Rh4DG140700

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014761
Physical Location & Seq
Forward (+)
27914825 .. 27917943
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG140700.1

Sequence Viewer

Length: 1560 bp
ATGACAGGTGATACAAATTCCTGGGGCAGAAAAAAACAACAAGTTTCAGGTGCTCCTGGGGTGAAGAAGGTTCCTTGGTTAAAGAAACTGCTTAACAAAAGTGGCTCGTTAAGGAAACTGTTTAACAAAAGTGGGTCTAATCCGGCTGAGAATGAAACTGACCAGTTGGGCTCAAATGAAGGGAGAACATTTAACAATGAGGTTCGAATCGGAATCAAGGAACCAGAGGTGCCTCACAGGAATGCTGTTCAAGAAGATGGGGGTTGTATTGATCAGAAGGTTAATAATGGTGCACCTGTTCAGGATATGGTTATTTTTAGTGGCGGTGTTCATCATCAGAGGCAGCAGCAAATTGAAAATAGTGGGGCAGATCATGTTGTTATCCATATTGAAGAAGGTGTGGTAGGTTGGGTTATAGTACCTAACATAATCCCAAGGCCGAATCTTGGAGCTGGGAACTTGGAACGCGCTTATTCATTGCTTTCTGTAGGAGGGATCATAACTCCATTTCACAATTTTATGAACTTCTGGCAGGGCAATGGTCAAGGATTGAGGCAGCAGCTCTACAATCTTATTCTGAGGCAGCAGCAGAATTATAATATTGGTGCACAGGGTGATAATGATTTTCACCATCAACTAGTTCTGGACATTAAAGAAGCAGAAGGAGGAGTGGTAGGTGGGGGTAGGAGGATACCAAGGATCAGAGTGGCAGGTGGTGCTAGGAGGATACCAAGAATGATCCTTGTGAGGTTGAGCGGTGGAGATGTGCCTGTCCATTCCACTTACGCATGGCTTTGTGAGAAAGGGCAATCAATTCCATTCAATGCTAGTGTGCATTCAGCGTATGGGTATGGTCAACAATCAATCTGGTTTGAGGAGGGAGGGCTTCCTCCGGCTGTTGAACCTTTTGCACCAGATACTGCAGACTTCCTCACCAAATCAAGTCCTTATTTCTGGCAGGGATTTATATCGCTGGTCATATTTTTGCTTGTTAGCTCACTACCGTACGTTTTCTTGAAGGACTCAGGAGAAATAATTGGACCTCTAGATGATTGCGACGCCGACCCATTTTATTTCAGCATGGTCATAATGTCTTCCACAGGATACGGCGATATGTATCCCAAATCTACCCGTGCCAGACTGTTGACTTGCTTTTGGATACTTTTTGGTGTTGGAATTGTCGCTGGGCGATTAAGTGCCGTGTTTAACTGCTTAGCCAATGCCTTTGGCAACCTTTTTTTTGGATACATGAGTCGTCGATCTTCAGGTCAACTAATAACTGAAAGGTCCAGGGCAAAATACAAAGTTTGGTTTGCTGTGTTAGTGGTCGCCCTATGCATTGTTGTAGGTCTCTTTGGCTGCAAGTATTTGGAAGGAATGAACTGGCTGGAGTCCTTTTACTTGTCAGTGGTTACAGTATCATCAGTTGGTTTTGGAGATCTTCACTTTAATTCTCTTGCTGGAAAGGTTTTTGCGACTTTCTGGATACCAGTAAGCACCATTCTAGTTGGAATTTCATTTGGTTATATTTCAAAATGTGGATATGGAAGCTTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

519

Amino Acids

57.11

Weight (kDa)

