Rh1DG268400

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0015360
Physical Location & Seq
Forward (+)
48481077 .. 48481646
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG268400.1

Sequence Viewer

Length: 570 bp
ATGTCCCCCCCTCCCTCCTCCCCCTCCTCAAATTACCTCACTACCCTCGGCCTCGGCTACGCCATAGCCATCGCCCTTGGCTTTCTCGTCCTTCTCTCCACCCTCCTCCTCGCCTCCTACATCTGCTGCCGCGTCTCCCGCCACCACCGCTCCCAGCCCCAGCCCCAAACCCAAAACCCCAACTTCTCCGAGGGCGTCATCCTCCCCAGAATCATCTTCGTCGCCGAGGACGACATCGAGAACCAAGAAAACGACGATGAGAACTCCGTCGTCGGCCTCCACCAGAACGTCATCAACTCCTACCCCAAGTTCCCCTTCTCCAAGGAAATCGGCGGCGGCGATGCCTCCACGTGCTCCATCTGCCTCTGCGACTACCGCGAATTCGAGATGCTGAGGATGATGCCCGAGTGCCGCCACCACTTCCACCTATTGTGTCTCGACGCGTGGCTCAAGCTCAACGGCTCCTGCCCCGTCTGCCGGAACTCGCCGCTGCCGACTCCGCTGTCGACGCCGCTGCAGGAGGTGGTGCCGCTTAGCCAGCACGCCGCCGATCGGAGATGGAGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

189

Amino Acids

21.18

Weight (kDa)

6.14

Isoelectric Point (pI)

