Rh7AG285400

agmatine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0018980
Physical Location & Seq
Forward (+)
30554831 .. 30600467
45637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG285400.1

Sequence Viewer

Length: 702 bp
ATGGCTCTGTGGTTGGGACCAACTCAGCTTTTAGTGTCAATAAAGGATCCAACTCTTATCAGAGTCATGCTACTAAAGGCTGCAAATAAGTTGCCTATGATTGGGAGGGCATTCCATTTGGCCTTTGGAAGGTCAAGTCTCTTTGCTTATTCTTTGGATGAGGATGAAGAGACCATCCTCAATGAATTCCTTATTGAACGTGGAGTACAATGTGATTTGGTGTTGATGGTAATGCTATCATCCATAAAGCCAGATAAGTACTGGACCGCTCACTCATTCAAGAATCAGATTTGGAGTTCCAAAAGCTTAAGTGATCTTTTTGAGCAAATGAGGCAGGATGATGCTAAACCGAGACTTGCAGGCACAAGACTTGCAGCTTCTTTTGTGAATTTCTACGTTGCCAATGGAGCAATCATTGCGCCACAATTTGGGGACCAAAAATGGGACGATGAGGCTGTCCGAGTCCTATCGAAAGCCTTTCCATACCATGAAGAGGTCTTTCCAATCAATAGCTTCTTGGCCAATCAAGACCAAACTTTGCCAAAAGTGCGAATGCAAAGTGCTCTCTCTCTTAAAAAGAAGGTTGCTGATAAACTGTTTGCTGCAAAAAACTACCTTCCAAAAGCCTTACTTGAAATCACGAGCGTGGACATGTTTAAATCATTAGGGTTTCATCGCTGTATCTTTTCTCAAGTCTGCTGA

Protein Analysis

233

Amino Acids

26.43

Weight (kDa)

8.9

Isoelectric Point (pI)

