Rh2BG489400

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004676
Physical Location & Seq
Reverse (-)
68921231 .. 68927485
6255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG489400.1

Sequence Viewer

Length: 1539 bp
ATGTTGACGAGCATCGTCACATTACTTGCTCCTTCTTCACAACGAAGTGAGAGAGAGAGAGAGAGAGACGATACCACATTTCCGATGGATCAACTTTTCTCGGCGAAGCCTAAACCCTTCCGTATCCACAGAAAATCACCTATCGCTTCCAAGATCAAATCCTCCATCTCCGTCAAGCTCTCTGAATTTCTCGGTGGTTACACTGACGACGTCTTCCTGGAGTATATTACAGTGCTGATTTGTAATGGAAAGGATCAATATCAGGCAAGAGATGACTTAGAGGCATTTCTTGGTGAGAGGAGTGAAGAATTTGTATCATGGTTATGGGACCTTCTCTTCAAACTTGCTGGACAGTTTTGTAAGGATACTGACTATCTCGATGAAGTTCCTGGCATTAGTCCCCCAAACAGTGATAGCAGCATTGGTGCAAAAGTTGACAGGTGTACAGACTTCCGGAGCCATGTTAACGAACTTTTGCAGATGAGGGGTGATAAAATGCGACATTCTGCTTGTGATCCTGCTTCCTGTAACGATATGAAGCTTGCCAAAGGTTCTCAAGATTACCCCTCTGTCTCTGTCCCTTCAACTGAGGTGAATACAGCGTTGTCACATGGATGTAGAAATGAAATTCTGAGAAGATATATGGCAAGTGGAGATATTGATGGTGAGGGAGATCGAAGAACTCAGTCTAAAAATCAGATTACCCAGATTGTCAGTCATAAATGCAGTGGATTGCCTAGAGAGCGGTTACATTTACCAGAAAGAGAGGCCATGTCTAAGAATACAGAATTGTCCGTATCAGGACGCCAATTTTCTGCACAGATTTGTGCCAGTAACAAGATAGGAACTAATCTGTCACAAAGATCTATAGGTGCAGGATCCATACGTAGTAAAAAATCTCGGGGTAGTGTTTGGGATAGATTGGGCAAGCCATGTGAAGACATATCTGGGGGACTCAAGAGTGTTGATTTTTACGGTGCCGGGCATAACAAGCAATGTGACAAAGTTAATCAGCTTGCATTAGTACCTCAGAAGAGGAATTCTGAAGTTCCTGCTTTTGATAAGAAGAGGCTGTACAGTAATCCTGTTGAGATGGAGAAACTGGATCATGTTGATAATGCAGTGAGTGAACCTCATTTTGCAAATAATATTGCCCGGAAGAGGCTCTTTGGTCAAATCAGTCCTGAATGCGAAGATACCTCACAGCATTTTAAGAAATCAGATGTGGCCACAAATGATTTGAAGACCATACAGGAATTGCTTCATGTGAAAGAAAAATTGAATCAAGTTGAAATCGAAATGTCAAAGCTTCGGTCAAAGCAAGTGGAGTTTGAAAAAAATGATGGGAAAACAAATCACTTGCTGAATTCTGGGGCACCGAAAGATTCAGAGGAAACTTTTGAGTCAAGAACTGTGTTGGTGACAAATGTACATTTTGCAGCAACTAGAGAAGCTTTGTCCCTGTACTTTGCCAAGTGTGGAATGATTGTTAACGTAATGATCTTGACAGACACAGTGACTTCAAAGCCAAAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWI RRM_1

Protein Analysis

512

Amino Acids

57.34

Weight (kDa)

