Rh4AG146700

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014761
Physical Location & Seq
Forward (+)
37064907 .. 37068028
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG146700.1

Sequence Viewer

Length: 1503 bp
ATGAAGAAAGCTCCTTTGTTAAAGAAACTGCTTAACAAAAGTGGCTCGTTATGGAAACTGTTTAACAAAAGTGGGTCTAATCCGGCTGAGAATGAAACTGACCAGTTGGGCTCAAATGAAGGGAGAGCATTTAACAATGAGGTTCGAATCGGAATCAATGAACCAGAGGTGATGCCTCACAGGAATGCTGTTCGAGAAGATGGGGGTTGTATGGATCAGAAGGTTAATAATGGTGCACCTGTTCAGGATATGGTTATTTTTAGCGGCGGTGTTCATCATCAGAGGCAGCAGCAAATTGAAAATAGTGGGGCAGATCATGTTGTTATCCATATTGAAGAAGGTGTGGTAGTTTGGGTTATAGTACCTAACATAATCCCGAGGCTGAATCTTGGAGCTGCGAACTTGGAACGCGCTTATTCATTGCTTTCTGTAGGAGGGATCATAACTCCATTTCACAATTTTATGAACTTCTGGCAGGGCAATGGTCAAGGATTGAGGCAGCAGCTCTACAATCTTATTCTGAGGCAGCAGCAGAATTATAATATTGGTGCACAGGGTGATAATGATTTTCACCAGCAACTAGTTCTGGACATTAAAGAAGCAGAAGGAGGAGTGGTAGGTGGGGGTAGGAGGATACCAAGGATCAGAGTGGCAGGTGGTGCTAGGAGGATACCAAGAATGATCCATGTGAGGTTGAGCGGTGGAGATGTGCCTGTCCATTCCACTTACGCATGGCTTTGTGAGAAAGGGCAATCAATTCCATTCAATGCTAGTGTGCATTCAGCGTATGGGTATGGTCAACAATCAATCTGGTTTGAGGAGGGAGGGCTTCCTCCGGCTGTTAAACCTTTTGCACCAGATACTGCAGACTTCCTCACCAAATCAAGACCTTATTTCTGTCAGGGATTGATATCGCTGGTCGTATTTTTCCTTGTTAGCTCACTACCGTACTTTTTCTTGAAGGGCTCAGGAGAAATAATTGGACCTAAAAATGATTCCAACGCCGACCCATTTTATTTCAGCATGGTCATAATGTCTTCCACAGGATACGGCGATATGTATCCCAAATCTACCCGTGCCAGACTGTTGACTTGCTTTTGGATACTTTTTGGTGTTGGAATTGTCGCTGGGCGATTAAGTGCCGTGTTTAACTGCTTAGCCAATGCCTTTGGCAACCTGTTTTTTGGATACATGAGTCGTCAATCTTCAGGTCAACTAATAACTGAAAGGTCCAAGGCAAAATACGAAGTTTGGTTCGCTGTGTTAGTGGTCGCCCTATGCATCGTTGTAGGTCTCTTTGGCTGCAAGTATTTGGAAGGAATGGACTGGCTGGAGTCCTTTTACTTGTCAGTGGTTACAGTATCATCAGTTGGTTTTGGAGATCTGCACTTTAATTCTTTAGCTGGAAAGGTTTTTGCGACTTTCTGGATACCAGTAAGCACCATTCTAGTTGGAATTTCATTTGGTTATATTTCAAAATGTGGATATGGAAGCTTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006873 GO:0006875 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009705 GO:0009987 GO:0010029 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019725 GO:0022607 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022840 GO:0022841 GO:0022842 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030007 GO:0030322 GO:0031004 GO:0031090 GO:0031224 GO:0031226 GO:0032991 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042592 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0048580 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051239 GO:0051259 GO:0051260 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0090533 GO:0098533 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099094 GO:1900140 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1902495 GO:1904949 GO:1990351 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

500

Amino Acids

55.11

Weight (kDa)

