Rh2AG445400

Auxin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017046
Physical Location & Seq
Forward (+)
65792460 .. 65796161
3702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG445400.1

Sequence Viewer

Length: 930 bp
ATGGGTGTACCCTTGAAGATTTTAGCTGTCATATTGTCTACTTACGCACCTCCCTTTCAGTCTTCGCTTTCACTCTTCCCTTGTGCTGCTATAAGGACCACATTAATATCTGCAGAAAGGGGGTCTCATCATCAAGTAGTGGCTGTCCTCCTTTCATTAGTCCATTGTGTCCAGTGCATGGCTCGTTCTCAACTTGCTCCTAATCAATTTATGCGCAAGTTTTGTATATATATACCCAACCAAACCGATCGACTTGAGATGCCAAGCTCAGTAATCCTAAGCATGTTTGTGAAAATGGCTAATTACACCATTCTCTTCCTCTTTTCTCTCATCTTCTTATCCTCTTCTCGTGCTTCTGTCCAAGACTTCTGTGTTGCAGATCTAACTGGCCTTGATAGCCCTGCAGGATATTTCTGCAAGAACCCTGCTAAAGTCACTGTCAGTGACTTCGTGTATTCTGGCCTGGCCAAAGCTGGAAACACCACCAACATCATCCAAGCTGCTGTTACCCCCGCATTTGTCGCTCAATTTCCGGGTGTAAATGGTCTTGGCCTCTCTTTGGCAAGGCTTGACCTTGCGCCGGGGGGCGTTATCCCATTCCACACCCACCCTGGTGCTTCGGAAGTTCTTATTGTGTTGAGGGGTTATATCACTGCTGGCTTTGTTTCTTCAGCCAACTCTGTTTACGTGAAGACTCTTAAAAAGGGAGATGTCATGGTTTTCCCACAAGGGCTGTTGCACTTTCAAGTAAATGCTGCTAAAATACATGGCAAGGCCATTGTAAGCTTTAGCAGTGCCAGTCCAGGTCTCCAAATCCTCGATTTCGCACTTTTCGCTAACGATTTACCTTCTTCATTGGTGGGGAAGACAACTTTCCTTGATCCTGCTCAAATCAAGAAACTCAAGGGAGTCCTTGGTGGCACTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

309

Amino Acids

32.86

Weight (kDa)

