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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g04040 FvH4_5g04040 FvH4_5g04040 |
| rosa_chinensis | RchiOBHm_Chr7g0195481 |
| rosa_laevigata | RLG00000004117 |
| rosa_multiflora | Rmu_sc0013420.1_g000004 Rmu_sc0019107.1_g000002 |
| rosa_roxburghii | Rroxscaffold_3G00260440 |
| rosa_rugosa | Rorug07G0023700 Rorug07G0023700 Rorug07G0023800 |
| rosa_samantha | Rh7AG151100 Rh7BG152800 Rh7CG156300 Rh7DG152100 |
| rosa_wichuraiana | Rw7G012900 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 2122, 4364 |
| Acc36I | ACCTGC | 3 cut(s) | 1466, 2122, 4364 |
| Acc65I | GGTACC | 1 cut(s) | 2030 |
| AccB1I | GGYRCC | 4 cut(s) | 1456, 2030, 3802, 4697 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 4172 |
| AccBSI | CCGCTC | 1 cut(s) | 676 |
| AccI | GTMKAC | 3 cut(s) | 810, 2840, 4260 |
| AccII | CGCG | 3 cut(s) | 368, 2139, 3781 |
| AccIII | TCCGGA | 1 cut(s) | 1745 |
| AciI | CCGC | 7 cut(s) | 339, 676, 1147, 1908, 2328, 3294, 4695 |
| AclWI | GGATC | 4 cut(s) | 619, 2098, 2990, 3837 |
| AcoI | YGGCCR | 5 cut(s) | 60, 556, 883, 2325, 3584 |
| AcsI | RAATTY | 4 cut(s) | 303, 923, 3105, 3368 |
| AcyI | GRCGYC | 1 cut(s) | 3670 |
| AdeI | CACNNNGTG | 1 cut(s) | 1901 |
| AfaI | GTAC | 3 cut(s) | 1993, 2032, 3699 |
| AflIII | ACRYGT | 3 cut(s) | 42, 1210, 3193 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 688 |
| AjiI | CACGTC | 1 cut(s) | 4606 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 175, 207, 249, 281, 2158, 2190 |
| AleI | CACNNNNGTG | 1 cut(s) | 3087 |
| Alw21I | GWGCWC | 7 cut(s) | 145, 1355, 1661, 1753, 2381, 2917, 3096 |
| Alw26I | GTCTC | 5 cut(s) | 675, 1587, 2515, 2974, 4596 |
| Alw44I | GTGCAC | 1 cut(s) | 2913 |
| AlwI | GGATC | 4 cut(s) | 619, 2098, 2990, 3837 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 2390, 3398, 4577 |
| Ama87I | CYCGRG | 3 cut(s) | 522, 2451, 3716 |
| Aor13HI | TCCGGA | 1 cut(s) | 1745 |
| ApaI | GGGCCC | 2 cut(s) | 2552, 3507 |
| ApaLI | GTGCAC | 1 cut(s) | 2913 |
| ApoI | RAATTY | 4 cut(s) | 303, 923, 3105, 3368 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 4207, 4239 |
| AseI | ATTAAT | 2 cut(s) | 480, 1859 |
| Asp718I | GGTACC | 1 cut(s) | 2030 |
| AspA2I | CCTAGG | 1 cut(s) | 1706 |
| AspLEI | GCGC | 2 cut(s) | 2139, 3781 |
| AsuC2I | CCSGG | 2 cut(s) | 1504, 3256 |
| AsuHPI | GGTGA | 8 cut(s) | 29, 146, 1022, 1455, 2103, 2421, 3172, 4605 |
| AvaI | CYCGRG | 3 cut(s) | 522, 2451, 3716 |
| AvaII | GGWCC | 9 cut(s) | 581, 991, 2443, 3201, 3935, 3986, 4208, 4223, 4448 |
| AvrII | CCTAGG | 1 cut(s) | 1706 |
