Rw4G012040

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014761
Physical Location & Seq
Forward (+)
28349955 .. 28351427
1473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012040.1

Sequence Viewer

Length: 1473 bp
ATGAAGAAAGCTCCTTTGTTAAAGAAACTGCTTAACAAAAGTGGGTCTAATCCGGCTGAGAATGAAACTGACCAGTTGGGCTCAAATGAAGGGAGAGCATTTAACAATGAGGTTCGAATCAGAATCAATGAACCAGAGGTGATGCCTCACAGGAATGCTGTTCGAGAAGATGGGGATTGTATGGATCAGAAGGTTAATAATGGTGCACCTGTTCAGGATATGGTTATTTTTAGTGGCGGTGTTCATCATCAGAGGCAGCAGCAAATTGAAAATAGTTGGGCAGATCATGTTGTTATCCATATTGAAGAAGGTGTGGTAGGTCGGGTTATAGTACCTAACATAATCCCGAGGCTGAATCTTGGAGCTGGGAACTTGGAATGCGCTTATTCATTGCTTTCTGTAGGAGGGATCATAACTCCATTTCACAATTTTATGAACTTCTGGCAGGGCAATGGTCAAGGATTGAGGCAGCAGCTCTACAATCTTATTCTGAGGCAGCAGCAGAATTATAATATTGGTGCACAGGGTGATAATGATTTTCACCAGCAACTAGTTCTGGACATTAAAGAAGCAGAAGGAGGAGTGGTAGGTGGGGGTAGGAGGATACCAAGGATCAGAGTGGCAGGTGGTGCTAGGAGGATACCAAGAATGATCCATGTGAGGTTGAGCGGTGGAGATGTGCCTGTCCATTCCACTTACGCATGGCTTTGTGAGAAAGGGCAATCAATTCCATTCAATGCTAGTGTGCATTCAGCGTATGGGTATGGTCAACAATCAATCTGGTTTGAGGAGGGAGGGCTTCCTCCGGCTGTTGAACCTTTTGCACCAGATACTGCAGACTTCCTCACCAAATCACGTCCTTATTTCTGTCAGGGATTGATATCGCTGGTCGTATTTTTCCTTGTTAGCTCACTACCGTACTTTTTCTTGAAGGGCTCAGGAGAAATAATTGGACCTCTAGATGATTGCAATGCCGACCCATTTTATTTCAGCATGGTCATAATGTCTTCCACAGGATACGGCGATATGTATCCCAAATCTACCCGTGCCAGACTGTTGACTTGCTTTTGGATACTTTTTGGTGTTGGAATTGTCGCTGGGCGATTAAGTGCCGTGTTTAACTGCTTAGCCAATGCCTTTGGCAACCTTTTTTTCAGATACATGAGTCGTCGATCTTCAGGTCAACTAATAACTGAAAGGTGCAGGGCAAAATACAAAGTTTGGTTCGCTGTGTTAGTGGTCGCCCTATGCATCGTTGTAGGTCTCTTTGGCTGCAAGTATTTGGAAGGAATGGACTGGCTGGAGTCCTTTTACCTGTCAGTGGTTACAGTATCATCAGTTGGTTTTGGAGATCTGCACTTTAATTCTTTAGCTGGAAAGGTTTTTGCGACTGTCTGGATACCAGTAAGCACCATTCTAGTTGGAATTTCATTTGGTTATATTTCAAAATGTGGATATGGAAGCTTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

490

Amino Acids

54.22

Weight (kDa)