8.87

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 355 - 405 7.3e-12 Ion channel
Ion_trans_2 PF07885 439 - 512 1.2e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
AarI CACCTGC 1 cut(s) 701
Acc36I ACCTGC 1 cut(s) 701
AccB1I GGYRCC 1 cut(s) 229
AccBSI CCGCTC 1 cut(s) 756
AccII CGCG 1 cut(s) 468
AciI CCGC 2 cut(s) 324, 756
AclWI GGATC 3 cut(s) 503, 707, 733
AcsI RAATTY 2 cut(s) 16, 1512
AcuI CTGAAG 1 cut(s) 1248
AcyI GRCGYC 1 cut(s) 1059
AdeI CACNNNGTG 1 cut(s) 614
AfaI GTAC 2 cut(s) 420, 1007
AfiI CCNNNNNNNGG 1 cut(s) 446
AflII CTTAAG 1 cut(s) 1552
AgsI TTSAA 7 cut(s) 251, 356, 392, 823, 902, 1018, 1533
AhlI ACTAGT 1 cut(s) 637
AjnI CCWGG 3 cut(s) 20, 55, 1289
AluBI AGCT 4 cut(s) 452, 562, 996, 1551
AluI AGCT 4 cut(s) 452, 562, 996, 1551
Alw21I GWGCWC 3 cut(s) 55, 295, 610
Alw26I GTCTC 1 cut(s) 1355
Alw44I GTGCAC 2 cut(s) 291, 606
AlwI GGATC 3 cut(s) 503, 707, 733
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 1 cut(s) 437
ApaLI GTGCAC 2 cut(s) 291, 606
ApeKI GCWGC 7 cut(s) 343, 346, 556, 559, 583, 586, 1359
ApoI RAATTY 2 cut(s) 16, 1512
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 2 cut(s) 1040, 1287
AsuHPI GGTGA 5 cut(s) 20, 73, 620, 626, 925
AsuII TTCGAA 1 cut(s) 205
AvaII GGWCC 2 cut(s) 1040, 1287
BaeGI GKGCMC 2 cut(s) 295, 610
BaeI ACNNNNGTAYC 2 cut(s) 1237, 1270
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 1086
Bbv12I GWGCWC 3 cut(s) 55, 295, 610
BbvI GCAGC 7 cut(s) 355, 358, 568, 571, 595, 598, 1346
BccI CCATC 2 cut(s) 251, 639
BceAI ACGGC 2 cut(s) 1123, 1184
BciT130I CCWGG 3 cut(s) 22, 57, 1291
BciVI GTATCC 7 cut(s) 684, 720, 1097, 1128, 1152, 1238, 1479
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 1355
BcuI ACTAGT 1 cut(s) 637
BfaI CTAG 5 cut(s) 638, 720, 828, 1046, 1505
BfmI CTRYAG 2 cut(s) 486, 921
BfrI CTTAAG 1 cut(s) 1552
BfuAI ACCTGC 1 cut(s) 701
BfuI GTATCC 7 cut(s) 684, 720, 1097, 1128, 1152, 1238, 1479
BglII AGATCT 1 cut(s) 1438
BisI GCNGC 7 cut(s) 344, 347, 557, 560, 584, 587, 1360
BlpI GCTNAGC 1 cut(s) 1213
BlsI GCNGC 7 cut(s) 345, 348, 558, 561, 585, 588, 1361
Bme1390I CCNGG 3 cut(s) 22, 57, 1291
Bme18I GGWCC 2 cut(s) 1040, 1287
BmgT120I GGNCC 2 cut(s) 1040, 1287
BmiI GGNNCC 3 cut(s) 72, 222, 231
BmrFI CCNGG 3 cut(s) 22, 57, 1291
BpiI GAAGAC 1 cut(s) 1086
BpmI CTGGAG 1 cut(s) 1409
Bpu1102I GCTNAGC 1 cut(s) 1213
Bpu14I TTCGAA 1 cut(s) 205
BsaBI GATNNNNATC 2 cut(s) 967, 1116
BsaHI GRCGYC 1 cut(s) 1059
BsaI GGTCTC 1 cut(s) 1355
BsaJI CCNNGG 6 cut(s) 21, 56, 74, 434, 695, 1290
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse1I ACTGG 3 cut(s) 163, 1388, 1490
Bse3DI GCAATG 2 cut(s) 476, 544
