Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0013919

Physical Location & Seq

Genomic Coordinates Forward (+)

44221336 .. 44223908

Gene Structure 2573 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG176300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGCACACATTCTGCATTCCAATATTAACATCTTCTGTGTTCACAGTATATCTAGATGAGCCCGTATGGGAGTTCTCTGCAGCAGAACAAATCATTCTGCATAAGATCAAAGCACCTACAGATCAAATGGCACCTTTGGCTTTCCTTCTGCTATTCCATCTCCCTGGTTTTTCAGTTGCTGAGATTTCGAGCAAAATTGGCATAAACTATGGGCAGGTAGGGAACAATTTGCCTTCTCCAAGCCGGTCCATCGAGCGCATCAAATCCATGAAAATAGGGCGTGTGAAGCTCTATGATGCCAACCCGGAAATTTTAAGACTCCTATCCGGAACCAATATCTCAGTCTCCATCATGGTCCCAAACAATGAAATCACTGGCATTGCCTCAGACCAATCCAAAGCAGATGAATGGGTCAGAAACAATGTCCTCCCCTACTACCCCAAAACCATGATCCGGTACCTCCTCGTAGGCAATGAAATCCTCAGCTATTTCTCGGACCAAGATCGCCAAATATGGCACGATCTAGTCCCGGCCATGACTAAAATCAAGTATTCTCTCAAATCCAAAAATATCACTAACATCAAAATAGGTACCCCATTAGCAATGGACGTGGTTCAAACGAGTTTCCCACCATCAAGTGGCTCTTTCCGATCCGATATCTCGGAAACAGTCATGGTTCCCTTGGTCAGATTCTTGAACCGGACCAAGTCATTTTTCTTCATTGATGTGTACCCTTTTTTCCCATGGTCCTCAAACCCCAAAGACATTAGCCTAGACTATGCATTATTGAAAAACATTAGTAACAACAGTACTCTACGGTATACTGACCCAGGAAGTGGTTTGGTCTATACCAATCTTCTGGACCAAATGCTTGACTCCTTGATCTTCGCCATGACCAAGCTCGGCTACCCAAACATCCGGCTTTTGATAGCGGAGACTGGGTGGCCAAACTCCAGCAGTGAGCATAACCAACCGGGGGCTAATGTACACAATGCCGCCACATACAACCGAAATTTGGTACAAAAAATTGTTACTAAGCCAACAATTGGGACACCAGCGAGGCCTGGTGTAGTGATACCAACCTTCATATTTGCATTGTATGATGAAAATCAGAAGGGTGGGCCTGGAACAGAGAGGCACTGGGGATTGTTGTACGCTGATGGAACTCCGGTTTATGATATTGACTTGACGGGGAAGCGATCGGCAAATGACTACGAACCATTGCCGGAGAAGAGGCACTTCATAAAGGGAATTCATTACGACGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001871 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010208 GO:0010584 GO:0010927 GO:0016020 GO:0016043 GO:0016787 GO:0016798 GO:0022414 GO:0022607 GO:0030198 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0044085 GO:0044425 GO:0044459 GO:0044464 GO:0045229 GO:0046658 GO:0048229 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0061458 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

422

Amino Acids

47.4

Weight (kDa)

7.77

Isoelectric Point (pI)

44.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	66 - 395	3.2e-99	Glycosyl hydrolases family 17