| AxyI | CCTNAGG | 1 cut(s) | 4719 |
| BaeGI | GKGCMC | 4 cut(s) | 1078, 2552, 2917, 3507 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2370, 2403 |
| BanI | GGYRCC | 4 cut(s) | 1456, 2030, 3802, 4697 |
| BanII | GRGCYC | 6 cut(s) | 1355, 1661, 1753, 2552, 3062, 3507 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 4450, 4482 |
| Bbv12I | GWGCWC | 7 cut(s) | 145, 1355, 1661, 1753, 2381, 2917, 3096 |
| BbvCI | CCTCAGC | 1 cut(s) | 4662 |
| BceAI | ACGGC | 4 cut(s) | 75, 1444, 1718, 3056 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1641, 1675, 3989, 4023 |
| BclI | TGATCA | 4 cut(s) | 466, 1612, 1978, 3784 |
| BcnI | CCSGG | 2 cut(s) | 1504, 3256 |
| BcoDI | GTCTC | 5 cut(s) | 675, 1587, 2515, 2974, 4596 |
| BfmI | CTRYAG | 6 cut(s) | 2154, 3051, 3276, 3492, 3651, 4261 |
| BfuAI | ACCTGC | 3 cut(s) | 1466, 2122, 4364 |
| BglII | AGATCT | 1 cut(s) | 1924 |
| BlnI | CCTAGG | 1 cut(s) | 1706 |
| BlpI | GCTNAGC | 2 cut(s) | 291, 3591 |
| Bme18I | GGWCC | 9 cut(s) | 581, 991, 2443, 3201, 3935, 3986, 4208, 4223, 4448 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 688 |
| BmeT110I | CYCGRG | 3 cut(s) | 522, 2451, 3716 |
| BmgBI | CACGTC | 1 cut(s) | 4606 |
| BmrI | ACTGGG | 2 cut(s) | 2378, 4432 |
| BmuI | ACTGGG | 2 cut(s) | 2378, 4432 |
| BpmI | CTGGAG | 3 cut(s) | 1422, 2685, 4107 |
| Bpu10I | CCTNAGC | 4 cut(s) | 953, 2214, 3817, 4662 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 291, 3591 |
| BpuEI | CTTGAG | 3 cut(s) | 636, 2459, 3717 |
| BpuMI | CCSGG | 2 cut(s) | 1504, 3256 |
| Bsa29I | ATCGAT | 1 cut(s) | 3791 |
| BsaBI | GATNNNNATC | 3 cut(s) | 2016, 2490, 3840 |
| BsaHI | GRCGYC | 1 cut(s) | 3670 |
| BsaI | GGTCTC | 2 cut(s) | 675, 2974 |
| BsaWI | WCCGGW | 1 cut(s) | 1745 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 792, 822, 2350, 2380, 4210, 4240 |
| Bse118I | RCCGGY | 3 cut(s) | 420, 885, 4015 |
| Bse21I | CCTNAGG | 1 cut(s) | 4719 |
| Bse3DI | GCAATG | 2 cut(s) | 1784, 2416 |
| Bse8I | GATNNNNATC | 3 cut(s) | 2016, 2490, 3840 |
| BseAI | TCCGGA | 1 cut(s) | 1745 |
| BseCI | ATCGAT | 1 cut(s) | 3791 |
| BseJI | GATNNNNATC | 3 cut(s) | 2016, 2490, 3840 |
| BseMI | GCAATG | 2 cut(s) | 1784, 2416 |
| BseRI | GAGGAG | 5 cut(s) | 35, 813, 1064, 2389, 4679 |
| BseSI | GKGCMC | 4 cut(s) | 1078, 2552, 2917, 3507 |
| BseX3I | CGGCCG | 1 cut(s) | 60 |
| BsgI | GTGCAG | 2 cut(s) | 566, 968 |
| Bsh1236I | CGCG | 3 cut(s) | 368, 2139, 3781 |
| Bsh1285I | CGRYCG | 1 cut(s) | 63 |
| BshNI | GGYRCC | 4 cut(s) | 1456, 2030, 3802, 4697 |
| BshVI | ATCGAT | 1 cut(s) | 3791 |
| BsiEI | CGRYCG | 1 cut(s) | 63 |