Bse8I GATNNNNATC 2 cut(s) 967, 1116
BseBI CCWGG 3 cut(s) 22, 57, 1291
BseDI CCNNGG 6 cut(s) 21, 56, 74, 434, 695, 1290
BseJI GATNNNNATC 2 cut(s) 967, 1116
BseLI CCNNNNNNNGG 1 cut(s) 446
BseMI GCAATG 2 cut(s) 476, 544
BseMII CTCAG 3 cut(s) 138, 569, 1038
BseNI ACTGG 3 cut(s) 163, 1388, 1490
BseRI GAGGAG 2 cut(s) 681, 890
BseSI GKGCMC 2 cut(s) 295, 610
BseXI GCAGC 7 cut(s) 355, 358, 568, 571, 595, 598, 1346
BseYI CCCAGC 2 cut(s) 452, 1184
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 229
BsiHKAI GWGCWC 3 cut(s) 55, 295, 610
BsiSI CCGG 2 cut(s) 143, 893
BsiWI CGTACG 1 cut(s) 1005
BslI CCNNNNNNNGG 1 cut(s) 446
BsmAI GTCTC 1 cut(s) 1355
BsmI GAATGC 2 cut(s) 247, 835
BsnI GGCC 1 cut(s) 439
Bso31I GGTCTC 1 cut(s) 1355
Bsp119I TTCGAA 1 cut(s) 205
Bsp1286I GDGCHC 4 cut(s) 55, 173, 295, 610
Bsp143I GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
Bsp1720I GCTNAGC 1 cut(s) 1213
BspACI CCGC 2 cut(s) 324, 756
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 3 cut(s) 139, 570, 1037
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 3 cut(s) 72, 222, 231
BspMAI CTGCAG 1 cut(s) 925
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 3 cut(s) 503, 707, 733
BspT104I TTCGAA 1 cut(s) 205
BspT107I GGYRCC 1 cut(s) 229
BspTI CTTAAG 1 cut(s) 1552
BspTNI GGTCTC 1 cut(s) 1355
BsrBI CCGCTC 1 cut(s) 756
BsrDI GCAATG 2 cut(s) 476, 544
BsrI ACTGG 3 cut(s) 163, 1388, 1490
BssECI CCNNGG 6 cut(s) 21, 56, 74, 434, 695, 1290
BssMI GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
BssNI GRCGYC 1 cut(s) 1059
BssT1I CCWWGG 3 cut(s) 74, 434, 695
Bst2UI CCWGG 3 cut(s) 22, 57, 1291
Bst4CI ACNGT 4 cut(s) 120, 1005, 1143, 1417
BstACI GRCGYC 1 cut(s) 1059
BstAFI CTTAAG 1 cut(s) 1552
BstAPI GCANNNNNTGC 1 cut(s) 716
BstBI TTCGAA 1 cut(s) 205
BstDEI CTNAG 4 cut(s) 147, 578, 1024, 1213
BstFNI CGCG 1 cut(s) 468
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 7 cut(s) 274, 373, 498, 702, 741, 1262, 1441
BstMAI GTCTC 1 cut(s) 1355
BstMBI GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
BstMWI GCNNNNNNNGC 1 cut(s) 716
BstNI CCWGG 3 cut(s) 22, 57, 1291
BstSCI CCNGG 3 cut(s) 20, 55, 1289
BstSFI CTRYAG 2 cut(s) 486, 921
BstSLI GKGCMC 2 cut(s) 295, 610
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 7 cut(s) 355, 358, 568, 571, 595, 598, 1346
BstV2I GAAGAC 1 cut(s) 1086
BstX2I RGATCY 1 cut(s) 1438
BstYI RGATCY 1 cut(s) 1438
BsuI GTATCC 7 cut(s) 684, 720, 1097, 1128, 1152, 1238, 1479
BsuRI GGCC 1 cut(s) 439
BtsIMutI CAGTG 1 cut(s) 1413
BveI ACCTGC 1 cut(s) 701
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 1040, 1287
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 2 cut(s) 419, 1006