8.15

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWI PF01480 51 - 114 1.1e-07 PWI domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 213
AccB1I GGYRCC 2 cut(s) 977, 1375
AccBSI CCGCTC 1 cut(s) 745
AccIII TCCGGA 1 cut(s) 453
AciI CCGC 1 cut(s) 745
AclWI GGATC 6 cut(s) 96, 261, 509, 873, 886, 1113
AcoI YGGCCR 1 cut(s) 1227
AcsI RAATTY 5 cut(s) 185, 308, 627, 1039, 1366
AcuI CTGAAG 1 cut(s) 1065
AcyI GRCGYC 2 cut(s) 210, 805
AfaI GTAC 5 cut(s) 445, 1026, 1076, 1431, 1466
AfiI CCNNNNNNNGG 1 cut(s) 1161
AgsI TTSAA 7 cut(s) 340, 585, 1243, 1282, 1292, 1334, 1524
AjnI CCWGG 2 cut(s) 216, 388
AluBI AGCT 5 cut(s) 178, 541, 1015, 1309, 1454
AluI AGCT 5 cut(s) 178, 541, 1015, 1309, 1454
Alw26I GTCTC 2 cut(s) 60, 577
AlwI GGATC 6 cut(s) 96, 261, 509, 873, 886, 1113
Ama87I CYCGRG 1 cut(s) 900
Aor13HI TCCGGA 1 cut(s) 453
AoxI GGCC 2 cut(s) 768, 1227
ApeKI GCWGC 2 cut(s) 417, 1439
ApoI RAATTY 5 cut(s) 185, 308, 627, 1039, 1366
ArsI GACNNNNNNTTYG 2 cut(s) 1298, 1330
Asp700I GAANNNNTTC 1 cut(s) 1260
AspS9I GGNCC 1 cut(s) 328
AsuC2I CCSGG 2 cut(s) 982, 1156
AsuHPI GGTGA 6 cut(s) 129, 305, 500, 604, 677, 1432
AvaI CYCGRG 1 cut(s) 900
AvaII GGWCC 1 cut(s) 328
BaeGI GKGCMC 1 cut(s) 1378
BalI TGGCCA 1 cut(s) 1229
BamHI GGATCC 1 cut(s) 878
BanI GGYRCC 2 cut(s) 977, 1375
BarI GAAGNNNNNNTAC 2 cut(s) 1058, 1090
BbsI GAAGAC 3 cut(s) 205, 945, 1250
BbvI GCAGC 2 cut(s) 429, 1451
BccI CCATC 5 cut(s) 79, 173, 656, 1087, 1337
BciT130I CCWGG 2 cut(s) 218, 390
BciVI GTATCC 2 cut(s) 134, 358
BcnI CCSGG 2 cut(s) 982, 1156
BcoDI GTCTC 2 cut(s) 60, 577
BfaI CTAG 2 cut(s) 738, 1446
BfmI CTRYAG 1 cut(s) 867
BfuI GTATCC 2 cut(s) 134, 358
BglII AGATCT 1 cut(s) 863
BisI GCNGC 2 cut(s) 418, 1440
BlsI GCNGC 2 cut(s) 419, 1441
Bme1390I CCNGG 4 cut(s) 218, 390, 982, 1156
Bme18I GGWCC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 900
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 5 cut(s) 329, 458, 880, 979, 1377
BmrFI CCNGG 4 cut(s) 218, 390, 982, 1156
BmsI GCATC 1 cut(s) 21
BpiI GAAGAC 3 cut(s) 205, 945, 1250
BplI GAGNNNNNCTC 2 cut(s) 1117, 1149
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 2 cut(s) 540, 941
BpuMI CCSGG 2 cut(s) 982, 1156
BsaAI YACGTR 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 2 cut(s) 210, 805
BsaWI WCCGGW 1 cut(s) 453
Bsc4I CCNNNNNNNGG 1 cut(s) 1161
Bse1I ACTGG 2 cut(s) 831, 1107
Bse3DI GCAATG 1 cut(s) 1001
Bse8I GATNNNNATC 1 cut(s) 258
BseAI TCCGGA 1 cut(s) 453
BseBI CCWGG 2 cut(s) 218, 390
BseGI GGATG 1 cut(s) 620
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 1161
BseMI GCAATG 1 cut(s) 1001
BseMII CTCAG 4 cut(s) 579, 623, 698, 1043
BseNI ACTGG 2 cut(s) 831, 1107
BseRI GAGGAG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 1378
