Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0014448

Physical Location & Seq

Genomic Coordinates Forward (+)

21216289 .. 21220065

Gene Structure 3777 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG225200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2562 bp

ATGAGAGCAGGAGGTTGCACGCTCCAACAGGGTCTAACTACAGAGGCTGCGAACATAGTAAAGCTAGCACTAACCCTAGCAAGGCACCGAGGCCATGCCCAAGTCACTCCTCTCCATGTTGCAAACACCATGCTCTCTTCTTCAACCGGCCTATTGCGAACTGCTTGCCTTCAATCACACTCTCATCCACTTCAATGCAAAGCACTTGAGCTTTGCTTCAATGTTGCCCTCAACCGCCTCCCGGCCTCCAATTCCAGCCCCATGTTGGGTGGCGGTCACCATCCACAAAACCCTTCCATCTCCAACGCCTTGGTCGCTGCTTTCAAGCGCGCGCAGGCTCACCAACGCCGCGGTTCCATTGAGAACCAGCAGCAGCCCCTTCTAGCAGTGAAGATAGAGCTGGAGCAACTCATAATTTCAATCTTAGATGATCCAAGTGTAAGTAGAGTCATGAGAGAAGCTGGGTTTTCTAGTACCCAGGTCAAAAGCAATGTAGAACAAGCTGTCTCCATAGAAATATGTAACTCCCAAACTACTACTACTCCTTGTGTAAGTAGTAGTAAGTCCAAGGAGAGTAATCTCCAGTTGATTCCTTTGTCAGTGGTACCTAATCTAGATCCAGTTAGGAATGAGGATGTGACAAGTGTTATAGAGAATTTGGTGAAGAAAAGAAGGAAGAGTATTGTGATTGTGGGTGAGTGTCTTGCTAGTGTTGAGGGCGTGGTGAGGGGAGTGATGGAGAAGGTTGATAAGGGAGATCAACATGTTGTCGTTGATGAGGGTTTCAGTTTGAGAGAGGTGAAATTTGTAACACTTTCTCTCTCCTCTTTTGGACATATTTCTAGAGTAGAGGTTGAACAGAAACTTGGAGAACTCAAGAGCCTAGTGAGGAGCAGTGTGGGGAAAGGAATAATTTTGTATCTGGGAGATCTCAAGTGGATTACTGAGTATAGGACTAGTAGTAGTTCAAGTCATGATCAGCAGGGAATTAGGGGGTATTATTGTCCTGTGGAGCACATGATCATGGAGCTTGGGAACCTGGTTTGTGGGATTAATGGTGAGACTAATGGTGGGAGGGTTTGGCTTGTGGGGATTGCTACTTTTCAAACTTACATGAGATGCAAATCTGGCCATCCATCACTTGAGACTGTTTGGGGTATTCATCCTCTAACAATTCCCCCAACCAGCCTGCGCTTGAGTCTTGTCACAGCTGAAAGTGAGCTACACAGTGAGTCTACAAGCAAGATAGCTGAAACTGGAACTAGCAGGCTAGTAGGACTCGAAGGTGGATATCAGAAGAAGCTCACTTGCTGTGACGAATGCTCAAACAAGTTCGAAGAAGAAGCTCAAAGCTTACGAAGCAGTAGCATTTGTAACAGTGAATCCACCACTTCAAGCCTTCCTGCATGGCTCCAACAGTACAAAAATGAGAACAAAGGACTAAACACAACTAATAATAATCTTCAGAACTCTGTCTCAGTTTCAGACCTTTGCAAAAAGTGGAACTCCATTTGCAAAGCAATGCACCACCAACAGCATTCCAATAATTCTTCTGAGCTCAAGACTCTTACAATATTCTCTTCTCTCTCACCCTCTTCCTCCACTTCCGGTTTTTCATATGAGCAACAACAATACCCTACTTTGCACCACCATCATTCTTGGAGGGACCAACATTTCTGGATATCCGAAGCTCGTGACAAGGCTGTCGAACCCAATTTGACAATGTATTCATCAAATCCTAATTCCACTCCTAATTCGGCTTCATCTAGTGATGTCATGGAAACAGACTATGTTCCGAGGTTCAAGGAGATCAATGGCGAGAACCTGAAAACCCTATGCAATGCATTGGAGACAAAAGTGCCGTGGCAGAAGGATGTAATTCCTGACATAGCCTCCACAATCTTGAAATGCAGGTCCGGAATGGTCAGAAGAAAAGGGAGTAAGGTGGGCACTAATTTCAGCCCCGAGGTAAAAGAGGAAACATGGTTGTTCTTTCAAGGTCTTGATATGGAAGCTAAGGAAAAAGTTGCAAGGGAATTAGCTCGTCTTGTTTTCGGGTCTCAAACCAACCTCATTTCCATTGCACTAAGCAGTTTCTCGTCGACTCGGGCAGACTCAACCGAGGATTGCAGAAGCAAAAGACCACGAGACGAACAAAGTTGTAGTTATGTGGAAAGATTCGCTGAGGCGGTGGCGTTTAATCCACATAGGGTGTTTTTAGTTGAAGATGTAGAGCAAGCGGACTATTGCTCTCAAATGGGGTTTAAGAGAGCGATCGAAAGAGGGAGAATAACAAATTCGAGTGGAGAAGAAGTTGGCCTTGGAGATGCCATCATTATTTTGAGCTGCGAAAGCTTTAGTTCGAGATCAAGAGCTTGCTCTCCCCCGATAAAGCAAAAATCATCAGAAGACGAAGATCATAAGGATTCAAGTCCTCGTGCATCTTTGGATTTGAACATTTCGTTTGATAATGACGATGTTGATGGTGATCAGTCAATTGATGACATTGGTCTTCTTGAATCGGTTGATAGAAGGATCATTTTCAAAATTCAAGAACTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

Clp_N

Protein Analysis

853

Amino Acids

94.15

Weight (kDa)

6.36

Isoelectric Point (pI)

49.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	12 - 116	2.3e-06	Clp repeat (R) N-terminal domain
NBD_SMAX1	PF23569	210 - 403	7.3e-65	SMAX1 nucleotide binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52490
fragaria_vesca	FvH4_6g20090
malus_domestica	MD12G1037900.v1.1 MD14G1036900.v1.1
prunus_persica	Prupe.7G097400_v2.0.a1
pyrus_communis	pycom12g03280 pycom14g03230
rosa_chinensis	RchiOBHm_Chr3g0475541
rosa_laevigata	RLG00000023834
rosa_multiflora	Rmu_sc0000539.1_g000022
rosa_roxburghii	Rroxscaffold_6G00406190
rosa_rugosa	Rorug03G0150400
rosa_samantha	Rh3AG199900 Rh3BG230100 Rh3CG224600 Rh3DG225200
rosa_wichuraiana	Rw3G018130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1703
Acc36I	ACCTGC	1 cut(s)	1902
Acc65I	GGTACC	1 cut(s)	604
AccB1I	GGYRCC	2 cut(s)	84, 604
AccI	GTMKAC	2 cut(s)	1235, 2102
AccII	CGCG	3 cut(s)	330, 332, 351
AccIII	TCCGGA	1 cut(s)	1916
AciI	CCGC	6 cut(s)	235, 273, 349, 351, 2189, 2240
AclWI	GGATC	3 cut(s)	425, 611, 2543
AcoI	YGGCCR	1 cut(s)	1129
AcsI	RAATTY	4 cut(s)	655, 803, 2296, 2547
AcuI	CTGAAG	1 cut(s)	1448
