Rh2AG264700

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018565
Physical Location & Seq
Forward (+)
30838834 .. 30841350
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG264700.1

Sequence Viewer

Length: 1560 bp
ATGATTCGACATGTGTCAATATCTAAAAAGGACTTGATTGGAGAAGACGCACAAGTTCCCGAATTCATGCTTAATTCAAAGAAATTGCGAACCATTATAGTTCTTAAAGAAGGTATGATGAGTGAACGTTTTGTGAAGACATGCATCTCGAGATTCAAATGTATGCGGGTGCTAGATCTCAGTGGGTCGCCTCTTGGGGAGCTACCAAATTCCATTGGTAGTTTGTTTCATTTGAGATTCCTCAACTTGTCTGATAATAGAAAGATAAAAAGGCTTCCCAATTCCATCAGTAAGATGCTGAATTTGGAGACCTTGTCCTTTTGGGGTTGTGAGGCACTTGAGGAGATACCCAAAGACATAGGAAACCTAATCAACCTCCGGAGCTTGGAGATAACTACACAACAAACTTATTTGCCAAAAGGAATTAGACGGCTCACCTTACTTCAATATTTAGATTTTGAGGGATGTGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCGACAACCTTCGTAAACTGATGATTAGCAGGTGTAATAATTTGGAATGCTTGCCACCAAATATGAAACACATGACTGCTTTAGATACTTTGGGTGTCAATGATTGCGAGAACCTTCAGTTGATGATGAGATCAGGGGAAGATCCTCAAGGTCTTCGATCATTGATTATCGAGAAATCAAGTTTGGAGGCTTTGCCCCCTTGGCTTACTGAAGATTCTGCAGACACTCTACAGAGCATATGGCTTGGTCGATGTGATAATCTCACGACACTTTCGGAGTTAATAAAGTTCAGATTCCTTGAGCAACTACAAATTGAAGAATGCTCCAAATTGTCGGTTTTGCCGCAAGGGTTGCATTGCCTTACTGAGTTGAGAGGATTGAAGATTTCAGGGTGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAAGATGGCACGTCAGGTGAAGATTACACTCGACGCTGATGATGAAGAAGATGAATCCAACAATCGCTTGGCCGACGGAGGAGATGGAGGTGTCAGAGAGGCTGGAGAGGGTGTAAAAGGCGTTGGAGAGTGTTGTAGAGGATATGGACTTGATGGGGAGGCTTTGGATGAGCTTGATAAGCTTCAGAATCGGTCGCAGGAGTATGACTTGAGTATGGCGGATACAAAGATTACTGTTGATCAGAGATACATGCTTTATCTTCAGGACCCTGATTTACTGCTGCTGGACGAGCCTACAAATTACCTTTACCTTGACATAATTGAGTGGCTCGAAGATTATTTCAATCAGCAGGCTGTGCCAATGGTTATCATATCTCATGACCGAGCTTTTCTCGATGAATTTTGTACAAAAATAGTGGAAACTGATATGCGTGTCTCCAGGACGTATGAGGGAAATTATTGTATTATATTTGCAAAGGCAGCATGGATTGAAAGTCAAAATGCTGCTTGGGAGATGGAGAAGCAGCAGAAGGAAAACGAGCACACAAAAGACTTGATACACAAGTTGGGCGATGGAACAAATTCTGGCCGTGCATCTTCTGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

519

Amino Acids

59.06

Weight (kDa)