8.88

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 307 - 386 6.6e-13 Ion channel
Ion_trans_2 PF07885 420 - 493 1e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AarI CACCTGC 1 cut(s) 644
Acc36I ACCTGC 1 cut(s) 644
AccBSI CCGCTC 1 cut(s) 699
AccII CGCG 1 cut(s) 411
AciI CCGC 3 cut(s) 264, 267, 699
AclWI GGATC 4 cut(s) 222, 446, 650, 676
AcsI RAATTY 1 cut(s) 1455
AcuI CTGAAG 1 cut(s) 1191
AdeI CACNNNGTG 1 cut(s) 557
AfaI GTAC 2 cut(s) 363, 950
AflII CTTAAG 1 cut(s) 1495
AgsI TTSAA 5 cut(s) 299, 335, 766, 961, 1476
AhlI ACTAGT 1 cut(s) 580
AluBI AGCT 6 cut(s) 11, 395, 505, 939, 1403, 1494
AluI AGCT 6 cut(s) 11, 395, 505, 939, 1403, 1494
Alw21I GWGCWC 2 cut(s) 238, 553
Alw26I GTCTC 1 cut(s) 1298
Alw44I GTGCAC 2 cut(s) 234, 549
AlwI GGATC 4 cut(s) 222, 446, 650, 676
AlwNI CAGNNNCTG 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 376
ApaLI GTGCAC 2 cut(s) 234, 549
ApeKI GCWGC 8 cut(s) 286, 289, 395, 499, 502, 526, 529, 1302
ApoI RAATTY 1 cut(s) 1455
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 2 cut(s) 983, 1230
AsuHPI GGTGA 4 cut(s) 181, 563, 569, 868
AsuII TTCGAA 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 2 cut(s) 983, 1230
BaeGI GKGCMC 2 cut(s) 238, 553
BaeI ACNNNNGTAYC 2 cut(s) 1180, 1213
BanII GRGCYC 2 cut(s) 113, 968
BbsI GAAGAC 1 cut(s) 1029
Bbv12I GWGCWC 2 cut(s) 238, 553
BbvI GCAGC 8 cut(s) 298, 301, 382, 511, 514, 538, 541, 1289
BccI CCATC 1 cut(s) 194
BceAI ACGGC 2 cut(s) 1066, 1127
BciVI GTATCC 7 cut(s) 627, 663, 1040, 1071, 1095, 1181, 1422
BcoDI GTCTC 1 cut(s) 1298
BcuI ACTAGT 1 cut(s) 580
BfaI CTAG 4 cut(s) 581, 663, 771, 1448
BfmI CTRYAG 2 cut(s) 429, 864
BfrI CTTAAG 1 cut(s) 1495
BfuAI ACCTGC 1 cut(s) 644
BfuI GTATCC 7 cut(s) 627, 663, 1040, 1071, 1095, 1181, 1422
BglII AGATCT 1 cut(s) 1381
BisI GCNGC 9 cut(s) 265, 287, 290, 396, 500, 503, 527, 530, 1303
BlpI GCTNAGC 1 cut(s) 1156
BlsI GCNGC 9 cut(s) 266, 288, 291, 397, 501, 504, 528, 531, 1304
Bme18I GGWCC 2 cut(s) 983, 1230
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 983, 1230
BmsI GCATC 2 cut(s) 162, 1290
BpiI GAAGAC 1 cut(s) 1029
BpmI CTGGAG 1 cut(s) 1352
Bpu10I CCTNAGC 1 cut(s) 967
Bpu1102I GCTNAGC 1 cut(s) 1156
Bpu14I TTCGAA 1 cut(s) 145
BsaBI GATNNNNATC 2 cut(s) 910, 1059
BsaI GGTCTC 1 cut(s) 1298
BsaJI CCNNGG 3 cut(s) 377, 638, 1233
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bse1I ACTGG 3 cut(s) 103, 1331, 1433
Bse3DI GCAATG 2 cut(s) 419, 487
Bse8I GATNNNNATC 2 cut(s) 910, 1059
BseDI CCNNGG 3 cut(s) 377, 638, 1233
BseJI GATNNNNATC 2 cut(s) 910, 1059
BseMI GCAATG 2 cut(s) 419, 487
BseMII CTCAG 3 cut(s) 78, 512, 981
BseNI ACTGG 3 cut(s) 103, 1331, 1433
BseRI GAGGAG 2 cut(s) 624, 833
BseSI GKGCMC 2 cut(s) 238, 553
BseXI GCAGC 8 cut(s) 298, 301, 382, 511, 514, 538, 541, 1289
BseYI CCCAGC 1 cut(s) 1127
BsgI GTGCAG 1 cut(s) 1370
Bsh1236I CGCG 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 238, 553
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 2 cut(s) 83, 836
BsmAI GTCTC 1 cut(s) 1298
BsmI GAATGC 2 cut(s) 190, 778
Bso31I GGTCTC 1 cut(s) 1298
BsoBI CYCGRG 1 cut(s) 376
Bsp119I TTCGAA 1 cut(s) 145
