Rh2DG231800

Protein SGT1 homolog At5g65490-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011479
Physical Location & Seq
Forward (+)
23009928 .. 23015886
5959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG231800.1

Sequence Viewer

Length: 1539 bp
ATGGCGGCACCTCCAGACCTGGACCCTTCCTCCTCCTCCTCCTCCATATTCTCCCACAAAAACTCCCGCCTCCCAGACGACACCGTTTTCTACGCCATCTTCCCGGACTCCTCCCTCACCCCCACCTCCACCACCGCCGCCTCGCTCCACTCCCTCCACCTCCAAATCCTCCAAACCCTAACCCCCTTCACCTCCGACTACATTTGGCAGCACGAGCCCTTCACTCTCTCCCCCTCCGCCTCCCCCAAGCCCTCCTGCCTCTGCTCCCCCCACCTCCCTCACCTCCACGGCAAGCTCCGCGTCGGCGACAACCTCGACGACGAGTGGTTCACCGTCTTCCTCCTCTTCCACATCTCCTCCACCTTCCCGGACCTCTCCATCCGAGTCTGGGACTCCGACGGCGAGTTTCTCCTAATTGAAGCCGCCTTTCACCTCCCTCGCTGGCTCAACCCTCAGACCAGCTCCAACCGCGTCTTCATTCGCCGCGGTCAGGTTCATATCGTCCCCCGGCACCGCCTCCGGAACCCTAACCTGACAGAGGCGTTGAATTTCATTGCGGCGTTTGGTGAGGAGTCGGTTGCGGCTCAGCCGGTGCAGGCTGCGGTGAAGAACCGGATTTCGGAGTATCCCGAGAAGGCGAGGAGGAATATGCATGTGGTTAGGGTTAGGGTTCCGCCGGCGGTGGCTCAGGTGTTGAGTCAGGAGCCGTGTCTGGTTGCATTGGCGGTTGAGGGCTTCTATGACCGTGACATTGATACAATGAAGTATGCGGCGCAGATGGAGAGGTTTCTGAGCAGAGGGAGGGAGGAGGAATTGGTGTGCATATCGGTGAAAATGTCGAGGGCAATGTATGCTCAGCTGGTGCAGCAGACGTTTCAGGCACCGAAATGTTATCCGATGATGCCGAATAGGAGCGATTCGGCATATGTGGAGGCTGAATTGGGAATGAAGATTGCGTGTGGGTTGGAGATGATGTATCAGCACAGGAGAAAGGAGGGGTTGGAAGGGAAAGGGAGTACGTGGGAGGCCTTTAGGGAGAGTTTGGAGAGGAGCGGTTACTTTGAAGGGTTGCTTCCGGGTTCAAAAGAGTACCAGAGGTTGATGCAGAATGCGGAGGAGTATTACCGGAGTAGTGCTTCGTTTTCAAGGGCTAGTGAGATGATGAGTGCTCCAGTGAGGCGCATAGATGAGATTCTTGCTTTACCTTATTCGGTGGATGATTTTAAGCGTGTAGATGTTCCACCTTCTGATGATGATTCTTGGCTTTACAATGGAGAGGATGAATTGAACTCTGAACTTCTAGAGAGACAAAAGGAGATGGAACTTTATGATTTAAAACATAAGAAGAAGCAGAAGTCAAAAGAGCAGGACAATGTTGGTCCATCCTCCAGCTCTAACACTGATGGGTTCAATCCTGGTGATATAGCAAAAACCATGCAGGCCTTTGTCGAAAAGGTGTCAAGCTACAAGGGAGCTGAGGTTCCTGAGAACAGGTTAGTGCCTATCTGTTACTTATGTTTGTGTATCAGTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SGT1

Protein Analysis

512

Amino Acids

58.0

Weight (kDa)

5.76

Isoelectric Point (pI)