AfaI	GTAC	3 cut(s)	475, 606, 1421
AfiI	CCNNNNNNNGG	4 cut(s)	81, 241, 265, 266
AflIII	ACRYGT	1 cut(s)	763
AhlI	ACTAGT	1 cut(s)	956
AjnI	CCWGG	2 cut(s)	477, 1038
AjuI	GAANNNNNNNTTGG	4 cut(s)	849, 881, 2304, 2336
Alw21I	GWGCWC	2 cut(s)	1017, 1560
Alw26I	GTCTC	7 cut(s)	511, 1055, 1139, 1480, 1844, 2064, 2142
AlwI	GGATC	3 cut(s)	425, 611, 2543
AlwNI	CAGNNNCTG	1 cut(s)	47
Ama87I	CYCGRG	2 cut(s)	1964, 2106
Aor13HI	TCCGGA	1 cut(s)	1916
AoxI	GGCC	5 cut(s)	91, 148, 243, 1129, 2317
ApeKI	GCWGC	5 cut(s)	47, 317, 370, 373, 2346
ApoI	RAATTY	4 cut(s)	655, 803, 2296, 2547
AseI	ATTAAT	1 cut(s)	1053
Asp718I	GGTACC	1 cut(s)	604
AspLEI	GCGC	4 cut(s)	330, 332, 334, 1194
AspS9I	GGNCC	2 cut(s)	1666, 1914
AsuC2I	CCSGG	1 cut(s)	242
AsuHPI	GGTGA	9 cut(s)	269, 332, 673, 707, 736, 811, 1070, 1581, 2498
AsuII	TTCGAA	1 cut(s)	1335
AsuNHI	GCTAGC	1 cut(s)	64
AvaI	CYCGRG	2 cut(s)	1964, 2106
AvaII	GGWCC	2 cut(s)	1666, 1914
BaeGI	GKGCMC	1 cut(s)	1952
BalI	TGGCCA	1 cut(s)	1131
BanI	GGYRCC	2 cut(s)	84, 604
BanII	GRGCYC	1 cut(s)	1560
BauI	CACGAG	3 cut(s)	1692, 2145, 2436
BbsI	GAAGAC	2 cut(s)	2415, 2504
Bbv12I	GWGCWC	2 cut(s)	1017, 1560
BbvCI	CCTCAGC	1 cut(s)	2184
BbvI	GCAGC	5 cut(s)	34, 304, 382, 385, 2333
BccI	CCATC	8 cut(s)	288, 305, 730, 1140, 1144, 1659, 2339, 2477
BceAI	ACGGC	1 cut(s)	1846
BcgI	CGANNNNNNTGC	2 cut(s)	147, 181
BciT130I	CCWGG	2 cut(s)	479, 1040
BclI	TGATCA	3 cut(s)	976, 1020, 2488
BcnI	CCSGG	1 cut(s)	242
BcoDI	GTCTC	7 cut(s)	511, 1055, 1139, 1480, 1844, 2064, 2142
BcuI	ACTAGT	1 cut(s)	956
BfmI	CTRYAG	1 cut(s)	39
BfuAI	ACCTGC	1 cut(s)	1902
BglII	AGATCT	1 cut(s)	928
BisI	GCNGC	6 cut(s)	48, 318, 349, 371, 374, 2347
BlsI	GCNGC	6 cut(s)	49, 319, 350, 372, 375, 2348
Bme1390I	CCNGG	3 cut(s)	242, 479, 1040
Bme18I	GGWCC	2 cut(s)	1666, 1914
BmeT110I	CYCGRG	2 cut(s)	1964, 2106
BmgT120I	GGNCC	2 cut(s)	1666, 1914
BmiI	GGNNCC	6 cut(s)	86, 355, 606, 1037, 1412, 1667
BmrFI	CCNGG	3 cut(s)	242, 479, 1040
BmsI	GCATC	3 cut(s)	1109, 2317, 2450
BmtI	GCTAGC	1 cut(s)	68
BoxI	GACNNNNGTC	1 cut(s)	2508
BpiI	GAAGAC	2 cut(s)	2415, 2504
