Rh2DG699000

N-alpha-acetyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011920
Physical Location & Seq
Forward (+)
91418430 .. 91419343
914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG699000.1

Sequence Viewer

Length: 558 bp
ATGGAATTCCTTAGTAAGAAAGATGAAGAAGAAGCGCGGGCGGCGTCGGAGATAGAGTATGTGAGCTATGAGGGAGAAGATCATCTGCCGCTGATCATGGGGCTGGTGGACAAAGAGCTGAGCGAGCCATACTCGATCTTCACGTACAGGTACTTCGTGTACCTGTGGCCTCAACTGTGTTTTCTGGCATTCCACAGAGGCAAGTGTGTGGGAACCGTCGTCTGTAAGATGGGGGAACATCGCAACACCTACAGAGGCTACATAGCCATGCTCGTCGTCATCGATTCCTACAGAGGTAAAGGGATCGCTACTCAACTCGTTACCAGATCTATTCAAGTCATGATGGAATCAGGTTGCGAAGAGGTCACACTTGAGGCAGAAGTGACGAATAAAGGAGCACTGGCACTGTATGGGCGTCTTGGGTTCGTAAGGGCCAAAAGGCTCTTCCGTTACTACTTGAACGGCGTTGATGCTTTCCGTCTCAAGCTGCTATTTCCCCGCCCAGATTTGCATTCCCCGACTTCCCCTTCCCCTTCGCCTTATATGAGGCTCGATTGA

Protein Analysis

185

Amino Acids

21.28

Weight (kDa)