Bsp1286I GDGCHC 4 cut(s) 113, 238, 553, 968
Bsp143I GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
Bsp1720I GCTNAGC 1 cut(s) 1156
BspACI CCGC 3 cut(s) 264, 267, 699
BspCNI CTCAG 3 cut(s) 79, 513, 980
BspFNI CGCG 1 cut(s) 411
BspMAI CTGCAG 1 cut(s) 868
BspMI ACCTGC 1 cut(s) 644
BspPI GGATC 4 cut(s) 222, 446, 650, 676
BspT104I TTCGAA 1 cut(s) 145
BspTI CTTAAG 1 cut(s) 1495
BspTNI GGTCTC 1 cut(s) 1298
BsrBI CCGCTC 1 cut(s) 699
BsrDI GCAATG 2 cut(s) 419, 487
BsrI ACTGG 3 cut(s) 103, 1331, 1433
BssECI CCNNGG 3 cut(s) 377, 638, 1233
BssMI GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
BssT1I CCWWGG 2 cut(s) 638, 1233
Bst4CI ACNGT 4 cut(s) 60, 948, 1086, 1360
BstAFI CTTAAG 1 cut(s) 1495
BstAPI GCANNNNNTGC 1 cut(s) 659
BstBI TTCGAA 1 cut(s) 145
BstDEI CTNAG 4 cut(s) 87, 521, 967, 1156
BstFNI CGCG 1 cut(s) 411
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 6 cut(s) 217, 316, 441, 645, 684, 1384
BstMAI GTCTC 1 cut(s) 1298
BstMBI GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstSFI CTRYAG 2 cut(s) 429, 864
BstSLI GKGCMC 2 cut(s) 238, 553
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 8 cut(s) 298, 301, 382, 511, 514, 538, 541, 1289
BstV2I GAAGAC 1 cut(s) 1029
BstX2I RGATCY 1 cut(s) 1381
BstYI RGATCY 1 cut(s) 1381
BsuI GTATCC 7 cut(s) 627, 663, 1040, 1071, 1095, 1181, 1422
BtsIMutI CAGTG 1 cut(s) 1356
BveI ACCTGC 1 cut(s) 644
CaiI CAGNNNCTG 1 cut(s) 863
CfoI GCGC 1 cut(s) 413
Cfr13I GGNCC 2 cut(s) 983, 1230
Csp6I GTAC 2 cut(s) 362, 949
CviAII CATG 5 cut(s) 317, 686, 732, 1024, 1192
CviQI GTAC 2 cut(s) 362, 949
DdeI CTNAG 4 cut(s) 87, 521, 967, 1156
DpnI GATC 6 cut(s) 216, 315, 440, 644, 683, 1383
DpnII GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
DraIII CACNNNGTG 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 638, 1233
Eco24I GRGCYC 2 cut(s) 113, 968
Eco31I GGTCTC 1 cut(s) 1298
Eco32I GATATC 1 cut(s) 912
Eco47I GGWCC 2 cut(s) 983, 1230
Eco57I CTGAAG 1 cut(s) 1191
Eco88I CYCGRG 1 cut(s) 376
EcoRV GATATC 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 638, 1233
EcoT22I ATGCAT 1 cut(s) 1283
EcoT38I GRGCYC 2 cut(s) 113, 968
ErhI CCWWGG 2 cut(s) 638, 1233
FaeI CATG 5 cut(s) 320, 689, 735, 1027, 1195
FatI CATG 5 cut(s) 316, 685, 731, 1023, 1191
Fnu4HI GCNGC 9 cut(s) 265, 287, 290, 396, 500, 503, 527, 530, 1303
FriOI GRGCYC 2 cut(s) 113, 968
Fsp4HI GCNGC 9 cut(s) 265, 287, 290, 396, 500, 503, 527, 530, 1303
FspBI CTAG 4 cut(s) 581, 663, 771, 1448
GlaI GCGC 1 cut(s) 412
GluI GCNGC 9 cut(s) 265, 287, 290, 396, 500, 503, 527, 530, 1303
GsaI CCCAGC 1 cut(s) 1131
GsuI CTGGAG 1 cut(s) 1352
HapII CCGG 2 cut(s) 83, 836
HhaI GCGC 1 cut(s) 413
Hin1II CATG 5 cut(s) 320, 689, 735, 1027, 1195
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HincII GTYRAC 3 cut(s) 800, 1089, 1214
HindII GTYRAC 3 cut(s) 800, 1089, 1214
HindIII AAGCTT 1 cut(s) 1492
HinfI GANTC 6 cut(s) 147, 153, 385, 995, 1195, 1334
HpaII CCGG 2 cut(s) 83, 836
HphI GGTGA 4 cut(s) 181, 563, 569, 868
Hpy166II GTNNAC 5 cut(s) 236, 551, 800, 1089, 1214
Hpy188I TCNGA 5 cut(s) 152, 219, 282, 522, 647
Hpy188III TCNNGA 8 cut(s) 194, 245, 376, 587, 885, 958, 969, 1426