BpmI	CTGGAG	2 cut(s)	422, 566
Bpu10I	CCTNAGC	2 cut(s)	2016, 2184
Bpu14I	TTCGAA	1 cut(s)	1335
BpuEI	CTTGAG	6 cut(s)	227, 860, 917, 1163, 1216, 1544
BpuMI	CCSGG	1 cut(s)	242
BsaBI	GATNNNNATC	2 cut(s)	1123, 2487
BsaI	GGTCTC	1 cut(s)	2064
BsaWI	WCCGGW	2 cut(s)	1607, 1916
BsaXI	ACNNNNNCTCC	6 cut(s)	526, 556, 1877, 1907, 2298, 2328
Bsc4I	CCNNNNNNNGG	4 cut(s)	81, 241, 265, 266
Bse118I	RCCGGY	1 cut(s)	146
Bse1I	ACTGG	3 cut(s)	583, 620, 1261
Bse3DI	GCAATG	4 cut(s)	496, 1527, 1846, 2079
Bse8I	GATNNNNATC	2 cut(s)	1123, 2487
BseAI	TCCGGA	1 cut(s)	1916
BseBI	CCWGG	2 cut(s)	479, 1040
BseGI	GGATG	6 cut(s)	184, 280, 640, 1132, 1162, 1879
BseJI	GATNNNNATC	2 cut(s)	1123, 2487
BseLI	CCNNNNNNNGG	4 cut(s)	81, 241, 265, 266
BseMI	GCAATG	4 cut(s)	496, 1527, 1846, 2079
BseMII	CTCAG	4 cut(s)	936, 1491, 1545, 2175
BseNI	ACTGG	3 cut(s)	583, 620, 1261
BsePI	GCGCGC	2 cut(s)	328, 330
BseRI	GAGGAG	3 cut(s)	99, 814, 904
BseSI	GKGCMC	1 cut(s)	1952
BseXI	GCAGC	5 cut(s)	34, 304, 382, 385, 2333
BseYI	CCCAGC	1 cut(s)	461
Bsh1236I	CGCG	3 cut(s)	330, 332, 351
Bsh1285I	CGRYCG	1 cut(s)	2277
BshFI	GGCC	5 cut(s)	93, 150, 245, 1131, 2319
BshNI	GGYRCC	2 cut(s)	84, 604
BsiEI	CGRYCG	1 cut(s)	2277
BsiHKAI	GWGCWC	2 cut(s)	1017, 1560
BsiHKCI	CYCGRG	2 cut(s)	1964, 2106
BsiSI	CCGG	4 cut(s)	147, 242, 1608, 1917
BslFI	GGGAC	1 cut(s)	1679
BslI	CCNNNNNNNGG	4 cut(s)	81, 241, 265, 266
BsmAI	GTCTC	7 cut(s)	511, 1055, 1139, 1480, 1844, 2064, 2142
BsmBI	CGTCTC	1 cut(s)	2142
BsmFI	GGGAC	1 cut(s)	1679
BsmI	GAATGC	2 cut(s)	1325, 1537
BsnI	GGCC	5 cut(s)	93, 150, 245, 1131, 2319
Bso31I	GGTCTC	1 cut(s)	2064
BsoBI	CYCGRG	2 cut(s)	1964, 2106
Bsp119I	TTCGAA	1 cut(s)	1335
Bsp1286I	GDGCHC	3 cut(s)	1017, 1560, 1952
Bsp13I	TCCGGA	1 cut(s)	1916
BspACI	CCGC	6 cut(s)	235, 273, 349, 351, 2189, 2240
BspANI	GGCC	5 cut(s)	93, 150, 245, 1131, 2319
BspCNI	CTCAG	4 cut(s)	937, 1490, 1546, 2176
BspEI	TCCGGA	1 cut(s)	1916
BspFNI	CGCG	3 cut(s)	330, 332, 351
BspHI	TCATGA	2 cut(s)	450, 973
BspLI	GGNNCC	6 cut(s)	86, 355, 606, 1037, 1412, 1667
BspMI	ACCTGC	1 cut(s)	1902
