Rh2BG065500

Rit1 N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003887
Physical Location & Seq
Reverse (-)
5002585 .. 5006250
3666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG065500.1

Sequence Viewer

Length: 1383 bp
ATGGAAGACGACGAGGATACGCGTCAGCTGAGCATATACAGAGCCGCCAGAACCATAAAGCGCAGGGACAACACCCTCTACAACGCCCTCAGATCCATCTACGACGACTCTGTCTTCGTCGGCGAGATCTTCCAGCTCTGGCCCGACCTCCCTCTCCTCGCCAACCTCCGATGTGGCCTCTGGTACTCCCGAACGTTCCACGCCACGTGTTACTTCAAGTCCACCGACGGCCACAACAACAACTGGTCCTTCAACACGTCCCGGCTCAACCTCCACGTCGCTCAGCTCGCAGGACAGAAGGGCGGGTGTTTTATTGTGGATGCAACTAGGAGAGGGAAGCGGTTTCCGGACAGCATGTCGAAGACGATTCCGATTTGGACTTGCGTTTTGAACCGTTCGATTCAAAACCATAGAGAAAGAATGCGGCAGAGTGCTCTGGTTAATGATGGGCTGAGTTTAGAAAACCACAAGGAGTCAAGTACTTGTGATGAATATTATGGAGATGCAAGACAGATGCCTATTGATTGGGATTGTTCCTTGCATCTTCCGCTTTGGGTGTCTGGAACAGAGAGAGCTTCTATTGAGAAGCGATTAGAAGAATGGACTCAACAGTTGGATGCAAGTGGAGCTGATATTAGCTCTCTTGCTTCTTGTTTAAAAAAGCCTTTACGCCCTCTATGGATATCACAGAAAACTGTCATTTGGTTGAATGAAGTTCCAGATCATGAGTCCTGGGATTTCACACCTATCATTCTTGTTTCTGCCTCATCCCCGAGCGGTGTTGTGCAACGCAGGATTAGTTCAGAATTTAGCTGGAATTATATACCAGGAGCTGGTGATGATGAAGAAAGTTGGGCGAGGGGTTTAACACCTAGTCTTTTCTGGAATTGTGCCTATGATATTATAAATGCAGGTCCTGAAATGTGTAATCAGATGGTGGCTGATATAGTTGAGAAGGATAGAGTGTATCGTGCTCGAAGGGGACAGGATGCTCCTCAGGTCACTGTTAAGGCTCCAAATCTGTTACCAAGCTCGGATCATCGGTTGAGGGACAATAATGAGTCACAATTAGCTGTGGATCTTGCAAACTTTAATATCGATCGTAATTCTTCTGATGAAGATTGTGTTATATCTTGGCTTGGTTTGACTAACCTTGCTGTAGGCACATCTCAGAACGGTATGCTGCCTTATGAACTCATCTTGTTTGTAAGCATCCAAAATCAAACAACACAGATTCATACAACTAAACCCTTCACTTCAGACATTCTTGTCATATGTTCATATATCAACTCCTTCACTCTTCTGTTTCTAATTTCCATCCAGCTTTATCCAAGTATCTTTCCATTCACTTTTCTTTTTTTAGTTCTACATGCATCTTACTGA

Protein Analysis

460

Amino Acids

52.47

Weight (kDa)