71.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 117 - 160 3.6e-12 Ring finger domain
zf-RING_11 PF17123 118 - 146 1.2e-06 RING-like zinc finger
zf-rbx1 PF12678 130 - 160 6.6e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015360)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46160 AT2G46160
fragaria_vesca FvH4_7g15500
malus_domestica MD01G1072300.v1.1
prunus_persica Prupe.2G178600_v2.0.a1
pyrus_communis pycom01g10150 pycom07g12760
rosa_chinensis RchiOBHm_Chr1g0357831
rosa_laevigata RLG00000027993
rosa_multiflora Rmu_sc0002071.1_g000001 Rmu_sc0011109.1_g000007
rosa_samantha Rh1AG272800 Rh1BG240100 Rh1CG256700 Rh1DG268400
rosa_wichuraiana Rw1G024350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 502
AccB1I GGYRCC 1 cut(s) 526
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 506
AccII CGCG 3 cut(s) 132, 378, 443
AcsI RAATTY 1 cut(s) 380
AcvI CACGTG 1 cut(s) 351
AcyI GRCGYC 2 cut(s) 195, 509
AfiI CCNNNNNNNGG 2 cut(s) 477, 552
AflIII ACRYGT 1 cut(s) 441
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 1 cut(s) 454
AluI AGCT 1 cut(s) 454
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 2 cut(s) 139, 440
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 2 cut(s) 49, 274
ApeKI GCWGC 3 cut(s) 126, 490, 514
ApoI RAATTY 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 404
BanI GGYRCC 1 cut(s) 526
BbrPI CACGTG 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 356
BbvCI CCTCAGC 1 cut(s) 392
BbvI GCAGC 3 cut(s) 113, 477, 501
BccI CCATC 3 cut(s) 77, 365, 552
BceAI ACGGC 1 cut(s) 475
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BcoDI GTCTC 2 cut(s) 139, 440
BfmI CTRYAG 1 cut(s) 515
BlpI GCTNAGC 1 cut(s) 533
BmeT110I CYCGRG 1 cut(s) 404
BmiI GGNNCC 2 cut(s) 463, 528
BmsI GCATC 3 cut(s) 331, 378, 390
Bpu10I CCTNAGC 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 434
BsaAI YACGTR 1 cut(s) 351
BsaHI GRCGYC 2 cut(s) 195, 509
BsaJI CCNNGG 6 cut(s) 46, 52, 76, 189, 225, 321
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 477, 552
BseDI CCNNGG 6 cut(s) 46, 52, 76, 189, 225, 321
BseGI GGATG 2 cut(s) 198, 402
BseLI CCNNNNNNNGG 2 cut(s) 477, 552
BseMII CTCAG 1 cut(s) 383
BseRI GAGGAG 4 cut(s) 7, 16, 95, 98
BseXI GCAGC 3 cut(s) 113, 477, 501
BseYI CCCAGC 2 cut(s) 153, 159
Bsh1236I CGCG 3 cut(s) 132, 378, 443
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 2 cut(s) 51, 276
BshNI GGYRCC 1 cut(s) 526
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 356
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 478
BslI CCNNNNNNNGG 2 cut(s) 477, 552
BsmAI GTCTC 2 cut(s) 139, 440
BsmBI CGTCTC 1 cut(s) 139
BsnI GGCC 2 cut(s) 51, 276
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 1 cut(s) 550
Bsp1720I GCTNAGC 1 cut(s) 533
BspANI GGCC 2 cut(s) 51, 276
BspCNI CTCAG 1 cut(s) 384
BspFNI CGCG 3 cut(s) 132, 378, 443
BspLI GGNNCC 2 cut(s) 463, 528
BspMAI CTGCAG 1 cut(s) 519
BspT107I GGYRCC 1 cut(s) 526
BsrBI CCGCTC 1 cut(s) 150
BssECI CCNNGG 6 cut(s) 46, 52, 76, 189, 225, 321
BssMI GATC 1 cut(s) 550
BssNI GRCGYC 2 cut(s) 195, 509
BssT1I CCWWGG 2 cut(s) 76, 321
BstACI GRCGYC 2 cut(s) 195, 509
BstBAI YACGTR 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 539, 543
BstDEI CTNAG 2 cut(s) 392, 533
BstF5I GGATG 2 cut(s) 198, 402
BstFNI CGCG 3 cut(s) 132, 378, 443
BstKTI GATC 1 cut(s) 553
BstMAI GTCTC 2 cut(s) 139, 440
BstMBI GATC 1 cut(s) 550
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 8 cut(s) 138, 147, 360, 375, 474, 499, 508, 538
BstSFI CTRYAG 1 cut(s) 515
BstUI CGCG 3 cut(s) 132, 378, 443
BstV1I GCAGC 3 cut(s) 113, 477, 501
BsuRI GGCC 2 cut(s) 51, 276
BtgZI GCGATG 2 cut(s) 55, 354
BtsCI GGATG 2 cut(s) 198, 402
Cac8I GCNNGC 2 cut(s) 539, 543
CseI GACGC 4 cut(s) 121, 184, 449, 517
DdeI CTNAG 2 cut(s) 392, 533
DpnI GATC 1 cut(s) 552
DpnII GATC 1 cut(s) 550
DrdI GACNNNNNNGTC 1 cut(s) 502
DseDI GACNNNNNNGTC 1 cut(s) 502
Eco130I CCWWGG 2 cut(s) 76, 321
Eco72I CACGTG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 380
EcoT14I CCWWGG 2 cut(s) 76, 321
ErhI CCWWGG 2 cut(s) 76, 321
Esp3I CGTCTC 1 cut(s) 139
FaiI YATR 1 cut(s) 65
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FauI CCCGC 1 cut(s) 146
FblI GTMKAC 1 cut(s) 506
FokI GGATG 2 cut(s) 185, 409
GsaI CCCAGC 2 cut(s) 157, 163
HaeIII GGCC 2 cut(s) 51, 276
HapII CCGG 1 cut(s) 478
HgaI GACGC 4 cut(s) 121, 184, 449, 517
Hin1I GRCGYC 2 cut(s) 195, 509
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 2 cut(s) 210, 496
HpaII CCGG 1 cut(s) 478
Hpy166II GTNNAC 1 cut(s) 507
Hpy188I TCNGA 2 cut(s) 190, 555
Hpy188III TCNNGA 3 cut(s) 238, 385, 437
Hpy8I GTNNAC 1 cut(s) 507
Hpy99I CGWCG 6 cut(s) 224, 257, 272, 275, 443, 511
HpyAV CCTTC 2 cut(s) 101, 325
HpyCH4IV ACGT 2 cut(s) 288, 350
HpyCH4V TGCA 1 cut(s) 517
HpyF10VI GCNNNNNNNGC 8 cut(s) 138, 147, 360, 375, 474, 499, 508, 538
HpyF3I CTNAG 2 cut(s) 392, 533
HpySE526I ACGT 2 cut(s) 288, 350
Hsp92I GRCGYC 2 cut(s) 195, 509
Kzo9I GATC 1 cut(s) 550
LmnI GCTCC 3 cut(s) 155, 359, 467
LpnPI CCDG 8 cut(s) 167, 173, 220, 296, 478, 491, 503, 551
Lsp1109I GCAGC 3 cut(s) 113, 477, 501
LweI GCATC 3 cut(s) 331, 378, 390
MaeII ACGT 2 cut(s) 288, 350
MalI GATC 1 cut(s) 552
MbiI CCGCTC 1 cut(s) 150
MboI GATC 1 cut(s) 550
MboII GAAGA 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 2 cut(s) 31, 380
MluI ACGCGT 1 cut(s) 441
MlyI GAGTC 1 cut(s) 490
MspA1I CMGCKG 3 cut(s) 490, 502, 514
MspI CCGG 1 cut(s) 478
MvnI CGCG 3 cut(s) 132, 378, 443
MwoI GCNNNNNNNGC 8 cut(s) 138, 147, 360, 375, 474, 499, 508, 538
NdeII GATC 1 cut(s) 550
NlaIV GGNNCC 2 cut(s) 463, 528
NmeAIII GCCGAG 3 cut(s) 27, 33, 250
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 336, 491
PfeI GAWTC 1 cut(s) 210
Ple19I CGATCG 1 cut(s) 553
PleI GAGTC 1 cut(s) 490
PmaCI CACGTG 1 cut(s) 351
PmlI CACGTG 1 cut(s) 351
PpsI GAGTC 1 cut(s) 490
Ppu21I YACGTR 1 cut(s) 351
PspCI CACGTG 1 cut(s) 351
PspFI CCCAGC 2 cut(s) 153, 159
PspN4I GGNNCC 2 cut(s) 463, 528
PstI CTGCAG 1 cut(s) 519
PvuI CGATCG 1 cut(s) 553
SalI GTCGAC 1 cut(s) 505
Sau3AI GATC 1 cut(s) 550
SchI GAGTC 1 cut(s) 490
SduI GDGCHC 1 cut(s) 356
SetI ASST 7 cut(s) 39, 291, 353, 429, 456, 525, 569
SfaNI GCATC 3 cut(s) 331, 378, 390
SfcI CTRYAG 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 2 cut(s) 31, 380
StyI CCWWGG 2 cut(s) 76, 321
TaiI ACGT 2 cut(s) 291, 353
TaqI TCGA 4 cut(s) 237, 384, 438, 506
TasI AATT 2 cut(s) 31, 380
TauI GCSGC 8 cut(s) 132, 336, 339, 414, 490, 514, 532, 548
TfiI GAWTC 1 cut(s) 210
TseI GCWGC 3 cut(s) 126, 490, 514
TspGWI ACGGA 1 cut(s) 256
XapI RAATTY 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.