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013919)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23770 AT4G14080
fragaria_vesca	FvH4_4g13120
malus_domestica	MD04G1019000.v1.1
prunus_persica	Prupe.1G168300_v2.0.a1
pyrus_communis	pycom04g01520
rosa_chinensis	RchiOBHm_Chr4g0412901
rosa_laevigata	RLG00000008249
rosa_multiflora	Rmu_sc0005621.1_g000022 Rmu_sc0012279.1_g000004
rosa_roxburghii	Rroxscaffold_5G00357140
rosa_rugosa	Rorug04G0117400
rosa_samantha	Rh4AG176300 Rh4BG176700 Rh4CG187300 Rh4DG173600
rosa_wichuraiana	Rw4G014800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	205
Acc65I	GGTACC	2 cut(s)	456, 590
AccB1I	GGYRCC	3 cut(s)	130, 456, 590
AccB7I	CCANNNNNTGG	3 cut(s)	638, 836, 1046
AccI	GTMKAC	1 cut(s)	821
AccIII	TCCGGA	1 cut(s)	326
AciI	CCGC	2 cut(s)	932, 996
AclWI	GGATC	2 cut(s)	445, 645
AcoI	YGGCCR	2 cut(s)	531, 944
AcsI	RAATTY	3 cut(s)	309, 1012, 1251
AfaI	GTAC	7 cut(s)	458, 592, 731, 811, 987, 1020, 1154
AfiI	CCNNNNNNNGG	6 cut(s)	453, 638, 836, 976, 1015, 1046
AgsI	TTSAA	3 cut(s)	617, 697, 790
AjiI	CACGTC	1 cut(s)	610
AjnI	CCWGG	4 cut(s)	163, 829, 1063, 1123
AjuI	GAANNNNNNNTTGG	2 cut(s)	698, 730
AluBI	AGCT	3 cut(s)	289, 486, 901
AluI	AGCT	3 cut(s)	289, 486, 901
Alw26I	GTCTC	2 cut(s)	349, 929
AlwI	GGATC	2 cut(s)	445, 645
AlwNI	CAGNNNCTG	1 cut(s)	179
Aor13HI	TCCGGA	1 cut(s)	326
AoxI	GGCC	4 cut(s)	531, 944, 1061, 1121
ApeKI	GCWGC	1 cut(s)	80
ApoI	RAATTY	3 cut(s)	309, 1012, 1251
Asp718I	GGTACC	2 cut(s)	456, 590
AspLEI	GCGC	1 cut(s)	258
AspS9I	GGNCC	7 cut(s)	246, 355, 496, 702, 747, 862, 1121
AsuC2I	CCSGG	3 cut(s)	305, 530, 975
AvaII	GGWCC	6 cut(s)	246, 355, 496, 702, 747, 862
BalI	TGGCCA	1 cut(s)	946
BanI	GGYRCC	3 cut(s)	130, 456, 590
BanII	GRGCYC	1 cut(s)	63
BbvCI	CCTCAGC	1 cut(s)	482
BbvI	GCAGC	1 cut(s)	92
BccI	CCATC	5 cut(s)	165, 257, 356, 640, 1154
BciT130I	CCWGG	4 cut(s)	165, 831, 1065, 1125
BcnI	CCSGG	3 cut(s)	305, 530, 975
BcoDI	GTCTC	2 cut(s)	349, 929
BfaI	CTAG	3 cut(s)	53, 524, 773
BfmI	CTRYAG	2 cut(s)	78, 117
BfuAI	ACCTGC	1 cut(s)	205
BisI	GCNGC	2 cut(s)	81, 996
BlsI	GCNGC	2 cut(s)	82, 997
BmcAI	AGTACT	1 cut(s)	811
Bme1390I	CCNGG	7 cut(s)	165, 305, 530, 831, 975, 1065, 1125
Bme18I	GGWCC	6 cut(s)	246, 355, 496, 702, 747, 862
BmgBI	CACGTC	1 cut(s)	610
