Rh1AG443200

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014504
Physical Location & Seq
Forward (+)
66417993 .. 66419305
1313 bp
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UTR
Exon/CDS
Intron
Rh1AG443200.1

Sequence Viewer

Length: 636 bp
ATGGGAGAGAGGCTGCGATTGGCAACTGGGGTGATGGGAAATGCTGCTTCTTTGTTGCTTTACTCGACTCCCATATTGACTATGTCAAGGATCACAAGGAAGAAAAGTACAGAGGAGTTTTCATGTGTACCTTACATCACTGCATTGCTCAACTGTCTCCTTTATACTTGGTATGGATTGCCTGTTGTAAGCTGTGGCTGGGAAAACTTTCCAGTGGTTACCATCAATGGTCTAGGAATTCTTCTTGAGTTCTCATTCATTCTCATATACTTCTGGTTTGCTTCACCTACAAGAAAGATGAAGGTAATTGCAATTCTAATACCTGTTATGGTCATGTTCTGCATCACCGTTAGCATCTCAACCTTTGTTTTCCATGACCACCGTCATCAAAAGGAATTTGTTGGAAGTCTAGGGCTGGTGGCCTCTGTAACAATGTATGCTTCTCCACTGGTAGCTGTGTCGCCTAATCTGGTTGGAAGCCCATTAGGAATATTTCAACTACTGCTCTACTGCAAGTACAGGAAAAGGGAAATGAAAACTGTGGAATCACCAAACAACTGGGATGTTGAAGAGAATGATGAAAAATCTAAACAGCTGCAGATTGTGATTAGTGAAAGTGCAAATGGCAAAAGTTGA

Protein Analysis

211

Amino Acids

23.69

Weight (kDa)