| BsiHKAI | GWGCWC | 7 cut(s) | 145, 1355, 1661, 1753, 2381, 2917, 3096 |
| BsiHKCI | CYCGRG | 3 cut(s) | 522, 2451, 3716 |
| BsiSI | CCGG | 7 cut(s) | 421, 886, 1504, 1746, 3255, 3583, 4016 |
| BslFI | GGGAC | 4 cut(s) | 38, 1250, 2147, 2539 |
| BsmAI | GTCTC | 5 cut(s) | 675, 1587, 2515, 2974, 4596 |
| BsmBI | CGTCTC | 1 cut(s) | 4596 |
| BsmFI | GGGAC | 4 cut(s) | 38, 1250, 2147, 2539 |
| BsmI | GAATGC | 5 cut(s) | 1318, 1963, 2854, 3266, 4419 |
| Bso31I | GGTCTC | 2 cut(s) | 675, 2974 |
| BsoBI | CYCGRG | 3 cut(s) | 522, 2451, 3716 |
| Bsp120I | GGGCCC | 2 cut(s) | 2548, 3503 |
| Bsp13I | TCCGGA | 1 cut(s) | 1745 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 291, 3591 |
| Bsp19I | CCATGG | 1 cut(s) | 4625 |
| BspACI | CCGC | 7 cut(s) | 339, 676, 1147, 1908, 2328, 3294, 4695 |
| BspDI | ATCGAT | 1 cut(s) | 3791 |
| BspEI | TCCGGA | 1 cut(s) | 1745 |
| BspFNI | CGCG | 3 cut(s) | 368, 2139, 3781 |
| BspMAI | CTGCAG | 4 cut(s) | 2158, 3055, 3280, 3496 |
| BspMI | ACCTGC | 3 cut(s) | 1466, 2122, 4364 |
| BspPI | GGATC | 4 cut(s) | 619, 2098, 2990, 3837 |
| BspQI | GCTCTTC | 1 cut(s) | 2127 |
| BspT107I | GGYRCC | 4 cut(s) | 1456, 2030, 3802, 4697 |
| BspTNI | GGTCTC | 2 cut(s) | 675, 2974 |
| BsrBI | CCGCTC | 1 cut(s) | 676 |
| BsrDI | GCAATG | 2 cut(s) | 1784, 2416 |
| BsrFI | RCCGGY | 3 cut(s) | 420, 885, 4015 |
| BssAI | RCCGGY | 3 cut(s) | 420, 885, 4015 |
| BssNI | GRCGYC | 1 cut(s) | 3670 |
| BssT1I | CCWWGG | 7 cut(s) | 1283, 1402, 1706, 2244, 4478, 4625, 4748 |
| Bst6I | CTCTTC | 3 cut(s) | 2127, 2439, 2661 |
| BstACI | GRCGYC | 1 cut(s) | 3670 |
| BstDSI | CCRYGG | 1 cut(s) | 4625 |
| BstEII | GGTNACC | 2 cut(s) | 1287, 2109 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 4705, 4722 |
| BstFNI | CGCG | 3 cut(s) | 368, 2139, 3781 |
| BstHHI | GCGC | 2 cut(s) | 2139, 3781 |
| BstMAI | GTCTC | 5 cut(s) | 675, 1587, 2515, 2974, 4596 |
| BstMCI | CGRYCG | 1 cut(s) | 63 |
| BstNSI | RCATGY | 3 cut(s) | 46, 2243, 3197 |
| BstPI | GGTNACC | 2 cut(s) | 1287, 2109 |
| BstSFI | CTRYAG | 6 cut(s) | 2154, 3051, 3276, 3492, 3651, 4261 |
| BstSLI | GKGCMC | 4 cut(s) | 1078, 2552, 2917, 3507 |
| BstUI | CGCG | 3 cut(s) | 368, 2139, 3781 |
| BstX2I | RGATCY | 3 cut(s) | 1924, 2090, 3829 |
| BstYI | RGATCY | 3 cut(s) | 1924, 2090, 3829 |
| BstZI | CGGCCG | 1 cut(s) | 60 |
| Bsu15I | ATCGAT | 1 cut(s) | 3791 |
| Bsu36I | CCTNAGG | 1 cut(s) | 4719 |
| BsuTUI | ATCGAT | 1 cut(s) | 3791 |
| BtgI | CCRYGG | 1 cut(s) | 4625 |
| BtrI | CACGTC | 1 cut(s) | 4606 |
| BtsI | GCAGTG | 2 cut(s) | 1024, 4299 |
| BveI | ACCTGC | 3 cut(s) | 1466, 2122, 4364 |