CviAII CATG 4 cut(s) 374, 789, 1081, 1249
CviQI GTAC 2 cut(s) 419, 1006
DdeI CTNAG 4 cut(s) 147, 578, 1024, 1213
DpnI GATC 7 cut(s) 273, 372, 497, 701, 740, 1261, 1440
DpnII GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
DraIII CACNNNGTG 1 cut(s) 614
Eco130I CCWWGG 3 cut(s) 74, 434, 695
Eco24I GRGCYC 1 cut(s) 173
Eco31I GGTCTC 1 cut(s) 1355
Eco47I GGWCC 2 cut(s) 1040, 1287
Eco57I CTGAAG 1 cut(s) 1248
EcoRII CCWGG 3 cut(s) 20, 55, 1289
EcoT14I CCWWGG 3 cut(s) 74, 434, 695
EcoT22I ATGCAT 1 cut(s) 1340
EcoT38I GRGCYC 1 cut(s) 173
ErhI CCWWGG 3 cut(s) 74, 434, 695
FaeI CATG 4 cut(s) 377, 792, 1084, 1252
FatI CATG 4 cut(s) 373, 788, 1080, 1248
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 7 cut(s) 344, 347, 557, 560, 584, 587, 1360
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 7 cut(s) 344, 347, 557, 560, 584, 587, 1360
FspBI CTAG 5 cut(s) 638, 720, 828, 1046, 1505
GlaI GCGC 1 cut(s) 469
GluI GCNGC 7 cut(s) 344, 347, 557, 560, 584, 587, 1360
GsaI CCCAGC 2 cut(s) 456, 1188
GsuI CTGGAG 1 cut(s) 1409
HaeIII GGCC 1 cut(s) 439
HapII CCGG 2 cut(s) 143, 893
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 1 cut(s) 470
Hin1I GRCGYC 1 cut(s) 1059
Hin1II CATG 4 cut(s) 377, 792, 1084, 1252
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 3 cut(s) 857, 1146, 1271
HindII GTYRAC 3 cut(s) 857, 1146, 1271
HindIII AAGCTT 1 cut(s) 1549
HinfI GANTC 6 cut(s) 207, 213, 442, 1022, 1252, 1391
HpaII CCGG 2 cut(s) 143, 893
HphI GGTGA 5 cut(s) 20, 73, 620, 626, 925
Hpy166II GTNNAC 5 cut(s) 293, 608, 857, 1146, 1271
Hpy188I TCNGA 5 cut(s) 212, 276, 339, 579, 704
Hpy188III TCNNGA 7 cut(s) 251, 302, 644, 1015, 1026, 1046, 1483
Hpy8I GTNNAC 5 cut(s) 293, 608, 857, 1146, 1271
Hpy99I CGWCG 2 cut(s) 1061, 1260
HpyAV CCTTC 7 cut(s) 61, 173, 271, 389, 656, 1012, 1367
HpyCH4III ACNGT 4 cut(s) 120, 1005, 1143, 1417
HpyCH4IV ACGT 1 cut(s) 1008
HpyCH4V TGCA 7 cut(s) 293, 608, 835, 911, 923, 1338, 1362
HpyF10VI GCNNNNNNNGC 1 cut(s) 716
HpyF3I CTNAG 4 cut(s) 147, 578, 1024, 1213
HpySE526I ACGT 1 cut(s) 1008
Hsp92I GRCGYC 1 cut(s) 1059
Hsp92II CATG 4 cut(s) 377, 792, 1084, 1252
HspAI GCGC 1 cut(s) 468
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
LmnI GCTCC 2 cut(s) 58, 449
Lsp1109I GCAGC 7 cut(s) 355, 358, 568, 571, 595, 598, 1346
MaeI CTAG 5 cut(s) 638, 720, 828, 1046, 1505
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 1 cut(s) 1411
MalI GATC 7 cut(s) 273, 372, 497, 701, 740, 1261, 1440
MbiI CCGCTC 1 cut(s) 756
MboI GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
MboII GAAGA 6 cut(s) 76, 266, 404, 1086, 1254, 1433
MflI RGATCY 1 cut(s) 1438
