Rh2BG235800

Protein SGT1 homolog At5g65490-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011479
Physical Location & Seq
Forward (+)
23483804 .. 23488933
5130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG235800.1

Sequence Viewer

Length: 1533 bp
ATGGCGGCACCTCCGGACCTGGACCCTTCCTCCTCCTCCATATTCTCCCACAAAAACTCCCGCCTCCCAGACGACACCGTTTTCTACGCCATCTTCCCGGACTCCTCCCTCACCTCCACCTCCGCCTCCGCCGCCTCGCTCCACTCCCTCCACCTCCAAATCCTCCAAACCCTAACCCCCTTCACCTCCGACTACATTTGGCAGCACGAGCCCTTCACTCTTTCCCCCTCCGCCTCCCCCAAGCCCTCCTGCCTCTGCTCCCCCCACCTCCCTCACCTCCACGGCAAGCTCCGCGTCGGCGACAACCTCGACGACGAGTGGTTCACCGTCTTCCTCCTCTTCCACATCTCCTCCACCTTCCCGGACCTCTCCATCCGAGTCTGGGACTCCGACGGCGAGTTTCTCCTAATTGAAGCCGCCTTTCACCTCCCTCGCTGGCTCAACCCTCAGACCAGCTCCAACCGCGTCTTCATTCGCCGCGGTCAGGTTCATATCGTCCCCCGGCACCGCCTCCGGAATCCTAACCTGACAGAGGCGTTGAATTTCATTGCGGCGTTTGGTGAGGAGTCGGTTGCGGCTGAGCCGGTGCAGGCTGCGGTGAAGAACCGGATTTCGGAGTATCCGGAGAAGGCGAGGAGGAATATGCATGTGGTTAGGGTTAGGGTTCCGCCGGCGGTGGCTCAGGTGTTGAGTCAGGAGCCGTGTCTGGTTGCATTGGCGGTTGAGGGCTTCTATGACCGTGACATTGATACAATGAAGTATGCGGCGAAGATGGAGAGGTTTCTGAGCAGAGGGAGGGAGGAGGAATTGGTGTGCATATCGGTGAAAATGTCGAGGGCAATGTATGCTCAGCTGGTGCAGCAGACGTTTCAGGCGCCGAAATGTTATCCGATGATGCCGAATAGGAGCGATTCGGCATATGTGGAGGCTGAATTGGGAATGAAGATTGCGTGTGGGTTGGAGATGATGTATCAGCATAGGAGAAGGGAGGGGTTGGAAGGGAAAGGGAGTACGTGGGAGGCCTTTAGGGAGAGTTTGGAGAGTAGCGGTTACTTTGAAGGGTTGCTTCCGGGTTCAAAAGAGTACCAGAGGTTGATGCAGAATGCGGAGGAGTATTACCGGAGTAGTGCTTCGTTTTCAAGGGCTAGTGAGATGATGAGTGCTCCAGTGAGGCGCATAGATGAGATTCTTGCTTTACCTTATTCGGTGGATGATTTTAAGCGTGTAGATGTTCCACCTTCTGATGATGATTCTTGGCTTTACAATGGAGAGGATGAATTGAACTCTGAACTTCTAGAGAGACAAAAGGAGATGGAACTTTATGATTTAAAACATAAGAAGAAGCAGAAGTCAAAAGAGCAGGACAATGTTGGTCCATCCTCCAGCTCTAACACTGATGGGTTCAATCCTGGTGATATAGCAAAAACCATGCAGGCCTTTGTCGAAAAGGTGTCAAGCTACAAGGGAGCTGAGGTTCCTGAGAACAGGTTAGTGCCTATCTGTTACTTATGTTTGTGTATCAGTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SGT1

Protein Analysis

510

Amino Acids

57.73

Weight (kDa)

5.69

Isoelectric Point (pI)