5.53

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rit1_C PF17184 19 - 318 3.1e-96 Rit1 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AasI GACNNNNNNGTC 2 cut(s) 110, 1268
Acc36I ACCTGC 1 cut(s) 902
AccB7I CCANNNNNTGG 1 cut(s) 833
AccBSI CCGCTC 1 cut(s) 777
AccII CGCG 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 6 cut(s) 45, 303, 340, 424, 548, 777
AclI AACGTT 1 cut(s) 194
AclWI GGATC 3 cut(s) 87, 1044, 1086
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 1 cut(s) 806
AcuI CTGAAG 1 cut(s) 1242
AcvI CACGTG 1 cut(s) 207
AfaI GTAC 2 cut(s) 185, 481
AfiI CCNNNNNNNGG 1 cut(s) 833
AflIII ACRYGT 3 cut(s) 20, 206, 255
AgsI TTSAA 5 cut(s) 217, 253, 391, 404, 709
AhdI GACNNNNNGTC 1 cut(s) 355
AjiI CACGTC 2 cut(s) 258, 277
AjnI CCWGG 2 cut(s) 731, 826
Alw21I GWGCWC 2 cut(s) 436, 976
AlwI GGATC 3 cut(s) 87, 1044, 1086
AlwNI CAGNNNCTG 2 cut(s) 833, 917
Ama87I CYCGRG 1 cut(s) 772
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 3 cut(s) 140, 175, 229
ApeKI GCWGC 1 cut(s) 1183
ApoI RAATTY 1 cut(s) 806
ArsI GACNNNNNNTTYG 4 cut(s) 98, 130, 370, 402
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 3 cut(s) 141, 246, 914
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 848
AvaI CYCGRG 1 cut(s) 772
AvaII GGWCC 2 cut(s) 246, 914
AxyI CCTNAGG 1 cut(s) 996
BbrPI CACGTG 1 cut(s) 207
BbsI GAAGAC 3 cut(s) 12, 106, 368
Bbv12I GWGCWC 2 cut(s) 436, 976
BbvI GCAGC 1 cut(s) 1170
BccI CCATC 4 cut(s) 104, 440, 928, 1325
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 2 cut(s) 733, 828
BciVI GTATCC 1 cut(s) 10
BcnI CCSGG 1 cut(s) 262
BfaI CTAG 2 cut(s) 327, 873
BfmI CTRYAG 1 cut(s) 1158
BfuAI ACCTGC 1 cut(s) 902
BfuI GTATCC 1 cut(s) 10
BglII AGATCT 1 cut(s) 126
BisI GCNGC 3 cut(s) 45, 425, 1184
BlpI GCTNAGC 2 cut(s) 29, 282
BlsI GCNGC 3 cut(s) 46, 426, 1185
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 3 cut(s) 262, 733, 828
Bme18I GGWCC 2 cut(s) 246, 914
BmeRI GACNNNNNGTC 1 cut(s) 355
BmeT110I CYCGRG 1 cut(s) 772
BmgBI CACGTC 2 cut(s) 258, 277
BmgT120I GGNCC 3 cut(s) 141, 246, 914
BmiI GGNNCC 1 cut(s) 1014
BmrFI CCNGG 3 cut(s) 262, 733, 828
BmsI GCATC 7 cut(s) 310, 493, 504, 550, 607, 979, 1221
BpiI GAAGAC 3 cut(s) 12, 106, 368
Bpu1102I GCTNAGC 2 cut(s) 29, 282
BpuMI CCSGG 1 cut(s) 262
Bsa29I ATCGAT 1 cut(s) 1098
BsaAI YACGTR 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 732
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 1 cut(s) 833
Bse1I ACTGG 1 cut(s) 248
Bse21I CCTNAGG 1 cut(s) 996
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 2 cut(s) 733, 828
BseCI ATCGAT 1 cut(s) 1098
BseDI CCNNGG 1 cut(s) 732
BseGI GGATG 6 cut(s) 325, 622, 767, 994, 1212, 1317
BseLI CCNNNNNNNGG 1 cut(s) 833
BseMII CTCAG 6 cut(s) 20, 103, 296, 443, 1010, 1184
BseNI ACTGG 1 cut(s) 248