| CaiI | CAGNNNCTG | 3 cut(s) | 2390, 3398, 4577 |
| CfoI | GCGC | 2 cut(s) | 2139, 3781 |
| Cfr10I | RCCGGY | 3 cut(s) | 420, 885, 4015 |
| ClaI | ATCGAT | 1 cut(s) | 3791 |
| CseI | GACGC | 2 cut(s) | 126, 3678 |
| CsiI | ACCWGGT | 2 cut(s) | 2203, 4450 |
| Csp6I | GTAC | 3 cut(s) | 1992, 2031, 3698 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1321, 1356 |
| CviQI | GTAC | 3 cut(s) | 1992, 2031, 3698 |
| DraI | TTTAAA | 3 cut(s) | 768, 1254, 2074 |
| DraIII | CACNNNGTG | 1 cut(s) | 1901 |
| DriI | GACNNNNNGTC | 1 cut(s) | 688 |
| EaeI | YGGCCR | 5 cut(s) | 60, 556, 883, 2325, 3584 |
| EagI | CGGCCG | 1 cut(s) | 60 |
| Eam1104I | CTCTTC | 3 cut(s) | 2127, 2439, 2661 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 688 |
| EarI | CTCTTC | 3 cut(s) | 2127, 2439, 2661 |
| Ecl136II | GAGCTC | 3 cut(s) | 1353, 1659, 1751 |
| EclXI | CGGCCG | 1 cut(s) | 60 |
| Eco130I | CCWWGG | 7 cut(s) | 1283, 1402, 1706, 2244, 4478, 4625, 4748 |
| Eco24I | GRGCYC | 6 cut(s) | 1355, 1661, 1753, 2552, 3062, 3507 |
| Eco31I | GGTCTC | 2 cut(s) | 675, 2974 |
| Eco47I | GGWCC | 9 cut(s) | 581, 991, 2443, 3201, 3935, 3986, 4208, 4223, 4448 |
| Eco52I | CGGCCG | 1 cut(s) | 60 |
| Eco53kI | GAGCTC | 3 cut(s) | 1353, 1659, 1751 |
| Eco81I | CCTNAGG | 1 cut(s) | 4719 |
| Eco88I | CYCGRG | 3 cut(s) | 522, 2451, 3716 |
| Eco91I | GGTNACC | 2 cut(s) | 1287, 2109 |
| EcoICRI | GAGCTC | 3 cut(s) | 1353, 1659, 1751 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 4705, 4722 |
| EcoO109I | RGGNCCY | 8 cut(s) | 848, 2443, 2548, 2549, 3503, 3504, 4448, 4574 |
| EcoO65I | GGTNACC | 2 cut(s) | 1287, 2109 |
| EcoT14I | CCWWGG | 7 cut(s) | 1283, 1402, 1706, 2244, 4478, 4625, 4748 |
| EcoT22I | ATGCAT | 4 cut(s) | 6, 1965, 2241, 2856 |
| EcoT38I | GRGCYC | 6 cut(s) | 1355, 1661, 1753, 2552, 3062, 3507 |
| ErhI | CCWWGG | 7 cut(s) | 1283, 1402, 1706, 2244, 4478, 4625, 4748 |
| Esp3I | CGTCTC | 1 cut(s) | 4596 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 249, 281, 2537, 2569 |
| FaqI | GGGAC | 4 cut(s) | 38, 1250, 2147, 2539 |
| FauI | CCCGC | 1 cut(s) | 669 |
| FauNDI | CATATG | 1 cut(s) | 4486 |
| FbaI | TGATCA | 4 cut(s) | 466, 1612, 1978, 3784 |
| FblI | GTMKAC | 3 cut(s) | 810, 2840, 4260 |
| FriOI | GRGCYC | 6 cut(s) | 1355, 1661, 1753, 2552, 3062, 3507 |
| GlaI | GCGC | 2 cut(s) | 2138, 3780 |
| GsuI | CTGGAG | 3 cut(s) | 1422, 2685, 4107 |
| HapII | CCGG | 7 cut(s) | 421, 886, 1504, 1746, 3255, 3583, 4016 |
| HgaI | GACGC | 2 cut(s) | 126, 3678 |
| HhaI | GCGC | 2 cut(s) | 2139, 3781 |
| Hin1I | GRCGYC | 1 cut(s) | 3670 |
| Hin6I | GCGC | 2 cut(s) | 2137, 3779 |
