Rh3BG061000

ubiquitin-like protein ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0016449
Physical Location & Seq
Reverse (-)
4131886 .. 4136890
5005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG061000.1

Sequence Viewer

Length: 984 bp
ATGTGGTTCAGAAGCGGTGCTTTAAAACACAACATTGTGTGTAAAAGAAAGTGTTTTACAGGGTTATCAATGACTCCAAAGGAATATGCAGATACTTCAAAGGGCCTTCAACGCGACAAAACTGCCAATAACTACTGTCCTGAGTGTGGACTCAGTCTGTTGGAGTGTGCTATACGAAACCAGCAGACCGATATGATTGAGTTGTTGCTTAAGAATGGAGCTGAAATTTTTCGAGATGATGACATTGGAAGAGGCATTGCGACAATGGACGCCTTAGTTAAGCTTGGTTATAAAGAAGTTGAAGGCGAGTTTCTGTACACTTCAAGCTCTATGGGGAGCTTCAACGTTTGGGCTACCTTTTCAGTCAAAAATCGTGGGATTGCTATATTTTATGGTGGCAAGAGTCGAATGAGTGTCCAGGGCAAGGAACCAGATGTGTATTTTGCATGGGGCAAGGCTACTTTCAAGTGGGAAGGATCTGTGTTACAGATCGAAGATGGATTGGAGACTTCCTTGCTGGAACCAGCAGAACCTCCATATTTGGGTAGCAAAGAGATATTTAATTGGTTTTGCAACCAGTTAAATGAGGCTATTGGTTTGGGTTCTGTGGTGGATGATGGCTTTGATCTCTTTGATATAGAGATGGCACTCAAAGCTTCACTGGACCATTTTGGACAGCCATCTTCACAGCCTTCAATGGCTGTTGGTTTGGGTTCTGTGGTGGATGATGGCTTTGATCTCTCTGATATAGAGATGGCACTCAAAGCTTCATTGGACCATTTTGGAGAGCCATCTTTACAGCCTTCTGCACCACCTTTTCCCGAAGATGACTGCACCATCTGTTTTGATCATAGAGTTGACACTGCCTTTGTTCCCTGCGGTCATCTTATCTGCTCACCATGTGCAGAAAAGATTCAGCATCAGAACTTGCGGTGTCCCTTCTGCCGTGAGGTGGTGGAGAACTTTGCAATTCCTAGGATTTGA

Protein Analysis

327

Amino Acids

36.25

Weight (kDa)

