACGCGCCGGGAGGCGCTGTGTCTTTATCGAGACATTCTCAGGGCGACGCGGTTCTTCATGTGGACGGACTCTCGGGGCGTGCTTTGGCGAGATGTGCTGAGAGAGAACGCGAGGAAGGAGTTCGAGGAGGCCCGGTTCGAAACGGATCCGGAAGTTGTGACCCGATTACTGATCGGAGGGCACGATGCTGTGCAGTCGGCGTTAGATAAGCTCGCCGAGAAGCAGCGGCAGCAGATTCAGAAGGAGCGAGGCCGCGGTGGAGATCAACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

18.63

Weight (kDa)

8.82

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_LYR PF05347 70 - 131 8.8e-10 Complex 1 protein (LYR family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 205, 250, 311, 456
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 7 cut(s) 46, 49, 187, 250, 427, 454, 456
AclWI GGATC 2 cut(s) 341, 354
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 1 cut(s) 82
AdeI CACNNNGTG 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 130
AjnI CCWGG 1 cut(s) 153
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
AluBI AGCT 3 cut(s) 118, 175, 412
AluI AGCT 3 cut(s) 118, 175, 412
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 2 cut(s) 341, 354
Ama87I CYCGRG 1 cut(s) 273
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 2 cut(s) 330, 451
ApeKI GCWGC 4 cut(s) 118, 175, 424, 430
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 2 cut(s) 207, 217
AspS9I GGNCC 2 cut(s) 98, 331
AsuC2I CCSGG 3 cut(s) 79, 209, 334
AsuHPI GGTGA 1 cut(s) 148
AsuII TTCGAA 1 cut(s) 339
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 384
BamHI GGATCC 1 cut(s) 346
BbrPI CACGTG 1 cut(s) 133
BbvI GCAGC 4 cut(s) 105, 162, 436, 442
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 155
BcnI CCSGG 3 cut(s) 79, 209, 334
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 122
BfoI RGCGCY 1 cut(s) 218
BisI GCNGC 8 cut(s) 49, 119, 176, 188, 425, 428, 431, 454
BlsI GCNGC 8 cut(s) 50, 120, 177, 189, 426, 429, 432, 455
Bme1390I CCNGG 4 cut(s) 79, 155, 209, 334
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 98, 331
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 4 cut(s) 79, 155, 209, 334
BmsI GCATC 1 cut(s) 376
BplI GAGNNNNNCTC 2 cut(s) 222, 254
Bpu14I TTCGAA 1 cut(s) 339
BpuMI CCSGG 3 cut(s) 79, 209, 334
BsaAI YACGTR 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 108, 454
BsaWI WCCGGW 1 cut(s) 349
BsaXI ACNNNNNCTCC 6 cut(s) 24, 54, 203, 233, 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 207
BseAI TCCGGA 1 cut(s) 349
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 2 cut(s) 108, 454
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 253, 290
BseRI GAGGAG 5 cut(s) 32, 128, 182, 185, 341
BseSI GKGCMC 1 cut(s) 384
BseXI GCAGC 4 cut(s) 105, 162, 436, 442
BsgI GTGCAG 1 cut(s) 413
Bsh1236I CGCG 4 cut(s) 205, 250, 311, 456
BshFI GGCC 2 cut(s) 332, 453
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 4 cut(s) 79, 208, 334, 350
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 225
BsnI GGCC 2 cut(s) 332, 453
BsoBI CYCGRG 1 cut(s) 273
Bsp119I TTCGAA 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 384
Bsp13I TCCGGA 1 cut(s) 349
Bsp143I GATC 3 cut(s) 346, 372, 463
BspACI CCGC 7 cut(s) 46, 49, 187, 250, 427, 454, 456
BspANI GGCC 2 cut(s) 332, 453
BspCNI CTCAG 2 cut(s) 252, 291
BspEI TCCGGA 1 cut(s) 349
BspFNI CGCG 4 cut(s) 205, 250, 311, 456
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 2 cut(s) 341, 354
BspT104I TTCGAA 1 cut(s) 339
BssECI CCNNGG 2 cut(s) 108, 454
BssMI GATC 3 cut(s) 346, 372, 463
BssNI GRCGYC 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 2 cut(s) 109, 471
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 82
BstBAI YACGTR 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 281, 414
BstDEI CTNAG 2 cut(s) 239, 299
BstDSI CCRYGG 2 cut(s) 108, 454
BstFNI CGCG 4 cut(s) 205, 250, 311, 456
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 2 cut(s) 207, 217
BstKTI GATC 3 cut(s) 349, 375, 466
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 3 cut(s) 346, 372, 463
BstMWI GCNNNNNNNGC 6 cut(s) 57, 74, 88, 184, 295, 430
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 4 cut(s) 77, 153, 207, 332
BstSLI GKGCMC 1 cut(s) 384
BstUI CGCG 4 cut(s) 205, 250, 311, 456
BstV1I GCAGC 4 cut(s) 105, 162, 436, 442
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BsuRI GGCC 2 cut(s) 332, 453