BmgT120I	GGNCC	7 cut(s)	246, 355, 496, 702, 747, 862, 1121
BmiI	GGNNCC	6 cut(s)	132, 331, 357, 458, 592, 678
BmrFI	CCNGG	7 cut(s)	165, 305, 530, 831, 975, 1065, 1125
BmrI	ACTGGG	2 cut(s)	948, 1150
BmsI	GCATC	2 cut(s)	267, 286
BmuI	ACTGGG	2 cut(s)	948, 1150
BpmI	CTGGAG	1 cut(s)	937
Bpu10I	CCTNAGC	1 cut(s)	482
BpuMI	CCSGG	3 cut(s)	305, 530, 975
BsaBI	GATNNNNATC	1 cut(s)	1107
BsaJI	CCNNGG	5 cut(s)	163, 681, 743, 829, 974
BsaWI	WCCGGW	4 cut(s)	326, 453, 699, 1168
BsaXI	ACNNNNNCTCC	2 cut(s)	926, 956
Bsc4I	CCNNNNNNNGG	6 cut(s)	453, 638, 836, 976, 1015, 1046
Bse118I	RCCGGY	1 cut(s)	243
Bse1I	ACTGG	3 cut(s)	379, 943, 1145
Bse3DI	GCAATG	4 cut(s)	378, 478, 609, 1220
Bse8I	GATNNNNATC	1 cut(s)	1107
BseAI	TCCGGA	1 cut(s)	326
BseBI	CCWGG	4 cut(s)	165, 831, 1065, 1125
BseDI	CCNNGG	5 cut(s)	163, 681, 743, 829, 974
BseGI	GGATG	1 cut(s)	915
BseJI	GATNNNNATC	1 cut(s)	1107
BseLI	CCNNNNNNNGG	6 cut(s)	453, 638, 836, 976, 1015, 1046
BseMI	GCAATG	4 cut(s)	378, 478, 609, 1220
BseMII	CTCAG	4 cut(s)	171, 354, 399, 496
BseNI	ACTGG	3 cut(s)	379, 943, 1145
BseRI	GAGGAG	1 cut(s)	452
BseXI	GCAGC	1 cut(s)	92
Bsh1285I	CGRYCG	1 cut(s)	1202
BshFI	GGCC	4 cut(s)	533, 946, 1063, 1123
BshNI	GGYRCC	3 cut(s)	130, 456, 590
BsiEI	CGRYCG	1 cut(s)	1202
BslFI	GGGAC	3 cut(s)	341, 512, 1063
BslI	CCNNNNNNNGG	6 cut(s)	453, 638, 836, 976, 1015, 1046
BsmAI	GTCTC	2 cut(s)	349, 929
BsmFI	GGGAC	3 cut(s)	341, 512, 1063
BsmI	GAATGC	1 cut(s)	15
BsnI	GGCC	4 cut(s)	533, 946, 1063, 1123
Bsp1286I	GDGCHC	1 cut(s)	63
Bsp13I	TCCGGA	1 cut(s)	326
Bsp1407I	TGTACA	1 cut(s)	985
Bsp143I	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
Bsp19I	CCATGG	1 cut(s)	743
BspACI	CCGC	2 cut(s)	932, 996
BspANI	GGCC	4 cut(s)	533, 946, 1063, 1123
BspCNI	CTCAG	4 cut(s)	172, 353, 398, 495
BspEI	TCCGGA	1 cut(s)	326
BspLI	GGNNCC	6 cut(s)	132, 331, 357, 458, 592, 678
BspMAI	CTGCAG	1 cut(s)	82
BspMI	ACCTGC	1 cut(s)	205
BspPI	GGATC	2 cut(s)	445, 645
BspT107I	GGYRCC	3 cut(s)	130, 456, 590
BsrDI	GCAATG	4 cut(s)	378, 478, 609, 1220
BsrFI	RCCGGY	1 cut(s)	243
BsrGI	TGTACA	1 cut(s)	985
BsrI	ACTGG	3 cut(s)	379, 943, 1145
BssAI	RCCGGY	1 cut(s)	243
BssECI	CCNNGG	5 cut(s)	163, 681, 743, 829, 974
BssMI	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
BssNAI	GTATAC	1 cut(s)	822
BssT1I	CCWWGG	2 cut(s)	681, 743
