Rh1AG254000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0018223
Physical Location & Seq
Forward (+)
47031142 .. 47031555
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG254000.1

Sequence Viewer

Length: 414 bp
ATGGAATGTGCTAATTTGGGGGAGACTGGTAATTCTTGTGCAGTGATTGCGCAATCTTTGTTGGTGCAGTCAATGGAAGGAGGAAATCCCATGATTGGGGATCTGAATTCAATGCGAACGCAGTCAGCGATAGAGGAGATTCTCCCTGCGTGGATCCCTAGTAATAAGTGGGATTGTACAGATCCTGATTTGAAGGGATTAAGCGGGAAAGCTAGTGGAAAGGCGTTGATTGGGATGGATGGTGTGAGTCACTCTAATTCAACCACGTCGAAGTCTGGGCCTGGGCCTAAAAAAAACAGTTCAGTGAGGGCCGCGGTTGATAGTGGAACTGAAGCCCATATGAAGAAACCTAGGGGGGCCAGAAACAGGCCGGTCTGTCCCCTGCAATTAGTAGGCCATCAAGGAAGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.33

Weight (kDa)

8.78

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018223)

Species Orthologous Gene IDs
rosa_laevigata RLG00000009410 RLG00000020622 RLG00000020623
rosa_multiflora Rmu_sc0006702.1_g000001
rosa_rugosa Rorug05G0215500
rosa_samantha Rh1AG254000 Rh1AG290000 Rh2CG265800 Rh5AG310600 Rh5BG130400 Rh6AG224100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 51
AccII CGCG 1 cut(s) 314
AciI CCGC 3 cut(s) 204, 312, 314
AclWI GGATC 4 cut(s) 108, 148, 161, 176
AcsI RAATTY 1 cut(s) 106
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 1 cut(s) 178
AfiI CCNNNNNNNGG 3 cut(s) 95, 96, 366
AgsI TTSAA 3 cut(s) 111, 193, 261
AjiI CACGTC 1 cut(s) 267
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 1 cut(s) 212
AluI AGCT 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 4 cut(s) 108, 148, 161, 176
AlwNI CAGNNNCTG 1 cut(s) 185
AoxI GGCC 6 cut(s) 278, 284, 309, 357, 368, 394
ApoI RAATTY 1 cut(s) 106
AspA2I CCTAGG 1 cut(s) 350
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 4 cut(s) 278, 284, 309, 357
AvrII CCTAGG 1 cut(s) 350
BamHI GGATCC 1 cut(s) 153
BccI CCATC 3 cut(s) 229, 233, 405
BciT130I CCWGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 4 cut(s) 159, 213, 351, 412
BisI GCNGC 1 cut(s) 312
BlnI CCTAGG 1 cut(s) 350
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 282
BmgBI CACGTC 1 cut(s) 267
BmgT120I GGNCC 4 cut(s) 278, 284, 309, 357
BmiI GGNNCC 2 cut(s) 155, 358
BmrFI CCNGG 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 281, 312, 350
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 96, 366
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 31
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 3 cut(s) 281, 312, 350
BseGI GGATG 2 cut(s) 240, 244
BseLI CCNNNNNNNGG 3 cut(s) 95, 96, 366
BseNI ACTGG 1 cut(s) 31
BseRI GAGGAG 1 cut(s) 149
BsgI GTGCAG 2 cut(s) 60, 86
Bsh1236I CGCG 1 cut(s) 314
BshFI GGCC 6 cut(s) 280, 286, 311, 359, 370, 396
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 3 cut(s) 95, 96, 366
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 6 cut(s) 280, 286, 311, 359, 370, 396
Bsp1407I TGTACA 1 cut(s) 176
Bsp143I GATC 3 cut(s) 100, 153, 181
BspACI CCGC 3 cut(s) 204, 312, 314
BspANI GGCC 6 cut(s) 280, 286, 311, 359, 370, 396
BspFNI CGCG 1 cut(s) 314
BspLI GGNNCC 2 cut(s) 155, 358
BspPI GGATC 4 cut(s) 108, 148, 161, 176
BsrFI RCCGGY 1 cut(s) 370
BsrGI TGTACA 1 cut(s) 176
BsrI ACTGG 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 3 cut(s) 281, 312, 350
BssMI GATC 3 cut(s) 100, 153, 181
BssT1I CCWWGG 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 176
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 2 cut(s) 240, 244
BstFNI CGCG 1 cut(s) 314
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 3 cut(s) 103, 156, 184
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 3 cut(s) 100, 153, 181
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 280
