Rh1DG060700

agmatine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0018980
Physical Location & Seq
Forward (+)
10610593 .. 10618555
7963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG060700.1

Sequence Viewer

Length: 243 bp
ATGCCGCCTATATCTCTAGGTGCCAAGCTATCCGCCCGCCCTGACGACCTCGGGCTTTTTTTATTTGAAGCAAGCTTTGCAGGCACAAGACTTGCAGCTTCTTATGTGAATTTCTACGTTGCCAATGGAGCAATCATTGCGCCACAATTTGGGGACCAAAAATGGGACGATGAAGCTGTCCGAGTCCTATCGAAAGCCTTTCCATACCATGAAAACCATGCTACTTTATTTAAGATTTCATGA

Protein Analysis

80

Amino Acids

8.77

Weight (kDa)

6.02

Isoelectric Point (pI)

20.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAD_porph PF04371 27 - 71 4.9e-14 Porphyromonas-type peptidyl-arginine deiminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 149
AciI CCGC 3 cut(s) 5, 33, 37
AcsI RAATTY 1 cut(s) 109
AfiI CCNNNNNNNGG 2 cut(s) 149, 163
AgsI TTSAA 1 cut(s) 68
AluBI AGCT 4 cut(s) 28, 75, 98, 176
AluI AGCT 4 cut(s) 28, 75, 98, 176
Ama87I CYCGRG 1 cut(s) 50
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 1 cut(s) 109
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 154
BanI GGYRCC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 107
BfaI CTAG 1 cut(s) 17
BisI GCNGC 2 cut(s) 5, 96
BlsI GCNGC 2 cut(s) 6, 97
Bme18I GGWCC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 2 cut(s) 22, 155
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 163
Bse3DI GCAATG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 49
BseLI CCNNNNNNNGG 2 cut(s) 149, 163
BseMI GCAATG 1 cut(s) 135
BseXI GCAGC 1 cut(s) 107
BshNI GGYRCC 1 cut(s) 20
BsiHKCI CYCGRG 1 cut(s) 50
BslFI GGGAC 2 cut(s) 167, 179
BslI CCNNNNNNNGG 2 cut(s) 149, 163
BsmFI GGGAC 2 cut(s) 167, 179
BsoBI CYCGRG 1 cut(s) 50
BspACI CCGC 3 cut(s) 5, 33, 37
BspHI TCATGA 1 cut(s) 239
BspLI GGNNCC 2 cut(s) 22, 155
BspT107I GGYRCC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 49
BstAPI GCANNNNNTGC 2 cut(s) 77, 137
BstC8I GCNNGC 3 cut(s) 37, 73, 82
BstHHI GCGC 1 cut(s) 142
BstMWI GCNNNNNNNGC 4 cut(s) 77, 81, 128, 137
BstV1I GCAGC 1 cut(s) 107
Cac8I GCNNGC 3 cut(s) 37, 73, 82
CciI TCATGA 1 cut(s) 239
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 154
CviAII CATG 3 cut(s) 209, 218, 240
CviJI RGCY 6 cut(s) 28, 55, 75, 98, 176, 197
CviKI_1 RGCY 6 cut(s) 28, 55, 75, 98, 176, 197
EciI GGCGGA 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 50
FaeI CATG 3 cut(s) 212, 221, 243
FaiI YATR 6 cut(s) 11, 105, 205, 210, 219, 241
FaqI GGGAC 2 cut(s) 167, 179
FatI CATG 3 cut(s) 208, 217, 239
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 5, 96
Fsp4HI GCNGC 2 cut(s) 5, 96
FspBI CTAG 1 cut(s) 17
GlaI GCGC 1 cut(s) 141
GluI GCNGC 2 cut(s) 5, 96
HhaI GCGC 1 cut(s) 142
Hin1II CATG 3 cut(s) 212, 221, 243
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HindIII AAGCTT 1 cut(s) 73
HinfI GANTC 1 cut(s) 183
Hpy188I TCNGA 1 cut(s) 182
Hpy188III TCNNGA 1 cut(s) 240
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 2 cut(s) 80, 95
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 81, 128, 137
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 3 cut(s) 212, 221, 243
HspAI GCGC 1 cut(s) 140
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 2 cut(s) 54, 66
Lsp1109I GCAGC 1 cut(s) 107
MaeI CTAG 1 cut(s) 17
MaeII ACGT 1 cut(s) 117
MluCI AATT 2 cut(s) 109, 146
MlyI GAGTC 1 cut(s) 192
MnlI CCTC 1 cut(s) 59
MseI TTAA 1 cut(s) 231
MwoI GCNNNNNNNGC 4 cut(s) 77, 81, 128, 137
NlaIII CATG 3 cut(s) 212, 221, 243
NlaIV GGNNCC 2 cut(s) 22, 155
PagI TCATGA 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 149
PkrI GCNGC 2 cut(s) 6, 97
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PspN4I GGNNCC 2 cut(s) 22, 155
PspPI GGNCC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 5, 96
Sau96I GGNCC 1 cut(s) 154
SchI GAGTC 1 cut(s) 192
SetI ASST 7 cut(s) 22, 30, 51, 77, 100, 120, 178
SinI GGWCC 1 cut(s) 154
Sse9I AATT 2 cut(s) 109, 146
SsiI CCGC 3 cut(s) 5, 33, 37
SspMI CTAG 1 cut(s) 17
TaiI ACGT 1 cut(s) 120
TaqI TCGA 1 cut(s) 191
TasI AATT 2 cut(s) 109, 146
TauI GCSGC 1 cut(s) 7
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseI GCWGC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 186, 225, 228
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 154
XapI RAATTY 1 cut(s) 109
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.