BseRI GAGGAG 2 cut(s) 146, 984
BseXI GCAGC 1 cut(s) 1170
Bsh1236I CGCG 1 cut(s) 22
Bsh1285I CGRYCG 1 cut(s) 1102
BshFI GGCC 3 cut(s) 142, 177, 231
BshVI ATCGAT 1 cut(s) 1098
BsiEI CGRYCG 1 cut(s) 1102
BsiHKAI GWGCWC 2 cut(s) 436, 976
BsiHKCI CYCGRG 1 cut(s) 772
BsiSI CCGG 2 cut(s) 262, 347
BslFI GGGAC 4 cut(s) 80, 244, 996, 1064
BslI CCNNNNNNNGG 1 cut(s) 833
BsmFI GGGAC 4 cut(s) 80, 244, 996, 1064
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 3 cut(s) 142, 177, 231
BsoBI CYCGRG 1 cut(s) 772
Bsp1286I GDGCHC 2 cut(s) 436, 976
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
Bsp1720I GCTNAGC 2 cut(s) 29, 282
BspACI CCGC 6 cut(s) 45, 303, 340, 424, 548, 777
BspANI GGCC 3 cut(s) 142, 177, 231
BspCNI CTCAG 6 cut(s) 21, 102, 295, 444, 1009, 1183
BspDI ATCGAT 1 cut(s) 1098
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 1 cut(s) 22
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 1 cut(s) 1014
BspMI ACCTGC 1 cut(s) 902
BspPI GGATC 3 cut(s) 87, 1044, 1086
BsrBI CCGCTC 1 cut(s) 777
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 732
BssMI GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
Bst2UI CCWGG 2 cut(s) 733, 828
Bst4CI ACNGT 5 cut(s) 395, 612, 697, 1006, 1178
Bst6I CTCTTC 1 cut(s) 1305
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 6 cut(s) 29, 89, 282, 452, 996, 1170
BstF5I GGATG 6 cut(s) 325, 622, 767, 994, 1212, 1317
BstFNI CGCG 1 cut(s) 22
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 6 cut(s) 95, 129, 724, 1039, 1081, 1102
BstMBI GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
BstMCI CGRYCG 1 cut(s) 1102
BstMWI GCNNNNNNNGC 3 cut(s) 287, 547, 626
BstNI CCWGG 2 cut(s) 733, 828
BstNSI RCATGY 2 cut(s) 358, 1373
BstSCI CCNGG 3 cut(s) 260, 731, 826
BstSFI CTRYAG 1 cut(s) 1158
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 1170
BstV2I GAAGAC 3 cut(s) 12, 106, 368
BstX2I RGATCY 3 cut(s) 92, 126, 1078
BstYI RGATCY 3 cut(s) 92, 126, 1078
Bsu15I ATCGAT 1 cut(s) 1098
Bsu36I CCTNAGG 1 cut(s) 996
BsuI GTATCC 1 cut(s) 10
BsuRI GGCC 3 cut(s) 142, 177, 231
BsuTUI ATCGAT 1 cut(s) 1098
BtrI CACGTC 2 cut(s) 258, 277
BtsCI GGATG 6 cut(s) 325, 622, 767, 994, 1212, 1317
BtsIMutI CAGTG 1 cut(s) 1002
BveI ACCTGC 1 cut(s) 902
Cac8I GCNNGC 1 cut(s) 288
CaiI CAGNNNCTG 2 cut(s) 833, 917
CciI TCATGA 1 cut(s) 724
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 141, 246, 914
ClaI ATCGAT 1 cut(s) 1098
CseI GACGC 1 cut(s) 11
Csp6I GTAC 2 cut(s) 184, 480
CviAII CATG 3 cut(s) 355, 725, 1370
CviQI GTAC 2 cut(s) 184, 480
DdeI CTNAG 6 cut(s) 29, 89, 282, 452, 996, 1170
DpnI GATC 6 cut(s) 94, 128, 723, 1038, 1080, 1101
DpnII GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
DraI TTTAAA 1 cut(s) 657
DrdI GACNNNNNNGTC 2 cut(s) 110, 1268