Hpy8I GTNNAC 5 cut(s) 236, 551, 800, 1089, 1214
HpyAV CCTTC 6 cut(s) 113, 214, 332, 599, 955, 1310
HpyCH4III ACNGT 4 cut(s) 60, 948, 1086, 1360
HpyCH4V TGCA 8 cut(s) 236, 551, 778, 854, 866, 1281, 1305, 1387
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 4 cut(s) 87, 521, 967, 1156
Hsp92II CATG 5 cut(s) 320, 689, 735, 1027, 1195
HspAI GCGC 1 cut(s) 411
Kzo9I GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
LmnI GCTCC 2 cut(s) 16, 392
Lsp1109I GCAGC 8 cut(s) 298, 301, 382, 511, 514, 538, 541, 1289
LweI GCATC 2 cut(s) 162, 1290
MaeI CTAG 4 cut(s) 581, 663, 771, 1448
MaeIII GTNAC 1 cut(s) 1354
MalI GATC 6 cut(s) 216, 315, 440, 644, 683, 1383
MbiI CCGCTC 1 cut(s) 699
MboI GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
MboII GAAGA 5 cut(s) 16, 209, 347, 1029, 1197
MflI RGATCY 1 cut(s) 1381
MhlI GDGCHC 4 cut(s) 113, 238, 553, 968
MluCI AATT 8 cut(s) 294, 457, 535, 756, 978, 1119, 1393, 1455
MlyI GAGTC 2 cut(s) 1204, 1343
MmeI TCCRAC 3 cut(s) 1023, 1096, 1432
Mph1103I ATGCAT 1 cut(s) 1283
MslI CAYNNNNRTG 1 cut(s) 183
MspCI CTTAAG 1 cut(s) 1495
MspI CCGG 2 cut(s) 83, 836
Mva1269I GAATGC 2 cut(s) 190, 778
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 659
NdeII GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
NlaIII CATG 5 cut(s) 320, 689, 735, 1027, 1195
NsiI ATGCAT 1 cut(s) 1283
NspV TTCGAA 1 cut(s) 145
PaqCI CACCTGC 1 cut(s) 644
PctI GAATGC 2 cut(s) 190, 778
PfeI GAWTC 4 cut(s) 147, 153, 385, 995
PkrI GCNGC 9 cut(s) 266, 288, 291, 397, 501, 504, 528, 531, 1304
PleI GAGTC 2 cut(s) 1203, 1342
PpsI GAGTC 2 cut(s) 1203, 1342
PsiI TTATAA 1 cut(s) 540
PspFI CCCAGC 1 cut(s) 1127
PspPI GGNCC 2 cut(s) 983, 1230
PstI CTGCAG 1 cut(s) 868
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 1 cut(s) 1381
RsaI GTAC 2 cut(s) 363, 950
RsaNI GTAC 2 cut(s) 362, 949
RseI CAYNNNNRTG 1 cut(s) 183
SatI GCNGC 9 cut(s) 265, 287, 290, 396, 500, 503, 527, 530, 1303
Sau3AI GATC 6 cut(s) 214, 313, 438, 642, 681, 1381
Sau96I GGNCC 2 cut(s) 983, 1230
SchI GAGTC 2 cut(s) 1204, 1343
SduI GDGCHC 4 cut(s) 113, 238, 553, 968
SfaNI GCATC 2 cut(s) 162, 1290
SfcI CTRYAG 2 cut(s) 429, 864
SfuI TTCGAA 1 cut(s) 145
SinI GGWCC 2 cut(s) 983, 1230
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 1495
SmoI CTYRAG 1 cut(s) 1495
SpeI ACTAGT 1 cut(s) 580
Sse9I AATT 8 cut(s) 294, 457, 535, 756, 978, 1119, 1393, 1455
SsiI CCGC 3 cut(s) 264, 267, 699
SspI AATATT 1 cut(s) 544
SspMI CTAG 4 cut(s) 581, 663, 771, 1448
StyI CCWWGG 2 cut(s) 638, 1233
TaaI ACNGT 4 cut(s) 60, 948, 1086, 1360
TaqI TCGA 2 cut(s) 145, 193
TasI AATT 8 cut(s) 294, 457, 535, 756, 978, 1119, 1393, 1455
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 4 cut(s) 147, 153, 385, 995
TscAI CASTG 1 cut(s) 1356
TseI GCWGC 8 cut(s) 286, 289, 395, 499, 502, 526, 529, 1302
TspDTI ATGAA 8 cut(s) 17, 108, 132, 174, 263, 408, 479, 1449
TspRI CASTG 1 cut(s) 1356
Vha464I CTTAAG 1 cut(s) 1495
VneI GTGCAC 2 cut(s) 234, 549
VpaK11BI GGWCC 2 cut(s) 983, 1230
XapI RAATTY 1 cut(s) 1455
XspI CTAG 4 cut(s) 581, 663, 771, 1448
Zsp2I ATGCAT 1 cut(s) 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.