BtgI CCRYGG 2 cut(s) 108, 454
BtsIMutI CAGTG 1 cut(s) 32
Cac8I GCNNGC 2 cut(s) 281, 414
CfoI GCGC 2 cut(s) 207, 217
Cfr13I GGNCC 2 cut(s) 98, 331
Cfr42I CCGCGG 1 cut(s) 457
CseI GACGC 2 cut(s) 71, 256
CviAII CATG 1 cut(s) 259
CviJI RGCY 6 cut(s) 68, 118, 175, 332, 412, 453
CviKI_1 RGCY 6 cut(s) 68, 118, 175, 332, 412, 453
DdeI CTNAG 2 cut(s) 239, 299
DpnI GATC 3 cut(s) 348, 374, 465
DpnII GATC 3 cut(s) 346, 372, 463
DraIII CACNNNGTG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 98
Eco72I CACGTG 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 153
FaeI CATG 1 cut(s) 262
FaiI YATR 1 cut(s) 260
FatI CATG 1 cut(s) 258
Fnu4HI GCNGC 8 cut(s) 49, 119, 176, 188, 425, 428, 431, 454
Fsp4HI GCNGC 8 cut(s) 49, 119, 176, 188, 425, 428, 431, 454
FspBI CTAG 1 cut(s) 122
GlaI GCGC 2 cut(s) 206, 216
GluI GCNGC 8 cut(s) 49, 119, 176, 188, 425, 428, 431, 454
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 2 cut(s) 332, 453
HapII CCGG 4 cut(s) 79, 208, 334, 350
HgaI GACGC 2 cut(s) 71, 256
HhaI GCGC 2 cut(s) 207, 217
Hin1I GRCGYC 1 cut(s) 82
Hin1II CATG 1 cut(s) 262
Hin6I GCGC 2 cut(s) 205, 215
HinP1I GCGC 2 cut(s) 205, 215
HinfI GANTC 5 cut(s) 10, 27, 181, 269, 436
HpaII CCGG 4 cut(s) 79, 208, 334, 350
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 3 cut(s) 18, 377, 441
Hpy188III TCNNGA 2 cut(s) 230, 350
Hpy8I GTNNAC 1 cut(s) 264
Hpy99I CGWCG 4 cut(s) 59, 98, 164, 250
HpyAV CCTTC 2 cut(s) 310, 436
HpyCH4III ACNGT 2 cut(s) 109, 471
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 2 cut(s) 91, 394
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 74, 88, 184, 295, 430
HpyF3I CTNAG 2 cut(s) 239, 299
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 82
Hsp92II CATG 1 cut(s) 262
HspAI GCGC 2 cut(s) 205, 215
Kpn2I TCCGGA 1 cut(s) 349
KspI CCGCGG 1 cut(s) 457
Kzo9I GATC 3 cut(s) 346, 372, 463
LmnI GCTCC 3 cut(s) 115, 172, 445
LpnPI CCDG 8 cut(s) 92, 114, 140, 167, 221, 226, 347, 363
Lsp1109I GCAGC 4 cut(s) 105, 162, 436, 442
LweI GCATC 1 cut(s) 376
MaeI CTAG 1 cut(s) 122
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 358
MalI GATC 3 cut(s) 348, 374, 465
MboI GATC 3 cut(s) 346, 372, 463
MboII GAAGA 4 cut(s) 5, 151, 176, 247
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 384
MlyI GAGTC 2 cut(s) 36, 263
MroI TCCGGA 1 cut(s) 349
MroXI GAANNNNTTC 1 cut(s) 320
MspA1I CMGCKG 2 cut(s) 427, 456
MspI CCGG 4 cut(s) 79, 208, 334, 350
MspR9I CCNGG 4 cut(s) 79, 155, 209, 334
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 4 cut(s) 205, 250, 311, 456
MwoI GCNNNNNNNGC 6 cut(s) 57, 74, 88, 184, 295, 430
NciI CCSGG 3 cut(s) 79, 209, 334
NdeII GATC 3 cut(s) 346, 372, 463
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 1 cut(s) 348
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 358
NspV TTCGAA 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 3 cut(s) 10, 181, 436
PkrI GCNGC 8 cut(s) 50, 120, 177, 189, 426, 429, 432, 455
PleI GAGTC 2 cut(s) 35, 263
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 2 cut(s) 35, 263
Ppu21I YACGTR 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 153
PspCI CACGTG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 2 cut(s) 98, 331
PsuI RGATCY 1 cut(s) 346
SacII CCGCGG 1 cut(s) 457
SatI GCNGC 8 cut(s) 49, 119, 176, 188, 425, 428, 431, 454
Sau3AI GATC 3 cut(s) 346, 372, 463
Sau96I GGNCC 2 cut(s) 98, 331
SchI GAGTC 2 cut(s) 36, 263
ScrFI CCNGG 4 cut(s) 79, 155, 209, 334
SduI GDGCHC 1 cut(s) 384
SetI ASST 7 cut(s) 42, 103, 120, 135, 156, 177, 414
SfaNI GCATC 1 cut(s) 376
Sfr303I CCGCGG 1 cut(s) 457
SfuI TTCGAA 1 cut(s) 339
SgrBI CCGCGG 1 cut(s) 457
SinI GGWCC 1 cut(s) 98
SsiI CCGC 7 cut(s) 46, 49, 187, 250, 427, 454, 456
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 4 cut(s) 77, 153, 207, 332
TaaI ACNGT 2 cut(s) 109, 471
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 229, 324, 339
TauI GCSGC 4 cut(s) 51, 190, 430, 456
TfiI GAWTC 3 cut(s) 10, 181, 436
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 4 cut(s) 118, 175, 424, 430
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 3 cut(s) 111, 281, 359
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.