62.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SGT1 PF07093 29 - 498 1.3e-170 SGT1 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 7, 510, 880
AccBSI CCGCTC 1 cut(s) 1053
AccII CGCG 3 cut(s) 300, 471, 486
AccIII TCCGGA 1 cut(s) 519
AcsI RAATTY 1 cut(s) 547
AfaI GTAC 2 cut(s) 1018, 1091
AfiI CCNNNNNNNGG 4 cut(s) 388, 490, 538, 619
AgsI TTSAA 7 cut(s) 419, 547, 1064, 1083, 1146, 1288, 1411
AjnI CCWGG 2 cut(s) 18, 1414
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AloI GAACNNNNNNTCC 2 cut(s) 1464, 1496
AluBI AGCT 6 cut(s) 295, 462, 859, 1392, 1464, 1475
AluI AGCT 6 cut(s) 295, 462, 859, 1392, 1464, 1475
Alw21I GWGCWC 1 cut(s) 1171
Alw26I GTCTC 1 cut(s) 1300
Ama87I CYCGRG 1 cut(s) 629
Aor13HI TCCGGA 1 cut(s) 519
AoxI GGCC 2 cut(s) 1026, 1440
ApeKI GCWGC 3 cut(s) 208, 599, 865
ApoI RAATTY 1 cut(s) 547
AspLEI GCGC 2 cut(s) 775, 1182
AspS9I GGNCC 3 cut(s) 22, 370, 1379
AsuC2I CCSGG 4 cut(s) 104, 368, 508, 1077
AsuHPI GGTGA 9 cut(s) 109, 181, 272, 322, 422, 578, 616, 841, 1430
AvaI CYCGRG 1 cut(s) 629
AvaII GGWCC 3 cut(s) 22, 370, 1379
BanI GGYRCC 3 cut(s) 7, 510, 880
BanII GRGCYC 1 cut(s) 219
BarI GAAGNNNNNNTAC 1 cut(s) 1516
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 2 cut(s) 328, 466
Bbv12I GWGCWC 1 cut(s) 1171
BbvCI CCTCAGC 1 cut(s) 1476
BbvI GCAGC 3 cut(s) 220, 586, 877
BccI CCATC 6 cut(s) 104, 386, 772, 1312, 1390, 1397
BceAI ACGGC 3 cut(s) 304, 415, 691
BciT130I CCWGG 2 cut(s) 20, 1416
BciVI GTATCC 1 cut(s) 636
BcnI CCSGG 4 cut(s) 104, 368, 508, 1077
BcoDI GTCTC 1 cut(s) 1300
BfaI CTAG 2 cut(s) 1152, 1301
BfuI GTATCC 1 cut(s) 636
BlpI GCTNAGC 2 cut(s) 585, 855
Bme1390I CCNGG 6 cut(s) 20, 104, 368, 508, 1077, 1416
Bme18I GGWCC 3 cut(s) 22, 370, 1379
BmeT110I CYCGRG 1 cut(s) 629
BmgT120I GGNCC 3 cut(s) 22, 370, 1379
BmiI GGNNCC 8 cut(s) 9, 24, 512, 524, 672, 705, 882, 1482
BmrFI CCNGG 6 cut(s) 20, 104, 368, 508, 1077, 1416
BmsI GCATC 2 cut(s) 891, 1092
BpiI GAAGAC 2 cut(s) 328, 466
BpmI CTGGAG 2 cut(s) 1155, 1372
Bpu10I CCTNAGC 2 cut(s) 687, 1476
Bpu1102I GCTNAGC 2 cut(s) 585, 855
BpuMI CCSGG 4 cut(s) 104, 368, 508, 1077
BsaAI YACGTR 1 cut(s) 1020
BsaJI CCNNGG 3 cut(s) 286, 484, 506
BsaWI WCCGGW 3 cut(s) 519, 612, 1125
Bsc4I CCNNNNNNNGG 4 cut(s) 388, 490, 538, 619
Bse118I RCCGGY 2 cut(s) 589, 676
Bse1I ACTGG 1 cut(s) 1172
Bse3DI GCAATG 2 cut(s) 552, 852
BseAI TCCGGA 1 cut(s) 519
BseBI CCWGG 2 cut(s) 20, 1416
BseDI CCNNGG 3 cut(s) 286, 484, 506
BseGI GGATG 4 cut(s) 378, 1222, 1285, 1382
BseLI CCNNNNNNNGG 4 cut(s) 388, 490, 538, 619
BseMI GCAATG 2 cut(s) 552, 852