BspOI	GCTAGC	1 cut(s)	68
BspPI	GGATC	3 cut(s)	425, 611, 2543
BspT104I	TTCGAA	1 cut(s)	1335
BspT107I	GGYRCC	2 cut(s)	84, 604
BspTNI	GGTCTC	1 cut(s)	2064
BsrDI	GCAATG	4 cut(s)	496, 1527, 1846, 2079
BsrFI	RCCGGY	1 cut(s)	146
BsrI	ACTGG	3 cut(s)	583, 620, 1261
BssAI	RCCGGY	1 cut(s)	146
BssHII	GCGCGC	2 cut(s)	328, 330
BssSI	CACGAG	3 cut(s)	1692, 2145, 2436
BssT1I	CCWWGG	3 cut(s)	309, 567, 2320
Bst2BI	CACGAG	3 cut(s)	1692, 2145, 2436
Bst2UI	CCWGG	2 cut(s)	479, 1040
Bst4CI	ACNGT	5 cut(s)	1150, 1229, 1379, 1419, 2559
Bst6I	CTCTTC	4 cut(s)	142, 671, 1585, 1600
BstBI	TTCGAA	1 cut(s)	1335
BstDEI	CTNAG	7 cut(s)	424, 945, 1477, 1554, 2016, 2087, 2184
BstDSI	CCRYGG	2 cut(s)	349, 1862
BstEII	GGTNACC	1 cut(s)	275
BstF5I	GGATG	6 cut(s)	184, 280, 640, 1132, 1162, 1879
BstFNI	CGCG	3 cut(s)	330, 332, 351
BstHHI	GCGC	4 cut(s)	330, 332, 334, 1194
BstMAI	GTCTC	7 cut(s)	511, 1055, 1139, 1480, 1844, 2064, 2142
BstMCI	CGRYCG	1 cut(s)	2277
BstMWI	GCNNNNNNNGC	4 cut(s)	314, 1128, 1359, 2352
BstNI	CCWGG	2 cut(s)	479, 1040
BstNSI	RCATGY	1 cut(s)	767
BstPAI	GACNNNNGTC	1 cut(s)	2508
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	3 cut(s)	240, 477, 1038
BstSFI	CTRYAG	1 cut(s)	39
BstSLI	GKGCMC	1 cut(s)	1952
BstUI	CGCG	3 cut(s)	330, 332, 351
BstV1I	GCAGC	5 cut(s)	34, 304, 382, 385, 2333
BstV2I	GAAGAC	2 cut(s)	2415, 2504
BstX2I	RGATCY	2 cut(s)	616, 928
BstXI	CCANNNNNNTGG	1 cut(s)	310
BstYI	RGATCY	2 cut(s)	616, 928
BsuRI	GGCC	5 cut(s)	93, 150, 245, 1131, 2319
BtgI	CCRYGG	2 cut(s)	349, 1862
BtsCI	GGATG	6 cut(s)	184, 280, 640, 1132, 1162, 1879
BtsI	GCAGTG	2 cut(s)	393, 901
BtsIMutI	CAGTG	5 cut(s)	393, 606, 901, 1234, 1384
BveI	ACCTGC	1 cut(s)	1902
CaiI	CAGNNNCTG	1 cut(s)	47
CciI	TCATGA	2 cut(s)	450, 973
CfoI	GCGC	4 cut(s)	330, 332, 334, 1194
Cfr10I	RCCGGY	1 cut(s)	146
Cfr13I	GGNCC	2 cut(s)	1666, 1914
Cfr42I	CCGCGG	1 cut(s)	352
CsiI	ACCWGGT	1 cut(s)	1038
Csp6I	GTAC	3 cut(s)	474, 605, 1420
CviQI	GTAC	3 cut(s)	474, 605, 1420
DdeI	CTNAG	7 cut(s)	424, 945, 1477, 1554, 2016, 2087, 2184
DrdI	GACNNNNNNGTC	1 cut(s)	1703
DseDI	GACNNNNNNGTC	1 cut(s)	1703
