Rorug06G0468300

lysM domain-containing GPI-anchored protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010568
Physical Location & Seq
Forward (+)
59241203 .. 59243855
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0468300.1

Sequence Viewer

Length: 978 bp
ATGACTCCACATTCAATCTTAAACTCTTTTGAAGAAACACTCAGTTGGGCTTACAGTGAGCCCAAATTGAAGGTTTGGATCAGTAGCCCAACCCAATCCAAAAGCCCATACTCTATTTCACCTGAACTGCCATTGTCGAAAAGTGTCACCTTCACATTCCCAGTGCTACCTCCTCTCTCCGCTTTTCCCCGTCGATTGTTTTTTGGTGGCAAAACCGTCAACCAAATAATGGAGAAGATGATAGCTCTCAGATCGCTCTGCCGTTCACTCTCTTCCAGATCTTACCGAACAGCCGCCGTCGCCGCCGCCACCCAGCAGCTGCCACGTCACCAACCTCACCACCACTCCGCCGGCGCTGCTCGAACCCTCTTCAGTTTCGCTTCCCCAAACTCCAAGAACCAGTCTTCCGTATCATGTACAGATGAACACAGGTCTCCTCTTTCAACCAGTTTGGGGAGTAGGCGGTTTTCCAGTAACGATGTGGGTCATTTTCCTGAGATTAAAGACCCTGTGCTGCTTAATGCTTTTAAGGACTTGATGGCTGCAAGTTGGGATGAGCTCCCTAAGCTTGTCGTCCGTGAAGTTAAGGCAGCATTGTCTAAGAACACTGATGATCAGACTGGCAAAGAGCATCTCATGAATGTGTTGCGTGCGGCCGAAGCTGTGGAGGAGTTTGGAGGGATGGTTATGCAGTTGAAGATGGATATGGATGACAGTGTCGGTCTGAGTGGAGAGGATGTGAAGCCGTTGTCAGATGAGTATGTGAATGCTCTGAAGACGATTTACGGTCGCTACGCTGCCTATTTGGATTCATTTGGGCCTGACGAGACCTATTTGAGGAAGAAGGTGGAGACTGAGCTGGGAACCAGGCTGATATACTTGAAGATGAGGTGTAGTGGCATTGGTTCTGAGTGGGGAAAGGTCAGTGTTCTTGGAACTTCTGGACTGTCAGGGTCATATGTTGAGCAAAGAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM

Protein Analysis

325

Amino Acids

35.92

Weight (kDa)