BseMII CTCAG 7 cut(s) 467, 599, 701, 782, 869, 1467, 1476
BseNI ACTGG 1 cut(s) 1172
BseXI GCAGC 3 cut(s) 220, 586, 877
BsgI GTGCAG 2 cut(s) 614, 884
Bsh1236I CGCG 3 cut(s) 300, 471, 486
BshFI GGCC 2 cut(s) 1028, 1442
BshNI GGYRCC 3 cut(s) 7, 510, 880
BsiHKAI GWGCWC 1 cut(s) 1171
BsiHKCI CYCGRG 1 cut(s) 629
BsiSI CCGG 9 cut(s) 104, 368, 508, 520, 590, 613, 677, 1076, 1126
BslFI GGGAC 2 cut(s) 404, 488
BslI CCNNNNNNNGG 4 cut(s) 388, 490, 538, 619
BsmAI GTCTC 1 cut(s) 1300
BsmFI GGGAC 2 cut(s) 404, 488
BsmI GAATGC 1 cut(s) 1114
BsnI GGCC 2 cut(s) 1028, 1442
BsoBI CYCGRG 1 cut(s) 629
Bsp1286I GDGCHC 2 cut(s) 219, 1171
Bsp13I TCCGGA 1 cut(s) 519
Bsp1720I GCTNAGC 2 cut(s) 585, 855
BspANI GGCC 2 cut(s) 1028, 1442
BspCNI CTCAG 7 cut(s) 466, 598, 700, 783, 868, 1468, 1477
BspEI TCCGGA 1 cut(s) 519
BspFNI CGCG 3 cut(s) 300, 471, 486
BspHI TCATGA 1 cut(s) 1535
BspLI GGNNCC 8 cut(s) 9, 24, 512, 524, 672, 705, 882, 1482
BspT107I GGYRCC 3 cut(s) 7, 510, 880
BsrBI CCGCTC 1 cut(s) 1053
BsrDI GCAATG 2 cut(s) 552, 852
BsrFI RCCGGY 2 cut(s) 589, 676
BsrI ACTGG 1 cut(s) 1172
BssAI RCCGGY 2 cut(s) 589, 676
BssECI CCNNGG 3 cut(s) 286, 484, 506
BssSI CACGAG 1 cut(s) 212
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 2 cut(s) 20, 1416
Bst4CI ACNGT 3 cut(s) 85, 334, 746
Bst6I CTCTTC 1 cut(s) 350
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 1 cut(s) 1020
BstC8I GCNNGC 5 cut(s) 293, 443, 597, 678, 1440
BstDEI CTNAG 7 cut(s) 453, 585, 687, 791, 855, 1476, 1485
BstDSI CCRYGG 2 cut(s) 286, 484
BstENI CCTNNNNNAGG 1 cut(s) 536
BstF5I GGATG 4 cut(s) 378, 1222, 1285, 1382
BstFNI CGCG 3 cut(s) 300, 471, 486
BstHHI GCGC 2 cut(s) 775, 1182
BstMAI GTCTC 1 cut(s) 1300
BstMWI GCNNNNNNNGC 5 cut(s) 214, 297, 468, 851, 865
BstNI CCWGG 2 cut(s) 20, 1416
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 18, 102, 366, 506, 1075, 1414
BstUI CGCG 3 cut(s) 300, 471, 486
BstV1I GCAGC 3 cut(s) 220, 586, 877
BstV2I GAAGAC 2 cut(s) 328, 466
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 2 cut(s) 1028, 1442
BtgI CCRYGG 2 cut(s) 286, 484
BtsCI GGATG 4 cut(s) 378, 1222, 1285, 1382
BtsIMutI CAGTG 2 cut(s) 1179, 1398
Cac8I GCNNGC 5 cut(s) 293, 443, 597, 678, 1440
CciI TCATGA 1 cut(s) 1535
CfoI GCGC 2 cut(s) 775, 1182
Cfr10I RCCGGY 2 cut(s) 589, 676
Cfr13I GGNCC 3 cut(s) 22, 370, 1379
Cfr42I CCGCGG 1 cut(s) 487
CseI GACGC 2 cut(s) 289, 460
Csp6I GTAC 2 cut(s) 1017, 1090
CviAII CATG 3 cut(s) 653, 1435, 1536
CviQI GTAC 2 cut(s) 1017, 1090
DdeI CTNAG 7 cut(s) 453, 585, 687, 791, 855, 1476, 1485
DraI TTTAAA 1 cut(s) 1335