5.01

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 47 - 292 7e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 217 - 276 1.8e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018565)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0116711
rosa_roxburghii Rroxscaffold_7G00160560
rosa_samantha Rh2AG264700 Rh2BG275600 Rh2BG278400 Rh2DG272000 Rh2DG290000
rosa_wichuraiana Rw2G020750 Rw2G020780 Rw2G020830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 525
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 3 cut(s) 166, 848, 1169
AclI AACGTT 1 cut(s) 127
AclWI GGATC 2 cut(s) 490, 641
AcoI YGGCCR 2 cut(s) 1020, 1537
AcsI RAATTY 5 cut(s) 62, 208, 301, 1349, 1531
AcuI CTGAAG 4 cut(s) 605, 735, 1118, 1196
AfaI GTAC 1 cut(s) 1357
AfiI CCNNNNNNNGG 1 cut(s) 385
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 7 cut(s) 78, 157, 446, 821, 886, 1294, 1442
AjiI CACGTC 1 cut(s) 962
AjnI CCWGG 1 cut(s) 1388
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AluBI AGCT 6 cut(s) 202, 384, 918, 1123, 1132, 1337
AluI AGCT 6 cut(s) 202, 384, 918, 1123, 1132, 1337
Alw21I GWGCWC 1 cut(s) 1494
Alw26I GTCTC 2 cut(s) 302, 1390
AlwI GGATC 2 cut(s) 490, 641
Ama87I CYCGRG 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 378
AoxI GGCC 2 cut(s) 1020, 1537
ApeKI GCWGC 5 cut(s) 918, 1231, 1430, 1454, 1474
ApoI RAATTY 5 cut(s) 62, 208, 301, 1349, 1531
Asp700I GAANNNNTTC 1 cut(s) 1531
AspS9I GGNCC 1 cut(s) 1216
AsuHPI GGTGA 3 cut(s) 427, 950, 979
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 1216
BaeI ACNNNNGTAYC 2 cut(s) 1499, 1532
BbsI GAAGAC 3 cut(s) 51, 143, 650
Bbv12I GWGCWC 1 cut(s) 1494
BbvI GCAGC 5 cut(s) 905, 1218, 1441, 1442, 1486
BccI CCATC 6 cut(s) 293, 949, 1028, 1097, 1459, 1517
BceAI ACGGC 2 cut(s) 446, 1524
BciT130I CCWGG 1 cut(s) 1390
BciVI GTATCC 1 cut(s) 1165
BclI TGATCA 1 cut(s) 1189
BcoDI GTCTC 2 cut(s) 302, 1390
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 2 cut(s) 723, 734
BfuAI ACCTGC 1 cut(s) 525
BfuI GTATCC 1 cut(s) 1165
BglI GCCNNNNNGGC 1 cut(s) 706
BglII AGATCT 1 cut(s) 175
BisI GCNGC 6 cut(s) 848, 919, 1232, 1431, 1455, 1475
BlsI GCNGC 6 cut(s) 849, 920, 1233, 1432, 1456, 1476
Bme1390I CCNGG 1 cut(s) 1390
Bme18I GGWCC 1 cut(s) 1216
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 962
BmgT120I GGNCC 1 cut(s) 1216
BmiI GGNNCC 1 cut(s) 1218
BmrFI CCNGG 1 cut(s) 1390
BmsI GCATC 3 cut(s) 153, 285, 1553
BoxI GACNNNNGTC 1 cut(s) 13
BpiI GAAGAC 3 cut(s) 51, 143, 650
BplI GAGNNNNNCTC 2 cut(s) 1326, 1358
BpmI CTGGAG 2 cut(s) 1074, 1372
BpuEI CTTGAG 4 cut(s) 359, 636, 824, 1180
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 704
BsaWI WCCGGW 1 cut(s) 378
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 1047, 1077
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 859
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 1 cut(s) 1390
BseDI CCNNGG 1 cut(s) 704
BseGI GGATG 2 cut(s) 470, 1123
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMI GCAATG 1 cut(s) 859
BseMII CTCAG 2 cut(s) 193, 861
BseRI GAGGAG 3 cut(s) 356, 956, 1044
BseXI GCAGC 5 cut(s) 905, 1218, 1441, 1442, 1486
Bsh1285I CGRYCG 1 cut(s) 1145
BshFI GGCC 2 cut(s) 1022, 1539
BsiEI CGRYCG 1 cut(s) 1145
BsiHKAI GWGCWC 1 cut(s) 1494
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 2 cut(s) 302, 1390
BsmI GAATGC 2 cut(s) 557, 830
BsnI GGCC 2 cut(s) 1022, 1539
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 1494
Bsp13I TCCGGA 1 cut(s) 378
Bsp1407I TGTACA 1 cut(s) 1355
Bsp143I GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