8.81

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDH5_plant PF14290 77 - 325 4.4e-106 Succinate dehydrogenase subunit 5, mitochondrial
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 229
AciI CCGC 7 cut(s) 180, 294, 303, 306, 348, 463, 653
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 654
AcuI CTGAAG 2 cut(s) 355, 794
AfaI GTAC 1 cut(s) 418
AfiI CCNNNNNNNGG 3 cut(s) 229, 453, 837
AgsI TTSAA 6 cut(s) 15, 32, 70, 444, 697, 883
AjiI CACGTC 1 cut(s) 326
AjnI CCWGG 1 cut(s) 866
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
AluBI AGCT 7 cut(s) 245, 319, 559, 568, 662, 859, 974
AluI AGCT 7 cut(s) 245, 319, 559, 568, 662, 859, 974
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 3 cut(s) 438, 821, 845
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 1 cut(s) 319
AoxI GGCC 2 cut(s) 654, 818
ApeKI GCWGC 7 cut(s) 316, 319, 356, 514, 542, 590, 797
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 818
AsuHPI GGTGA 4 cut(s) 111, 139, 320, 329
BanII GRGCYC 2 cut(s) 63, 561
BarI GAAGNNNNNNTAC 2 cut(s) 767, 799
BbsI GAAGAC 2 cut(s) 396, 782
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 7 cut(s) 306, 328, 343, 501, 529, 602, 784
BccI CCATC 3 cut(s) 532, 676, 694
BceAI ACGGC 3 cut(s) 246, 281, 730
BciT130I CCWGG 1 cut(s) 868
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 3 cut(s) 438, 821, 845
BfoI RGCGCY 1 cut(s) 357
BglII AGATCT 1 cut(s) 278
Bme1390I CCNGG 1 cut(s) 868
BmgBI CACGTC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 1 cut(s) 865
BmrFI CCNGG 1 cut(s) 868
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 1 cut(s) 640
BmuI ACTGGG 1 cut(s) 155
BpiI GAAGAC 2 cut(s) 396, 782
Bpu10I CCTNAGC 1 cut(s) 564
BsaI GGTCTC 2 cut(s) 438, 821
BsaXI ACNNNNNCTCC 6 cut(s) 329, 359, 669, 699, 723, 753
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 453, 837
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 5 cut(s) 161, 400, 447, 471, 625
BseBI CCWGG 1 cut(s) 868
BseGI GGATG 4 cut(s) 559, 687, 715, 742
BseLI CCNNNNNNNGG 3 cut(s) 229, 453, 837
BseMII CTCAG 6 cut(s) 55, 262, 486, 716, 846, 900
BseNI ACTGG 5 cut(s) 161, 400, 447, 471, 625
BseRI GAGGAG 3 cut(s) 162, 426, 683
BseX3I CGGCCG 1 cut(s) 654
BseXI GCAGC 7 cut(s) 306, 328, 343, 501, 529, 602, 784
BseYI CCCAGC 2 cut(s) 312, 859
Bsh1285I CGRYCG 2 cut(s) 657, 790
BshFI GGCC 2 cut(s) 656, 820
BsiEI CGRYCG 2 cut(s) 657, 790
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 1 cut(s) 351
BslI CCNNNNNNNGG 3 cut(s) 229, 453, 837
BsmAI GTCTC 3 cut(s) 438, 821, 845
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 2 cut(s) 656, 820
Bso31I GGTCTC 2 cut(s) 438, 821
Bsp1286I GDGCHC 2 cut(s) 63, 561
Bsp1407I TGTACA 1 cut(s) 416
Bsp143I GATC 4 cut(s) 78, 251, 278, 613
BspACI CCGC 7 cut(s) 180, 294, 303, 306, 348, 463, 653
BspANI GGCC 2 cut(s) 656, 820
BspCNI CTCAG 6 cut(s) 54, 261, 487, 717, 847, 901
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 1 cut(s) 865
BspPI GGATC 1 cut(s) 86
BspTNI GGTCTC 2 cut(s) 438, 821
BsrFI RCCGGY 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 416
BsrI ACTGG 5 cut(s) 161, 400, 447, 471, 625
BssAI RCCGGY 1 cut(s) 350
BssMI GATC 4 cut(s) 78, 251, 278, 613
Bst2UI CCWGG 1 cut(s) 868
Bst4CI ACNGT 5 cut(s) 56, 217, 716, 788, 948
Bst6I CTCTTC 2 cut(s) 277, 374
BstAUI TGTACA 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 352, 651
BstDEI CTNAG 9 cut(s) 41, 248, 495, 564, 600, 725, 855, 909, 975
BstENI CCTNNNNNAGG 1 cut(s) 835
BstF5I GGATG 4 cut(s) 559, 687, 715, 742
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 4 cut(s) 81, 254, 281, 616
BstMAI GTCTC 3 cut(s) 438, 821, 845
BstMBI GATC 4 cut(s) 78, 251, 278, 613
BstMCI CGRYCG 2 cut(s) 657, 790
BstMWI GCNNNNNNNGC 5 cut(s) 299, 302, 356, 565, 659
BstNI CCWGG 1 cut(s) 868
BstSCI CCNGG 1 cut(s) 866
BstV1I GCAGC 7 cut(s) 306, 328, 343, 501, 529, 602, 784
BstV2I GAAGAC 2 cut(s) 396, 782
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 654
BsuRI GGCC 2 cut(s) 656, 820
BtrI CACGTC 1 cut(s) 326
BtsCI GGATG 4 cut(s) 559, 687, 715, 742
BtsIMutI CAGTG 5 cut(s) 61, 168, 606, 721, 931
Cac8I GCNNGC 2 cut(s) 352, 651
CaiI CAGNNNCTG 1 cut(s) 319
CciI TCATGA 1 cut(s) 636
CfoI GCGC 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 818
Csp6I GTAC 1 cut(s) 417
CviAII CATG 2 cut(s) 414, 637
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 9 cut(s) 41, 248, 495, 564, 600, 725, 855, 909, 975