25.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAD_porph PF04371 116 - 170 5.1e-13 Porphyromonas-type peptidyl-arginine deiminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 428
AccBSI CCGCTC 1 cut(s) 269
AciI CCGC 1 cut(s) 267
AclWI GGATC 2 cut(s) 41, 54
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 2 cut(s) 185, 388
AfaI GTAC 2 cut(s) 207, 260
AfiI CCNNNNNNNGG 5 cut(s) 101, 129, 428, 442, 493
AflII CTTAAG 1 cut(s) 307
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 3 cut(s) 197, 280, 635
AleI CACNNNNGTG 1 cut(s) 644
AluBI AGCT 4 cut(s) 28, 306, 377, 513
AluI AGCT 4 cut(s) 28, 306, 377, 513
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 3 cut(s) 143, 164, 346
AlwI GGATC 2 cut(s) 41, 54
AoxI GGCC 2 cut(s) 120, 519
ApeKI GCWGC 3 cut(s) 80, 374, 602
ApoI RAATTY 2 cut(s) 185, 388
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 3 cut(s) 17, 264, 433
AvaII GGWCC 3 cut(s) 17, 264, 433
BalI TGGCCA 1 cut(s) 521
BamHI GGATCC 1 cut(s) 46
BauI CACGAG 1 cut(s) 640
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 3 cut(s) 67, 386, 589
BccI CCATC 2 cut(s) 182, 220
BcoDI GTCTC 3 cut(s) 143, 164, 346
BfrI CTTAAG 1 cut(s) 307
BisI GCNGC 3 cut(s) 81, 375, 603
BlsI GCNGC 3 cut(s) 82, 376, 604
BmcAI AGTACT 1 cut(s) 260
Bme18I GGWCC 3 cut(s) 17, 264, 433
BmgT120I GGNCC 3 cut(s) 17, 264, 433
BmiI GGNNCC 3 cut(s) 18, 48, 434
BmsI GCATC 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 675
BsaI GGTCTC 1 cut(s) 164
Bsc4I CCNNNNNNNGG 5 cut(s) 101, 129, 428, 442, 493
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 414
BseGI GGATG 5 cut(s) 163, 169, 174, 239, 343
BseLI CCNNNNNNNGG 5 cut(s) 101, 129, 428, 442, 493
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 266
BseXI GCAGC 3 cut(s) 67, 386, 589
BshFI GGCC 2 cut(s) 122, 521
BsiHKAI GWGCWC 1 cut(s) 565
BslFI GGGAC 3 cut(s) 30, 446, 458
BslI CCNNNNNNNGG 5 cut(s) 101, 129, 428, 442, 493
BsmAI GTCTC 3 cut(s) 143, 164, 346
BsmFI GGGAC 3 cut(s) 30, 446, 458
BsmI GAATGC 2 cut(s) 110, 558
BsnI GGCC 2 cut(s) 122, 521
Bso31I GGTCTC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 565
Bsp143I GATC 2 cut(s) 46, 313
BspACI CCGC 1 cut(s) 267
BspANI GGCC 2 cut(s) 122, 521
BspCNI CTCAG 1 cut(s) 37
BspLI GGNNCC 3 cut(s) 18, 48, 434
BspPI GGATC 2 cut(s) 41, 54
BspTI CTTAAG 1 cut(s) 307
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 1 cut(s) 266
BssMI GATC 2 cut(s) 46, 313
BssSI CACGAG 1 cut(s) 640
Bst2BI CACGAG 1 cut(s) 640
Bst4CI ACNGT 1 cut(s) 597
Bst6I CTCTTC 2 cut(s) 162, 486
BstAFI CTTAAG 1 cut(s) 307
BstAPI GCANNNNNTGC 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 1 cut(s) 24
BstENI CCTNNNNNAGG 1 cut(s) 127
BstF5I GGATG 5 cut(s) 163, 169, 174, 239, 343
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 2 cut(s) 49, 316
BstMAI GTCTC 3 cut(s) 143, 164, 346
BstMBI GATC 2 cut(s) 46, 313
BstMWI GCNNNNNNNGC 4 cut(s) 331, 407, 416, 547
BstNSI RCATGY 1 cut(s) 655
BstV1I GCAGC 3 cut(s) 67, 386, 589
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 2 cut(s) 122, 521
BtgZI GCGATG 1 cut(s) 659
BtsCI GGATG 5 cut(s) 163, 169, 174, 239, 343
Cac8I GCNNGC 1 cut(s) 361
CfoI GCGC 1 cut(s) 421
Cfr13I GGNCC 3 cut(s) 17, 264, 433
Csp6I GTAC 2 cut(s) 206, 259
CviAII CATG 3 cut(s) 67, 488, 652
CviQI GTAC 2 cut(s) 206, 259
DdeI CTNAG 1 cut(s) 24
DpnI GATC 2 cut(s) 48, 315
DpnII GATC 2 cut(s) 46, 313
DraI TTTAAA 1 cut(s) 658
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 162, 486
EarI CTCTTC 2 cut(s) 162, 486
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 3 cut(s) 17, 264, 433