63.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SGT1 PF07093 27 - 496 1e-170 SGT1 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 7, 504, 874
AccII CGCG 3 cut(s) 294, 465, 480
AccIII TCCGGA 3 cut(s) 13, 513, 622
AcsI RAATTY 1 cut(s) 541
AcyI GRCGYC 1 cut(s) 875
AfaI GTAC 2 cut(s) 1012, 1085
AfiI CCNNNNNNNGG 4 cut(s) 382, 484, 532, 613
AgsI TTSAA 7 cut(s) 413, 541, 1058, 1077, 1140, 1282, 1405
AjnI CCWGG 2 cut(s) 18, 1408
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AloI GAACNNNNNNTCC 2 cut(s) 1458, 1490
AluBI AGCT 6 cut(s) 289, 456, 853, 1386, 1458, 1469
AluI AGCT 6 cut(s) 289, 456, 853, 1386, 1458, 1469
Alw21I GWGCWC 1 cut(s) 1165
Alw26I GTCTC 1 cut(s) 1294
Aor13HI TCCGGA 3 cut(s) 13, 513, 622
AoxI GGCC 2 cut(s) 1020, 1434
ApeKI GCWGC 3 cut(s) 202, 593, 859
ApoI RAATTY 1 cut(s) 541
AspLEI GCGC 2 cut(s) 877, 1176
AspS9I GGNCC 4 cut(s) 16, 22, 364, 1373
AsuC2I CCSGG 4 cut(s) 98, 362, 502, 1071
AsuHPI GGTGA 9 cut(s) 103, 175, 266, 316, 416, 572, 610, 835, 1424
AvaII GGWCC 4 cut(s) 16, 22, 364, 1373
BanI GGYRCC 3 cut(s) 7, 504, 874
BanII GRGCYC 1 cut(s) 213
BarI GAAGNNNNNNTAC 1 cut(s) 1510
BauI CACGAG 1 cut(s) 206
BbsI GAAGAC 2 cut(s) 322, 460
Bbv12I GWGCWC 1 cut(s) 1165
BbvCI CCTCAGC 1 cut(s) 1470
BbvI GCAGC 3 cut(s) 214, 580, 871
BccI CCATC 6 cut(s) 98, 380, 766, 1306, 1384, 1391
BceAI ACGGC 3 cut(s) 298, 409, 685
BciT130I CCWGG 2 cut(s) 20, 1410
BciVI GTATCC 1 cut(s) 630
BcnI CCSGG 4 cut(s) 98, 362, 502, 1071
BcoDI GTCTC 1 cut(s) 1294
BfaI CTAG 2 cut(s) 1146, 1295
BfoI RGCGCY 1 cut(s) 878
BfuI GTATCC 1 cut(s) 630
BlpI GCTNAGC 2 cut(s) 579, 849
Bme1390I CCNGG 6 cut(s) 20, 98, 362, 502, 1071, 1410
Bme18I GGWCC 4 cut(s) 16, 22, 364, 1373
BmgT120I GGNCC 4 cut(s) 16, 22, 364, 1373
BmiI GGNNCC 7 cut(s) 9, 24, 506, 666, 699, 876, 1476
BmrFI CCNGG 6 cut(s) 20, 98, 362, 502, 1071, 1410
BmsI GCATC 2 cut(s) 885, 1086
BpiI GAAGAC 2 cut(s) 322, 460
BpmI CTGGAG 2 cut(s) 1149, 1366
Bpu10I CCTNAGC 2 cut(s) 681, 1470
Bpu1102I GCTNAGC 2 cut(s) 579, 849
BpuMI CCSGG 4 cut(s) 98, 362, 502, 1071
BsaAI YACGTR 1 cut(s) 1014
BsaHI GRCGYC 1 cut(s) 875
BsaJI CCNNGG 3 cut(s) 280, 478, 500
BsaWI WCCGGW 5 cut(s) 13, 513, 606, 622, 1119
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 484, 532, 613
Bse118I RCCGGY 2 cut(s) 583, 670
Bse1I ACTGG 1 cut(s) 1166
Bse3DI GCAATG 2 cut(s) 546, 846
BseAI TCCGGA 3 cut(s) 13, 513, 622
BseBI CCWGG 2 cut(s) 20, 1410
BseDI CCNNGG 3 cut(s) 280, 478, 500
BseGI GGATG 4 cut(s) 372, 1216, 1279, 1376
BseLI CCNNNNNNNGG 4 cut(s) 382, 484, 532, 613
BseMI GCAATG 2 cut(s) 546, 846