BseXI GCAGC 2 cut(s) 429, 1451
BsgI GTGCAG 2 cut(s) 801, 894
BshFI GGCC 2 cut(s) 770, 1229
BshNI GGYRCC 2 cut(s) 977, 1375
BsiHKCI CYCGRG 1 cut(s) 900
BsiSI CCGG 3 cut(s) 454, 981, 1156
BslFI GGGAC 5 cut(s) 341, 384, 563, 966, 1444
BslI CCNNNNNNNGG 1 cut(s) 1161
BsmAI GTCTC 2 cut(s) 60, 577
BsmBI CGTCTC 1 cut(s) 60
BsmFI GGGAC 5 cut(s) 341, 384, 563, 966, 1444
BsmI GAATGC 1 cut(s) 1193
BsnI GGCC 2 cut(s) 770, 1229
BsoBI CYCGRG 1 cut(s) 900
Bsp1286I GDGCHC 1 cut(s) 1378
Bsp13I TCCGGA 1 cut(s) 453
Bsp1407I TGTACA 3 cut(s) 443, 1074, 1429
Bsp143I GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
BspACI CCGC 1 cut(s) 745
BspANI GGCC 2 cut(s) 770, 1229
BspCNI CTCAG 4 cut(s) 580, 624, 697, 1042
BspEI TCCGGA 1 cut(s) 453
BspLI GGNNCC 5 cut(s) 329, 458, 880, 979, 1377
BspPI GGATC 6 cut(s) 96, 261, 509, 873, 886, 1113
BspT107I GGYRCC 2 cut(s) 977, 1375
BsrBI CCGCTC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 1001
BsrGI TGTACA 3 cut(s) 443, 1074, 1429
BsrI ACTGG 2 cut(s) 831, 1107
BssMI GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
BssNI GRCGYC 2 cut(s) 210, 805
Bst2UI CCWGG 2 cut(s) 218, 390
Bst4CI ACNGT 7 cut(s) 232, 354, 410, 977, 1079, 1414, 1516
Bst6I CTCTTC 4 cut(s) 341, 1028, 1061, 1154
BstACI GRCGYC 2 cut(s) 210, 805
BstAUI TGTACA 3 cut(s) 443, 1074, 1429
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 3 cut(s) 543, 929, 1017
BstDEI CTNAG 6 cut(s) 277, 588, 632, 684, 777, 1029
BstF5I GGATG 1 cut(s) 620
BstKTI GATC 9 cut(s) 91, 156, 256, 517, 676, 866, 881, 1108, 1503
BstMAI GTCTC 2 cut(s) 60, 577
BstMBI GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
BstMWI GCNNNNNNNGC 2 cut(s) 742, 991
BstNI CCWGG 2 cut(s) 218, 390
BstSCI CCNGG 4 cut(s) 216, 388, 980, 1154
BstSFI CTRYAG 1 cut(s) 867
BstSLI GKGCMC 1 cut(s) 1378
BstSNI TACGTA 1 cut(s) 887
BstV1I GCAGC 2 cut(s) 429, 1451
BstV2I GAAGAC 3 cut(s) 205, 945, 1250
BstX2I RGATCY 2 cut(s) 863, 878
BstYI RGATCY 2 cut(s) 863, 878
BsuI GTATCC 2 cut(s) 134, 358
BsuRI GGCC 2 cut(s) 770, 1229
BtsCI GGATG 1 cut(s) 620
BtsI GCAGTG 2 cut(s) 733, 1128
BtsIMutI CAGTG 6 cut(s) 201, 237, 415, 733, 1128, 1521
Cac8I GCNNGC 3 cut(s) 543, 929, 1017
Cfr13I GGNCC 1 cut(s) 328
CseI GACGC 1 cut(s) 813
Csp6I GTAC 5 cut(s) 444, 1025, 1075, 1430, 1465
CspCI CAANNNNNGTGG 2 cut(s) 1305, 1340
CviAII CATG 7 cut(s) 318, 461, 611, 772, 933, 1109, 1265
CviQI GTAC 5 cut(s) 444, 1025, 1075, 1430, 1465
DdeI CTNAG 6 cut(s) 277, 588, 632, 684, 777, 1029
DpnI GATC 9 cut(s) 90, 155, 255, 516, 675, 865, 880, 1107, 1502
DpnII GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 4 cut(s) 341, 1028, 1061, 1154
EarI CTCTTC 4 cut(s) 341, 1028, 1061, 1154
Eco105I TACGTA 1 cut(s) 887