EaeI	YGGCCR	1 cut(s)	1129
Eam1104I	CTCTTC	4 cut(s)	142, 671, 1585, 1600
EarI	CTCTTC	4 cut(s)	142, 671, 1585, 1600
Ecl136II	GAGCTC	1 cut(s)	1558
Eco130I	CCWWGG	3 cut(s)	309, 567, 2320
Eco24I	GRGCYC	1 cut(s)	1560
Eco31I	GGTCTC	1 cut(s)	2064
Eco32I	GATATC	2 cut(s)	1292, 1683
Eco47I	GGWCC	2 cut(s)	1666, 1914
Eco53kI	GAGCTC	1 cut(s)	1558
Eco57I	CTGAAG	1 cut(s)	1448
Eco88I	CYCGRG	2 cut(s)	1964, 2106
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1558
EcoO65I	GGTNACC	1 cut(s)	275
EcoRII	CCWGG	2 cut(s)	477, 1038
EcoRV	GATATC	2 cut(s)	1292, 1683
EcoT14I	CCWWGG	3 cut(s)	309, 567, 2320
EcoT22I	ATGCAT	1 cut(s)	1846
EcoT38I	GRGCYC	1 cut(s)	1560
ErhI	CCWWGG	3 cut(s)	309, 567, 2320
Esp3I	CGTCTC	1 cut(s)	2142
FalI	AAGNNNNNCTT	2 cut(s)	2304, 2336
FaqI	GGGAC	1 cut(s)	1679
FauNDI	CATATG	1 cut(s)	1618
FbaI	TGATCA	3 cut(s)	976, 1020, 2488
FblI	GTMKAC	2 cut(s)	1235, 2102
Fnu4HI	GCNGC	6 cut(s)	48, 318, 349, 371, 374, 2347
FokI	GGATG	6 cut(s)	171, 267, 647, 1119, 1149, 1886
FriOI	GRGCYC	1 cut(s)	1560
Fsp4HI	GCNGC	6 cut(s)	48, 318, 349, 371, 374, 2347
GlaI	GCGC	4 cut(s)	329, 331, 333, 1193
GluI	GCNGC	6 cut(s)	48, 318, 349, 371, 374, 2347
GsaI	CCCAGC	1 cut(s)	465
GsuI	CTGGAG	2 cut(s)	422, 566
HaeIII	GGCC	5 cut(s)	93, 150, 245, 1131, 2319
HapII	CCGG	4 cut(s)	147, 242, 1608, 1917
HhaI	GCGC	4 cut(s)	330, 332, 334, 1194
Hin6I	GCGC	4 cut(s)	328, 330, 332, 1192
HinP1I	GCGC	4 cut(s)	328, 330, 332, 1192
HincII	GTYRAC	1 cut(s)	2103
HindII	GTYRAC	1 cut(s)	2103
HindIII	AAGCTT	2 cut(s)	1351, 2353
HpaII	CCGG	4 cut(s)	147, 242, 1608, 1917
HphI	GGTGA	9 cut(s)	269, 332, 673, 707, 736, 811, 1070, 1581, 2498
Hpy166II	GTNNAC	2 cut(s)	1236, 2103
Hpy188I	TCNGA	8 cut(s)	1296, 1467, 1486, 1555, 1687, 1797, 1928, 2407
Hpy8I	GTNNAC	2 cut(s)	1236, 2103
Hpy99I	CGWCG	1 cut(s)	2104
HpyAV	CCTTC	9 cut(s)	179, 303, 389, 666, 736, 1277, 1409, 1864, 2526
HpyCH4III	ACNGT	5 cut(s)	1150, 1229, 1379, 1419, 2559
HpyF10VI	GCNNNNNNNGC	4 cut(s)	314, 1128, 1359, 2352
HpyF3I	CTNAG	7 cut(s)	424, 945, 1477, 1554, 2016, 2087, 2184