BseMII CTCAG 7 cut(s) 461, 570, 695, 776, 863, 1461, 1470
BseNI ACTGG 1 cut(s) 1166
BseRI GAGGAG 9 cut(s) 22, 25, 94, 326, 340, 578, 649, 815, 1124
BseXI GCAGC 3 cut(s) 214, 580, 871
BsgI GTGCAG 2 cut(s) 608, 878
Bsh1236I CGCG 3 cut(s) 294, 465, 480
BshFI GGCC 2 cut(s) 1022, 1436
BshNI GGYRCC 3 cut(s) 7, 504, 874
BsiHKAI GWGCWC 1 cut(s) 1165
BslFI GGGAC 2 cut(s) 398, 482
BslI CCNNNNNNNGG 4 cut(s) 382, 484, 532, 613
BsmAI GTCTC 1 cut(s) 1294
BsmFI GGGAC 2 cut(s) 398, 482
BsmI GAATGC 1 cut(s) 1108
BsnI GGCC 2 cut(s) 1022, 1436
Bsp1286I GDGCHC 2 cut(s) 213, 1165
Bsp13I TCCGGA 3 cut(s) 13, 513, 622
Bsp1720I GCTNAGC 2 cut(s) 579, 849
BspANI GGCC 2 cut(s) 1022, 1436
BspCNI CTCAG 7 cut(s) 460, 571, 694, 777, 862, 1462, 1471
BspEI TCCGGA 3 cut(s) 13, 513, 622
BspFNI CGCG 3 cut(s) 294, 465, 480
BspHI TCATGA 1 cut(s) 1529
BspLI GGNNCC 7 cut(s) 9, 24, 506, 666, 699, 876, 1476
BspT107I GGYRCC 3 cut(s) 7, 504, 874
BsrDI GCAATG 2 cut(s) 546, 846
BsrFI RCCGGY 2 cut(s) 583, 670
BsrI ACTGG 1 cut(s) 1166
BssAI RCCGGY 2 cut(s) 583, 670
BssECI CCNNGG 3 cut(s) 280, 478, 500
BssNI GRCGYC 1 cut(s) 875
BssSI CACGAG 1 cut(s) 206
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 20, 1410
Bst4CI ACNGT 3 cut(s) 79, 328, 740
Bst6I CTCTTC 1 cut(s) 344
BstACI GRCGYC 1 cut(s) 875
BstAPI GCANNNNNTGC 1 cut(s) 845
BstBAI YACGTR 1 cut(s) 1014
BstC8I GCNNGC 5 cut(s) 287, 437, 591, 672, 1434
BstDEI CTNAG 7 cut(s) 447, 579, 681, 785, 849, 1470, 1479
BstDSI CCRYGG 2 cut(s) 280, 478
BstENI CCTNNNNNAGG 1 cut(s) 530
BstF5I GGATG 4 cut(s) 372, 1216, 1279, 1376
BstFNI CGCG 3 cut(s) 294, 465, 480
BstH2I RGCGCY 1 cut(s) 878
BstHHI GCGC 2 cut(s) 877, 1176
BstMAI GTCTC 1 cut(s) 1294
BstMWI GCNNNNNNNGC 6 cut(s) 131, 208, 291, 462, 845, 859
BstNI CCWGG 2 cut(s) 20, 1410
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 6 cut(s) 18, 96, 360, 500, 1069, 1408
BstUI CGCG 3 cut(s) 294, 465, 480
BstV1I GCAGC 3 cut(s) 214, 580, 871
BstV2I GAAGAC 2 cut(s) 322, 460
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 2 cut(s) 1022, 1436
BtgI CCRYGG 2 cut(s) 280, 478
BtsCI GGATG 4 cut(s) 372, 1216, 1279, 1376
BtsIMutI CAGTG 2 cut(s) 1173, 1392
Cac8I GCNNGC 5 cut(s) 287, 437, 591, 672, 1434
CciI TCATGA 1 cut(s) 1529
CfoI GCGC 2 cut(s) 877, 1176
Cfr10I RCCGGY 2 cut(s) 583, 670
Cfr13I GGNCC 4 cut(s) 16, 22, 364, 1373
Cfr42I CCGCGG 1 cut(s) 481
CseI GACGC 2 cut(s) 283, 454
Csp6I GTAC 2 cut(s) 1011, 1084
CviAII CATG 3 cut(s) 647, 1429, 1530
CviQI GTAC 2 cut(s) 1011, 1084
DdeI CTNAG 7 cut(s) 447, 579, 681, 785, 849, 1470, 1479
DinI GGCGCC 1 cut(s) 876
DraI TTTAAA 1 cut(s) 1329
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