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
EciI GGCGGA 2 cut(s) 226, 663
Eco147I AGGCCT 2 cut(s) 1028, 1442
Eco24I GRGCYC 1 cut(s) 219
Eco47I GGWCC 3 cut(s) 22, 370, 1379
Eco88I CYCGRG 1 cut(s) 629
EcoNI CCTNNNNNAGG 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 18, 1414
EcoT22I ATGCAT 1 cut(s) 654
EcoT38I GRGCYC 1 cut(s) 219
FaeI CATG 3 cut(s) 656, 1438, 1539
FalI AAGNNNNNCTT 2 cut(s) 1056, 1088
FaqI GGGAC 2 cut(s) 404, 488
FatI CATG 3 cut(s) 652, 1434, 1535
FauI CCCGC 1 cut(s) 74
FauNDI CATATG 1 cut(s) 925
FokI GGATG 4 cut(s) 365, 1229, 1292, 1369
FriOI GRGCYC 1 cut(s) 219
FspBI CTAG 2 cut(s) 1152, 1301
GlaI GCGC 2 cut(s) 774, 1181
GsuI CTGGAG 2 cut(s) 1155, 1372
HaeIII GGCC 2 cut(s) 1028, 1442
HapII CCGG 9 cut(s) 104, 368, 508, 520, 590, 613, 677, 1076, 1126
HgaI GACGC 2 cut(s) 289, 460
HhaI GCGC 2 cut(s) 775, 1182
Hin1II CATG 3 cut(s) 656, 1438, 1539
Hin6I GCGC 2 cut(s) 773, 1180
HinP1I GCGC 2 cut(s) 773, 1180
HinfI GANTC 8 cut(s) 107, 384, 392, 572, 697, 917, 1192, 1256
HpaII CCGG 9 cut(s) 104, 368, 508, 520, 590, 613, 677, 1076, 1126
HphI GGTGA 9 cut(s) 109, 181, 272, 322, 422, 578, 616, 841, 1430
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 9 cut(s) 196, 383, 397, 456, 622, 792, 897, 1249, 1294
Hpy188III TCNNGA 7 cut(s) 14, 520, 629, 701, 1301, 1484, 1536
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 4 cut(s) 305, 320, 323, 401
HpyAV CCTTC 8 cut(s) 36, 196, 229, 373, 628, 998, 1058, 1254
HpyCH4III ACNGT 3 cut(s) 85, 334, 746
HpyCH4IV ACGT 2 cut(s) 872, 1019
HpyCH4V TGCA 7 cut(s) 595, 652, 719, 822, 865, 1105, 1438
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 297, 468, 851, 865
HpyF3I CTNAG 7 cut(s) 453, 585, 687, 791, 855, 1476, 1485
HpySE526I ACGT 2 cut(s) 872, 1019
Hsp92II CATG 3 cut(s) 656, 1438, 1539
HspAI GCGC 2 cut(s) 773, 1180
Kpn2I TCCGGA 1 cut(s) 519
KroI GCCGGC 1 cut(s) 676
KroNI GCCGGC 1 cut(s) 678
KspI CCGCGG 1 cut(s) 487
LmnI GCTCC 9 cut(s) 150, 269, 300, 467, 703, 912, 1050, 1174, 1472
Lsp1109I GCAGC 3 cut(s) 220, 586, 877
LweI GCATC 2 cut(s) 891, 1092
MaeI CTAG 2 cut(s) 1152, 1301
MaeII ACGT 2 cut(s) 872, 1019
MaeIII GTNAC 3 cut(s) 746, 1055, 1508
MbiI CCGCTC 1 cut(s) 1053
MboII GAAGA 8 cut(s) 91, 328, 337, 466, 619, 961, 1357, 1524
MhlI GDGCHC 2 cut(s) 219, 1171
MluCI AATT 5 cut(s) 414, 547, 812, 938, 1283
MlyI GAGTC 5 cut(s) 101, 386, 393, 581, 706
MmeI TCCRAC 5 cut(s) 219, 420, 489, 945, 981
Mph1103I ATGCAT 1 cut(s) 654
MreI CGCCGGCG 1 cut(s) 676
MroI TCCGGA 1 cut(s) 519
MroNI GCCGGC 1 cut(s) 676
MseI TTAA 2 cut(s) 1224, 1334