HspAI	GCGC	4 cut(s)	328, 330, 332, 1192
Kpn2I	TCCGGA	1 cut(s)	1916
KpnI	GGTACC	1 cut(s)	608
Ksp22I	TGATCA	3 cut(s)	976, 1020, 2488
KspI	CCGCGG	1 cut(s)	352
LmnI	GCTCC	6 cut(s)	27, 403, 891, 1012, 1027, 1416
Lsp1109I	GCAGC	5 cut(s)	34, 304, 382, 385, 2333
LweI	GCATC	3 cut(s)	1109, 2317, 2450
MabI	ACCWGGT	1 cut(s)	1038
MaeIII	GTNAC	9 cut(s)	103, 275, 521, 637, 808, 1204, 1313, 1373, 1694
MfeI	CAATTG	1 cut(s)	2496
MflI	RGATCY	2 cut(s)	616, 928
MhlI	GDGCHC	3 cut(s)	1017, 1560, 1952
MlsI	TGGCCA	1 cut(s)	1131
MluNI	TGGCCA	1 cut(s)	1131
MlyI	GAGTC	7 cut(s)	456, 1207, 1241, 1272, 1558, 2098, 2108
MmeI	TCCRAC	3 cut(s)	49, 327, 1438
Mox20I	TGGCCA	1 cut(s)	1131
Mph1103I	ATGCAT	1 cut(s)	1846
MroI	TCCGGA	1 cut(s)	1916
MscI	TGGCCA	1 cut(s)	1131
MseI	TTAA	3 cut(s)	1053, 2199, 2265
MslI	CAYNNNNRTG	2 cut(s)	193, 1022
Msp20I	TGGCCA	1 cut(s)	1131
MspA1I	CMGCKG	2 cut(s)	351, 1211
MspI	CCGG	4 cut(s)	147, 242, 1608, 1917
MspR9I	CCNGG	3 cut(s)	242, 479, 1040
MunI	CAATTG	1 cut(s)	2496
Mva1269I	GAATGC	2 cut(s)	1325, 1537
MvaI	CCWGG	2 cut(s)	479, 1040
MvnI	CGCG	3 cut(s)	330, 332, 351
MwoI	GCNNNNNNNGC	4 cut(s)	314, 1128, 1359, 2352
NciI	CCSGG	1 cut(s)	242
NdeI	CATATG	1 cut(s)	1618
NheI	GCTAGC	1 cut(s)	64
NlaIV	GGNNCC	6 cut(s)	86, 355, 606, 1037, 1412, 1667
NmuCI	GTSAC	6 cut(s)	103, 275, 637, 1204, 1313, 1694
NsiI	ATGCAT	1 cut(s)	1846
NspI	RCATGY	1 cut(s)	767
NspV	TTCGAA	1 cut(s)	1335
PagI	TCATGA	2 cut(s)	450, 973
PauI	GCGCGC	2 cut(s)	328, 330
PciI	ACATGT	1 cut(s)	763
PctI	GAATGC	2 cut(s)	1325, 1537
PfeI	GAWTC	5 cut(s)	589, 1382, 2178, 2426, 2519
PkrI	GCNGC	6 cut(s)	49, 319, 350, 372, 375, 2348
Ple19I	CGATCG	1 cut(s)	2277
PleI	GAGTC	7 cut(s)	455, 1206, 1240, 1272, 1558, 2098, 2108
PpsI	GAGTC	7 cut(s)	455, 1206, 1240, 1272, 1558, 2098, 2108
PscI	ACATGT	1 cut(s)	763
PshAI	GACNNNNGTC	1 cut(s)	2508
PshBI	ATTAAT	1 cut(s)	1053
Psp124BI	GAGCTC	1 cut(s)	1560
Psp6I	CCWGG	2 cut(s)	477, 1038
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	1 cut(s)	461
PspGI	CCWGG	2 cut(s)	477, 1038
PspN4I	GGNNCC	6 cut(s)	86, 355, 606, 1037, 1412, 1667