DriI GACNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 2 cut(s) 110, 1268
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 1305
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 1305
Eco32I GATATC 1 cut(s) 684
Eco47I GGWCC 2 cut(s) 246, 914
Eco57I CTGAAG 1 cut(s) 1242
Eco72I CACGTG 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 996
Eco88I CYCGRG 1 cut(s) 772
EcoO109I RGGNCCY 1 cut(s) 914
EcoRII CCWGG 2 cut(s) 731, 826
EcoRV GATATC 1 cut(s) 684
EcoT22I ATGCAT 1 cut(s) 1375
FaeI CATG 3 cut(s) 358, 728, 1373
FaqI GGGAC 4 cut(s) 80, 244, 996, 1064
FatI CATG 3 cut(s) 354, 724, 1369
FauI CCCGC 1 cut(s) 296
FauNDI CATATG 1 cut(s) 1274
Fnu4HI GCNGC 3 cut(s) 45, 425, 1184
FokI GGATG 6 cut(s) 332, 629, 754, 1001, 1199, 1304
Fsp4HI GCNGC 3 cut(s) 45, 425, 1184
FspBI CTAG 2 cut(s) 327, 873
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 45, 425, 1184
HaeIII GGCC 3 cut(s) 142, 177, 231
HapII CCGG 2 cut(s) 262, 347
HgaI GACGC 1 cut(s) 11
HhaI GCGC 1 cut(s) 63
Hin1II CATG 3 cut(s) 358, 728, 1373
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HinfI GANTC 8 cut(s) 107, 367, 400, 473, 604, 728, 1061, 1234
HpaII CCGG 2 cut(s) 262, 347
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 9 cut(s) 92, 170, 372, 805, 933, 1036, 1114, 1173, 1261
Hpy188III TCNNGA 7 cut(s) 189, 347, 561, 719, 725, 883, 917
Hpy8I GTNNAC 1 cut(s) 222
Hpy99I CGWCG 5 cut(s) 14, 107, 122, 230, 281
HpyAV CCTTC 6 cut(s) 259, 292, 949, 972, 1261, 1303
HpyCH4III ACNGT 5 cut(s) 395, 612, 697, 1006, 1178
HpyCH4IV ACGT 4 cut(s) 194, 206, 257, 276
HpyCH4V TGCA 8 cut(s) 323, 506, 541, 620, 787, 911, 1085, 1373
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 547, 626
HpyF3I CTNAG 6 cut(s) 29, 89, 282, 452, 996, 1170
HpySE526I ACGT 4 cut(s) 194, 206, 257, 276
Hsp92II CATG 3 cut(s) 358, 728, 1373
HspAI GCGC 1 cut(s) 61
Kpn2I TCCGGA 1 cut(s) 346
Kzo9I GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
LmnI GCTCC 4 cut(s) 626, 830, 997, 1018
Lsp1109I GCAGC 1 cut(s) 1170
LweI GCATC 7 cut(s) 310, 493, 504, 550, 607, 979, 1221
MaeI CTAG 2 cut(s) 327, 873
MaeII ACGT 4 cut(s) 194, 206, 257, 276
MaeIII GTNAC 4 cut(s) 209, 1000, 1023, 1062
MalI GATC 6 cut(s) 94, 128, 723, 1038, 1080, 1101
MbiI CCGCTC 1 cut(s) 777
MboI GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
MflI RGATCY 3 cut(s) 92, 126, 1078
MhlI GDGCHC 2 cut(s) 436, 976
MluCI AATT 6 cut(s) 806, 817, 886, 1067, 1105, 1311
MluI ACGCGT 1 cut(s) 20
MlyI GAGTC 5 cut(s) 101, 482, 598, 737, 1070
MmeI TCCRAC 1 cut(s) 594
Mph1103I ATGCAT 1 cut(s) 1375
MroI TCCGGA 1 cut(s) 346
MseI TTAA 5 cut(s) 441, 656, 866, 1008, 1092
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 2 cut(s) 262, 347
MspR9I CCNGG 3 cut(s) 262, 733, 828
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 2 cut(s) 733, 828
