Rh1DG428900

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014504
Physical Location & Seq
Forward (+)
64133732 .. 64138893
5162 bp
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UTR
Exon/CDS
Intron
Rh1DG428900.1

Sequence Viewer

Length: 474 bp
ATGGGAGAGAGGCTGCGATTGGCAACTGGGGTGATGGGAAATGCTGCTTCTTTGTTGCTTTACTCGACTCCCGTATTGACTATGTCAAGGATCACAAGGAAGAAAAGTACAGAGGAGTTTTCATGTGTACCTTACATCACTGCATTGCTCAACTGTCTCCTTTATACTTGGTATGGATTGCCTGTTGTAAGCTGTGGCTGGGAAAACTTTCCAGTGGTTACCATCAATGGTCTAGGAATTCTTCTTGAGTTCTCATTCATTCTCATATACTTCTGGTTTGCTTCACCTACAAGAAAGATGAAGGTAATTGCAATTCTGATACCTGTTATGGTCATGTTCTGCATCACCGTTAGCATCTCAACCTTTGTTTTCCATGACCACCGTCATCGAAAGGAATTTGTTGGAAGTCTAGGGCTGGTGGCCTCTGTAACAATGTATGCTTCTCCACTGGTAGCTGTGGTAAGCTATTCTTGA

Protein Analysis

157

Amino Acids

17.54

Weight (kDa)

9.18

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 10 - 95 1.5e-14 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014504)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53190
fragaria_vesca FvH4_7g31080 FvH4_7g31080 FvH4_7g31080
malus_domestica MD01G1215700.v1.1
prunus_persica Prupe.2G307700_v2.0.a1 Prupe.2G307800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0380151
rosa_laevigata RLG00000026326
rosa_roxburghii Rroxscaffold_4G00279630
rosa_rugosa Rorug01G0418200 Rorug01G0418300 Rorug01G0418400
rosa_samantha Rh1AG443200 Rh1BG398100 Rh1DG428900
rosa_wichuraiana Rw1G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 2 cut(s) 237, 395
AfaI GTAC 2 cut(s) 109, 129
AluBI AGCT 3 cut(s) 192, 455, 465
AluI AGCT 3 cut(s) 192, 455, 465
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 98
AoxI GGCC 1 cut(s) 420
ApeKI GCWGC 2 cut(s) 13, 44
ApoI RAATTY 2 cut(s) 237, 395
Asp700I GAANNNNTTC 1 cut(s) 207
AsuHPI GGTGA 3 cut(s) 43, 276, 337
BbvI GCAGC 1 cut(s) 31
BccI CCATC 2 cut(s) 28, 230
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 233, 410
BisI GCNGC 2 cut(s) 14, 45
BlsI GCNGC 2 cut(s) 15, 46
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 2 cut(s) 351, 363
BmuI ACTGGG 1 cut(s) 36
BoxI GACNNNNGTC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 31, 212, 453
Bse3DI GCAATG 1 cut(s) 143
BseMI GCAATG 1 cut(s) 143
BseNI ACTGG 3 cut(s) 31, 212, 453
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 1 cut(s) 422
Bsp143I GATC 1 cut(s) 90
BspANI GGCC 1 cut(s) 422
BspPI GGATC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 143
BsrI ACTGG 3 cut(s) 31, 212, 453
BssMI GATC 1 cut(s) 90
Bst4CI ACNGT 3 cut(s) 155, 349, 383
BstEII GGTNACC 1 cut(s) 217
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 1 cut(s) 90
BstPAI GACNNNNGTC 1 cut(s) 381
BstPI GGTNACC 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 31
BsuRI GGCC 1 cut(s) 422
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 3 cut(s) 138, 219, 446
Csp6I GTAC 2 cut(s) 108, 128
CviAII CATG 3 cut(s) 123, 334, 374
CviJI RGCY 7 cut(s) 13, 192, 198, 415, 422, 455, 465
CviKI_1 RGCY 7 cut(s) 13, 192, 198, 415, 422, 455, 465
CviQI GTAC 2 cut(s) 108, 128
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
Eco91I GGTNACC 1 cut(s) 217
EcoO65I GGTNACC 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 237
FaeI CATG 3 cut(s) 126, 337, 377
FalI AAGNNNNNCTT 1 cut(s) 454
FatI CATG 3 cut(s) 122, 333, 373
Fnu4HI GCNGC 2 cut(s) 14, 45
Fsp4HI GCNGC 2 cut(s) 14, 45
FspBI CTAG 2 cut(s) 233, 410
GluI GCNGC 2 cut(s) 14, 45
GsaI CCCAGC 1 cut(s) 202
HaeIII GGCC 1 cut(s) 422
Hin1II CATG 3 cut(s) 126, 337, 377
HinfI GANTC 1 cut(s) 67
HphI GGTGA 3 cut(s) 43, 276, 337
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 2 cut(s) 245, 471
Hpy8I GTNNAC 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 3 cut(s) 155, 349, 383
HpyCH4V TGCA 3 cut(s) 143, 311, 342
Hsp92II CATG 3 cut(s) 126, 337, 377
Kzo9I GATC 1 cut(s) 90
LpnPI CCDG 8 cut(s) 12, 184, 195, 225, 259, 336, 401, 434
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 2 cut(s) 351, 363
MaeI CTAG 2 cut(s) 233, 410
MaeIII GTNAC 2 cut(s) 217, 427
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 2 cut(s) 112, 233
MluCI AATT 4 cut(s) 237, 306, 312, 395
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 382
MnlI CCTC 3 cut(s) 3, 106, 433
MroXI GAANNNNTTC 1 cut(s) 207
NdeII GATC 1 cut(s) 90
NlaIII CATG 3 cut(s) 126, 337, 377
PdmI GAANNNNTTC 1 cut(s) 207
PflFI GACNNNGTC 1 cut(s) 82
PkrI GCNGC 2 cut(s) 15, 46
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
PshAI GACNNNNGTC 1 cut(s) 381
PspEI GGTNACC 1 cut(s) 217
PspFI CCCAGC 1 cut(s) 198
PsyI GACNNNGTC 1 cut(s) 82
RsaI GTAC 2 cut(s) 109, 129
RsaNI GTAC 2 cut(s) 108, 128
SatI GCNGC 2 cut(s) 14, 45
Sau3AI GATC 1 cut(s) 90
SchI GAGTC 1 cut(s) 61
SetI ASST 8 cut(s) 133, 194, 289, 306, 325, 365, 457, 467
SfaNI GCATC 2 cut(s) 351, 363
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 4 cut(s) 237, 306, 312, 395
SspMI CTAG 2 cut(s) 233, 410
TaaI ACNGT 3 cut(s) 155, 349, 383
TaqI TCGA 2 cut(s) 65, 388
TasI AATT 4 cut(s) 237, 306, 312, 395
TatI WGTACW 1 cut(s) 107
TscAI CASTG 3 cut(s) 145, 219, 453
TseI GCWGC 2 cut(s) 13, 44
TspDTI ATGAA 3 cut(s) 111, 247, 314
TspRI CASTG 3 cut(s) 145, 219, 453
Tth111I GACNNNGTC 1 cut(s) 82
XapI RAATTY 2 cut(s) 237, 395
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 2 cut(s) 233, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.