EciI GGCGGA 4 cut(s) 112, 118, 220, 657
Eco147I AGGCCT 2 cut(s) 1022, 1436
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 4 cut(s) 16, 22, 364, 1373
EcoNI CCTNNNNNAGG 1 cut(s) 530
EcoRII CCWGG 2 cut(s) 18, 1408
EcoT22I ATGCAT 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 213
EgeI GGCGCC 1 cut(s) 876
EheI GGCGCC 1 cut(s) 876
FaeI CATG 3 cut(s) 650, 1432, 1533
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 2 cut(s) 398, 482
FatI CATG 3 cut(s) 646, 1428, 1529
FauI CCCGC 1 cut(s) 68
FauNDI CATATG 1 cut(s) 919
FokI GGATG 4 cut(s) 359, 1223, 1286, 1363
FriOI GRGCYC 1 cut(s) 213
FspBI CTAG 2 cut(s) 1146, 1295
GlaI GCGC 2 cut(s) 876, 1175
GsuI CTGGAG 2 cut(s) 1149, 1366
HaeII RGCGCY 1 cut(s) 878
HaeIII GGCC 2 cut(s) 1022, 1436
HgaI GACGC 2 cut(s) 283, 454
HhaI GCGC 2 cut(s) 877, 1176
Hin1I GRCGYC 1 cut(s) 875
Hin1II CATG 3 cut(s) 650, 1432, 1533
Hin6I GCGC 2 cut(s) 875, 1174
HinP1I GCGC 2 cut(s) 875, 1174
HinfI GANTC 9 cut(s) 101, 378, 386, 517, 566, 691, 911, 1186, 1250
HphI GGTGA 9 cut(s) 103, 175, 266, 316, 416, 572, 610, 835, 1424
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 9 cut(s) 190, 377, 391, 450, 616, 786, 891, 1243, 1288
Hpy188III TCNNGA 7 cut(s) 14, 514, 623, 695, 1295, 1478, 1530
Hpy8I GTNNAC 1 cut(s) 324
Hpy99I CGWCG 4 cut(s) 299, 314, 317, 395
HpyAV CCTTC 9 cut(s) 36, 190, 223, 367, 622, 978, 992, 1052, 1248
HpyCH4III ACNGT 3 cut(s) 79, 328, 740
HpyCH4IV ACGT 2 cut(s) 866, 1013
HpyCH4V TGCA 7 cut(s) 589, 646, 713, 816, 859, 1099, 1432
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 208, 291, 462, 845, 859
HpyF3I CTNAG 7 cut(s) 447, 579, 681, 785, 849, 1470, 1479
HpySE526I ACGT 2 cut(s) 866, 1013
Hsp92I GRCGYC 1 cut(s) 875
Hsp92II CATG 3 cut(s) 650, 1432, 1533
HspAI GCGC 2 cut(s) 875, 1174
KasI GGCGCC 1 cut(s) 874
Kpn2I TCCGGA 3 cut(s) 13, 513, 622
KroI GCCGGC 1 cut(s) 670
KroNI GCCGGC 1 cut(s) 672
KspI CCGCGG 1 cut(s) 481
LmnI GCTCC 8 cut(s) 144, 263, 294, 461, 697, 906, 1168, 1466
Lsp1109I GCAGC 3 cut(s) 214, 580, 871
LweI GCATC 2 cut(s) 885, 1086
MaeI CTAG 2 cut(s) 1146, 1295
MaeII ACGT 2 cut(s) 866, 1013
MaeIII GTNAC 3 cut(s) 740, 1049, 1502
MboII GAAGA 9 cut(s) 85, 322, 331, 460, 613, 781, 955, 1351, 1518
MhlI GDGCHC 2 cut(s) 213, 1165
MluCI AATT 5 cut(s) 408, 541, 806, 932, 1277
Mly113I GGCGCC 1 cut(s) 875
MlyI GAGTC 5 cut(s) 95, 380, 387, 575, 700
MmeI TCCRAC 5 cut(s) 213, 414, 483, 939, 975
Mph1103I ATGCAT 1 cut(s) 648
MreI CGCCGGCG 1 cut(s) 670
MroI TCCGGA 3 cut(s) 13, 513, 622
MroNI GCCGGC 1 cut(s) 670
MseI TTAA 2 cut(s) 1218, 1328
MslI CAYNNNNRTG 1 cut(s) 821