BspACI CCGC 3 cut(s) 166, 848, 1169
BspANI GGCC 2 cut(s) 1022, 1539
BspCNI CTCAG 2 cut(s) 192, 862
BspEI TCCGGA 1 cut(s) 378
BspHI TCATGA 1 cut(s) 1327
BspLI GGNNCC 1 cut(s) 1218
BspMAI CTGCAG 1 cut(s) 727
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 2 cut(s) 490, 641
BspTNI GGTCTC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 859
BsrGI TGTACA 1 cut(s) 1355
BssECI CCNNGG 1 cut(s) 704
BssMI GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
BssT1I CCWWGG 1 cut(s) 704
Bst2UI CCWGG 1 cut(s) 1390
Bst4CI ACNGT 1 cut(s) 1186
Bst6I CTCTTC 1 cut(s) 486
BstAPI GCANNNNNTGC 4 cut(s) 856, 918, 1306, 1550
BstAUI TGTACA 1 cut(s) 1355
BstC8I GCNNGC 2 cut(s) 557, 1302
BstDEI CTNAG 2 cut(s) 179, 870
BstF5I GGATG 2 cut(s) 470, 1123
BstKTI GATC 6 cut(s) 178, 498, 638, 649, 665, 1192
BstMAI GTCTC 2 cut(s) 302, 1390
BstMBI GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
BstMCI CGRYCG 1 cut(s) 1145
BstMWI GCNNNNNNNGC 8 cut(s) 706, 856, 918, 1129, 1240, 1306, 1430, 1550
BstNI CCWGG 1 cut(s) 1390
BstNSI RCATGY 3 cut(s) 14, 144, 1204
BstPAI GACNNNNGTC 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 1388
BstSFI CTRYAG 2 cut(s) 723, 734
BstV1I GCAGC 5 cut(s) 905, 1218, 1441, 1442, 1486
BstV2I GAAGAC 3 cut(s) 51, 143, 650
BstX2I RGATCY 3 cut(s) 175, 495, 646
BstYI RGATCY 3 cut(s) 175, 495, 646
BsuI GTATCC 1 cut(s) 1165
BsuRI GGCC 2 cut(s) 1022, 1539
BtgZI GCGATG 1 cut(s) 1536
BtrI CACGTC 1 cut(s) 962
BtsCI GGATG 2 cut(s) 470, 1123
BtsIMutI CAGTG 1 cut(s) 187
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 2 cut(s) 557, 1302
CciI TCATGA 1 cut(s) 1327
Cfr13I GGNCC 1 cut(s) 1216
CseI GACGC 2 cut(s) 56, 992
Csp6I GTAC 1 cut(s) 1356
CspCI CAANNNNNGTGG 2 cut(s) 1347, 1382
CviAII CATG 7 cut(s) 11, 67, 141, 577, 1201, 1328, 1434
CviQI GTAC 1 cut(s) 1356
DdeI CTNAG 2 cut(s) 179, 870
DpnI GATC 6 cut(s) 177, 497, 637, 648, 664, 1191
DpnII GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
EaeI YGGCCR 2 cut(s) 1020, 1537
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
EciI GGCGGA 1 cut(s) 1184
Eco130I CCWWGG 1 cut(s) 704
Eco31I GGTCTC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 1216
Eco57I CTGAAG 4 cut(s) 605, 735, 1118, 1196
Eco88I CYCGRG 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 1216
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 1388
EcoT14I CCWWGG 1 cut(s) 704
EcoT22I ATGCAT 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 704
FaeI CATG 7 cut(s) 14, 70, 144, 580, 1204, 1331, 1437
FatI CATG 7 cut(s) 10, 66, 140, 576, 1200, 1327, 1433
FauI CCCGC 1 cut(s) 159
FauNDI CATATG 1 cut(s) 743
FbaI TGATCA 1 cut(s) 1189
Fnu4HI GCNGC 6 cut(s) 848, 919, 1232, 1431, 1455, 1475
FokI GGATG 2 cut(s) 477, 1130
Fsp4HI GCNGC 6 cut(s) 848, 919, 1232, 1431, 1455, 1475
FspBI CTAG 1 cut(s) 173
GluI GCNGC 6 cut(s) 848, 919, 1232, 1431, 1455, 1475
GsuI CTGGAG 2 cut(s) 1074, 1372
HaeIII GGCC 2 cut(s) 1022, 1539
HapII CCGG 1 cut(s) 379
HgaI GACGC 2 cut(s) 56, 992
Hin1II CATG 7 cut(s) 14, 70, 144, 580, 1204, 1331, 1437
HindIII AAGCTT 1 cut(s) 1130
HinfI GANTC 7 cut(s) 4, 153, 237, 719, 798, 1004, 1138
HpaII CCGG 1 cut(s) 379
HphI GGTGA 3 cut(s) 427, 950, 979
Hpy166II GTNNAC 2 cut(s) 125, 521
Hpy188I TCNGA 6 cut(s) 253, 781, 797, 1046, 1137, 1194
Hpy8I GTNNAC 2 cut(s) 125, 521
Hpy99I CGWCG 2 cut(s) 986, 1028
HpyAV CCTTC 4 cut(s) 104, 524, 629, 1474
HpyCH4III ACNGT 1 cut(s) 1186
HpyCH4IV ACGT 3 cut(s) 127, 961, 1394
HpyCH4V TGCA 7 cut(s) 144, 725, 859, 921, 1424, 1544, 1553