Bst1107I	GTATAC	1 cut(s)	822
Bst2UI	CCWGG	4 cut(s)	165, 831, 1065, 1125
Bst4CI	ACNGT	4 cut(s)	46, 670, 809, 819
Bst6I	CTCTTC	1 cut(s)	1226
BstAUI	TGTACA	1 cut(s)	985
BstDEI	CTNAG	5 cut(s)	180, 340, 385, 482, 1035
BstDSI	CCRYGG	1 cut(s)	743
BstF5I	GGATG	1 cut(s)	915
BstHHI	GCGC	1 cut(s)	258
BstKTI	GATC	8 cut(s)	108, 124, 453, 505, 523, 653, 885, 1202
BstMAI	GTCTC	2 cut(s)	349, 929
BstMBI	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
BstMCI	CGRYCG	1 cut(s)	1202
BstMWI	GCNNNNNNNGC	3 cut(s)	137, 198, 286
BstNI	CCWGG	4 cut(s)	165, 831, 1065, 1125
BstSCI	CCNGG	7 cut(s)	163, 303, 528, 829, 973, 1063, 1123
BstSFI	CTRYAG	2 cut(s)	78, 117
BstV1I	GCAGC	1 cut(s)	92
BstXI	CCANNNNNNTGG	2 cut(s)	164, 859
BstZ17I	GTATAC	1 cut(s)	822
BsuRI	GGCC	4 cut(s)	533, 946, 1063, 1123
BtgI	CCRYGG	1 cut(s)	743
BtrI	CACGTC	1 cut(s)	610
BtsCI	GGATG	1 cut(s)	915
BtsI	GCAGTG	1 cut(s)	964
BtsIMutI	CAGTG	3 cut(s)	372, 964, 1138
BveI	ACCTGC	1 cut(s)	205
CaiI	CAGNNNCTG	1 cut(s)	179
CfoI	GCGC	1 cut(s)	258
Cfr10I	RCCGGY	1 cut(s)	243
Cfr13I	GGNCC	7 cut(s)	246, 355, 496, 702, 747, 862, 1121
Csp6I	GTAC	7 cut(s)	457, 591, 730, 810, 986, 1019, 1153
CspCI	CAANNNNNGTGG	2 cut(s)	591, 626
CviAII	CATG	7 cut(s)	268, 352, 448, 535, 673, 744, 892
CviQI	GTAC	7 cut(s)	457, 591, 730, 810, 986, 1019, 1153
DdeI	CTNAG	5 cut(s)	180, 340, 385, 482, 1035
DpnI	GATC	8 cut(s)	107, 123, 452, 504, 522, 652, 884, 1201
DpnII	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
EaeI	YGGCCR	2 cut(s)	531, 944
Eam1104I	CTCTTC	1 cut(s)	1226
EarI	CTCTTC	1 cut(s)	1226
Eco130I	CCWWGG	2 cut(s)	681, 743
Eco147I	AGGCCT	1 cut(s)	1063
Eco24I	GRGCYC	1 cut(s)	63
Eco32I	GATATC	1 cut(s)	658
Eco47I	GGWCC	6 cut(s)	246, 355, 496, 702, 747, 862
EcoRI	GAATTC	1 cut(s)	1251
EcoRII	CCWGG	4 cut(s)	163, 829, 1063, 1123
EcoRV	GATATC	1 cut(s)	658
EcoT14I	CCWWGG	2 cut(s)	681, 743
EcoT22I	ATGCAT	1 cut(s)	784
EcoT38I	GRGCYC	1 cut(s)	63
ErhI	CCWWGG	2 cut(s)	681, 743
FaeI	CATG	7 cut(s)	271, 355, 451, 538, 676, 747, 895
FalI	AAGNNNNNCTT	4 cut(s)	628, 660, 1223, 1255
FaqI	GGGAC	3 cut(s)	341, 512, 1063
FatI	CATG	7 cut(s)	267, 351, 447, 534, 672, 743, 891
FblI	GTMKAC	1 cut(s)	821
Fnu4HI	GCNGC	2 cut(s)	81, 996
FokI	GGATG	1 cut(s)	902
FriOI	GRGCYC	1 cut(s)	63
Fsp4HI	GCNGC	2 cut(s)	81, 996