MslI CAYNNNNRTG 2 cut(s) 827, 886
MspA1I CMGCKG 2 cut(s) 486, 859
MspI CCGG 9 cut(s) 104, 368, 508, 520, 590, 613, 677, 1076, 1126
MspR9I CCNGG 6 cut(s) 20, 104, 368, 508, 1077, 1416
Mva1269I GAATGC 1 cut(s) 1114
MvaI CCWGG 2 cut(s) 20, 1416
MvnI CGCG 3 cut(s) 300, 471, 486
MwoI GCNNNNNNNGC 5 cut(s) 214, 297, 468, 851, 865
NaeI GCCGGC 1 cut(s) 678
NciI CCSGG 4 cut(s) 104, 368, 508, 1077
NdeI CATATG 1 cut(s) 925
NgoMIV GCCGGC 1 cut(s) 676
NlaIII CATG 3 cut(s) 656, 1438, 1539
NlaIV GGNNCC 8 cut(s) 9, 24, 512, 524, 672, 705, 882, 1482
NmuCI GTSAC 1 cut(s) 746
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 1 cut(s) 656
PagI TCATGA 1 cut(s) 1535
PceI AGGCCT 2 cut(s) 1028, 1442
PctI GAATGC 1 cut(s) 1114
PdiI GCCGGC 1 cut(s) 678
PfeI GAWTC 3 cut(s) 917, 1192, 1256
PfoI TCCNGGA 2 cut(s) 102, 366
PleI GAGTC 5 cut(s) 101, 386, 392, 580, 705
PpsI GAGTC 5 cut(s) 101, 386, 392, 580, 705
Ppu21I YACGTR 1 cut(s) 1020
Psp6I CCWGG 2 cut(s) 18, 1414
PspGI CCWGG 2 cut(s) 18, 1414
PspN4I GGNNCC 8 cut(s) 9, 24, 512, 524, 672, 705, 882, 1482
PspPI GGNCC 3 cut(s) 22, 370, 1379
PvuII CAGCTG 1 cut(s) 859
RsaI GTAC 2 cut(s) 1018, 1091
RsaNI GTAC 2 cut(s) 1017, 1090
RseI CAYNNNNRTG 2 cut(s) 827, 886
SacII CCGCGG 1 cut(s) 487
SaqAI TTAA 2 cut(s) 1224, 1334
Sau96I GGNCC 3 cut(s) 22, 370, 1379
SchI GAGTC 5 cut(s) 101, 386, 393, 581, 706
ScrFI CCNGG 6 cut(s) 20, 104, 368, 508, 1077, 1416
SduI GDGCHC 2 cut(s) 219, 1171
SfaNI GCATC 2 cut(s) 891, 1092
Sfr303I CCGCGG 1 cut(s) 487
SgrAI CRCCGGYG 1 cut(s) 676
SgrBI CCGCGG 1 cut(s) 487
SinI GGWCC 3 cut(s) 22, 370, 1379
SmiMI CAYNNNNRTG 2 cut(s) 827, 886
Sse9I AATT 5 cut(s) 414, 547, 812, 938, 1283
SseBI AGGCCT 2 cut(s) 1028, 1442
SspMI CTAG 2 cut(s) 1152, 1301
StuI AGGCCT 2 cut(s) 1028, 1442
StyD4I CCNGG 6 cut(s) 18, 102, 366, 506, 1075, 1414
TaaI ACNGT 3 cut(s) 85, 334, 746
TaiI ACGT 2 cut(s) 875, 1022
TaqI TCGA 3 cut(s) 315, 839, 1449
TasI AATT 5 cut(s) 414, 547, 812, 938, 1283
TauI GCSGC 7 cut(s) 8, 140, 425, 486, 560, 584, 773
TfiI GAWTC 3 cut(s) 917, 1192, 1256
Tru1I TTAA 2 cut(s) 1224, 1334
Tru9I TTAA 2 cut(s) 1224, 1334
TscAI CASTG 2 cut(s) 1179, 1405
TseFI GTSAC 1 cut(s) 746
TseI GCWGC 3 cut(s) 208, 599, 865
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 7 cut(s) 466, 485, 541, 776, 962, 1296, 1524
TspRI CASTG 2 cut(s) 1179, 1405
VpaK11BI GGWCC 3 cut(s) 22, 370, 1379
XagI CCTNNNNNAGG 1 cut(s) 536
XapI RAATTY 1 cut(s) 547
XbaI TCTAGA 1 cut(s) 1300
XceI RCATGY 1 cut(s) 656
XspI CTAG 2 cut(s) 1152, 1301
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.