EcoNI CCTNNNNNAGG 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 185
FaeI CATG 3 cut(s) 70, 491, 655
FaiI YATR 6 cut(s) 68, 98, 245, 484, 489, 653
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 3 cut(s) 30, 446, 458
FatI CATG 3 cut(s) 66, 487, 651
Fnu4HI GCNGC 3 cut(s) 81, 375, 603
FokI GGATG 5 cut(s) 161, 170, 176, 226, 350
Fsp4HI GCNGC 3 cut(s) 81, 375, 603
GlaI GCGC 1 cut(s) 420
GluI GCNGC 3 cut(s) 81, 375, 603
HaeIII GGCC 2 cut(s) 122, 521
HhaI GCGC 1 cut(s) 421
Hin1II CATG 3 cut(s) 70, 491, 655
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 3 cut(s) 63, 283, 462
Hpy166II GTNNAC 1 cut(s) 649
Hpy188I TCNGA 3 cut(s) 62, 288, 461
Hpy188III TCNNGA 3 cut(s) 280, 527, 640
Hpy8I GTNNAC 1 cut(s) 649
HpyAV CCTTC 3 cut(s) 123, 574, 626
HpyCH4III ACNGT 1 cut(s) 597
HpyCH4IV ACGT 2 cut(s) 199, 396
HpyCH4V TGCA 5 cut(s) 83, 359, 374, 556, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 331, 407, 416, 547
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 2 cut(s) 199, 396
Hsp92II CATG 3 cut(s) 70, 491, 655
HspAI GCGC 1 cut(s) 419
Kzo9I GATC 2 cut(s) 46, 313
LmnI GCTCC 1 cut(s) 407
LpnPI CCDG 4 cut(s) 247, 264, 320, 345
Lsp1109I GCAGC 3 cut(s) 67, 386, 589
LweI GCATC 1 cut(s) 331
MaeII ACGT 2 cut(s) 199, 396
MalI GATC 2 cut(s) 48, 315
MbiI CCGCTC 1 cut(s) 269
MboI GATC 2 cut(s) 46, 313
MboII GAAGA 2 cut(s) 179, 503
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 565
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 3 cut(s) 185, 388, 425
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 2 cut(s) 72, 471
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 6 cut(s) 99, 154, 188, 324, 445, 487
Mox20I TGGCCA 1 cut(s) 521
MscI TGGCCA 1 cut(s) 521
MseI TTAA 3 cut(s) 308, 573, 657
MslI CAYNNNNRTG 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 521
MspCI CTTAAG 1 cut(s) 307
Mva1269I GAATGC 2 cut(s) 110, 558
MwoI GCNNNNNNNGC 4 cut(s) 331, 407, 416, 547
NdeII GATC 2 cut(s) 46, 313
NlaIII CATG 3 cut(s) 70, 491, 655
NlaIV GGNNCC 3 cut(s) 18, 48, 434
NspI RCATGY 1 cut(s) 655
OliI CACNNNNGTG 1 cut(s) 644
PciI ACATGT 1 cut(s) 651
PctI GAATGC 2 cut(s) 110, 558
PfeI GAWTC 1 cut(s) 283
PflMI CCANNNNNTGG 1 cut(s) 428
PkrI GCNGC 3 cut(s) 82, 376, 604
PleI GAGTC 2 cut(s) 71, 470
PpsI GAGTC 2 cut(s) 71, 470
PscI ACATGT 1 cut(s) 651
PspN4I GGNNCC 3 cut(s) 18, 48, 434
PspPI GGNCC 3 cut(s) 17, 264, 433
PsrI GAACNNNNNNTAC 2 cut(s) 189, 221
PsuI RGATCY 1 cut(s) 46
RsaI GTAC 2 cut(s) 207, 260
RsaNI GTAC 2 cut(s) 206, 259
RseI CAYNNNNRTG 1 cut(s) 644
SaqAI TTAA 3 cut(s) 308, 573, 657
SatI GCNGC 3 cut(s) 81, 375, 603
Sau3AI GATC 2 cut(s) 46, 313
Sau96I GGNCC 3 cut(s) 17, 264, 433
ScaI AGTACT 1 cut(s) 260
SchI GAGTC 2 cut(s) 72, 471
SduI GDGCHC 1 cut(s) 565
SfaNI GCATC 1 cut(s) 331
SinI GGWCC 3 cut(s) 17, 264, 433
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 307, 690
SmoI CTYRAG 2 cut(s) 307, 690
Sse9I AATT 3 cut(s) 185, 388, 425
SsiI CCGC 1 cut(s) 267
TaaI ACNGT 1 cut(s) 597
TaiI ACGT 2 cut(s) 202, 399
TaqI TCGA 1 cut(s) 470
TasI AATT 3 cut(s) 185, 388, 425
TatI WGTACW 2 cut(s) 205, 258
TfiI GAWTC 1 cut(s) 283
Tru1I TTAA 3 cut(s) 308, 573, 657
Tru9I TTAA 3 cut(s) 308, 573, 657
TseI GCWGC 3 cut(s) 80, 374, 602
TspDTI ATGAA 4 cut(s) 180, 198, 504, 662
Van91I CCANNNNNTGG 1 cut(s) 428
Vha464I CTTAAG 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 17, 264, 433
XagI CCTNNNNNAGG 1 cut(s) 127
XapI RAATTY 2 cut(s) 185, 388
XceI RCATGY 1 cut(s) 655
XcmI CCANNNNNNNNNTGG 2 cut(s) 122, 258
ZrmI AGTACT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.