Rh2BG143500

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015775
Physical Location & Seq
Reverse (-)
12388868 .. 12389520
653 bp
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UTR
Exon/CDS
Intron
Rh2BG143500.1

Sequence Viewer

Length: 417 bp
ATGGCTCCTCCCAGTCATCTCGGTCCTCCCGAGCTCCACAAACCCTCACCCACCACCACCTCTCCGCCGCCACCCGAACCCGAATCCAAACCTCAAGGCCTGGAACTGGAAAGTCAGACAAGGAAGGCTTCCATATGGCGGCGTTTTGGCTCCACAAGCACCAGCCCAAAACTCGCTTGCAACCTCGCGTCCTTCTTCGAGTTCGGTTACTTCAAGAACCTGCCGGAGATAGTGTACCGGCTTCTAGAGGGCGAGGACGTGAGGAAGAAACAGAAGGCGGAGTGGCGGGAGAGGAAATGCACCGGAGGCAGGAAGCGACGGGAATCCGGATCCGACGGCCAAACCGGGTCGAGCTCCGAGAAGAAGAGAATCAGAAGAAAGAGAGGAAAATGGAGAGAGAAGAAGACGATTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

138

Amino Acids

15.78

Weight (kDa)

10.28

Isoelectric Point (pI)

72.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 62 - 128 7.9e-06 Domain of unknown function (DUF2828)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 228
AccII CGCG 1 cut(s) 188
AccIII TCCGGA 1 cut(s) 326
AciI CCGC 5 cut(s) 65, 68, 139, 278, 286
AclWI GGATC 2 cut(s) 324, 337
AcoI YGGCCR 1 cut(s) 337
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 3 cut(s) 106, 138, 309
AgsI TTSAA 1 cut(s) 214
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 2 cut(s) 34, 354
AluI AGCT 2 cut(s) 34, 354
Alw21I GWGCWC 2 cut(s) 36, 356
AlwI GGATC 2 cut(s) 324, 337
Ama87I CYCGRG 1 cut(s) 29
Aor13HI TCCGGA 1 cut(s) 326
AoxI GGCC 2 cut(s) 97, 337
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 23
BamHI GGATCC 1 cut(s) 329
BanII GRGCYC 2 cut(s) 36, 356
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 2 cut(s) 36, 356
BceAI ACGGC 1 cut(s) 352
BciT130I CCWGG 1 cut(s) 101
BcnI CCSGG 1 cut(s) 346
BfaI CTAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 228
BisI GCNGC 2 cut(s) 68, 140
BlsI GCNGC 2 cut(s) 69, 141
Bme1390I CCNGG 2 cut(s) 101, 346
Bme18I GGWCC 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 3 cut(s) 6, 151, 331
BmrFI CCNGG 2 cut(s) 101, 346
BmrI ACTGGG 1 cut(s) 6
BmuI ACTGGG 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 346
BsaWI WCCGGW 2 cut(s) 302, 326
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 138, 309
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 2 cut(s) 12, 111
BseAI TCCGGA 1 cut(s) 326
BseBI CCWGG 1 cut(s) 101
BseLI CCNNNNNNNGG 3 cut(s) 106, 138, 309
BseNI ACTGG 2 cut(s) 12, 111
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 2 cut(s) 99, 339
BsiHKAI GWGCWC 2 cut(s) 36, 356
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 5 cut(s) 224, 238, 303, 327, 345
BslI CCNNNNNNNGG 3 cut(s) 106, 138, 309
BsnI GGCC 2 cut(s) 99, 339
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 36, 356
Bsp13I TCCGGA 1 cut(s) 326
Bsp143I GATC 1 cut(s) 329
BspACI CCGC 5 cut(s) 65, 68, 139, 278, 286
BspANI GGCC 2 cut(s) 99, 339
BspEI TCCGGA 1 cut(s) 326
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 3 cut(s) 6, 151, 331
BspMI ACCTGC 1 cut(s) 228
BspPI GGATC 2 cut(s) 324, 337
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 2 cut(s) 12, 111
BssAI RCCGGY 1 cut(s) 237
BssMI GATC 1 cut(s) 329
Bst2UI CCWGG 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 178
BstFNI CGCG 1 cut(s) 188
BstKTI GATC 1 cut(s) 332
BstMBI GATC 1 cut(s) 329
BstMWI GCNNNNNNNGC 2 cut(s) 156, 306
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 99, 344
BstUI CGCG 1 cut(s) 188