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 3 cut(s) 287, 547, 626
NciI CCSGG 1 cut(s) 262
NdeI CATATG 1 cut(s) 1274
NdeII GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
NlaIII CATG 3 cut(s) 358, 728, 1373
NlaIV GGNNCC 1 cut(s) 1014
NmuCI GTSAC 2 cut(s) 1000, 1062
NsiI ATGCAT 1 cut(s) 1375
NspI RCATGY 2 cut(s) 358, 1373
PagI TCATGA 1 cut(s) 724
PctI GAATGC 1 cut(s) 426
PfeI GAWTC 3 cut(s) 367, 400, 1234
PflFI GACNNNGTC 1 cut(s) 110
PflMI CCANNNNNTGG 1 cut(s) 833
PkrI GCNGC 3 cut(s) 46, 426, 1185
Ple19I CGATCG 1 cut(s) 1102
PleI GAGTC 5 cut(s) 101, 481, 598, 736, 1069
PmaCI CACGTG 1 cut(s) 207
PmlI CACGTG 1 cut(s) 207
PpsI GAGTC 5 cut(s) 101, 481, 598, 736, 1069
Ppu21I YACGTR 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 914
PsiI TTATAA 1 cut(s) 905
Psp1406I AACGTT 1 cut(s) 194
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 2 cut(s) 731, 826
PspCI CACGTG 1 cut(s) 207
PspGI CCWGG 2 cut(s) 731, 826
PspN4I GGNNCC 1 cut(s) 1014
PspPI GGNCC 3 cut(s) 141, 246, 914
PspPPI RGGWCCY 1 cut(s) 914
PstNI CAGNNNCTG 2 cut(s) 833, 917
PsuI RGATCY 3 cut(s) 92, 126, 1078
PsyI GACNNNGTC 1 cut(s) 110
PvuI CGATCG 1 cut(s) 1102
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 2 cut(s) 185, 481
RsaNI GTAC 2 cut(s) 184, 480
SaqAI TTAA 5 cut(s) 441, 656, 866, 1008, 1092
SatI GCNGC 3 cut(s) 45, 425, 1184
Sau3AI GATC 6 cut(s) 92, 126, 721, 1036, 1078, 1099
Sau96I GGNCC 3 cut(s) 141, 246, 914
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 5 cut(s) 101, 482, 598, 737, 1070
ScrFI CCNGG 3 cut(s) 262, 733, 828
SduI GDGCHC 2 cut(s) 436, 976
SfaNI GCATC 7 cut(s) 310, 493, 504, 550, 607, 979, 1221
SfcI CTRYAG 1 cut(s) 1158
SinI GGWCC 2 cut(s) 246, 914
Sse9I AATT 6 cut(s) 806, 817, 886, 1067, 1105, 1311
SsiI CCGC 6 cut(s) 45, 303, 340, 424, 548, 777
SspI AATATT 1 cut(s) 494
SspMI CTAG 2 cut(s) 327, 873
StyD4I CCNGG 3 cut(s) 260, 731, 826
TaaI ACNGT 5 cut(s) 395, 612, 697, 1006, 1178
TaiI ACGT 4 cut(s) 197, 209, 260, 279
TaqI TCGA 4 cut(s) 359, 398, 976, 1098
TasI AATT 6 cut(s) 806, 817, 886, 1067, 1105, 1311
TatI WGTACW 1 cut(s) 479
TauI GCSGC 2 cut(s) 47, 427
TfiI GAWTC 3 cut(s) 367, 400, 1234
Tru1I TTAA 5 cut(s) 441, 656, 866, 1008, 1092
Tru9I TTAA 5 cut(s) 441, 656, 866, 1008, 1092
TscAI CASTG 1 cut(s) 1009
TseFI GTSAC 2 cut(s) 1000, 1062
TseI GCWGC 1 cut(s) 1183
Tsp45I GTSAC 2 cut(s) 1000, 1062
TspDTI ATGAA 7 cut(s) 504, 726, 858, 1131, 1206, 1226, 1269
TspRI CASTG 1 cut(s) 1009
Tth111I GACNNNGTC 1 cut(s) 110
Van91I CCANNNNNTGG 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 246, 914
XapI RAATTY 1 cut(s) 806
XceI RCATGY 2 cut(s) 358, 1373
XspI CTAG 2 cut(s) 327, 873
ZrmI AGTACT 1 cut(s) 481
Zsp2I ATGCAT 1 cut(s) 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.