4.81

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 276 - 321 3.2e-13 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 276 - 316 3e-06 Ring finger domain
zf-C3HC4 PF00097 278 - 315 6.4e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 278 - 319 7.5e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
AccII CGCG 1 cut(s) 114
AciI CCGC 3 cut(s) 15, 879, 931
AclI AACGTT 1 cut(s) 345
AclWI GGATC 1 cut(s) 484
AcsI RAATTY 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 270
AdeI CACNNNGTG 1 cut(s) 902
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 4 cut(s) 146, 424, 542, 952
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 7 cut(s) 99, 110, 302, 324, 343, 466, 696
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 6 cut(s) 221, 283, 327, 339, 656, 767
AluI AGCT 6 cut(s) 221, 283, 327, 339, 656, 767
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 484
AoxI GGCC 1 cut(s) 103
ApoI RAATTY 1 cut(s) 225
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
Asp700I GAANNNNTTC 2 cut(s) 228, 912
AspA2I CCTAGG 1 cut(s) 974
AspS9I GGNCC 3 cut(s) 103, 664, 775
AsuHPI GGTGA 1 cut(s) 888
AvaII GGWCC 2 cut(s) 664, 775
AvrII CCTAGG 1 cut(s) 974
BccI CCATC 8 cut(s) 491, 611, 637, 688, 722, 748, 799, 845
BceAI ACGGC 1 cut(s) 930
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 419
BclI TGATCA 1 cut(s) 847
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 975
BfrI CTTAAG 1 cut(s) 209
BlnI CCTAGG 1 cut(s) 974
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 2 cut(s) 664, 775
BmgT120I GGNCC 3 cut(s) 103, 664, 775
BmiI GGNNCC 2 cut(s) 429, 522
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 928
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 418, 974
Bsc4I CCNNNNNNNGG 4 cut(s) 146, 424, 542, 952
Bse1I ACTGG 2 cut(s) 577, 666
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 418, 974
BseGI GGATG 2 cut(s) 619, 730
BseLI CCNNNNNNNGG 4 cut(s) 146, 424, 542, 952
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 132, 166
BseNI ACTGG 2 cut(s) 577, 666
BsgI GTGCAG 3 cut(s) 792, 817, 924
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 105
BslFI GGGAC 1 cut(s) 921
BslI CCNNNNNNNGG 4 cut(s) 146, 424, 542, 952
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 921
BsnI GGCC 1 cut(s) 105
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 5 cut(s) 476, 489, 625, 736, 847
BspACI CCGC 3 cut(s) 15, 879, 931
BspANI GGCC 1 cut(s) 105
BspCNI CTCAG 2 cut(s) 133, 165
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 429, 522
BspPI GGATC 1 cut(s) 484
BspTI CTTAAG 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 255
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 2 cut(s) 577, 666
BssECI CCNNGG 2 cut(s) 418, 974
BssMI GATC 5 cut(s) 476, 489, 625, 736, 847
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 974
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 1 cut(s) 137
Bst6I CTCTTC 1 cut(s) 244
BstACI GRCGYC 1 cut(s) 270
BstAFI CTTAAG 1 cut(s) 209
BstAUI TGTACA 1 cut(s) 315
BstDEI CTNAG 3 cut(s) 141, 152, 274
BstF5I GGATG 2 cut(s) 619, 730
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 5 cut(s) 479, 492, 628, 739, 850
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 5 cut(s) 476, 489, 625, 736, 847
BstMWI GCNNNNNNNGC 3 cut(s) 111, 653, 764
BstNI CCWGG 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 417
BstUI CGCG 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 2 cut(s) 619, 730
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 2 cut(s) 659, 861
Cfr13I GGNCC 3 cut(s) 103, 664, 775
CseI GACGC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 316
CspCI CAANNNNNGTGG 2 cut(s) 355, 390
CviAII CATG 2 cut(s) 447, 900
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 3 cut(s) 141, 152, 274
DpnI GATC 5 cut(s) 478, 491, 627, 738, 849
DpnII GATC 5 cut(s) 476, 489, 625, 736, 847
DraI TTTAAA 1 cut(s) 24
DraIII CACNNNGTG 1 cut(s) 902
Eam1104I CTCTTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 974
Eco47I GGWCC 2 cut(s) 664, 775
EcoO109I RGGNCCY 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 974
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 2 cut(s) 450, 903
FalI AAGNNNNNCTT 1 cut(s) 36
FaqI GGGAC 1 cut(s) 921
FatI CATG 2 cut(s) 446, 899
FbaI TGATCA 1 cut(s) 847
FokI GGATG 2 cut(s) 626, 737
FspBI CTAG 1 cut(s) 975
HaeIII GGCC 1 cut(s) 105
HgaI GACGC 1 cut(s) 278
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 2 cut(s) 450, 903
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 3 cut(s) 281, 654, 765
HinfI GANTC 4 cut(s) 73, 150, 403, 913
HphI GGTGA 1 cut(s) 888
Hpy166II GTNNAC 3 cut(s) 149, 318, 859
Hpy188I TCNGA 3 cut(s) 11, 745, 924
Hpy188III TCNNGA 3 cut(s) 140, 233, 821
Hpy8I GTNNAC 3 cut(s) 149, 318, 859
HpyAV CCTTC 6 cut(s) 116, 296, 467, 702, 813, 949
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 7 cut(s) 89, 446, 573, 809, 834, 905, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 653, 764
HpyF3I CTNAG 3 cut(s) 141, 152, 274
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 2 cut(s) 450, 903
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 5 cut(s) 476, 489, 625, 736, 847
LmnI GCTCC 2 cut(s) 218, 336
LweI GCATC 1 cut(s) 928
MaeI CTAG 1 cut(s) 975
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 483
MalI GATC 5 cut(s) 478, 491, 627, 738, 849
MboI GATC 5 cut(s) 476, 489, 625, 736, 847
MboII GAAGA 4 cut(s) 261, 506, 675, 836
MflI RGATCY 1 cut(s) 476
MluCI AATT 3 cut(s) 225, 562, 969
MlyI GAGTC 3 cut(s) 67, 144, 412
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 4 cut(s) 245, 543, 580, 943
MroXI GAANNNNTTC 2 cut(s) 228, 912
MseI TTAA 5 cut(s) 23, 210, 279, 561, 581
MspCI CTTAAG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 419
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 3 cut(s) 111, 653, 764
NdeII GATC 5 cut(s) 476, 489, 625, 736, 847
NlaIII CATG 2 cut(s) 450, 903
NlaIV GGNNCC 2 cut(s) 429, 522
PdmI GAANNNNTTC 2 cut(s) 228, 912
PfeI GAWTC 1 cut(s) 913
PflFI GACNNNGTC 1 cut(s) 153
PleI GAGTC 3 cut(s) 67, 144, 411
PpsI GAGTC 3 cut(s) 67, 144, 411
PsiI TTATAA 1 cut(s) 291
Psp1406I AACGTT 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 417
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 429, 522
PspPI GGNCC 3 cut(s) 103, 664, 775
PsuI RGATCY 1 cut(s) 476
PsyI GACNNNGTC 1 cut(s) 153
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SaqAI TTAA 5 cut(s) 23, 210, 279, 561, 581
Sau3AI GATC 5 cut(s) 476, 489, 625, 736, 847
Sau96I GGNCC 3 cut(s) 103, 664, 775
SchI GAGTC 3 cut(s) 67, 144, 412
ScrFI CCNGG 1 cut(s) 419
SfaNI GCATC 1 cut(s) 928
SinI GGWCC 2 cut(s) 664, 775
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 3 cut(s) 225, 562, 969
SsiI CCGC 3 cut(s) 15, 879, 931
SspMI CTAG 1 cut(s) 975
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 1 cut(s) 137
TaiI ACGT 1 cut(s) 348
TaqI TCGA 3 cut(s) 232, 406, 492
TaqII GACCGA 1 cut(s) 203
TasI AATT 3 cut(s) 225, 562, 969
TatI WGTACW 1 cut(s) 315
TfiI GAWTC 1 cut(s) 913
Tru1I TTAA 5 cut(s) 23, 210, 279, 561, 581
Tru9I TTAA 5 cut(s) 23, 210, 279, 561, 581
TscAI CASTG 2 cut(s) 666, 868
TspDTI ATGAA 1 cut(s) 759
TspRI CASTG 2 cut(s) 666, 868
Tth111I GACNNNGTC 1 cut(s) 153
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 664, 775
XapI RAATTY 1 cut(s) 225
XmaJI CCTAGG 1 cut(s) 974
XmnI GAANNNNTTC 2 cut(s) 228, 912
XspI CTAG 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.