9.43

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 154 - 298 1.5e-36 Cupin
Cupin_2 PF07883 190 - 252 5.7e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017046)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72610
fragaria_vesca FvH4_6g34740
malus_domestica MD09G1183200.v1.1
prunus_persica Prupe.3G041700_v2.0.a1
pyrus_communis pycom09g10090
rosa_chinensis RchiOBHm_Chr2g0145401
rosa_laevigata RLG00000020142
rosa_multiflora Rmu_sc0001298.1_g000057
rosa_rugosa Rorug02G0392000
rosa_samantha Rh2AG445400 Rh2BG456200 Rh2DG466100
rosa_wichuraiana Rw2G036360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 215
AccB7I CCANNNNNTGG 1 cut(s) 178
AccI GTMKAC 1 cut(s) 38
AciI CCGC 1 cut(s) 513
AclWI GGATC 1 cut(s) 875
AcoI YGGCCR 1 cut(s) 465
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 1 cut(s) 9
AfiI CCNNNNNNNGG 3 cut(s) 178, 559, 580
AgsI TTSAA 2 cut(s) 16, 746
AjnI CCWGG 3 cut(s) 462, 610, 802
AleI CACNNNNGTG 1 cut(s) 612
AluBI AGCT 5 cut(s) 26, 267, 473, 500, 786
AluI AGCT 5 cut(s) 26, 267, 473, 500, 786
Alw26I GTCTC 2 cut(s) 129, 812
AlwI GGATC 1 cut(s) 875
AoxI GGCC 5 cut(s) 388, 460, 465, 550, 774
ApeKI GCWGC 3 cut(s) 86, 500, 755
AseI ATTAAT 1 cut(s) 104
AspLEI GCGC 2 cut(s) 216, 580
AspS9I GGNCC 1 cut(s) 96
AsuC2I CCSGG 2 cut(s) 534, 582
AvaII GGWCC 1 cut(s) 96
BalI TGGCCA 1 cut(s) 467
BauI CACGAG 1 cut(s) 348
BbsI GAAGAC 3 cut(s) 54, 698, 872
BbvI GCAGC 3 cut(s) 73, 487, 742
BciT130I CCWGG 3 cut(s) 464, 612, 804
BcnI CCSGG 2 cut(s) 534, 582
BcoDI GTCTC 2 cut(s) 129, 812
BfmI CTRYAG 2 cut(s) 111, 402
BglII AGATCT 1 cut(s) 379
BisI GCNGC 3 cut(s) 87, 501, 756
BlsI GCNGC 3 cut(s) 88, 502, 757
Bme1390I CCNGG 5 cut(s) 464, 534, 582, 612, 804
Bme18I GGWCC 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 96
BmrFI CCNGG 5 cut(s) 464, 534, 582, 612, 804
BmsI GCATC 1 cut(s) 249
BpiI GAAGAC 3 cut(s) 54, 698, 872
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 2 cut(s) 275, 887
BpuMI CCSGG 2 cut(s) 534, 582
BsaAI YACGTR 1 cut(s) 688
BsaI GGTCTC 2 cut(s) 129, 812
BsaJI CCNNGG 3 cut(s) 581, 610, 913
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 559, 580
Bse1I ACTGG 4 cut(s) 172, 391, 798, 928
BseBI CCWGG 3 cut(s) 464, 612, 804
BseDI CCNNGG 3 cut(s) 581, 610, 913
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 3 cut(s) 178, 559, 580
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 4 cut(s) 172, 391, 798, 928
BseXI GCAGC 3 cut(s) 73, 487, 742
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 5 cut(s) 390, 462, 467, 552, 776
BsiEI CGRYCG 1 cut(s) 250
BsiSI CCGG 2 cut(s) 533, 581
BslI CCNNNNNNNGG 3 cut(s) 178, 559, 580
BsmAI GTCTC 2 cut(s) 129, 812
BsnI GGCC 5 cut(s) 390, 462, 467, 552, 776
Bso31I GGTCTC 2 cut(s) 129, 812
Bsp143I GATC 3 cut(s) 247, 379, 880
BspACI CCGC 1 cut(s) 513
BspANI GGCC 5 cut(s) 390, 462, 467, 552, 776
BspCNI CTCAG 1 cut(s) 281
BspMAI CTGCAG 2 cut(s) 115, 406
BspPI GGATC 1 cut(s) 875
BspTNI GGTCTC 2 cut(s) 129, 812
BsrI ACTGG 4 cut(s) 172, 391, 798, 928
BssECI CCNNGG 3 cut(s) 581, 610, 913
BssMI GATC 3 cut(s) 247, 379, 880
BssSI CACGAG 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 913
Bst2BI CACGAG 1 cut(s) 348
Bst2UI CCWGG 3 cut(s) 464, 612, 804
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 3 cut(s) 80, 320, 349
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 1 cut(s) 658
BstDEI CTNAG 2 cut(s) 268, 278
BstF5I GGATG 1 cut(s) 492
BstHHI GCGC 2 cut(s) 216, 580
BstKTI GATC 3 cut(s) 250, 382, 883
BstMAI GTCTC 2 cut(s) 129, 812
BstMBI GATC 3 cut(s) 247, 379, 880
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 3 cut(s) 396, 521, 833
BstNI CCWGG 3 cut(s) 464, 612, 804
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 5 cut(s) 462, 532, 580, 610, 802
BstSFI CTRYAG 2 cut(s) 111, 402
BstV1I GCAGC 3 cut(s) 73, 487, 742
BstV2I GAAGAC 3 cut(s) 54, 698, 872
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 5 cut(s) 390, 462, 467, 552, 776
BtsCI GGATG 1 cut(s) 492
BtsI GCAGTG 2 cut(s) 651, 799
BtsIMutI CAGTG 6 cut(s) 179, 435, 448, 651, 799, 921
Cac8I GCNNGC 1 cut(s) 658
CfoI GCGC 2 cut(s) 216, 580
Cfr13I GGNCC 1 cut(s) 96
Csp6I GTAC 1 cut(s) 8
CviAII CATG 4 cut(s) 178, 283, 715, 767