FspBI	CTAG	3 cut(s)	53, 524, 773
GlaI	GCGC	1 cut(s)	257
GluI	GCNGC	2 cut(s)	81, 996
GsuI	CTGGAG	1 cut(s)	937
HaeIII	GGCC	4 cut(s)	533, 946, 1063, 1123
HhaI	GCGC	1 cut(s)	258
Hin1II	CATG	7 cut(s)	271, 355, 451, 538, 676, 747, 895
Hin6I	GCGC	1 cut(s)	256
HinP1I	GCGC	1 cut(s)	256
HinfI	GANTC	3 cut(s)	318, 690, 875
Hpy166II	GTNNAC	4 cut(s)	42, 730, 822, 988
Hpy188I	TCNGA	8 cut(s)	388, 416, 496, 650, 655, 664, 689, 1113
Hpy188III	TCNNGA	4 cut(s)	53, 327, 694, 860
Hpy8I	GTNNAC	4 cut(s)	42, 730, 822, 988
Hpy99I	CGWCG	1 cut(s)	1265
HpyAV	CCTTC	4 cut(s)	155, 243, 1093, 1108
HpyCH4III	ACNGT	4 cut(s)	46, 670, 809, 819
HpyCH4IV	ACGT	1 cut(s)	609
HpyCH4V	TGCA	6 cut(s)	4, 15, 80, 100, 782, 1094
HpyF10VI	GCNNNNNNNGC	3 cut(s)	137, 198, 286
HpyF3I	CTNAG	5 cut(s)	180, 340, 385, 482, 1035
HpySE526I	ACGT	1 cut(s)	609
Hsp92II	CATG	7 cut(s)	271, 355, 451, 538, 676, 747, 895
HspAI	GCGC	1 cut(s)	256
Kpn2I	TCCGGA	1 cut(s)	326
KpnI	GGTACC	2 cut(s)	460, 594
Kzo9I	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
Lsp1109I	GCAGC	1 cut(s)	92
LweI	GCATC	2 cut(s)	267, 286
MaeI	CTAG	3 cut(s)	53, 524, 773
MaeII	ACGT	1 cut(s)	609
MaeIII	GTNAC	2 cut(s)	800, 1030
MalI	GATC	8 cut(s)	107, 123, 452, 504, 522, 652, 884, 1201
MboI	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
MboII	GAAGA	5 cut(s)	24, 709, 848, 877, 1243
MfeI	CAATTG	1 cut(s)	1044
MhlI	GDGCHC	1 cut(s)	63
MlsI	TGGCCA	1 cut(s)	946
MluCI	AATT	7 cut(s)	195, 226, 309, 1012, 1026, 1044, 1251
MluNI	TGGCCA	1 cut(s)	946
MlyI	GAGTC	2 cut(s)	312, 869
MnlI	CCTC	9 cut(s)	394, 437, 470, 473, 491, 760, 1053, 1128, 1227
Mox20I	TGGCCA	1 cut(s)	946
Mph1103I	ATGCAT	1 cut(s)	784
MroI	TCCGGA	1 cut(s)	326
MscI	TGGCCA	1 cut(s)	946
MseI	TTAA	2 cut(s)	26, 314
MslI	CAYNNNNRTG	1 cut(s)	725
Msp20I	TGGCCA	1 cut(s)	946
MspR9I	CCNGG	7 cut(s)	165, 305, 530, 831, 975, 1065, 1125
MunI	CAATTG	1 cut(s)	1044
Mva1269I	GAATGC	1 cut(s)	15
MvaI	CCWGG	4 cut(s)	165, 831, 1065, 1125
MwoI	GCNNNNNNNGC	3 cut(s)	137, 198, 286
NciI	CCSGG	3 cut(s)	305, 530, 975
NcoI	CCATGG	1 cut(s)	743
NdeII	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
NlaIII	CATG	7 cut(s)	271, 355, 451, 538, 676, 747, 895
NlaIV	GGNNCC	6 cut(s)	132, 331, 357, 458, 592, 678
NmeAIII	GCCGAG	1 cut(s)	882
NsiI	ATGCAT	1 cut(s)	784
