Rh5BG130400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018223
Physical Location & Seq
Forward (+)
12859205 .. 12859860
656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG130400.1

Sequence Viewer

Length: 624 bp
ATGGAGGAGTTCTTGCTGCAGAAACGCTCTGAAGAAGCTAGAAACCTATCGCCGGAAACCACGGGGGTTGGTAGTAGATTTGGTTTGAAGCCGGTGAAGAAGACGGGGTGGTCGATGCAGGCACCCGAACTAACCATGTCGGGTCTAGTACGTTCTAGGGCGGAGTGGGAGCTGGAAGAGGTTCACGACGAACGGACGACTAGAGAGAGTGAGCAAATGATGATGGAGGTAGATAGAGAGGGACCGGAAGATGGGTTTCAGGCAGGAACGGTGGCTAAACGGCGATGTGTTAGGACGGAGAGGGATACCACGGCGGGGGGACAACTTTTGGAAGTTCCGATTACGGCGTGCATTAATGACAATTGCAGCTCTGCATTAATGACAATTACAGCTCTGCGTCAGACAGCATTAATGACAGCACAGGAGCCAGAAGCGCGTTTCACTAATGCGTCTGTATTGCCCATTTCACATGAAGCGGTAATTACGGAGCCAATGTCTGAAGCGGTAATCACGGAGCCAATGTCTGAAGCTGATAAAGGAGGAAAGTTTAATGGGAGAATCTCTTTTGAGACGACAACCACGGAGCAGTTTCCTAAAGTTGTTAAAGGAGTTAATCTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

22.95

Weight (kDa)

5.02

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018223)