| HinP1I | GCGC | 2 cut(s) | 2137, 3779 |
| HincII | GTYRAC | 5 cut(s) | 48, 2044, 3134, 3850, 3918 |
| HindII | GTYRAC | 5 cut(s) | 48, 2044, 3134, 3850, 3918 |
| HindIII | AAGCTT | 3 cut(s) | 263, 389, 1394 |
| HpaI | GTTAAC | 1 cut(s) | 2044 |
| HpaII | CCGG | 7 cut(s) | 421, 886, 1504, 1746, 3255, 3583, 4016 |
| HphI | GGTGA | 8 cut(s) | 29, 146, 1022, 1455, 2103, 2421, 3172, 4605 |
| HpyCH4IV | ACGT | 3 cut(s) | 1210, 4367, 4605 |
| HpySE526I | ACGT | 3 cut(s) | 1210, 4367, 4605 |
| Hsp92I | GRCGYC | 1 cut(s) | 3670 |
| HspAI | GCGC | 2 cut(s) | 2137, 3779 |
| Kpn2I | TCCGGA | 1 cut(s) | 1745 |
| KpnI | GGTACC | 1 cut(s) | 2034 |
| KroI | GCCGGC | 1 cut(s) | 420 |
| KroNI | GCCGGC | 1 cut(s) | 422 |
| Ksp22I | TGATCA | 4 cut(s) | 466, 1612, 1978, 3784 |
| KspAI | GTTAAC | 1 cut(s) | 2044 |
| LguI | GCTCTTC | 1 cut(s) | 2127 |
| LmnI | GCTCC | 9 cut(s) | 673, 987, 1656, 1748, 2376, 2599, 3376, 4041, 4666 |
| MabI | ACCWGGT | 2 cut(s) | 2203, 4450 |
| MaeII | ACGT | 3 cut(s) | 1210, 4367, 4605 |
| MbiI | CCGCTC | 1 cut(s) | 676 |
| MfeI | CAATTG | 2 cut(s) | 2577, 4191 |
| MflI | RGATCY | 3 cut(s) | 1924, 2090, 3829 |
| MlyI | GAGTC | 8 cut(s) | 43, 88, 806, 2060, 2430, 2599, 2697, 2870 |
| MmeI | TCCRAC | 5 cut(s) | 2269, 2381, 2799, 3533, 4494 |
| Mph1103I | ATGCAT | 4 cut(s) | 6, 1965, 2241, 2856 |
| MroI | TCCGGA | 1 cut(s) | 1745 |
| MroNI | GCCGGC | 1 cut(s) | 420 |
| MslI | CAYNNNNRTG | 4 cut(s) | 324, 2849, 3087, 3400 |
| MspA1I | CMGCKG | 5 cut(s) | 2330, 3296, 3590, 3656, 4142 |
| MspI | CCGG | 7 cut(s) | 421, 886, 1504, 1746, 3255, 3583, 4016 |
| MunI | CAATTG | 2 cut(s) | 2577, 4191 |
| Mva1269I | GAATGC | 5 cut(s) | 1318, 1963, 2854, 3266, 4419 |
| MvnI | CGCG | 3 cut(s) | 368, 2139, 3781 |
| NaeI | GCCGGC | 1 cut(s) | 422 |
| NciI | CCSGG | 2 cut(s) | 1504, 3256 |
| NcoI | CCATGG | 1 cut(s) | 4625 |
| NdeI | CATATG | 1 cut(s) | 4486 |
| NgoMIV | GCCGGC | 1 cut(s) | 420 |
| NmuCI | GTSAC | 8 cut(s) | 743, 1034, 1461, 1522, 2109, 2602, 4144, 4606 |
| NsiI | ATGCAT | 4 cut(s) | 6, 1965, 2241, 2856 |
| NspI | RCATGY | 3 cut(s) | 46, 2243, 3197 |
| OliI | CACNNNNGTG | 1 cut(s) | 3087 |
| PaeI | GCATGC | 1 cut(s) | 2243 |
| PaeR7I | CTCGAG | 3 cut(s) | 522, 2451, 3716 |
| PaqCI | CACCTGC | 2 cut(s) | 2122, 4364 |
| PciI | ACATGT | 2 cut(s) | 42, 3193 |
| PciSI | GCTCTTC | 1 cut(s) | 2127 |
| PctI | GAATGC | 5 cut(s) | 1318, 1963, 2854, 3266, 4419 |
| PdiI | GCCGGC | 1 cut(s) | 422 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 4172 |
| PfoI | TCCNGGA | 1 cut(s) | 1502 |
| PleI | GAGTC | 8 cut(s) | 42, 88, 806, 2059, 2429, 2599, 2696, 2869 |