CviQI GTAC 1 cut(s) 8
DdeI CTNAG 2 cut(s) 268, 278
DpnI GATC 3 cut(s) 249, 381, 882
DpnII GATC 3 cut(s) 247, 379, 880
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 3 cut(s) 80, 320, 349
EarI CTCTTC 3 cut(s) 80, 320, 349
Eco130I CCWWGG 1 cut(s) 913
Eco31I GGTCTC 2 cut(s) 129, 812
Eco47I GGWCC 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 654
EcoRII CCWGG 3 cut(s) 462, 610, 802
EcoT14I CCWWGG 1 cut(s) 913
ErhI CCWWGG 1 cut(s) 913
FaeI CATG 4 cut(s) 181, 286, 718, 770
FatI CATG 4 cut(s) 177, 282, 714, 766
FauI CCCGC 1 cut(s) 520
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 3 cut(s) 87, 501, 756
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 3 cut(s) 87, 501, 756
FspI TGCGCA 1 cut(s) 215
GlaI GCGC 2 cut(s) 215, 579
GluI GCNGC 3 cut(s) 87, 501, 756
HaeIII GGCC 5 cut(s) 390, 462, 467, 552, 776
HapII CCGG 2 cut(s) 533, 581
HhaI GCGC 2 cut(s) 216, 580
Hin1II CATG 4 cut(s) 181, 286, 718, 770
Hin6I GCGC 2 cut(s) 214, 578
HinP1I GCGC 2 cut(s) 214, 578
HindIII AAGCTT 1 cut(s) 784
HinfI GANTC 2 cut(s) 694, 909
HpaII CCGG 2 cut(s) 533, 581
Hpy166II GTNNAC 3 cut(s) 8, 39, 685
Hpy188I TCNGA 1 cut(s) 622
Hpy188III TCNNGA 1 cut(s) 895
Hpy8I GTNNAC 3 cut(s) 8, 39, 685
HpyAV CCTTC 1 cut(s) 858
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 6 cut(s) 113, 177, 377, 404, 417, 739
HpyF10VI GCNNNNNNNGC 3 cut(s) 396, 521, 833
HpyF3I CTNAG 2 cut(s) 268, 278
HpySE526I ACGT 1 cut(s) 687
Hsp92II CATG 4 cut(s) 181, 286, 718, 770
HspAI GCGC 2 cut(s) 214, 578
Kzo9I GATC 3 cut(s) 247, 379, 880
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 3 cut(s) 73, 487, 742
LweI GCATC 1 cut(s) 249
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 3 cut(s) 433, 443, 505
MalI GATC 3 cut(s) 249, 381, 882
MboI GATC 3 cut(s) 247, 379, 880
MflI RGATCY 1 cut(s) 379
MlsI TGGCCA 1 cut(s) 467
MluCI AATT 3 cut(s) 206, 301, 527
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 2 cut(s) 688, 918
MnlI CCTC 7 cut(s) 60, 158, 329, 352, 563, 633, 827
Mox20I TGGCCA 1 cut(s) 467
MscI TGGCCA 1 cut(s) 467
MseI TTAA 2 cut(s) 104, 699
MslI CAYNNNNRTG 2 cut(s) 287, 612
Msp20I TGGCCA 1 cut(s) 467
MspI CCGG 2 cut(s) 533, 581
MspR9I CCNGG 5 cut(s) 464, 534, 582, 612, 804
MvaI CCWGG 3 cut(s) 464, 612, 804
MwoI GCNNNNNNNGC 3 cut(s) 396, 521, 833
NciI CCSGG 2 cut(s) 534, 582
NdeII GATC 3 cut(s) 247, 379, 880
NlaIII CATG 4 cut(s) 181, 286, 718, 770
NmuCI GTSAC 2 cut(s) 433, 443
NsbI TGCGCA 1 cut(s) 215
NspI RCATGY 1 cut(s) 286
OliI CACNNNNGTG 1 cut(s) 612
PflMI CCANNNNNTGG 1 cut(s) 178
PkrI GCNGC 3 cut(s) 88, 502, 757
Ple19I CGATCG 1 cut(s) 250
PleI GAGTC 2 cut(s) 688, 917
PpsI GAGTC 2 cut(s) 688, 917
Ppu21I YACGTR 1 cut(s) 688
PshBI ATTAAT 1 cut(s) 104
Psp6I CCWGG 3 cut(s) 462, 610, 802
PspGI CCWGG 3 cut(s) 462, 610, 802
PspPI GGNCC 1 cut(s) 96
PstI CTGCAG 2 cut(s) 115, 406
PsuI RGATCY 1 cut(s) 379
PvuI CGATCG 1 cut(s) 250
RsaI GTAC 1 cut(s) 9
RsaNI GTAC 1 cut(s) 8
RseI CAYNNNNRTG 2 cut(s) 287, 612
SaqAI TTAA 2 cut(s) 104, 699
SatI GCNGC 3 cut(s) 87, 501, 756
Sau3AI GATC 3 cut(s) 247, 379, 880
Sau96I GGNCC 1 cut(s) 96
SbfI CCTGCAGG 1 cut(s) 406
SchI GAGTC 2 cut(s) 688, 918
ScrFI CCNGG 5 cut(s) 464, 534, 582, 612, 804
SdaI CCTGCAGG 1 cut(s) 406
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 2 cut(s) 111, 402
SinI GGWCC 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 287, 612
SmlI CTYRAG 2 cut(s) 254, 902
SmoI CTYRAG 2 cut(s) 254, 902
Sse8387I CCTGCAGG 1 cut(s) 406
Sse9I AATT 3 cut(s) 206, 301, 527
SsiI CCGC 1 cut(s) 513
StyD4I CCNGG 5 cut(s) 462, 532, 580, 610, 802
StyI CCWWGG 1 cut(s) 913
TaaI ACNGT 1 cut(s) 439
TaiI ACGT 1 cut(s) 690
TaqI TCGA 2 cut(s) 250, 819
TasI AATT 3 cut(s) 206, 301, 527
Tru1I TTAA 2 cut(s) 104, 699
Tru9I TTAA 2 cut(s) 104, 699
TscAI CASTG 6 cut(s) 179, 442, 448, 658, 799, 928
TseFI GTSAC 2 cut(s) 433, 443
TseI GCWGC 3 cut(s) 86, 500, 755
Tsp45I GTSAC 2 cut(s) 433, 443
TspDTI ATGAA 2 cut(s) 144, 843
TspRI CASTG 6 cut(s) 179, 442, 448, 658, 799, 928
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 96
VspI ATTAAT 1 cut(s) 104
XceI RCATGY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmiI GTMKAC 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.