8.55

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 326 - 376 7.5e-12 Ion channel
Ion_trans_2 PF07885 410 - 483 1.1e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 510
AarI CACCTGC 1 cut(s) 614
Acc36I ACCTGC 1 cut(s) 614
AccBSI CCGCTC 1 cut(s) 669
AciI CCGC 2 cut(s) 237, 669
AclWI GGATC 4 cut(s) 192, 416, 620, 646
AcsI RAATTY 1 cut(s) 1425
AcuI CTGAAG 1 cut(s) 1161
AdeI CACNNNGTG 1 cut(s) 527
AfaI GTAC 2 cut(s) 333, 920
AfiI CCNNNNNNNGG 1 cut(s) 1321
AflII CTTAAG 1 cut(s) 1465
AgsI TTSAA 6 cut(s) 269, 305, 736, 815, 931, 1446
AhlI ACTAGT 1 cut(s) 550
AjiI CACGTC 1 cut(s) 857
AluBI AGCT 6 cut(s) 11, 365, 475, 909, 1373, 1464
AluI AGCT 6 cut(s) 11, 365, 475, 909, 1373, 1464
Alw21I GWGCWC 2 cut(s) 208, 523
Alw26I GTCTC 1 cut(s) 1268
Alw44I GTGCAC 2 cut(s) 204, 519
AlwI GGATC 4 cut(s) 192, 416, 620, 646
AlwNI CAGNNNCTG 1 cut(s) 833
Ama87I CYCGRG 1 cut(s) 346
ApaLI GTGCAC 2 cut(s) 204, 519
ApeKI GCWGC 7 cut(s) 256, 259, 469, 472, 496, 499, 1272
ApoI RAATTY 1 cut(s) 1425
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 953
AsuHPI GGTGA 4 cut(s) 151, 533, 539, 838
AsuII TTCGAA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 346
AvaII GGWCC 1 cut(s) 953
BaeGI GKGCMC 2 cut(s) 208, 523
BaeI ACNNNNGTAYC 2 cut(s) 1150, 1183
BanII GRGCYC 2 cut(s) 83, 938
BbsI GAAGAC 1 cut(s) 999
Bbv12I GWGCWC 2 cut(s) 208, 523
BbvI GCAGC 7 cut(s) 268, 271, 481, 484, 508, 511, 1259
BccI CCATC 1 cut(s) 164
BceAI ACGGC 2 cut(s) 1036, 1097
BciVI GTATCC 6 cut(s) 597, 633, 1010, 1041, 1065, 1392
BcoDI GTCTC 1 cut(s) 1268
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 5 cut(s) 551, 633, 741, 959, 1418
BfmI CTRYAG 2 cut(s) 399, 834
BfrI CTTAAG 1 cut(s) 1465
BfuAI ACCTGC 1 cut(s) 614
BfuI GTATCC 6 cut(s) 597, 633, 1010, 1041, 1065, 1392
BglII AGATCT 1 cut(s) 1351
BisI GCNGC 7 cut(s) 257, 260, 470, 473, 497, 500, 1273
BlpI GCTNAGC 1 cut(s) 1126
BlsI GCNGC 7 cut(s) 258, 261, 471, 474, 498, 501, 1274
Bme18I GGWCC 1 cut(s) 953
BmeT110I CYCGRG 1 cut(s) 346
BmgBI CACGTC 1 cut(s) 857
BmgT120I GGNCC 1 cut(s) 953
BmsI GCATC 2 cut(s) 132, 1260
BpiI GAAGAC 1 cut(s) 999
BpmI CTGGAG 1 cut(s) 1322
Bpu10I CCTNAGC 1 cut(s) 937
Bpu1102I GCTNAGC 1 cut(s) 1126
Bpu14I TTCGAA 1 cut(s) 115
BsaBI GATNNNNATC 2 cut(s) 880, 1029
BsaI GGTCTC 1 cut(s) 1268
BsaJI CCNNGG 2 cut(s) 347, 608
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 1 cut(s) 1321
Bse1I ACTGG 3 cut(s) 73, 1301, 1403