MhlI GDGCHC 4 cut(s) 55, 173, 295, 610
MluCI AATT 9 cut(s) 16, 351, 514, 592, 813, 1035, 1176, 1450, 1512
MlyI GAGTC 3 cut(s) 1016, 1261, 1400
MmeI TCCRAC 2 cut(s) 1153, 1489
Mph1103I ATGCAT 1 cut(s) 1340
MslI CAYNNNNRTG 1 cut(s) 240
MspCI CTTAAG 1 cut(s) 1552
MspI CCGG 2 cut(s) 143, 893
MspR9I CCNGG 3 cut(s) 22, 57, 1291
Mva1269I GAATGC 2 cut(s) 247, 835
MvaI CCWGG 3 cut(s) 22, 57, 1291
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 1 cut(s) 716
NdeII GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
NlaIII CATG 4 cut(s) 377, 792, 1084, 1252
NlaIV GGNNCC 3 cut(s) 72, 222, 231
NsiI ATGCAT 1 cut(s) 1340
NspV TTCGAA 1 cut(s) 205
PaqCI CACCTGC 1 cut(s) 701
PctI GAATGC 2 cut(s) 247, 835
PfeI GAWTC 3 cut(s) 207, 213, 442
Pfl23II CGTACG 1 cut(s) 1005
PkrI GCNGC 7 cut(s) 345, 348, 558, 561, 585, 588, 1361
PleI GAGTC 3 cut(s) 1016, 1260, 1399
PpsI GAGTC 3 cut(s) 1016, 1260, 1399
PsiI TTATAA 1 cut(s) 597
Psp6I CCWGG 3 cut(s) 20, 55, 1289
PspFI CCCAGC 2 cut(s) 452, 1184
PspGI CCWGG 3 cut(s) 20, 55, 1289
PspLI CGTACG 1 cut(s) 1005
PspN4I GGNNCC 3 cut(s) 72, 222, 231
PspPI GGNCC 2 cut(s) 1040, 1287
PstI CTGCAG 1 cut(s) 925
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 1438
RsaI GTAC 2 cut(s) 420, 1007
RsaNI GTAC 2 cut(s) 419, 1006
RseI CAYNNNNRTG 1 cut(s) 240
SatI GCNGC 7 cut(s) 344, 347, 557, 560, 584, 587, 1360
Sau3AI GATC 7 cut(s) 271, 370, 495, 699, 738, 1259, 1438
Sau96I GGNCC 2 cut(s) 1040, 1287
SchI GAGTC 3 cut(s) 1016, 1261, 1400
ScrFI CCNGG 3 cut(s) 22, 57, 1291
SduI GDGCHC 4 cut(s) 55, 173, 295, 610
SfcI CTRYAG 2 cut(s) 486, 921
SfuI TTCGAA 1 cut(s) 205
SinI GGWCC 2 cut(s) 1040, 1287
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 1552
SmoI CTYRAG 1 cut(s) 1552
SpeI ACTAGT 1 cut(s) 637
Sse9I AATT 9 cut(s) 16, 351, 514, 592, 813, 1035, 1176, 1450, 1512
SsiI CCGC 2 cut(s) 324, 756
SspI AATATT 1 cut(s) 601
SspMI CTAG 5 cut(s) 638, 720, 828, 1046, 1505
StyD4I CCNGG 3 cut(s) 20, 55, 1289
StyI CCWWGG 3 cut(s) 74, 434, 695
TaaI ACNGT 4 cut(s) 120, 1005, 1143, 1417
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 2 cut(s) 205, 1258
TasI AATT 9 cut(s) 16, 351, 514, 592, 813, 1035, 1176, 1450, 1512
TfiI GAWTC 3 cut(s) 207, 213, 442
TscAI CASTG 1 cut(s) 1413
TseI GCWGC 7 cut(s) 343, 346, 556, 559, 583, 586, 1359
TspDTI ATGAA 7 cut(s) 168, 192, 320, 465, 536, 1394, 1506
TspRI CASTG 1 cut(s) 1413
Vha464I CTTAAG 1 cut(s) 1552
VneI GTGCAC 2 cut(s) 291, 606
VpaK11BI GGWCC 2 cut(s) 1040, 1287
XapI RAATTY 2 cut(s) 16, 1512
XbaI TCTAGA 1 cut(s) 1045
XspI CTAG 5 cut(s) 638, 720, 828, 1046, 1505
Zsp2I ATGCAT 1 cut(s) 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.