Eco47I GGWCC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 1065
Eco88I CYCGRG 1 cut(s) 900
EcoO109I RGGNCCY 1 cut(s) 328
EcoRI GAATTC 2 cut(s) 1039, 1366
EcoRII CCWGG 2 cut(s) 216, 388
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 7 cut(s) 321, 464, 614, 775, 936, 1112, 1268
FalI AAGNNNNNCTT 2 cut(s) 1151, 1183
FaqI GGGAC 5 cut(s) 341, 384, 563, 966, 1444
FatI CATG 7 cut(s) 317, 460, 610, 771, 932, 1108, 1264
Fnu4HI GCNGC 2 cut(s) 418, 1440
FokI GGATG 1 cut(s) 627
Fsp4HI GCNGC 2 cut(s) 418, 1440
FspBI CTAG 2 cut(s) 738, 1446
GluI GCNGC 2 cut(s) 418, 1440
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 2 cut(s) 770, 1229
HapII CCGG 3 cut(s) 454, 981, 1156
HgaI GACGC 1 cut(s) 813
Hin1I GRCGYC 2 cut(s) 210, 805
Hin1II CATG 7 cut(s) 321, 464, 614, 775, 936, 1112, 1268
HincII GTYRAC 4 cut(s) 6, 436, 466, 1492
HindII GTYRAC 4 cut(s) 6, 436, 466, 1492
HindIII AAGCTT 3 cut(s) 539, 1307, 1452
HinfI GANTC 4 cut(s) 954, 1282, 1385, 1403
HpaI GTTAAC 2 cut(s) 466, 1492
HpaII CCGG 3 cut(s) 454, 981, 1156
HphI GGTGA 6 cut(s) 129, 305, 500, 604, 677, 1432
Hpy166II GTNNAC 6 cut(s) 6, 436, 444, 466, 1130, 1492
Hpy188I TCNGA 8 cut(s) 84, 184, 633, 699, 1032, 1045, 1222, 1390
Hpy188III TCNNGA 8 cut(s) 377, 454, 557, 801, 958, 1184, 1407, 1504
Hpy8I GTNNAC 6 cut(s) 6, 436, 444, 466, 1130, 1492
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 4 cut(s) 42, 127, 341, 591
HpyCH4III ACNGT 7 cut(s) 232, 354, 410, 977, 1079, 1414, 1516
HpyCH4IV ACGT 3 cut(s) 210, 886, 1494
HpyCH4V TGCA 9 cut(s) 428, 478, 726, 818, 875, 1019, 1121, 1142, 1439
HpyF10VI GCNNNNNNNGC 2 cut(s) 742, 991
HpyF3I CTNAG 6 cut(s) 277, 588, 632, 684, 777, 1029
HpySE526I ACGT 3 cut(s) 210, 886, 1494
Hsp92I GRCGYC 2 cut(s) 210, 805
Hsp92II CATG 7 cut(s) 321, 464, 614, 775, 936, 1112, 1268
Kpn2I TCCGGA 1 cut(s) 453
KspAI GTTAAC 2 cut(s) 466, 1492
Kzo9I GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
LmnI GCTCC 2 cut(s) 34, 456
Lsp1109I GCAGC 2 cut(s) 429, 1451
LweI GCATC 1 cut(s) 21
MaeI CTAG 2 cut(s) 738, 1446
MaeII ACGT 3 cut(s) 210, 886, 1494
MalI GATC 9 cut(s) 90, 155, 255, 516, 675, 865, 880, 1107, 1502
MbiI CCGCTC 1 cut(s) 745
MboI GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
MflI RGATCY 2 cut(s) 863, 878
MhlI GDGCHC 1 cut(s) 1378
MlsI TGGCCA 1 cut(s) 1229
MluCI AATT 9 cut(s) 185, 308, 627, 788, 809, 1039, 1256, 1277, 1366
MluNI TGGCCA 1 cut(s) 1229
MlyI GAGTC 2 cut(s) 948, 1412
Mox20I TGGCCA 1 cut(s) 1229
MroI TCCGGA 1 cut(s) 453
MroXI GAANNNNTTC 1 cut(s) 1260
MscI TGGCCA 1 cut(s) 1229
MseI TTAA 4 cut(s) 465, 1008, 1212, 1491
MslI CAYNNNNRTG 2 cut(s) 322, 613
Msp20I TGGCCA 1 cut(s) 1229
MspI CCGG 3 cut(s) 454, 981, 1156
MspR9I CCNGG 4 cut(s) 218, 390, 982, 1156