8.79

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 10 - 95 3.6e-14 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0014504)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53190
fragaria_vesca FvH4_7g31080 FvH4_7g31080 FvH4_7g31080
malus_domestica MD01G1215700.v1.1
prunus_persica Prupe.2G307700_v2.0.a1 Prupe.2G307800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0380151
rosa_laevigata RLG00000026326
rosa_roxburghii Rroxscaffold_4G00279630
rosa_rugosa Rorug01G0418200 Rorug01G0418300 Rorug01G0418400
rosa_samantha Rh1AG443200 Rh1BG398100 Rh1DG428900
rosa_wichuraiana Rw1G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 2 cut(s) 237, 395
AfaI GTAC 3 cut(s) 109, 129, 518
AgsI TTSAA 2 cut(s) 497, 569
AluBI AGCT 3 cut(s) 192, 455, 595
AluI AGCT 3 cut(s) 192, 455, 595
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 98
AoxI GGCC 1 cut(s) 420
ApeKI GCWGC 3 cut(s) 13, 44, 595
ApoI RAATTY 2 cut(s) 237, 395
Asp700I GAANNNNTTC 1 cut(s) 207
AsuHPI GGTGA 4 cut(s) 43, 276, 337, 540
BbvI GCAGC 2 cut(s) 31, 582
BccI CCATC 2 cut(s) 28, 230
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 233, 410
BfmI CTRYAG 1 cut(s) 596
BisI GCNGC 3 cut(s) 14, 45, 596
BlsI GCNGC 3 cut(s) 15, 46, 597
BmrI ACTGGG 2 cut(s) 36, 568
BmsI GCATC 2 cut(s) 351, 363
BmuI ACTGGG 2 cut(s) 36, 568
BoxI GACNNNNGTC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 266
Bse1I ACTGG 4 cut(s) 31, 212, 453, 563
Bse3DI GCAATG 1 cut(s) 143
BseGI GGATG 1 cut(s) 568
BseMI GCAATG 1 cut(s) 143
BseNI ACTGG 4 cut(s) 31, 212, 453, 563
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 2 cut(s) 31, 582
BseYI CCCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 1 cut(s) 422
Bsp143I GATC 1 cut(s) 90
BspANI GGCC 1 cut(s) 422
BspMAI CTGCAG 1 cut(s) 600
BspPI GGATC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 143
BsrI ACTGG 4 cut(s) 31, 212, 453, 563
BssMI GATC 1 cut(s) 90
Bst4CI ACNGT 4 cut(s) 155, 349, 383, 541
Bst6I CTCTTC 1 cut(s) 564
BstEII GGTNACC 1 cut(s) 217
BstF5I GGATG 1 cut(s) 568
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 1 cut(s) 90
BstPAI GACNNNNGTC 1 cut(s) 381
BstPI GGTNACC 1 cut(s) 217
BstSFI CTRYAG 1 cut(s) 596
BstV1I GCAGC 2 cut(s) 31, 582
BstXI CCANNNNNNTGG 1 cut(s) 558
BsuRI GGCC 1 cut(s) 422
BtsCI GGATG 1 cut(s) 568
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 3 cut(s) 138, 219, 446
Csp6I GTAC 3 cut(s) 108, 128, 517
CviAII CATG 3 cut(s) 123, 334, 374
CviJI RGCY 8 cut(s) 13, 192, 198, 415, 422, 455, 480, 595
CviKI_1 RGCY 8 cut(s) 13, 192, 198, 415, 422, 455, 480, 595
CviQI GTAC 3 cut(s) 108, 128, 517
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Eco91I GGTNACC 1 cut(s) 217
EcoO65I GGTNACC 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 237
FaeI CATG 3 cut(s) 126, 337, 377
FatI CATG 3 cut(s) 122, 333, 373
Fnu4HI GCNGC 3 cut(s) 14, 45, 596
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 3 cut(s) 14, 45, 596
FspBI CTAG 2 cut(s) 233, 410
GluI GCNGC 3 cut(s) 14, 45, 596
GsaI CCCAGC 1 cut(s) 202
HaeIII GGCC 1 cut(s) 422
Hin1II CATG 3 cut(s) 126, 337, 377
HinfI GANTC 2 cut(s) 67, 545
HphI GGTGA 4 cut(s) 43, 276, 337, 540
Hpy166II GTNNAC 1 cut(s) 128
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 4 cut(s) 155, 349, 383, 541
HpyCH4V TGCA 6 cut(s) 143, 311, 342, 513, 598, 620
Hsp92II CATG 3 cut(s) 126, 337, 377
Kzo9I GATC 1 cut(s) 90
Lsp1109I GCAGC 2 cut(s) 31, 582
LweI GCATC 2 cut(s) 351, 363
MaeI CTAG 2 cut(s) 233, 410
MaeIII GTNAC 2 cut(s) 217, 427
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 3 cut(s) 112, 233, 581
MluCI AATT 4 cut(s) 237, 306, 312, 395
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 2 cut(s) 382, 454
MnlI CCTC 3 cut(s) 3, 106, 433
MroXI GAANNNNTTC 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 595
NdeII GATC 1 cut(s) 90
NlaIII CATG 3 cut(s) 126, 337, 377
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 545
PflFI GACNNNGTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 15, 46, 597
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
PshAI GACNNNNGTC 1 cut(s) 381
PspEI GGTNACC 1 cut(s) 217
PspFI CCCAGC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 600
PsyI GACNNNGTC 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 595
RsaI GTAC 3 cut(s) 109, 129, 518
RsaNI GTAC 3 cut(s) 108, 128, 517
SatI GCNGC 3 cut(s) 14, 45, 596
Sau3AI GATC 1 cut(s) 90
SchI GAGTC 1 cut(s) 61
SetI ASST 8 cut(s) 133, 194, 289, 306, 325, 365, 457, 597
SfaNI GCATC 2 cut(s) 351, 363
SfcI CTRYAG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 4 cut(s) 237, 306, 312, 395
SspI AATATT 1 cut(s) 492
SspMI CTAG 2 cut(s) 233, 410
TaaI ACNGT 4 cut(s) 155, 349, 383, 541
TaqI TCGA 1 cut(s) 65
TasI AATT 4 cut(s) 237, 306, 312, 395
TatI WGTACW 2 cut(s) 107, 516
TfiI GAWTC 1 cut(s) 545
TscAI CASTG 3 cut(s) 145, 219, 453
TseI GCWGC 3 cut(s) 13, 44, 595
TspDTI ATGAA 5 cut(s) 111, 247, 314, 548, 594
TspRI CASTG 3 cut(s) 145, 219, 453
Tth111I GACNNNGTC 1 cut(s) 82
XapI RAATTY 2 cut(s) 237, 395
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 2 cut(s) 233, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.