HpyF10VI GCNNNNNNNGC 8 cut(s) 706, 856, 918, 1129, 1240, 1306, 1430, 1550
HpyF3I CTNAG 2 cut(s) 179, 870
HpySE526I ACGT 3 cut(s) 127, 961, 1394
Hsp92II CATG 7 cut(s) 14, 70, 144, 580, 1204, 1331, 1437
Kpn2I TCCGGA 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 1189
Kzo9I GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
LmnI GCTCC 3 cut(s) 199, 381, 833
Lsp1109I GCAGC 5 cut(s) 905, 1218, 1441, 1442, 1486
LweI GCATC 3 cut(s) 153, 285, 1553
MaeI CTAG 1 cut(s) 173
MaeII ACGT 3 cut(s) 127, 961, 1394
MalI GATC 6 cut(s) 177, 497, 637, 648, 664, 1191
MboI GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
MflI RGATCY 3 cut(s) 175, 495, 646
MhlI GDGCHC 1 cut(s) 1494
MmeI TCCRAC 2 cut(s) 1032, 1054
Mph1103I ATGCAT 1 cut(s) 146
MroI TCCGGA 1 cut(s) 378
MroXI GAANNNNTTC 1 cut(s) 1531
MseI TTAA 5 cut(s) 72, 105, 477, 785, 932
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 1390
Mva1269I GAATGC 2 cut(s) 557, 830
MvaI CCWGG 1 cut(s) 1390
MwoI GCNNNNNNNGC 8 cut(s) 706, 856, 918, 1129, 1240, 1306, 1430, 1550
NdeI CATATG 1 cut(s) 743
NdeII GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
NlaIII CATG 7 cut(s) 14, 70, 144, 580, 1204, 1331, 1437
NlaIV GGNNCC 1 cut(s) 1218
NsiI ATGCAT 1 cut(s) 146
NspI RCATGY 3 cut(s) 14, 144, 1204
PaeR7I CTCGAG 1 cut(s) 148
PagI TCATGA 1 cut(s) 1327
PaqCI CACCTGC 1 cut(s) 525
PciI ACATGT 1 cut(s) 10
PctI GAATGC 2 cut(s) 557, 830
PdmI GAANNNNTTC 1 cut(s) 1531
PfeI GAWTC 7 cut(s) 4, 153, 237, 719, 798, 1004, 1138
PflFI GACNNNGTC 1 cut(s) 313
PfoI TCCNGGA 1 cut(s) 1388
PkrI GCNGC 6 cut(s) 849, 920, 1233, 1432, 1456, 1476
PpuMI RGGWCCY 1 cut(s) 1216
PscI ACATGT 1 cut(s) 10
PshAI GACNNNNGTC 1 cut(s) 13
Psp1406I AACGTT 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 1216
Psp6I CCWGG 1 cut(s) 1388
PspGI CCWGG 1 cut(s) 1388
PspN4I GGNNCC 1 cut(s) 1218
PspPI GGNCC 1 cut(s) 1216
PspPPI RGGWCCY 1 cut(s) 1216
PstI CTGCAG 1 cut(s) 727
PsuI RGATCY 3 cut(s) 175, 495, 646
PsyI GACNNNGTC 1 cut(s) 313
RsaI GTAC 1 cut(s) 1357
RsaNI GTAC 1 cut(s) 1356
SaqAI TTAA 5 cut(s) 72, 105, 477, 785, 932
SatI GCNGC 6 cut(s) 848, 919, 1232, 1431, 1455, 1475
Sau3AI GATC 6 cut(s) 175, 495, 635, 646, 662, 1189
Sau96I GGNCC 1 cut(s) 1216
ScrFI CCNGG 1 cut(s) 1390
SduI GDGCHC 1 cut(s) 1494
SfaNI GCATC 3 cut(s) 153, 285, 1553
SfcI CTRYAG 2 cut(s) 723, 734
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 1 cut(s) 1216
SlaI CTCGAG 1 cut(s) 148
SmlI CTYRAG 5 cut(s) 148, 338, 651, 803, 1159
SmoI CTYRAG 5 cut(s) 148, 338, 651, 803, 1159
SsiI CCGC 3 cut(s) 166, 848, 1169
SspI AATATT 1 cut(s) 449
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 1388
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 1 cut(s) 1186
TaiI ACGT 3 cut(s) 130, 964, 1397
TaqI TCGA 9 cut(s) 7, 149, 507, 661, 675, 754, 981, 1281, 1344
TaqII GACCGA 2 cut(s) 1131, 1347
TatI WGTACW 1 cut(s) 1355
TauI GCSGC 1 cut(s) 850
TfiI GAWTC 7 cut(s) 4, 153, 237, 719, 798, 1004, 1138
Tru1I TTAA 5 cut(s) 72, 105, 477, 785, 932
Tru9I TTAA 5 cut(s) 72, 105, 477, 785, 932
TscAI CASTG 1 cut(s) 187
TseI GCWGC 5 cut(s) 918, 1231, 1430, 1454, 1474
TspDTI ATGAA 6 cut(s) 55, 218, 584, 1008, 1017, 1362
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 1 cut(s) 187
Tth111I GACNNNGTC 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 1216
XapI RAATTY 5 cut(s) 62, 208, 301, 1349, 1531
XceI RCATGY 3 cut(s) 14, 144, 1204
XcmI CCANNNNNNNNNTGG 1 cut(s) 1015
XhoI CTCGAG 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 1531
XspI CTAG 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.