Mva1269I GAATGC 1 cut(s) 1193
MvaI CCWGG 2 cut(s) 218, 390
MwoI GCNNNNNNNGC 2 cut(s) 742, 991
NciI CCSGG 2 cut(s) 982, 1156
NdeII GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
NlaIII CATG 7 cut(s) 321, 464, 614, 775, 936, 1112, 1268
NlaIV GGNNCC 5 cut(s) 329, 458, 880, 979, 1377
NmeAIII GCCGAG 1 cut(s) 80
NmuCI GTSAC 6 cut(s) 16, 606, 855, 998, 1420, 1516
PctI GAATGC 1 cut(s) 1193
PdmI GAANNNNTTC 1 cut(s) 1260
PfeI GAWTC 2 cut(s) 1282, 1385
PflFI GACNNNGTC 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 2 cut(s) 419, 1441
PleI GAGTC 2 cut(s) 948, 1411
PpsI GAGTC 2 cut(s) 948, 1411
Ppu21I YACGTR 1 cut(s) 887
PpuMI RGGWCCY 1 cut(s) 328
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 216, 388
PspGI CCWGG 2 cut(s) 216, 388
PspN4I GGNNCC 5 cut(s) 329, 458, 880, 979, 1377
PspPI GGNCC 1 cut(s) 328
PspPPI RGGWCCY 1 cut(s) 328
PsuI RGATCY 2 cut(s) 863, 878
PsyI GACNNNGTC 1 cut(s) 209
RsaI GTAC 5 cut(s) 445, 1026, 1076, 1431, 1466
RsaNI GTAC 5 cut(s) 444, 1025, 1075, 1430, 1465
RseI CAYNNNNRTG 2 cut(s) 322, 613
SaqAI TTAA 4 cut(s) 465, 1008, 1212, 1491
SatI GCNGC 2 cut(s) 418, 1440
Sau3AI GATC 9 cut(s) 88, 153, 253, 514, 673, 863, 878, 1105, 1500
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 2 cut(s) 948, 1412
ScrFI CCNGG 4 cut(s) 218, 390, 982, 1156
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 1 cut(s) 21
SfcI CTRYAG 1 cut(s) 867
SinI GGWCC 1 cut(s) 328
SmiMI CAYNNNNRTG 2 cut(s) 322, 613
SmlI CTYRAG 2 cut(s) 555, 956
SmoI CTYRAG 2 cut(s) 555, 956
SnaBI TACGTA 1 cut(s) 887
Sse9I AATT 9 cut(s) 185, 308, 627, 788, 809, 1039, 1256, 1277, 1366
SsiI CCGC 1 cut(s) 745
SspI AATATT 1 cut(s) 1150
SspMI CTAG 2 cut(s) 738, 1446
StyD4I CCNGG 4 cut(s) 216, 388, 980, 1154
TaaI ACNGT 7 cut(s) 232, 354, 410, 977, 1079, 1414, 1516
TaiI ACGT 3 cut(s) 213, 889, 1497
TaqI TCGA 3 cut(s) 378, 676, 1296
TaqII GACCGA 1 cut(s) 1302
TasI AATT 9 cut(s) 185, 308, 627, 788, 809, 1039, 1256, 1277, 1366
TatI WGTACW 4 cut(s) 443, 1074, 1429, 1464
TfiI GAWTC 2 cut(s) 1282, 1385
Tru1I TTAA 4 cut(s) 465, 1008, 1212, 1491
Tru9I TTAA 4 cut(s) 465, 1008, 1212, 1491
TscAI CASTG 6 cut(s) 208, 237, 415, 733, 1128, 1521
TseFI GTSAC 6 cut(s) 16, 606, 855, 998, 1420, 1516
TseI GCWGC 2 cut(s) 417, 1439
Tsp45I GTSAC 6 cut(s) 16, 606, 855, 998, 1420, 1516
TspDTI ATGAA 4 cut(s) 396, 551, 639, 1253
TspGWI ACGGA 3 cut(s) 110, 160, 784
TspRI CASTG 6 cut(s) 208, 237, 415, 733, 1128, 1521
Tth111I GACNNNGTC 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 328
XapI RAATTY 5 cut(s) 185, 308, 627, 1039, 1366
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 1260
XspI CTAG 2 cut(s) 738, 1446
ZraI GACGTC 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.