Bse3DI GCAATG 3 cut(s) 389, 457, 976
Bse8I GATNNNNATC 2 cut(s) 880, 1029
BseDI CCNNGG 2 cut(s) 347, 608
BseJI GATNNNNATC 2 cut(s) 880, 1029
BseLI CCNNNNNNNGG 1 cut(s) 1321
BseMI GCAATG 3 cut(s) 389, 457, 976
BseMII CTCAG 3 cut(s) 48, 482, 951
BseNI ACTGG 3 cut(s) 73, 1301, 1403
BseRI GAGGAG 2 cut(s) 594, 803
BseSI GKGCMC 2 cut(s) 208, 523
BseXI GCAGC 7 cut(s) 268, 271, 481, 484, 508, 511, 1259
BseYI CCCAGC 2 cut(s) 365, 1097
BsgI GTGCAG 2 cut(s) 1222, 1340
BsiHKAI GWGCWC 2 cut(s) 208, 523
BsiHKCI CYCGRG 1 cut(s) 346
BsiSI CCGG 2 cut(s) 53, 806
BslI CCNNNNNNNGG 1 cut(s) 1321
BsmAI GTCTC 1 cut(s) 1268
BsmI GAATGC 3 cut(s) 160, 383, 748
Bso31I GGTCTC 1 cut(s) 1268
BsoBI CYCGRG 1 cut(s) 346
Bsp119I TTCGAA 1 cut(s) 115
Bsp1286I GDGCHC 4 cut(s) 83, 208, 523, 938
Bsp143I GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
Bsp1720I GCTNAGC 1 cut(s) 1126
BspACI CCGC 2 cut(s) 237, 669
BspCNI CTCAG 3 cut(s) 49, 483, 950
BspMAI CTGCAG 1 cut(s) 838
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 4 cut(s) 192, 416, 620, 646
BspT104I TTCGAA 1 cut(s) 115
BspTI CTTAAG 1 cut(s) 1465
BspTNI GGTCTC 1 cut(s) 1268
BsrBI CCGCTC 1 cut(s) 669
BsrDI GCAATG 3 cut(s) 389, 457, 976
BsrI ACTGG 3 cut(s) 73, 1301, 1403
BssECI CCNNGG 2 cut(s) 347, 608
BssMI GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
BssT1I CCWWGG 1 cut(s) 608
Bst4CI ACNGT 4 cut(s) 918, 1056, 1330, 1393
BstAFI CTTAAG 1 cut(s) 1465
BstAPI GCANNNNNTGC 1 cut(s) 629
BstBI TTCGAA 1 cut(s) 115
BstDEI CTNAG 4 cut(s) 57, 491, 937, 1126
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 7 cut(s) 187, 286, 411, 615, 654, 1175, 1354
BstMAI GTCTC 1 cut(s) 1268
BstMBI GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
BstMWI GCNNNNNNNGC 1 cut(s) 629
BstSFI CTRYAG 2 cut(s) 399, 834
BstSLI GKGCMC 2 cut(s) 208, 523
BstV1I GCAGC 7 cut(s) 268, 271, 481, 484, 508, 511, 1259
BstV2I GAAGAC 1 cut(s) 999
BstX2I RGATCY 1 cut(s) 1351
BstYI RGATCY 1 cut(s) 1351
BsuI GTATCC 6 cut(s) 597, 633, 1010, 1041, 1065, 1392
BtrI CACGTC 1 cut(s) 857
BtsIMutI CAGTG 1 cut(s) 1326
BveI ACCTGC 1 cut(s) 614
CaiI CAGNNNCTG 1 cut(s) 833
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 953
Csp6I GTAC 2 cut(s) 332, 919
CviAII CATG 5 cut(s) 287, 656, 702, 994, 1162
CviQI GTAC 2 cut(s) 332, 919
DdeI CTNAG 4 cut(s) 57, 491, 937, 1126
DpnI GATC 7 cut(s) 186, 285, 410, 614, 653, 1174, 1353