MspA1I CMGCKG 2 cut(s) 480, 853
MspR9I CCNGG 6 cut(s) 20, 98, 362, 502, 1071, 1410
Mva1269I GAATGC 1 cut(s) 1108
MvaI CCWGG 2 cut(s) 20, 1410
MvnI CGCG 3 cut(s) 294, 465, 480
MwoI GCNNNNNNNGC 6 cut(s) 131, 208, 291, 462, 845, 859
NaeI GCCGGC 1 cut(s) 672
NarI GGCGCC 1 cut(s) 875
NciI CCSGG 4 cut(s) 98, 362, 502, 1071
NdeI CATATG 1 cut(s) 919
NgoMIV GCCGGC 1 cut(s) 670
NlaIII CATG 3 cut(s) 650, 1432, 1533
NlaIV GGNNCC 7 cut(s) 9, 24, 506, 666, 699, 876, 1476
NmuCI GTSAC 1 cut(s) 740
NsiI ATGCAT 1 cut(s) 648
NspI RCATGY 1 cut(s) 650
PagI TCATGA 1 cut(s) 1529
PceI AGGCCT 2 cut(s) 1022, 1436
PctI GAATGC 1 cut(s) 1108
PdiI GCCGGC 1 cut(s) 672
PfeI GAWTC 4 cut(s) 517, 911, 1186, 1250
PfoI TCCNGGA 2 cut(s) 96, 360
PleI GAGTC 5 cut(s) 95, 380, 386, 574, 699
PluTI GGCGCC 1 cut(s) 878
PpsI GAGTC 5 cut(s) 95, 380, 386, 574, 699
Ppu21I YACGTR 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 18, 1408
PspGI CCWGG 2 cut(s) 18, 1408
PspN4I GGNNCC 7 cut(s) 9, 24, 506, 666, 699, 876, 1476
PspPI GGNCC 4 cut(s) 16, 22, 364, 1373
PvuII CAGCTG 1 cut(s) 853
RsaI GTAC 2 cut(s) 1012, 1085
RsaNI GTAC 2 cut(s) 1011, 1084
RseI CAYNNNNRTG 1 cut(s) 821
SacII CCGCGG 1 cut(s) 481
SaqAI TTAA 2 cut(s) 1218, 1328
Sau96I GGNCC 4 cut(s) 16, 22, 364, 1373
SchI GAGTC 5 cut(s) 95, 380, 387, 575, 700
ScrFI CCNGG 6 cut(s) 20, 98, 362, 502, 1071, 1410
SduI GDGCHC 2 cut(s) 213, 1165
SfaNI GCATC 2 cut(s) 885, 1086
SfoI GGCGCC 1 cut(s) 876
Sfr303I CCGCGG 1 cut(s) 481
SgrAI CRCCGGYG 1 cut(s) 670
SgrBI CCGCGG 1 cut(s) 481
SinI GGWCC 4 cut(s) 16, 22, 364, 1373
SmiMI CAYNNNNRTG 1 cut(s) 821
Sse9I AATT 5 cut(s) 408, 541, 806, 932, 1277
SseBI AGGCCT 2 cut(s) 1022, 1436
SspDI GGCGCC 1 cut(s) 874
SspMI CTAG 2 cut(s) 1146, 1295
StuI AGGCCT 2 cut(s) 1022, 1436
StyD4I CCNGG 6 cut(s) 18, 96, 360, 500, 1069, 1408
TaaI ACNGT 3 cut(s) 79, 328, 740
TaiI ACGT 2 cut(s) 869, 1016
TaqI TCGA 3 cut(s) 309, 833, 1443
TasI AATT 5 cut(s) 408, 541, 806, 932, 1277
TauI GCSGC 7 cut(s) 8, 134, 419, 480, 554, 578, 767
TfiI GAWTC 4 cut(s) 517, 911, 1186, 1250
Tru1I TTAA 2 cut(s) 1218, 1328
Tru9I TTAA 2 cut(s) 1218, 1328
TscAI CASTG 2 cut(s) 1173, 1399
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 3 cut(s) 202, 593, 859
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 7 cut(s) 460, 479, 535, 770, 956, 1290, 1518
TspRI CASTG 2 cut(s) 1173, 1399
VpaK11BI GGWCC 4 cut(s) 16, 22, 364, 1373
XagI CCTNNNNNAGG 1 cut(s) 530
XapI RAATTY 1 cut(s) 541
XbaI TCTAGA 1 cut(s) 1294
XceI RCATGY 1 cut(s) 650
XspI CTAG 2 cut(s) 1146, 1295
Zsp2I ATGCAT 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.