6.9

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 38 - 142 6.1e-21 Acetyltransferase (GNAT) family
Acetyltransf_7 PF13508 59 - 143 8.2e-12 Acetyltransferase (GNAT) domain
Acetyltransf_10 PF13673 59 - 149 2.1e-09 Acetyltransferase (GNAT) domain
FR47 PF08445 82 - 145 7.9e-07 FR47-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011920)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38130 AT2G38130
fragaria_vesca FvH4_6g54330
malus_domestica MD09G1002300.v1.1
prunus_persica Prupe.3G313900_v2.0.a1
pyrus_communis pycom111g00180 pycom17g00210
rosa_chinensis RchiOBHm_Chr2g0176551
rosa_laevigata RLG00000022422
rosa_multiflora Rmu_co8099318.1_g000001 Rmu_sc0001417.1_g000008 Rmu_sc0011904.1_g000003
rosa_roxburghii Rroxscaffold_2G00076310
rosa_rugosa Rorug02G0596100
rosa_samantha Rh2AG673900 Rh2BG685100 Rh2CG648100 Rh2DG699000
rosa_wichuraiana Rw2G055280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 37
AciI CCGC 4 cut(s) 37, 41, 89, 499
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 1 cut(s) 5
AcyI GRCGYC 2 cut(s) 44, 415
AfaI GTAC 3 cut(s) 146, 152, 161
AgsI TTSAA 2 cut(s) 335, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 3 cut(s) 66, 118, 487
AluI AGCT 3 cut(s) 66, 118, 487
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 1 cut(s) 311
AoxI GGCC 2 cut(s) 167, 432
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 1 cut(s) 5
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 1 cut(s) 432
BarI GAAGNNNNNNTAC 2 cut(s) 137, 169
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 474
BccI CCATC 2 cut(s) 223, 337
BceAI ACGGC 1 cut(s) 478
BclI TGATCA 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 485
BfmI CTRYAG 2 cut(s) 250, 289
BglII AGATCT 1 cut(s) 326
BisI GCNGC 3 cut(s) 42, 89, 488
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 3 cut(s) 43, 90, 489
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 1 cut(s) 214
BmsI GCATC 1 cut(s) 460
BplI GAGNNNNNCTC 2 cut(s) 116, 148
Bpu1102I GCTNAGC 1 cut(s) 119
BpuEI CTTGAG 2 cut(s) 392, 467
Bsa29I ATCGAT 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 144
BsaHI GRCGYC 2 cut(s) 44, 415
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bse1I ACTGG 1 cut(s) 405
BseCI ATCGAT 1 cut(s) 282
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 405
BseXI GCAGC 1 cut(s) 474
Bsh1236I CGCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 169, 434
BshVI ATCGAT 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 400
BsmAI GTCTC 1 cut(s) 485
BsmBI CGTCTC 1 cut(s) 485
BsmI GAATGC 2 cut(s) 188, 511
BsnI GGCC 2 cut(s) 169, 434
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 5 cut(s) 79, 93, 135, 303, 326
Bsp1720I GCTNAGC 1 cut(s) 119
BspACI CCGC 4 cut(s) 37, 41, 89, 499
BspANI GGCC 2 cut(s) 169, 434
BspCNI CTCAG 1 cut(s) 111
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 1 cut(s) 37
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 214
BspPI GGATC 1 cut(s) 311
BspQI GCTCTTC 1 cut(s) 449
BsrI ACTGG 1 cut(s) 405
BssMI GATC 5 cut(s) 79, 93, 135, 303, 326
BssNI GRCGYC 2 cut(s) 44, 415
Bst4CI ACNGT 3 cut(s) 177, 217, 408
Bst6I CTCTTC 2 cut(s) 354, 449
BstACI GRCGYC 2 cut(s) 44, 415
BstBAI YACGTR 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 39, 125
BstDEI CTNAG 2 cut(s) 11, 119
BstFNI CGCG 1 cut(s) 37
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 5 cut(s) 82, 96, 138, 306, 329
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 5 cut(s) 79, 93, 135, 303, 326
BstMWI GCNNNNNNNGC 2 cut(s) 41, 124
BstSFI CTRYAG 2 cut(s) 250, 289
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 1 cut(s) 474
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
Bsu15I ATCGAT 1 cut(s) 282
BsuRI GGCC 2 cut(s) 169, 434
BsuTUI ATCGAT 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 398, 404
Cac8I GCNNGC 2 cut(s) 39, 125
CciI TCATGA 1 cut(s) 339
CfoI GCGC 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 432
ClaI ATCGAT 1 cut(s) 282
CseI GACGC 2 cut(s) 33, 404
Csp6I GTAC 3 cut(s) 145, 151, 160
CviAII CATG 3 cut(s) 97, 268, 340
CviQI GTAC 3 cut(s) 145, 151, 160
DdeI CTNAG 2 cut(s) 11, 119
DpnI GATC 5 cut(s) 81, 95, 137, 305, 328
DpnII GATC 5 cut(s) 79, 93, 135, 303, 326
Eam1104I CTCTTC 2 cut(s) 354, 449
EarI CTCTTC 2 cut(s) 354, 449
EcoRI GAATTC 1 cut(s) 5
Esp3I CGTCTC 1 cut(s) 485
FaeI CATG 3 cut(s) 100, 271, 343
FatI CATG 3 cut(s) 96, 267, 339
FauI CCCGC 2 cut(s) 30, 506
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 42, 89, 488
Fsp4HI GCNGC 3 cut(s) 42, 89, 488
GlaI GCGC 1 cut(s) 36
GluI GCNGC 3 cut(s) 42, 89, 488
HaeIII GGCC 2 cut(s) 169, 434
HgaI GACGC 2 cut(s) 33, 404
HhaI GCGC 1 cut(s) 37
Hin1I GRCGYC 2 cut(s) 44, 415
Hin1II CATG 3 cut(s) 100, 271, 343
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HinfI GANTC 2 cut(s) 284, 347
Hpy166II GTNNAC 2 cut(s) 109, 160
Hpy188I TCNGA 1 cut(s) 49
Hpy188III TCNNGA 1 cut(s) 340
Hpy8I GTNNAC 2 cut(s) 109, 160
Hpy99I CGWCG 3 cut(s) 49, 221, 278
HpyAV CCTTC 2 cut(s) 537, 543
HpyCH4III ACNGT 3 cut(s) 177, 217, 408
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 1 cut(s) 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 124
HpyF3I CTNAG 2 cut(s) 11, 119
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 2 cut(s) 44, 415
Hsp92II CATG 3 cut(s) 100, 271, 343
HspAI GCGC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 5 cut(s) 79, 93, 135, 303, 326
LguI GCTCTTC 1 cut(s) 449
LmnI GCTCC 1 cut(s) 395
LpnPI CCDG 8 cut(s) 89, 133, 170, 176, 336, 337, 386, 516
Lsp1109I GCAGC 1 cut(s) 474
LweI GCATC 1 cut(s) 460
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 4 cut(s) 319, 364, 382, 449
MalI GATC 5 cut(s) 81, 95, 137, 305, 328
MboI GATC 5 cut(s) 79, 93, 135, 303, 326
MboII GAAGA 6 cut(s) 38, 41, 89, 130, 371, 436
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 8 cut(s) 64, 180, 191, 248, 287, 355, 367, 540
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 91
Mva1269I GAATGC 2 cut(s) 188, 511
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 2 cut(s) 41, 124
NdeII GATC 5 cut(s) 79, 93, 135, 303, 326
NlaIII CATG 3 cut(s) 100, 271, 343
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 2 cut(s) 364, 382
PagI TCATGA 1 cut(s) 339
PciSI GCTCTTC 1 cut(s) 449
PcsI WCGNNNNNNNCGW 2 cut(s) 140, 279
PctI GAATGC 2 cut(s) 188, 511
PfeI GAWTC 2 cut(s) 284, 347
PkrI GCNGC 3 cut(s) 43, 90, 489
Ppu21I YACGTR 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 432
PsuI RGATCY 1 cut(s) 326
RsaI GTAC 3 cut(s) 146, 152, 161
RsaNI GTAC 3 cut(s) 145, 151, 160
RseI CAYNNNNRTG 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 449
SatI GCNGC 3 cut(s) 42, 89, 488
Sau3AI GATC 5 cut(s) 79, 93, 135, 303, 326
Sau96I GGNCC 1 cut(s) 432
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 1 cut(s) 460
SfcI CTRYAG 2 cut(s) 250, 289
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 2 cut(s) 371, 482
SmoI CTYRAG 2 cut(s) 371, 482
Sse9I AATT 1 cut(s) 5
SsiI CCGC 4 cut(s) 37, 41, 89, 499
TaaI ACNGT 3 cut(s) 177, 217, 408
TaiI ACGT 1 cut(s) 146
TaqI TCGA 3 cut(s) 134, 282, 552
TasI AATT 1 cut(s) 5
TauI GCSGC 2 cut(s) 44, 91
TfiI GAWTC 2 cut(s) 284, 347
TscAI CASTG 2 cut(s) 405, 411
TseFI GTSAC 2 cut(s) 364, 382
TseI GCWGC 1 cut(s) 487
Tsp45I GTSAC 2 cut(s) 364, 382
TspDTI ATGAA 1 cut(s) 39
TspGWI ACGGA 2 cut(s) 437, 467
TspRI CASTG 2 cut(s) 405, 411
XapI RAATTY 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.