Rh3CG071000

agmatine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0018980
Physical Location & Seq
Reverse (-)
4966404 .. 4993607
27204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG071000.1

Sequence Viewer

Length: 345 bp
ATGGATGATGCTAAACCGAGACTTGCAGGCACAAGACTTGCAGCTTCTTATGTGAATTTCTACATTGCTAATGGAGCAATCATTGCGCCACAATTTGGGGACCAAAAATGGGATGATGAGGTAGTCCGAGTCCTATCGAAAGCCTTTCCATACCATGAAGAGGTCTTTCCAATCAATAGCTTCTTGGCCAATCAAGACCAAACTCTACCTTTGCCAAAAGTACGAATGCAAAGTGCTCTCTCTCTTAAAAAGAAGGGAATCACACATAGGAGACGTATGGATTTTAGTAGATCCATATATGATGGGTTTCTAAAGGTTTTCCATAAAAGGCGTTTAACTAATTAG

Protein Analysis

114

Amino Acids

13.17

Weight (kDa)

10.05

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAD_porph PF04371 7 - 59 1.1e-13 Porphyromonas-type peptidyl-arginine deiminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AclWI GGATC 1 cut(s) 285
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 55
AfaI GTAC 1 cut(s) 222
AfiI CCNNNNNNNGG 3 cut(s) 95, 109, 160
AluBI AGCT 2 cut(s) 44, 180
AluI AGCT 2 cut(s) 44, 180
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 2 cut(s) 13, 265
AlwI GGATC 1 cut(s) 285
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 41
ApoI RAATTY 1 cut(s) 55
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 100
AvaII GGWCC 1 cut(s) 100
BalI TGGCCA 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 53
BccI CCATC 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 13, 265
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 1 cut(s) 101
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 109, 160
Bse3DI GCAATG 2 cut(s) 63, 81
BseGI GGATG 2 cut(s) 10, 118
BseLI CCNNNNNNNGG 3 cut(s) 95, 109, 160
BseMI GCAATG 2 cut(s) 63, 81
BseXI GCAGC 1 cut(s) 53
BshFI GGCC 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 238
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 3 cut(s) 95, 109, 160
BsmAI GTCTC 2 cut(s) 13, 265
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 1 cut(s) 290
BspANI GGCC 1 cut(s) 188
BspLI GGNNCC 1 cut(s) 101
BspPI GGATC 1 cut(s) 285
BsrDI GCAATG 2 cut(s) 63, 81
BssMI GATC 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 28
BstF5I GGATG 2 cut(s) 10, 118
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 1 cut(s) 293
BstMAI GTCTC 2 cut(s) 13, 265
BstMBI GATC 1 cut(s) 290
BstMWI GCNNNNNNNGC 2 cut(s) 74, 83
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 2 cut(s) 10, 118
Cac8I GCNNGC 1 cut(s) 28
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 221
CviAII CATG 1 cut(s) 155
CviJI RGCY 4 cut(s) 44, 143, 180, 188
CviKI_1 RGCY 4 cut(s) 44, 143, 180, 188
CviQI GTAC 1 cut(s) 221
DpnI GATC 1 cut(s) 292
DpnII GATC 1 cut(s) 290
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 1 cut(s) 158
FaiI YATR 9 cut(s) 51, 151, 156, 267, 278, 296, 298, 300, 324
FaqI GGGAC 1 cut(s) 113
FatI CATG 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 2 cut(s) 17, 125
Fsp4HI GCNGC 1 cut(s) 42
GlaI GCGC 1 cut(s) 87
GluI GCNGC 1 cut(s) 42
HaeIII GGCC 1 cut(s) 188
HhaI GCGC 1 cut(s) 88
Hin1II CATG 1 cut(s) 158
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 2 cut(s) 129, 258
Hpy188I TCNGA 1 cut(s) 128
Hpy188III TCNNGA 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 274
HpyCH4V TGCA 3 cut(s) 26, 41, 229
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 83
HpySE526I ACGT 1 cut(s) 274
Hsp92II CATG 1 cut(s) 158
HspAI GCGC 1 cut(s) 86
Kzo9I GATC 1 cut(s) 290
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 1 cut(s) 12
Lsp1109I GCAGC 1 cut(s) 53
MaeII ACGT 1 cut(s) 274
MalI GATC 1 cut(s) 292
MboI GATC 1 cut(s) 290
MboII GAAGA 1 cut(s) 170
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 3 cut(s) 55, 92, 340
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 1 cut(s) 138
MnlI CCTC 2 cut(s) 112, 154
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 246, 335
Msp20I TGGCCA 1 cut(s) 188
Mva1269I GAATGC 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 74, 83
NdeII GATC 1 cut(s) 290
NlaIII CATG 1 cut(s) 158
NlaIV GGNNCC 1 cut(s) 101
PctI GAATGC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 258
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 1 cut(s) 43
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 100
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
SaqAI TTAA 2 cut(s) 246, 335
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 1 cut(s) 290
Sau96I GGNCC 1 cut(s) 100
SchI GAGTC 1 cut(s) 138
SduI GDGCHC 1 cut(s) 238
SetI ASST 7 cut(s) 46, 123, 165, 182, 211, 277, 318
SgeI CNNG 9 cut(s) 30, 35, 39, 45, 50, 140, 167, 196, 206
SinI GGWCC 1 cut(s) 100
Sse9I AATT 3 cut(s) 55, 92, 340
TaiI ACGT 1 cut(s) 277
TaqI TCGA 1 cut(s) 137
TasI AATT 3 cut(s) 55, 92, 340
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 2 cut(s) 246, 335
Tru9I TTAA 2 cut(s) 246, 335
TseI GCWGC 1 cut(s) 41
TspDTI ATGAA 1 cut(s) 171
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.