| PpsI | GAGTC | 8 cut(s) | 42, 88, 806, 2059, 2429, 2599, 2696, 2869 |
| PpuMI | RGGWCCY | 2 cut(s) | 2443, 4448 |
| PscI | ACATGT | 2 cut(s) | 42, 3193 |
| PshBI | ATTAAT | 2 cut(s) | 480, 1859 |
| Psp124BI | GAGCTC | 3 cut(s) | 1355, 1661, 1753 |
| Psp5II | RGGWCCY | 2 cut(s) | 2443, 4448 |
| PspEI | GGTNACC | 2 cut(s) | 1287, 2109 |
| PspOMI | GGGCCC | 2 cut(s) | 2548, 3503 |
| PspPPI | RGGWCCY | 2 cut(s) | 2443, 4448 |
| PspXI | VCTCGAGB | 1 cut(s) | 522 |
| PstI | CTGCAG | 4 cut(s) | 2158, 3055, 3280, 3496 |
| PstNI | CAGNNNCTG | 3 cut(s) | 2390, 3398, 4577 |
| PsuI | RGATCY | 3 cut(s) | 1924, 2090, 3829 |
| PvuII | CAGCTG | 3 cut(s) | 3590, 3656, 4142 |
| RsaI | GTAC | 3 cut(s) | 1993, 2032, 3699 |
| RsaNI | GTAC | 3 cut(s) | 1992, 2031, 3698 |
| RseI | CAYNNNNRTG | 4 cut(s) | 324, 2849, 3087, 3400 |
| SacI | GAGCTC | 3 cut(s) | 1355, 1661, 1753 |
| SapI | GCTCTTC | 1 cut(s) | 2127 |
| SchI | GAGTC | 8 cut(s) | 43, 88, 806, 2060, 2430, 2599, 2697, 2870 |
| SexAI | ACCWGGT | 2 cut(s) | 2203, 4450 |
| SfcI | CTRYAG | 6 cut(s) | 2154, 3051, 3276, 3492, 3651, 4261 |
| Sfr274I | CTCGAG | 3 cut(s) | 522, 2451, 3716 |
| SinI | GGWCC | 9 cut(s) | 581, 991, 2443, 3201, 3935, 3986, 4208, 4223, 4448 |
| SlaI | CTCGAG | 3 cut(s) | 522, 2451, 3716 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 324, 2849, 3087, 3400 |
| SmlI | CTYRAG | 6 cut(s) | 522, 651, 2438, 2451, 3716, 3732 |
| SmoI | CTYRAG | 6 cut(s) | 522, 651, 2438, 2451, 3716, 3732 |
| SphI | GCATGC | 1 cut(s) | 2243 |
| SsiI | CCGC | 7 cut(s) | 339, 676, 1147, 1908, 2328, 3294, 4695 |
| SspI | AATATT | 3 cut(s) | 1414, 3634, 3889 |
| SstI | GAGCTC | 3 cut(s) | 1355, 1661, 1753 |
| StyI | CCWWGG | 7 cut(s) | 1283, 1402, 1706, 2244, 4478, 4625, 4748 |
| TaiI | ACGT | 3 cut(s) | 1213, 4370, 4608 |
| TauI | GCSGC | 3 cut(s) | 341, 2330, 4698 |
| TseFI | GTSAC | 8 cut(s) | 743, 1034, 1461, 1522, 2109, 2602, 4144, 4606 |
| Tsp45I | GTSAC | 8 cut(s) | 743, 1034, 1461, 1522, 2109, 2602, 4144, 4606 |
| TspGWI | ACGGA | 4 cut(s) | 46, 129, 1123, 4421 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 4172 |
| VneI | GTGCAC | 1 cut(s) | 2913 |
| VpaK11BI | GGWCC | 9 cut(s) | 581, 991, 2443, 3201, 3935, 3986, 4208, 4223, 4448 |
| VspI | ATTAAT | 2 cut(s) | 480, 1859 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 4705, 4722 |
| XapI | RAATTY | 4 cut(s) | 303, 923, 3105, 3368 |
| XceI | RCATGY | 3 cut(s) | 46, 2243, 3197 |
| XhoI | CTCGAG | 3 cut(s) | 522, 2451, 3716 |
| XmaJI | CCTAGG | 1 cut(s) | 1706 |
| XmiI | GTMKAC | 3 cut(s) | 810, 2840, 4260 |
| Zsp2I | ATGCAT | 4 cut(s) | 6, 1965, 2241, 2856 |