DpnII GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
DraIII CACNNNGTG 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 608
Eco24I GRGCYC 2 cut(s) 83, 938
Eco31I GGTCTC 1 cut(s) 1268
Eco32I GATATC 1 cut(s) 882
Eco47I GGWCC 1 cut(s) 953
Eco57I CTGAAG 1 cut(s) 1161
Eco88I CYCGRG 1 cut(s) 346
EcoRV GATATC 1 cut(s) 882
EcoT14I CCWWGG 1 cut(s) 608
EcoT22I ATGCAT 1 cut(s) 1253
EcoT38I GRGCYC 2 cut(s) 83, 938
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 5 cut(s) 290, 659, 705, 997, 1165
FatI CATG 5 cut(s) 286, 655, 701, 993, 1161
Fnu4HI GCNGC 7 cut(s) 257, 260, 470, 473, 497, 500, 1273
FriOI GRGCYC 2 cut(s) 83, 938
Fsp4HI GCNGC 7 cut(s) 257, 260, 470, 473, 497, 500, 1273
FspBI CTAG 5 cut(s) 551, 633, 741, 959, 1418
GlaI GCGC 1 cut(s) 382
GluI GCNGC 7 cut(s) 257, 260, 470, 473, 497, 500, 1273
GsaI CCCAGC 2 cut(s) 369, 1101
GsuI CTGGAG 1 cut(s) 1322
HapII CCGG 2 cut(s) 53, 806
HhaI GCGC 1 cut(s) 383
Hin1II CATG 5 cut(s) 290, 659, 705, 997, 1165
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HincII GTYRAC 3 cut(s) 770, 1059, 1184
HindII GTYRAC 3 cut(s) 770, 1059, 1184
HindIII AAGCTT 1 cut(s) 1462
HinfI GANTC 5 cut(s) 117, 123, 355, 1165, 1304
HpaII CCGG 2 cut(s) 53, 806
HphI GGTGA 4 cut(s) 151, 533, 539, 838
Hpy166II GTNNAC 5 cut(s) 206, 521, 770, 1059, 1184
Hpy188I TCNGA 6 cut(s) 122, 189, 252, 492, 617, 1157
Hpy188III TCNNGA 8 cut(s) 164, 215, 346, 557, 928, 939, 959, 1396
Hpy8I GTNNAC 5 cut(s) 206, 521, 770, 1059, 1184
Hpy99I CGWCG 1 cut(s) 1173
HpyAV CCTTC 6 cut(s) 83, 184, 302, 569, 925, 1280
HpyCH4III ACNGT 4 cut(s) 918, 1056, 1330, 1393
HpyCH4IV ACGT 1 cut(s) 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 629
HpyF3I CTNAG 4 cut(s) 57, 491, 937, 1126
HpySE526I ACGT 1 cut(s) 856
Hsp92II CATG 5 cut(s) 290, 659, 705, 997, 1165
HspAI GCGC 1 cut(s) 381
Kzo9I GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
LmnI GCTCC 2 cut(s) 16, 362
Lsp1109I GCAGC 7 cut(s) 268, 271, 481, 484, 508, 511, 1259
LweI GCATC 2 cut(s) 132, 1260
MaeI CTAG 5 cut(s) 551, 633, 741, 959, 1418
MaeII ACGT 1 cut(s) 856
MaeIII GTNAC 1 cut(s) 1324
MalI GATC 7 cut(s) 186, 285, 410, 614, 653, 1174, 1353
MbiI CCGCTC 1 cut(s) 669
MboI GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
MboII GAAGA 5 cut(s) 16, 179, 317, 999, 1167
MflI RGATCY 1 cut(s) 1351
MhlI GDGCHC 4 cut(s) 83, 208, 523, 938
MluCI AATT 8 cut(s) 264, 427, 505, 726, 948, 1089, 1363, 1425
MlyI GAGTC 2 cut(s) 1174, 1313