PspPI	GGNCC	2 cut(s)	1666, 1914
PstNI	CAGNNNCTG	1 cut(s)	47
PsuI	RGATCY	2 cut(s)	616, 928
PteI	GCGCGC	2 cut(s)	328, 330
PvuI	CGATCG	1 cut(s)	2277
PvuII	CAGCTG	1 cut(s)	1211
RsaI	GTAC	3 cut(s)	475, 606, 1421
RsaNI	GTAC	3 cut(s)	474, 605, 1420
RseI	CAYNNNNRTG	2 cut(s)	193, 1022
SacI	GAGCTC	1 cut(s)	1560
SacII	CCGCGG	1 cut(s)	352
SalI	GTCGAC	1 cut(s)	2101
SaqAI	TTAA	3 cut(s)	1053, 2199, 2265
SatI	GCNGC	6 cut(s)	48, 318, 349, 371, 374, 2347
Sau96I	GGNCC	2 cut(s)	1666, 1914
SchI	GAGTC	7 cut(s)	456, 1207, 1241, 1272, 1558, 2098, 2108
ScrFI	CCNGG	3 cut(s)	242, 479, 1040
SduI	GDGCHC	3 cut(s)	1017, 1560, 1952
SexAI	ACCWGGT	1 cut(s)	1038
SfaNI	GCATC	3 cut(s)	1109, 2317, 2450
SfcI	CTRYAG	1 cut(s)	39
Sfr303I	CCGCGG	1 cut(s)	352
SfuI	TTCGAA	1 cut(s)	1335
SgrBI	CCGCGG	1 cut(s)	352
SinI	GGWCC	2 cut(s)	1666, 1914
SmiMI	CAYNNNNRTG	2 cut(s)	193, 1022
SmlI	CTYRAG	6 cut(s)	206, 875, 932, 1142, 1195, 1559
SmoI	CTYRAG	6 cut(s)	206, 875, 932, 1142, 1195, 1559
SpeI	ACTAGT	1 cut(s)	956
SsiI	CCGC	6 cut(s)	235, 273, 349, 351, 2189, 2240
SspI	AATATT	1 cut(s)	1575
SstI	GAGCTC	1 cut(s)	1560
StyD4I	CCNGG	3 cut(s)	240, 477, 1038
StyI	CCWWGG	3 cut(s)	309, 567, 2320
TaaI	ACNGT	5 cut(s)	1150, 1229, 1379, 1419, 2559
TaqI	TCGA	7 cut(s)	1281, 1335, 1707, 2102, 2277, 2300, 2363
TatI	WGTACW	1 cut(s)	1419
TauI	GCSGC	1 cut(s)	351
TfiI	GAWTC	5 cut(s)	589, 1382, 2178, 2426, 2519
Tru1I	TTAA	3 cut(s)	1053, 2199, 2265
Tru9I	TTAA	3 cut(s)	1053, 2199, 2265
TscAI	CASTG	5 cut(s)	393, 606, 901, 1234, 1384
TseFI	GTSAC	6 cut(s)	103, 275, 637, 1204, 1313, 1694
TseI	GCWGC	5 cut(s)	47, 317, 370, 373, 2346
Tsp45I	GTSAC	6 cut(s)	103, 275, 637, 1204, 1313, 1694
TspDTI	ATGAA	4 cut(s)	1151, 1605, 1719, 1752
TspRI	CASTG	5 cut(s)	393, 606, 901, 1234, 1384
VpaK11BI	GGWCC	2 cut(s)	1666, 1914
VspI	ATTAAT	1 cut(s)	1053
XapI	RAATTY	4 cut(s)	655, 803, 2296, 2547
XbaI	TCTAGA	2 cut(s)	613, 842
XceI	RCATGY	1 cut(s)	767
XcmI	CCANNNNNNNNNTGG	1 cut(s)	262
XmiI	GTMKAC	2 cut(s)	1235, 2102
Zsp2I	ATGCAT	1 cut(s)	1846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.