DpnI GATC 4 cut(s) 80, 253, 280, 615
DpnII GATC 4 cut(s) 78, 251, 278, 613
EaeI YGGCCR 1 cut(s) 654
EagI CGGCCG 1 cut(s) 654
Eam1104I CTCTTC 2 cut(s) 277, 374
EarI CTCTTC 2 cut(s) 277, 374
EciI GGCGGA 1 cut(s) 337
Ecl136II GAGCTC 1 cut(s) 559
EclXI CGGCCG 1 cut(s) 654
Eco24I GRGCYC 2 cut(s) 63, 561
Eco31I GGTCTC 2 cut(s) 438, 821
Eco52I CGGCCG 1 cut(s) 654
Eco53kI GAGCTC 1 cut(s) 559
Eco57I CTGAAG 2 cut(s) 355, 794
EcoICRI GAGCTC 1 cut(s) 559
EcoNI CCTNNNNNAGG 1 cut(s) 835
EcoRII CCWGG 1 cut(s) 866
EcoT38I GRGCYC 2 cut(s) 63, 561
FaeI CATG 2 cut(s) 417, 640
FaiI YATR 9 cut(s) 109, 415, 638, 689, 707, 762, 877, 958, 960
FatI CATG 2 cut(s) 413, 636
FauNDI CATATG 1 cut(s) 958
FbaI TGATCA 1 cut(s) 613
FokI GGATG 4 cut(s) 566, 694, 722, 749
FriOI GRGCYC 2 cut(s) 63, 561
GlaI GCGC 1 cut(s) 355
GsaI CCCAGC 2 cut(s) 316, 863
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 2 cut(s) 656, 820
HapII CCGG 1 cut(s) 351
HhaI GCGC 1 cut(s) 356
Hin1II CATG 2 cut(s) 417, 640
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 2 cut(s) 4, 809
HpaII CCGG 1 cut(s) 351
HphI GGTGA 4 cut(s) 111, 139, 320, 329
Hpy166II GTNNAC 2 cut(s) 220, 266
Hpy188I TCNGA 6 cut(s) 251, 618, 726, 754, 774, 910
Hpy188III TCNNGA 4 cut(s) 276, 494, 637, 942
Hpy8I GTNNAC 2 cut(s) 220, 266
Hpy99I CGWCG 2 cut(s) 195, 302
HpyAV CCTTC 3 cut(s) 64, 160, 838
HpyCH4III ACNGT 5 cut(s) 56, 217, 716, 788, 948
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 2 cut(s) 545, 691
HpyF10VI GCNNNNNNNGC 5 cut(s) 299, 302, 356, 565, 659
HpyF3I CTNAG 9 cut(s) 41, 248, 495, 564, 600, 725, 855, 909, 975
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 2 cut(s) 417, 640
HspAI GCGC 1 cut(s) 354
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 4 cut(s) 78, 251, 278, 613
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 7 cut(s) 306, 328, 343, 501, 529, 602, 784
LweI GCATC 1 cut(s) 640
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 3 cut(s) 145, 326, 473
MalI GATC 4 cut(s) 80, 253, 280, 615
MboI GATC 4 cut(s) 78, 251, 278, 613
MboII GAAGA 9 cut(s) 44, 247, 264, 361, 396, 709, 787, 853, 895
MflI RGATCY 1 cut(s) 278
MhlI GDGCHC 2 cut(s) 63, 561
MluCI AATT 1 cut(s) 65
MreI CGCCGGCG 1 cut(s) 350
MroNI GCCGGC 1 cut(s) 350
MseI TTAA 5 cut(s) 20, 501, 519, 528, 585
MslI CAYNNNNRTG 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 1 cut(s) 868
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 1 cut(s) 868
MwoI GCNNNNNNNGC 5 cut(s) 299, 302, 356, 565, 659
NaeI GCCGGC 1 cut(s) 352
NdeI CATATG 1 cut(s) 958
NdeII GATC 4 cut(s) 78, 251, 278, 613
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 2 cut(s) 417, 640
NlaIV GGNNCC 1 cut(s) 865
NmuCI GTSAC 2 cut(s) 145, 326
PagI TCATGA 1 cut(s) 636
PctI GAATGC 1 cut(s) 772
PdiI GCCGGC 1 cut(s) 352
PfeI GAWTC 1 cut(s) 809
PflFI GACNNNGTC 1 cut(s) 716
PflMI CCANNNNNTGG 1 cut(s) 229
Psp124BI GAGCTC 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 866
PspFI CCCAGC 2 cut(s) 312, 859
PspGI CCWGG 1 cut(s) 866
PspN4I GGNNCC 1 cut(s) 865
PspPI GGNCC 1 cut(s) 818
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 278
PsyI GACNNNGTC 1 cut(s) 716
PvuII CAGCTG 1 cut(s) 319
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
RseI CAYNNNNRTG 1 cut(s) 641
SacI GAGCTC 1 cut(s) 561
SaqAI TTAA 5 cut(s) 20, 501, 519, 528, 585
Sau3AI GATC 4 cut(s) 78, 251, 278, 613
Sau96I GGNCC 1 cut(s) 818
ScrFI CCNGG 1 cut(s) 868
SduI GDGCHC 2 cut(s) 63, 561
SfaNI GCATC 1 cut(s) 640
SgrAI CRCCGGYG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 641
Sse9I AATT 1 cut(s) 65
SsiI CCGC 7 cut(s) 180, 294, 303, 306, 348, 463, 653
SstI GAGCTC 1 cut(s) 561
StyD4I CCNGG 1 cut(s) 866
TaaI ACNGT 5 cut(s) 56, 217, 716, 788, 948
TaiI ACGT 1 cut(s) 328
TaqI TCGA 3 cut(s) 137, 193, 361
TaqII GACCGA 1 cut(s) 710
TasI AATT 1 cut(s) 65
TatI WGTACW 1 cut(s) 416
TauI GCSGC 4 cut(s) 296, 305, 308, 656
TfiI GAWTC 1 cut(s) 809
Tru1I TTAA 5 cut(s) 20, 501, 519, 528, 585
Tru9I TTAA 5 cut(s) 20, 501, 519, 528, 585
TscAI CASTG 5 cut(s) 61, 168, 613, 721, 931
TseFI GTSAC 2 cut(s) 145, 326
TseI GCWGC 7 cut(s) 316, 319, 356, 514, 542, 590, 797
Tsp45I GTSAC 2 cut(s) 145, 326
TspDTI ATGAA 3 cut(s) 438, 653, 801
TspGWI ACGGA 2 cut(s) 397, 566
TspRI CASTG 5 cut(s) 61, 168, 613, 721, 931
Tth111I GACNNNGTC 1 cut(s) 716
Van91I CCANNNNNTGG 1 cut(s) 229
XagI CCTNNNNNAGG 1 cut(s) 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.