BstV2I GAAGAC 1 cut(s) 410
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BsuRI GGCC 2 cut(s) 99, 339
BtrI CACGTC 1 cut(s) 259
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 23
CseI GACGC 1 cut(s) 177
Csp6I GTAC 1 cut(s) 235
CviJI RGCY 9 cut(s) 5, 34, 99, 128, 150, 165, 241, 339, 354
CviKI_1 RGCY 9 cut(s) 5, 34, 99, 128, 150, 165, 241, 339, 354
CviQI GTAC 1 cut(s) 235
DpnI GATC 1 cut(s) 331
DpnII GATC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
EciI GGCGGA 2 cut(s) 54, 293
Ecl136II GAGCTC 2 cut(s) 34, 354
Eco147I AGGCCT 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 36, 356
Eco47I GGWCC 1 cut(s) 23
Eco53kI GAGCTC 2 cut(s) 34, 354
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 2 cut(s) 34, 354
EcoRII CCWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 36, 356
FaiI YATR 2 cut(s) 134, 136
FalI AAGNNNNNCTT 2 cut(s) 112, 144
FauI CCCGC 1 cut(s) 279
FauNDI CATATG 1 cut(s) 134
Fnu4HI GCNGC 2 cut(s) 68, 140
FriOI GRGCYC 2 cut(s) 36, 356
Fsp4HI GCNGC 2 cut(s) 68, 140
FspBI CTAG 1 cut(s) 245
GluI GCNGC 2 cut(s) 68, 140
HaeIII GGCC 2 cut(s) 99, 339
HapII CCGG 5 cut(s) 224, 238, 303, 327, 345
HgaI GACGC 1 cut(s) 177
HinfI GANTC 4 cut(s) 83, 323, 369, 409
HpaII CCGG 5 cut(s) 224, 238, 303, 327, 345
HphI GGTGA 1 cut(s) 39
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 4 cut(s) 117, 334, 358, 374
Hpy188III TCNNGA 4 cut(s) 29, 214, 245, 327
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 2 cut(s) 321, 338
HpyAV CCTTC 3 cut(s) 118, 202, 268
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 180, 300
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 306
HpySE526I ACGT 1 cut(s) 258
Kpn2I TCCGGA 1 cut(s) 326
Kzo9I GATC 1 cut(s) 329
LmnI GCTCC 4 cut(s) 10, 39, 155, 359
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 206
MalI GATC 1 cut(s) 331
MboI GATC 1 cut(s) 329
MboII GAAGA 7 cut(s) 187, 277, 373, 376, 387, 412, 415
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 2 cut(s) 36, 356
MmeI TCCRAC 1 cut(s) 357
MroI TCCGGA 1 cut(s) 326
MspI CCGG 5 cut(s) 224, 238, 303, 327, 345
MspR9I CCNGG 2 cut(s) 101, 346
MvaI CCWGG 1 cut(s) 101
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 156, 306
NciI CCSGG 1 cut(s) 346
NdeI CATATG 1 cut(s) 134
NdeII GATC 1 cut(s) 329
NlaIV GGNNCC 3 cut(s) 6, 151, 331
PceI AGGCCT 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PfeI GAWTC 4 cut(s) 83, 323, 369, 409
PkrI GCNGC 2 cut(s) 69, 141
Psp124BI GAGCTC 2 cut(s) 36, 356
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 3 cut(s) 6, 151, 331
PspPI GGNCC 1 cut(s) 23
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SacI GAGCTC 2 cut(s) 36, 356
SatI GCNGC 2 cut(s) 68, 140
Sau3AI GATC 1 cut(s) 329
Sau96I GGNCC 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 101, 346
SduI GDGCHC 2 cut(s) 36, 356
SetI ASST 7 cut(s) 36, 62, 94, 186, 222, 261, 356
SinI GGWCC 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
SseBI AGGCCT 1 cut(s) 99
SsiI CCGC 5 cut(s) 65, 68, 139, 278, 286
SspMI CTAG 1 cut(s) 245
SstI GAGCTC 2 cut(s) 36, 356
StuI AGGCCT 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 99, 344
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 198, 350
TaqII GACCGA 1 cut(s) 11
TauI GCSGC 2 cut(s) 70, 142
TfiI GAWTC 4 cut(s) 83, 323, 369, 409
VpaK11BI GGWCC 1 cut(s) 23
XbaI TCTAGA 1 cut(s) 244
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.