PceI	AGGCCT	1 cut(s)	1063
PctI	GAATGC	1 cut(s)	15
PfeI	GAWTC	1 cut(s)	690
PflFI	GACNNNGTC	1 cut(s)	706
PflMI	CCANNNNNTGG	3 cut(s)	638, 836, 1046
PkrI	GCNGC	2 cut(s)	82, 997
Ple19I	CGATCG	1 cut(s)	1202
PleI	GAGTC	2 cut(s)	312, 869
PpsI	GAGTC	2 cut(s)	312, 869
Psp6I	CCWGG	4 cut(s)	163, 829, 1063, 1123
PspGI	CCWGG	4 cut(s)	163, 829, 1063, 1123
PspN4I	GGNNCC	6 cut(s)	132, 331, 357, 458, 592, 678
PspPI	GGNCC	7 cut(s)	246, 355, 496, 702, 747, 862, 1121
PstI	CTGCAG	1 cut(s)	82
PstNI	CAGNNNCTG	1 cut(s)	179
PsyI	GACNNNGTC	1 cut(s)	706
PvuI	CGATCG	1 cut(s)	1202
RsaI	GTAC	7 cut(s)	458, 592, 731, 811, 987, 1020, 1154
RsaNI	GTAC	7 cut(s)	457, 591, 730, 810, 986, 1019, 1153
RseI	CAYNNNNRTG	1 cut(s)	725
SaqAI	TTAA	2 cut(s)	26, 314
SatI	GCNGC	2 cut(s)	81, 996
Sau3AI	GATC	8 cut(s)	105, 121, 450, 502, 520, 650, 882, 1199
Sau96I	GGNCC	7 cut(s)	246, 355, 496, 702, 747, 862, 1121
ScaI	AGTACT	1 cut(s)	811
SchI	GAGTC	2 cut(s)	312, 869
ScrFI	CCNGG	7 cut(s)	165, 305, 530, 831, 975, 1065, 1125
SduI	GDGCHC	1 cut(s)	63
SfaNI	GCATC	2 cut(s)	267, 286
SfcI	CTRYAG	2 cut(s)	78, 117
SinI	GGWCC	6 cut(s)	246, 355, 496, 702, 747, 862
SmiMI	CAYNNNNRTG	1 cut(s)	725
Sse9I	AATT	7 cut(s)	195, 226, 309, 1012, 1026, 1044, 1251
SseBI	AGGCCT	1 cut(s)	1063
SsiI	CCGC	2 cut(s)	932, 996
SspI	AATATT	1 cut(s)	24
SspMI	CTAG	3 cut(s)	53, 524, 773
StuI	AGGCCT	1 cut(s)	1063
StyD4I	CCNGG	7 cut(s)	163, 303, 528, 829, 973, 1063, 1123
StyI	CCWWGG	2 cut(s)	681, 743
TaaI	ACNGT	4 cut(s)	46, 670, 809, 819
TaiI	ACGT	1 cut(s)	612
TaqI	TCGA	2 cut(s)	188, 252
TasI	AATT	7 cut(s)	195, 226, 309, 1012, 1026, 1044, 1251
TatI	WGTACW	2 cut(s)	809, 985
TauI	GCSGC	1 cut(s)	998
TfiI	GAWTC	1 cut(s)	690
Tru1I	TTAA	2 cut(s)	26, 314
Tru9I	TTAA	2 cut(s)	26, 314
TscAI	CASTG	3 cut(s)	379, 964, 1145
TseI	GCWGC	1 cut(s)	80
TspDTI	ATGAA	9 cut(s)	284, 381, 420, 489, 709, 1075, 1119, 1231, 1244
TspRI	CASTG	3 cut(s)	379, 964, 1145
Tth111I	GACNNNGTC	1 cut(s)	706
Van91I	CCANNNNNTGG	3 cut(s)	638, 836, 1046
VpaK11BI	GGWCC	6 cut(s)	246, 355, 496, 702, 747, 862
XapI	RAATTY	3 cut(s)	309, 1012, 1251
XbaI	TCTAGA	1 cut(s)	52
XmiI	GTMKAC	1 cut(s)	821
XspI	CTAG	3 cut(s)	53, 524, 773
ZrmI	AGTACT	1 cut(s)	811
Zsp2I	ATGCAT	1 cut(s)	784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.