Rh2DG361100

agmatine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018980
Physical Location & Seq
Reverse (-)
51031366 .. 51043794
12429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG361100.1

Sequence Viewer

Length: 294 bp
ATGGTAGTGGGTAGGAACATAGATTTCATCACGGTTGCAGGCACAAGACTTGCAGCTTCTTATGTGAATTTCTATGTTGCCAATGGAGCAATCATTGCTCCACAATTTGGGGACCAAAAATGGGACGATGAGGCTGTCCGAGTCCTATCGAAAGCCTTTCCATACCATGAAGAGGTCTTTCCAATCAATAGGTTCTTGGCCAATCAAGACCAAACTTTGCCAAAAGTGCGAATGCAAAGTGCTCTCTCTCTTAAAAAGAAGATCCATGGATTTTACTTGCGAATTGAGTTGTGA

Protein Analysis

97

Amino Acids

11.06

Weight (kDa)

9.4

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAD_porph PF04371 13 - 62 3.9e-14 Porphyromonas-type peptidyl-arginine deiminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 1 cut(s) 67
AfiI CCNNNNNNNGG 3 cut(s) 107, 121, 172
AluBI AGCT 1 cut(s) 56
AluI AGCT 1 cut(s) 56
Alw21I GWGCWC 1 cut(s) 244
AlwI GGATC 1 cut(s) 256
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 67
AspS9I GGNCC 1 cut(s) 112
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 1 cut(s) 65
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 121, 172
Bse3DI GCAATG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 265
BseLI CCNNNNNNNGG 3 cut(s) 107, 121, 172
BseMI GCAATG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 65
BshFI GGCC 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 244
BslFI GGGAC 2 cut(s) 125, 137
BslI CCNNNNNNNGG 3 cut(s) 107, 121, 172
BsmFI GGGAC 2 cut(s) 125, 137
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 1 cut(s) 261
Bsp19I CCATGG 1 cut(s) 265
BspANI GGCC 1 cut(s) 200
BspLI GGNNCC 1 cut(s) 113
BspPI GGATC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 165
BstAPI GCANNNNNTGC 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 40
BstDSI CCRYGG 1 cut(s) 265
BstKTI GATC 1 cut(s) 264
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 3 cut(s) 86, 95, 226
BstV1I GCAGC 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 1 cut(s) 200
BtgI CCRYGG 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 112
CviAII CATG 2 cut(s) 167, 266
CviJI RGCY 4 cut(s) 56, 134, 155, 200
CviKI_1 RGCY 4 cut(s) 56, 134, 155, 200
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 2 cut(s) 170, 269
FaiI YATR 6 cut(s) 20, 63, 75, 163, 168, 267
FaqI GGGAC 2 cut(s) 125, 137
FatI CATG 2 cut(s) 166, 265
Fnu4HI GCNGC 1 cut(s) 54
Fsp4HI GCNGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 54
HaeIII GGCC 1 cut(s) 200
Hin1II CATG 2 cut(s) 170, 269
HinfI GANTC 1 cut(s) 141
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4V TGCA 3 cut(s) 38, 53, 235
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 95, 226
Hsp92II CATG 2 cut(s) 170, 269
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 2 cut(s) 86, 103
LpnPI CCDG 1 cut(s) 24
Lsp1109I GCAGC 1 cut(s) 65
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 2 cut(s) 182, 271
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 244
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 3 cut(s) 67, 104, 282
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 1 cut(s) 150
MnlI CCTC 2 cut(s) 124, 166
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
MseI TTAA 1 cut(s) 252
Msp20I TGGCCA 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 86, 95, 226
NcoI CCATGG 1 cut(s) 265
NdeII GATC 1 cut(s) 261
NlaIII CATG 2 cut(s) 170, 269
NlaIV GGNNCC 1 cut(s) 113
PctI GAATGC 1 cut(s) 237
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 1 cut(s) 261
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 1 cut(s) 150
SduI GDGCHC 1 cut(s) 244
SetI ASST 3 cut(s) 58, 177, 194
SinI GGWCC 1 cut(s) 112
Sse9I AATT 3 cut(s) 67, 104, 282
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 34
TaqI TCGA 1 cut(s) 149
TasI AATT 3 cut(s) 67, 104, 282
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TseI GCWGC 1 cut(s) 53
TspDTI ATGAA 2 cut(s) 16, 183
Van91I CCANNNNNTGG 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.