Species Orthologous Gene IDs
rosa_laevigata RLG00000009410 RLG00000020622 RLG00000020623
rosa_multiflora Rmu_sc0006702.1_g000001
rosa_rugosa Rorug05G0215500
rosa_samantha Rh1AG254000 Rh1AG290000 Rh2CG265800 Rh5AG310600 Rh5BG130400 Rh6AG224100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 109
AccB1I GGYRCC 1 cut(s) 121
AccII CGCG 1 cut(s) 436
AciI CCGC 4 cut(s) 161, 314, 476, 503
AcuI CTGAAG 3 cut(s) 51, 519, 546
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 3 cut(s) 52, 251, 315
AgsI TTSAA 1 cut(s) 88
AluBI AGCT 5 cut(s) 38, 172, 369, 392, 530
AluI AGCT 5 cut(s) 38, 172, 369, 392, 530
Alw26I GTCTC 1 cut(s) 563
ApeKI GCWGC 2 cut(s) 16, 366
AseI ATTAAT 3 cut(s) 354, 377, 410
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 3, 378
BccI CCATC 2 cut(s) 217, 245
BceAI ACGGC 3 cut(s) 296, 327, 360
BciVI GTATCC 1 cut(s) 298
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 4 cut(s) 39, 146, 156, 201
BfmI CTRYAG 1 cut(s) 17
BfuI GTATCC 1 cut(s) 298
BisI GCNGC 2 cut(s) 17, 367
BlsI GCNGC 2 cut(s) 18, 368
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 5 cut(s) 123, 243, 426, 489, 516
BmsI GCATC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 60, 309, 579
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 479, 506, 509, 536
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 251, 315
Bse118I RCCGGY 1 cut(s) 91
BseDI CCNNGG 3 cut(s) 60, 309, 579
BseLI CCNNNNNNNGG 3 cut(s) 52, 251, 315
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 2 cut(s) 3, 378
Bsh1236I CGCG 1 cut(s) 436
BshNI GGYRCC 1 cut(s) 121
BsiSI CCGG 3 cut(s) 53, 92, 245
BslFI GGGAC 2 cut(s) 255, 333
BslI CCNNNNNNNGG 3 cut(s) 52, 251, 315
BsmAI GTCTC 1 cut(s) 563
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 2 cut(s) 255, 333
BspACI CCGC 4 cut(s) 161, 314, 476, 503
BspFNI CGCG 1 cut(s) 436
BspLI GGNNCC 5 cut(s) 123, 243, 426, 489, 516
BspMAI CTGCAG 1 cut(s) 21
BspT107I GGYRCC 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 60, 309, 579
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 120, 349
BstDSI CCRYGG 3 cut(s) 60, 309, 579
BstFNI CGCG 1 cut(s) 436
BstHHI GCGC 1 cut(s) 436
BstMAI GTCTC 1 cut(s) 563
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstSFI CTRYAG 1 cut(s) 17
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 2 cut(s) 3, 378
BstV2I GAAGAC 1 cut(s) 107
BsuI GTATCC 1 cut(s) 298
BtgI CCRYGG 3 cut(s) 60, 309, 579
BtgZI GCGATG 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 120, 349
CfoI GCGC 1 cut(s) 436
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 2 cut(s) 386, 438
Csp6I GTAC 1 cut(s) 149
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 2 cut(s) 136, 470
CviQI GTAC 1 cut(s) 149
DrdI GACNNNNNNGTC 1 cut(s) 109
DseDI GACNNNNNNGTC 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EciI GGCGGA 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 3 cut(s) 51, 519, 546
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 2 cut(s) 139, 473
FaiI YATR 2 cut(s) 137, 471
FaqI GGGAC 2 cut(s) 255, 333
FatI CATG 2 cut(s) 135, 469
FauI CCCGC 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 17, 367
Fsp4HI GCNGC 2 cut(s) 17, 367
FspBI CTAG 4 cut(s) 39, 146, 156, 201
GlaI GCGC 1 cut(s) 435
GluI GCNGC 2 cut(s) 17, 367
HapII CCGG 3 cut(s) 53, 92, 245
HgaI GACGC 2 cut(s) 386, 438
HhaI GCGC 1 cut(s) 436
Hin1II CATG 2 cut(s) 139, 473
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HinfI GANTC 1 cut(s) 558
HpaII CCGG 3 cut(s) 53, 92, 245
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 5 cut(s) 31, 339, 402, 499, 526
Hpy188III TCNNGA 1 cut(s) 185
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 1 cut(s) 151
HpyCH4V TGCA 6 cut(s) 19, 118, 351, 366, 374, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpySE526I ACGT 1 cut(s) 151
Hsp92II CATG 2 cut(s) 139, 473
HspAI GCGC 1 cut(s) 434
LmnI GCTCC 5 cut(s) 169, 424, 487, 514, 583
LpnPI CCDG 9 cut(s) 66, 104, 105, 158, 245, 249, 258, 407, 441
Lsp1109I GCAGC 2 cut(s) 3, 378
LweI GCATC 1 cut(s) 105
MaeI CTAG 4 cut(s) 39, 146, 156, 201
MaeII ACGT 1 cut(s) 151
MboII GAAGA 5 cut(s) 44, 109, 112, 188, 260
MfeI CAATTG 1 cut(s) 361
MluCI AATT 3 cut(s) 361, 384, 480
MnlI CCTC 5 cut(s) 172, 220, 232, 294, 533
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 7 cut(s) 354, 377, 410, 549, 603, 612, 622
MspI CCGG 3 cut(s) 53, 92, 245
MunI CAATTG 1 cut(s) 361
MvnI CGCG 1 cut(s) 436
MwoI GCNNNNNNNGC 1 cut(s) 433
NlaIII CATG 2 cut(s) 139, 473
NlaIV GGNNCC 5 cut(s) 123, 243, 426, 489, 516
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 1 cut(s) 558
PkrI GCNGC 2 cut(s) 18, 368
PshBI ATTAAT 3 cut(s) 354, 377, 410
PspN4I GGNNCC 5 cut(s) 123, 243, 426, 489, 516
PspPI GGNCC 1 cut(s) 242
PstI CTGCAG 1 cut(s) 21
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
SaqAI TTAA 7 cut(s) 354, 377, 410, 549, 603, 612, 622
SatI GCNGC 2 cut(s) 17, 367
Sau96I GGNCC 1 cut(s) 242
SetI ASST 9 cut(s) 40, 48, 154, 174, 183, 231, 371, 394, 532
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 1 cut(s) 17
SinI GGWCC 1 cut(s) 242
Sse9I AATT 3 cut(s) 361, 384, 480
SsiI CCGC 4 cut(s) 161, 314, 476, 503
SspMI CTAG 4 cut(s) 39, 146, 156, 201
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 113
TasI AATT 3 cut(s) 361, 384, 480
TfiI GAWTC 1 cut(s) 558
Tru1I TTAA 7 cut(s) 354, 377, 410, 549, 603, 612, 622
Tru9I TTAA 7 cut(s) 354, 377, 410, 549, 603, 612, 622
TseI GCWGC 2 cut(s) 16, 366
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 5 cut(s) 208, 311, 500, 527, 596
VpaK11BI GGWCC 1 cut(s) 242
VspI ATTAAT 3 cut(s) 354, 377, 410
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 4 cut(s) 39, 146, 156, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.