BstUI CGCG 1 cut(s) 314
BstX2I RGATCY 3 cut(s) 100, 153, 181
BstYI RGATCY 3 cut(s) 100, 153, 181
BsuRI GGCC 6 cut(s) 280, 286, 311, 359, 370, 396
BtgI CCRYGG 1 cut(s) 312
BtrI CACGTC 1 cut(s) 267
BtsCI GGATG 2 cut(s) 240, 244
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 309
CaiI CAGNNNCTG 1 cut(s) 185
CfoI GCGC 1 cut(s) 52
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 4 cut(s) 278, 284, 309, 357
Cfr42I CCGCGG 1 cut(s) 315
Csp6I GTAC 1 cut(s) 177
CviAII CATG 1 cut(s) 91
CviJI RGCY 8 cut(s) 212, 280, 286, 311, 335, 359, 370, 396
CviKI_1 RGCY 8 cut(s) 212, 280, 286, 311, 335, 359, 370, 396
CviQI GTAC 1 cut(s) 177
DpnI GATC 3 cut(s) 102, 155, 183
DpnII GATC 3 cut(s) 100, 153, 181
Eco130I CCWWGG 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 350
ErhI CCWWGG 1 cut(s) 350
FaeI CATG 1 cut(s) 94
FaiI YATR 3 cut(s) 92, 339, 341
FaqI GGGAC 1 cut(s) 363
FatI CATG 1 cut(s) 90
FauI CCCGC 1 cut(s) 197
FauNDI CATATG 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 2 cut(s) 247, 251
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 4 cut(s) 159, 213, 351, 412
FspI TGCGCA 1 cut(s) 51
GlaI GCGC 1 cut(s) 51
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 6 cut(s) 280, 286, 311, 359, 370, 396
HapII CCGG 1 cut(s) 371
HhaI GCGC 1 cut(s) 52
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HinfI GANTC 3 cut(s) 139, 247, 408
HpaII CCGG 1 cut(s) 371
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 1 cut(s) 185
Hpy99I CGWCG 1 cut(s) 271
HpyAV CCTTC 2 cut(s) 71, 187
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 3 cut(s) 41, 67, 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 266
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 1 cut(s) 50
KspI CCGCGG 1 cut(s) 315
Kzo9I GATC 3 cut(s) 100, 153, 181
MaeI CTAG 4 cut(s) 159, 213, 351, 412
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 1 cut(s) 248
MalI GATC 3 cut(s) 102, 155, 183
MboI GATC 3 cut(s) 100, 153, 181
MboII GAAGA 1 cut(s) 355
MflI RGATCY 3 cut(s) 100, 153, 181
MluCI AATT 5 cut(s) 13, 31, 106, 256, 386
MlyI GAGTC 1 cut(s) 256
MnlI CCTC 3 cut(s) 74, 127, 300
MseI TTAA 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 47
NdeI CATATG 1 cut(s) 339
NdeII GATC 3 cut(s) 100, 153, 181
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 2 cut(s) 155, 358
NmuCI GTSAC 1 cut(s) 248
NsbI TGCGCA 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PfeI GAWTC 2 cut(s) 139, 408
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 155, 358
PspPI GGNCC 4 cut(s) 278, 284, 309, 357
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 3 cut(s) 100, 153, 181
RsaI GTAC 1 cut(s) 178
RsaNI GTAC 1 cut(s) 177
SacII CCGCGG 1 cut(s) 315
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 3 cut(s) 100, 153, 181
Sau96I GGNCC 4 cut(s) 278, 284, 309, 357
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 282
SetI ASST 3 cut(s) 214, 269, 352
Sfr303I CCGCGG 1 cut(s) 315
SgrBI CCGCGG 1 cut(s) 315
Sse9I AATT 5 cut(s) 13, 31, 106, 256, 386
SsiI CCGC 3 cut(s) 204, 312, 314
SspMI CTAG 4 cut(s) 159, 213, 351, 412
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 1 cut(s) 269
TaqI TCGA 1 cut(s) 269
TasI AATT 5 cut(s) 13, 31, 106, 256, 386
TatI WGTACW 1 cut(s) 176
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 2 cut(s) 139, 408
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 2 cut(s) 48, 309
TseFI GTSAC 1 cut(s) 248
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 2 cut(s) 48, 309
XapI RAATTY 1 cut(s) 106
XmaJI CCTAGG 1 cut(s) 350
XspI CTAG 4 cut(s) 159, 213, 351, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.