MmeI TCCRAC 2 cut(s) 1066, 1402
Mph1103I ATGCAT 1 cut(s) 1253
MseI TTAA 9 cut(s) 20, 33, 102, 195, 564, 1106, 1119, 1362, 1466
MslI CAYNNNNRTG 1 cut(s) 153
MspCI CTTAAG 1 cut(s) 1465
MspI CCGG 2 cut(s) 53, 806
Mva1269I GAATGC 3 cut(s) 160, 383, 748
MwoI GCNNNNNNNGC 1 cut(s) 629
NdeII GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
NlaIII CATG 5 cut(s) 290, 659, 705, 997, 1165
NsiI ATGCAT 1 cut(s) 1253
NspV TTCGAA 1 cut(s) 115
PaqCI CACCTGC 1 cut(s) 614
PctI GAATGC 3 cut(s) 160, 383, 748
PfeI GAWTC 3 cut(s) 117, 123, 355
PkrI GCNGC 7 cut(s) 258, 261, 471, 474, 498, 501, 1274
PleI GAGTC 2 cut(s) 1173, 1312
PpsI GAGTC 2 cut(s) 1173, 1312
PsiI TTATAA 1 cut(s) 510
PspFI CCCAGC 2 cut(s) 365, 1097
PspPI GGNCC 1 cut(s) 953
PstI CTGCAG 1 cut(s) 838
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 1 cut(s) 1351
RsaI GTAC 2 cut(s) 333, 920
RsaNI GTAC 2 cut(s) 332, 919
RseI CAYNNNNRTG 1 cut(s) 153
SaqAI TTAA 9 cut(s) 20, 33, 102, 195, 564, 1106, 1119, 1362, 1466
SatI GCNGC 7 cut(s) 257, 260, 470, 473, 497, 500, 1273
Sau3AI GATC 7 cut(s) 184, 283, 408, 612, 651, 1172, 1351
Sau96I GGNCC 1 cut(s) 953
SchI GAGTC 2 cut(s) 1174, 1313
SduI GDGCHC 4 cut(s) 83, 208, 523, 938
SfaNI GCATC 2 cut(s) 132, 1260
SfcI CTRYAG 2 cut(s) 399, 834
SfuI TTCGAA 1 cut(s) 115
SinI GGWCC 1 cut(s) 953
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 1465
SmoI CTYRAG 1 cut(s) 1465
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 8 cut(s) 264, 427, 505, 726, 948, 1089, 1363, 1425
SsiI CCGC 2 cut(s) 237, 669
SspI AATATT 1 cut(s) 514
SspMI CTAG 5 cut(s) 551, 633, 741, 959, 1418
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 4 cut(s) 918, 1056, 1330, 1393
TaiI ACGT 1 cut(s) 859
TaqI TCGA 3 cut(s) 115, 163, 1171
TasI AATT 8 cut(s) 264, 427, 505, 726, 948, 1089, 1363, 1425
TfiI GAWTC 3 cut(s) 117, 123, 355
Tru1I TTAA 9 cut(s) 20, 33, 102, 195, 564, 1106, 1119, 1362, 1466
Tru9I TTAA 9 cut(s) 20, 33, 102, 195, 564, 1106, 1119, 1362, 1466
TscAI CASTG 1 cut(s) 1326
TseI GCWGC 7 cut(s) 256, 259, 469, 472, 496, 499, 1272
TspDTI ATGAA 8 cut(s) 17, 78, 102, 144, 233, 378, 449, 1419
TspRI CASTG 1 cut(s) 1326
Vha464I CTTAAG 1 cut(s) 1465
VneI GTGCAC 2 cut(s) 204, 519
VpaK11BI GGWCC 1 cut(s) 953
XapI RAATTY 1 cut(s) 1425
XbaI TCTAGA 1 cut(s) 958
XspI CTAG 5 cut(s) 551, 633, 741, 959, 1418
Zsp2I ATGCAT 1 cut(s) 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.